Abstract:
:The epidermal growth factor receptor gene has been found to be amplified and rearranged in human glioblastomas in vivo. Here we present the sequence across a splice junction of aberrant epidermal growth factor receptor transcripts derived from corresponding and uniquely rearranged genes that are coamplified and coexpressed with non-rearranged epidermal growth factor receptor genes in six primary human glioblastomas. Each of these six tumors contains aberrant transcripts derived from identical splicing of exon 1 to exon 8 as a consequence of a deletion-rearrangement of the amplified gene, the extent of which is variable among these tumors. In spite of this intertumoral variability, each intragenic rearrangement results in loss of the same 801 coding bases (exons 2-7) and creation of a new codon at the novel splice site in their corresponding transcripts. These rearrangements do not, however, affect the mRNA sequence for the signal peptide, the first five codons, or the reading frame downstream of the rearrangement.
journal_name
Proc Natl Acad Sci U S Aauthors
Sugawa N,Ekstrand AJ,James CD,Collins VPdoi
10.1073/pnas.87.21.8602subject
Has Abstractpub_date
1990-11-01 00:00:00pages
8602-6issue
21eissn
0027-8424issn
1091-6490journal_volume
87pub_type
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