Heat-shock protein 70 from plant biofactories of recombinant antigens activate multiepitope-targeted immune responses.

abstract：:Zinc-finger nucleases (ZFNs) are artificial restriction enzymes, custom designed for induction of double-strand breaks (DSBs) at a specific locus. These DSBs may result in site-specific mutagenesis or homologous recombination at the repair site, depending on the DNA repair pathway that is used. These promising techniq...

journal_title：Plant biotechnology journal

authors： de Pater S,Neuteboom LW,Pinas JE,Hooykaas PJ,van der Zaal BJ

更新日期：2009-10-01 00:00:00

abstract：:Grain size, number and starch content are important determinants of grain yield and quality. One of the most important biological processes that determine these components is the carbon partitioning during the early grain filling, which requires the function of cell wall invertase. Here, we showed the constitutive exp...

journal_title：Plant biotechnology journal

authors： Li B,Liu H,Zhang Y,Kang T,Zhang L,Tong J,Xiao L,Zhang H

更新日期：2013-12-01 00:00:00

abstract：:The rice root system is important for growth. The crosstalk between auxin and cytokinin mediates root initiation and elongation. However, it remains unclear how the transcriptional network upstream of the auxin and cytokinin signalling pathways determines root development. Here, we observed that the knockdown of OsNAC...

journal_title：Plant biotechnology journal

authors： Mao C,He J,Liu L,Deng Q,Yao X,Liu C,Qiao Y,Li P,Ming F

更新日期：2020-02-01 00:00:00

abstract：:Receptor-like kinases (RLKs) are important components of plant innate immunity. Although recent studies have revealed that the RLK suppressor of BIR1-1 (SOBIR1) can interact with multiple receptor-like proteins and is required for resistance against fungal pathogens, how the signal is transduced and triggers immune re...

journal_title：Plant biotechnology journal

authors： Zhou Y,Sun L,Wassan GM,He X,Shaban M,Zhang L,Zhu L,Zhang X

更新日期：2019-01-01 00:00:00

abstract：:Modified fatty acids (mFA) have diverse uses; for example, cyclopropane fatty acids (CPA) are feedstocks for producing coatings, lubricants, plastics and cosmetics. The expression of mFA-producing enzymes in crop and model plants generally results in lower levels of mFA accumulation than in their natural-occurring sou...

journal_title：Plant biotechnology journal

authors： Yu XH,Cahoon RE,Horn PJ,Shi H,Prakash RR,Cai Y,Hearney M,Chapman KD,Cahoon EB,Schwender J,Shanklin J

更新日期：2018-04-01 00:00:00

abstract：:Glyphosate is a popular, systemic, broad-spectrum herbicide used in modern agriculture. Being a structural analog of phosphoenolpyruvate (PEP), it inhibits 5-enolpyruvylshikimate 3-phosphate synthase (EPSPS) which is responsible for the biosynthesis of aromatic amino acids and various aromatic secondary metabolites. T...

journal_title：Plant biotechnology journal

authors： Achary VMM,Sheri V,Manna M,Panditi V,Borphukan B,Ram B,Agarwal A,Fartyal D,Teotia D,Masakapalli SK,Agrawal PK,Reddy MK

更新日期：2020-12-01 00:00:00

abstract：:Porcine circovirus type 2 (PCV-2) is the main causative agent associated with a group of diseases collectively known as porcine circovirus-associated disease (PCAD). There is a significant economic strain on the global swine industry due to PCAD and the production of commercial PCV-2 vaccines is expensive. Plant expre...

journal_title：Plant biotechnology journal

authors： Gunter CJ,Regnard GL,Rybicki EP,Hitzeroth II

更新日期：2019-09-01 00:00:00

abstract：:To date, transgenic approaches to biofortify subsistence crops have been rather limited. This is particularly true for the starchy root crop cassava (Manihot esculenta Crantz). Cassava has one of the highest rates of CO(2) fixation and sucrose synthesis for any C3 plant, but rarely reaches its yield potentials in the ...

journal_title：Plant biotechnology journal

authors： Ihemere U,Arias-Garzon D,Lawrence S,Sayre R

更新日期：2006-07-01 00:00:00

abstract：:Peanut allergy is an IgE-mediated adverse reaction to a subset of proteins found in peanuts. Immunotherapy aims to desensitize allergic patients through repeated and escalating exposures for several months to years using extracts or flours. The complex mix of proteins and variability between preparations complicates i...

journal_title：Plant biotechnology journal

authors： Gregory JA,Shepley-McTaggart A,Umpierrez M,Hurlburt BK,Maleki SJ,Sampson HA,Mayfield SP,Berin MC

更新日期：2016-07-01 00:00:00

abstract：:The importance and urgency of providing humans and animals with quality proteins are reflected in the growing scientific and industrial interest in augmenting the nutritive value of the world's protein sources. Such nutritive value is determined by the protein content in 'essential amino acids', those that cannot be s...

journal_title：Plant biotechnology journal

authors： Beauregard M,Hefford MA

更新日期：2006-09-01 00:00:00

abstract：:Triterpenes are thirty-carbon compounds derived from the universal five-carbon prenyl precursors isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). Normally, triterpenes are synthesized via the mevalonate (MVA) pathway operating in the cytoplasm of eukaryotes where DMAPP is condensed with two IPPs to...

journal_title：Plant biotechnology journal

authors： Kempinski C,Jiang Z,Zinck G,Sato SJ,Ge Z,Clemente TE,Chappell J

更新日期：2019-02-01 00:00:00

abstract：:Following the molecular characterisation of functional disease resistance genes in recent years, methods to track and verify the integrity of multiple genes in varieties are needed for crop improvement through resistance stacking. Diagnostic resistance gene enrichment sequencing (dRenSeq) enables the high-confidence i...

journal_title：Plant biotechnology journal

authors： Armstrong MR,Vossen J,Lim TY,Hutten RCB,Xu J,Strachan SM,Harrower B,Champouret N,Gilroy EM,Hein I

更新日期：2019-02-01 00:00:00

abstract：:Recent report reviews and funding initiatives in the field of plant genomic research are considered in the context of their translation into practical and economic value via plant breeding. It is concluded that there is a deficit in investment and that a change in working styles towards knowledge sharing and connectiv...

journal_title：Plant biotechnology journal

authors： Hughes S

更新日期：2006-01-01 00:00:00

abstract：:The replacement of crude allergen extracts by selected allergens currently represents a major goal for the improvement of allergy diagnosis and immunotherapy. Indeed, the development of molecularly defined vaccines would facilitate both standardization and enhance batch-to-batch reproducibility as well as treatment sp...

journal_title：Plant biotechnology journal

authors： Lienard D,Tran Dinh O,van Oort E,Van Overtvelt L,Bonneau C,Wambre E,Bardor M,Cosette P,Didier-Laurent A,de Borne FD,Delon R,van Ree R,Moingeon P,Faye L,Gomord V

更新日期：2007-01-01 00:00:00

abstract：:Which factors limit metabolite accumulation in plant cells? Are theories on flux control effective at explaining the results? Many biotechnologists cling to the idea that every pathway has a rate limiting enzyme and target such enzymes first in order to modulate fluxes. This often translates into large effects on meta...

journal_title：Plant biotechnology journal

authors： Morandini P

更新日期：2013-02-01 00:00:00

abstract：:Food supplementation with the conditionally essential amino acid arginine (Arg) has been shown to have nutritional benefits. Degradation of cyanophycin (CGP), a peptide polymer used for nitrogen storage by cyanobacteria, requires cyanophycinase (CGPase) and results in the release of β-aspartic acid (Asp)-Arg dipeptide...

journal_title：Plant biotechnology journal

authors： Ponndorf D,Ehmke S,Walliser B,Thoss K,Unger C,Görs S,Daş G,Metges CC,Broer I,Nausch H

更新日期：2017-05-01 00:00:00

abstract：:We constructed a novel autonomously replicating gene expression shuttle vector, with the aim of developing a system for transiently expressing proteins at levels useful for commercial production of vaccines and other proteins in plants. The vector, pRIC, is based on the mild strain of the geminivirus Bean yellow dwarf...

journal_title：Plant biotechnology journal

authors： Regnard GL,Halley-Stott RP,Tanzer FL,Hitzeroth II,Rybicki EP

更新日期：2010-01-01 00:00:00

abstract：:There is an urgent need to provide effective anti-HIV microbicides to resource-poor areas worldwide. Some of the most promising microbicide candidates are biotherapeutics targeting viral entry. To provide biotherapeutics to poorer areas, it is vital to reduce the cost. Here, we report the production of biologically ac...

journal_title：Plant biotechnology journal

authors： O'Keefe BR,Murad AM,Vianna GR,Ramessar K,Saucedo CJ,Wilson J,Buckheit KW,da Cunha NB,Araújo AC,Lacorte CC,Madeira L,McMahon JB,Rech EL

更新日期：2015-09-01 00:00:00

abstract：:The assembly of a reference genome sequence of bread wheat is challenging due to its specific features such as the genome size of 17 Gbp, polyploid nature and prevalence of repetitive sequences. BAC-by-BAC sequencing based on chromosomal physical maps, adopted by the International Wheat Genome Sequencing Consortium as...

journal_title：Plant biotechnology journal

authors： Staňková H,Hastie AR,Chan S,Vrána J,Tulpová Z,Kubaláková M,Visendi P,Hayashi S,Luo M,Batley J,Edwards D,Doležel J,Šimková H

更新日期：2016-07-01 00:00:00

abstract：:2-Acetyl-1-pyrroline (2AP), the volatile compound that provides the 'popcorn-like' aroma in a large variety of cereal and food products, is widely found in nature. Deficiency in amino aldehyde dehydrogenase (AMADH) was previously shown to be the likely cause of 2AP biosynthesis in rice (Oryza sativa L.). In this study...

journal_title：Plant biotechnology journal

authors： Arikit S,Yoshihashi T,Wanchana S,Uyen TT,Huong NT,Wongpornchai S,Vanavichit A

更新日期：2011-01-01 00:00:00

abstract：:Salicylic acid (SA) is an essential hormone for plant defence and development. SA perception is usually measured by counting the number of pathogens that grow in planta upon an exogenous application of the hormone. A biological SA perception model based on plant fresh weight reduction caused by disease resistance in A...

journal_title：Plant biotechnology journal

authors： Canet JV,Dobón A,Ibáñez F,Perales L,Tornero P

更新日期：2010-02-01 00:00:00

abstract：:Cadmium (Cd) is a widespread soil contaminant threatening human health. As an ideal energy plant, sweet sorghum (Sorghum bicolor (L.) Moench) has great potential in phytoremediation of Cd-polluted soils, although the molecular mechanisms are largely unknown. In this study, key factors responsible for differential Cd a...

journal_title：Plant biotechnology journal

authors： Feng J,Jia W,Lv S,Bao H,Miao F,Zhang X,Wang J,Li J,Li D,Zhu C,Li S,Li Y

更新日期：2018-02-01 00:00:00

abstract：:The hexaploid wheat (Triticum aestivum) adult plant resistance gene, Lr34/Yr18/Sr57/Pm38/Ltn1, provides broad-spectrum resistance to wheat leaf rust (Lr34), stripe rust (Yr18), stem rust (Sr57) and powdery mildew (Pm38) pathogens, and has remained effective in wheat crops for many decades. The partial resistance provi...

journal_title：Plant biotechnology journal

authors： Rinaldo A,Gilbert B,Boni R,Krattinger SG,Singh D,Park RF,Lagudah E,Ayliffe M

更新日期：2017-07-01 00:00:00

abstract：:The intensification of agriculture and the development of synthetic insecticides enabled worldwide grain production to more than double in the last third of the 20th century. However, the heavy dependence and, in some cases, overuse of insecticides has been responsible for negative environmental and ecological impacts...

journal_title：Plant biotechnology journal

authors： Catarino R,Ceddia G,Areal FJ,Park J

更新日期：2015-06-01 00:00:00

abstract：:Heavy metal accumulation in the environment poses great risks to flora and fauna. However, monitoring sites prone to accumulation poses scale and economic challenges. In this study, we present and test a method for monitoring these sites using fluorescent resonance energy transfer (FRET) change in response to zinc (Zn...

journal_title：Plant biotechnology journal

authors： Adams JP,Adeli A,Hsu CY,Harkess RL,Page GP,Depamphilis CW,Schultz EB,Yuceer C

更新日期：2012-02-01 00:00:00

abstract：:It is widely recognized that biofuel production from lignocellulosic materials is limited by inadequate technology to efficiently and economically release fermentable sugars from the complex multi-polymeric raw materials. Therefore, endoglucanases, exoglucanase, pectate lyases, cutinase, swollenin, xylanase, acetyl xy...

journal_title：Plant biotechnology journal

authors： Verma D,Kanagaraj A,Jin S,Singh ND,Kolattukudy PE,Daniell H

更新日期：2010-04-01 00:00:00

abstract：:Bacterial wilt, caused by Ralstonia solanacearum, is a devastating disease affecting over 350 plant species. A few peanut cultivars were found to possess stable and durable bacterial wilt resistance (BWR). Genomics-assisted breeding can accelerate the process of developing resistant cultivars by using diagnostic marke...

journal_title：Plant biotechnology journal

authors： Luo H,Pandey MK,Khan AW,Wu B,Guo J,Ren X,Zhou X,Chen Y,Chen W,Huang L,Liu N,Lei Y,Liao B,Varshney RK,Jiang H

更新日期：2019-12-01 00:00:00

abstract：:Nanobodies® (VHHs) provide powerful tools in therapeutic and biotechnological applications. Nevertheless, for some applications, bivalent antibodies perform much better, and for this, an Fc chain can be fused to the VHH domain, resulting in a bivalent homodimeric VHH-Fc complex. However, the production of bivalent ant...

journal_title：Plant biotechnology journal

authors： De Buck S,Nolf J,De Meyer T,Virdi V,De Wilde K,Van Lerberge E,Van Droogenbroeck B,Depicker A

更新日期：2013-10-01 00:00:00

abstract：:The plant pathogen, Pseudomonas syringae (Ps), together with related Ps species, infects and attacks a wide range of agronomically important crops, including tomato, kiwifruit, pepper, olive and soybean, causing economic losses. Currently, chemicals and introduced resistance genes are used to protect plants against th...

journal_title：Plant biotechnology journal

authors： Rooney WM,Grinter RW,Correia A,Parkhill J,Walker DC,Milner JJ

更新日期：2020-05-01 00:00:00

abstract：:Starch is an important renewable raw material with an increasing number of applications. Several attempts have been made to obtain plants that produce modified versions of starch or higher starch yield. Most of the approaches designed to increase the levels of starch have focused on the increment of the amount of ADP-...

journal_title：Plant biotechnology journal

authors： Gámez-Arjona FM,Li J,Raynaud S,Baroja-Fernández E,Muñoz FJ,Ovecka M,Ragel P,Bahaji A,Pozueta-Romero J,Mérida Á

更新日期：2011-12-01 00:00:00