Heat-shock protein 70 from plant biofactories of recombinant antigens activate multiepitope-targeted immune responses.

abstract：:Dengue fever is a mosquito (Aedes aegypti) -transmitted viral disease that is endemic in more than 125 countries around the world. There are four serotypes of the dengue virus (DENV 1-4) and a safe and effective dengue vaccine must provide protection against all four serotypes. To date, the first vaccine, Dengvaxia (C...

journal_title：Plant biotechnology journal

authors： van Eerde A,Gottschamel J,Bock R,Hansen KEA,Munang'andu HM,Daniell H,Liu Clarke J

更新日期：2019-07-01 00:00:00

abstract：:In the last 20 years, stem rust caused by the fungus Puccinia graminis f. sp. tritici (Pgt), has re-emerged as a major threat to wheat and barley production in Africa and Europe. In contrast to wheat with 60 designated stem rust (Sr) resistance genes, barley's genetic variation for stem rust resistance is very narrow ...

journal_title：Plant biotechnology journal

authors： Hatta MAM,Arora S,Ghosh S,Matny O,Smedley MA,Yu G,Chakraborty S,Bhatt D,Xia X,Steuernagel B,Richardson T,Mago R,Lagudah ES,Patron NJ,Ayliffe M,Rouse MN,Harwood WA,Periyannan S,Steffenson BJ,Wulff BBH

更新日期：2020-08-03 00:00:00

abstract：:Genetic modification of Zn/Cd accumulation in roots and shoots for biofortification or phytoremediation is a focus of this manuscript. We expressed AtHMA4 (a P(₁B) ATPase involved in Zn and Cd transport), AtHMA4-trunc (lacking the C-terminal region) and AtHMA4-C terminus (the C-terminal region alone) in tobacco under ...

journal_title：Plant biotechnology journal

authors： Siemianowski O,Mills RF,Williams LE,Antosiewicz DM

更新日期：2011-01-01 00:00:00

abstract：:Recently, CRISPR-Cas12a (Cpf1) from Prevotella and Francisella was engineered to modify plant genomes. In this report, we employed CRISPR-LbCas12a (LbCpf1), which is derived from Lachnospiraceae bacterium ND2006, to edit a citrus genome for the first time. First, LbCas12a was used to modify the CsPDS gene successfully...

journal_title：Plant biotechnology journal

authors： Jia H,Orbović V,Wang N

更新日期：2019-10-01 00:00:00

abstract：:Although conifers are of immense ecological and economic value, bioengineering of their chloroplasts remains undeveloped. Understanding the chloroplast genomic organization of conifers can facilitate their bioengineering. Members of the conifer II clade (or cupressophytes) are highly diverse in both morphologic featur...

journal_title：Plant biotechnology journal

authors： Wu CS,Chaw SM

更新日期：2014-04-01 00:00:00

abstract：:Recent developments of tools for targeted genome modification have led to new concepts in how multiple traits can be combined. Targeted genome modification is based on the use of nucleases with tailor-made specificities to introduce a DNA double-strand break (DSB) at specific target loci. A re-engineered meganuclease ...

journal_title：Plant biotechnology journal

authors： D'Halluin K,Vanderstraeten C,Van Hulle J,Rosolowska J,Van Den Brande I,Pennewaert A,D'Hont K,Bossut M,Jantz D,Ruiter R,Broadhvest J

更新日期：2013-10-01 00:00:00

abstract：:DNA recombination reactions (site-specific and homologous) were monitored in the progeny of transgenic maize plants by bringing together two recombination substrates (docking sites and shuttle vectors) in the zygotes. In one combination of transgenic events, the recombination marker gene (yellow fluorescent protein ge...

journal_title：Plant biotechnology journal

authors： Djukanovic V,Orczyk W,Gao H,Sun X,Garrett N,Zhen S,Gordon-Kamm W,Barton J,Lyznik LA

更新日期：2006-05-01 00:00:00

abstract：:Orobanche spp. (broomrape) are parasitic plants which subsist on the roots of a wide range of hosts, including tomato, causing severe losses in yield quality and quantity. Large amounts of mannitol accumulate in this parasitic weed during development. Mannose 6-phosphate reductase (M6PR) is a key enzyme in mannitol bi...

journal_title：Plant biotechnology journal

authors： Aly R,Cholakh H,Joel DM,Leibman D,Steinitz B,Zelcer A,Naglis A,Yarden O,Gal-On A

更新日期：2009-08-01 00:00:00

abstract：:Triterpenes are thirty-carbon compounds derived from the universal five-carbon prenyl precursors isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). Normally, triterpenes are synthesized via the mevalonate (MVA) pathway operating in the cytoplasm of eukaryotes where DMAPP is condensed with two IPPs to...

journal_title：Plant biotechnology journal

authors： Kempinski C,Jiang Z,Zinck G,Sato SJ,Ge Z,Clemente TE,Chappell J

更新日期：2019-02-01 00:00:00

abstract：:The 1980s and 1990s saw a major expansion of biotechnology into new areas of science including genomics and recombinant technologies. This was coupled to the widespread emergence of academics into the commercial sector as they were encouraged to spin out companies or commercialize their intellectual property. There we...

journal_title：Plant biotechnology journal

authors： Paul MJ,Thangaraj H,Ma JK

更新日期：2015-10-01 00:00:00

abstract：:Downy mildew (Plasmopara halstedii (Farl.) Berlese et de Toni) is a serious foliar pathogen of cultivated sunflower (Helianthus annuus L.). Genetic resistance is conditioned by several linked downy mildew resistance gene specificities in the HaRGC1 cluster of TIR-NBS-LRR resistance gene candidates (RGCs) on linkage gr...

journal_title：Plant biotechnology journal

authors： Slabaugh MB,Yu JK,Tang S,Heesacker A,Hu X,Lu G,Bidney D,Han F,Knapp SJ

更新日期：2003-05-01 00:00:00

abstract：:Single-chain variable fragments (scFvs), as well as other recombinant antibodies, have become powerful tools for therapy and analysis in both human and veterinary medicine. Transgenic plants have been developed as an efficient production system for these recombinant proteins, achieving high yields at moderate cost. Se...

journal_title：Plant biotechnology journal

authors： Scheller J,Leps M,Conrad U

更新日期：2006-03-01 00:00:00

abstract：:Glycolate is produced in autotrophic cells under high temperatures and Ci -limitation via oxygenation of ribulose-1,5-bisphosphate. In unicellular algae, glycolate is lost via excretion or metabolized via the C2 cycle by consuming reductants, ATP and CO2 emission (photorespiration). Therefore, photorespiration is an i...

journal_title：Plant biotechnology journal

authors： Taubert A,Jakob T,Wilhelm C

更新日期：2019-08-01 00:00:00

abstract：:Bacterial wilt, caused by Ralstonia solanacearum, is a devastating disease affecting over 350 plant species. A few peanut cultivars were found to possess stable and durable bacterial wilt resistance (BWR). Genomics-assisted breeding can accelerate the process of developing resistant cultivars by using diagnostic marke...

journal_title：Plant biotechnology journal

authors： Luo H,Pandey MK,Khan AW,Wu B,Guo J,Ren X,Zhou X,Chen Y,Chen W,Huang L,Liu N,Lei Y,Liao B,Varshney RK,Jiang H

更新日期：2019-12-01 00:00:00

abstract：:Differences in gene sequences, many of which are single nucleotide polymorphisms, underlie some of the most important traits in plants. With humanity facing significant challenges to increase global agricultural productivity, there is an urgent need to accelerate the development of these traits in plants. oligonucleot...

journal_title：Plant biotechnology journal

authors： Sauer NJ,Mozoruk J,Miller RB,Warburg ZJ,Walker KA,Beetham PR,Schöpke CR,Gocal GF

更新日期：2016-02-01 00:00:00

abstract：:Glycine betaine plays an important role in some plants, including maize, in conditions of abiotic stress, but different maize varieties vary in their capacity to accumulate glycine betaine. An elite maize inbred line DH4866 was transformed with the betA gene from Escherichia coli encoding choline dehydrogenase (EC 1.1...

journal_title：Plant biotechnology journal

authors： Quan R,Shang M,Zhang H,Zhao Y,Zhang J

更新日期：2004-11-01 00:00:00

abstract：:Antioxidants are protective molecules that neutralize reactive oxygen species and prevent oxidative damage to cellular components such as membranes, proteins and nucleic acids, therefore reducing the rate of cell death and hence the effects of ageing and ageing-related diseases. The fortification of food with antioxid...

journal_title：Plant biotechnology journal

authors： Zhu C,Sanahuja G,Yuan D,Farré G,Arjó G,Berman J,Zorrilla-López U,Banakar R,Bai C,Pérez-Massot E,Bassie L,Capell T,Christou P

更新日期：2013-02-01 00:00:00

abstract：:Phytochrome-interacting factors (PIFs) play major roles in regulating plant growth and development, but their roles in drought stress remain elusive. Here, we cloned and characterized a maize (Zea mays) PIF transcription factor, ZmPIF1. The expression level of ZmPIF1 was significantly induced by independent drought an...

journal_title：Plant biotechnology journal

authors： Gao Y,Wu M,Zhang M,Jiang W,Ren X,Liang E,Zhang D,Zhang C,Xiao N,Li Y,Dai Y,Chen J

更新日期：2018-07-01 00:00:00

abstract：:Transgenic rice accumulating the modified major Japanese cedar pollen allergens, Cryptomeria japonica 1 (Cry j 1) and Cryptomeria japonica 2 (Cry j 2), which were deconstructed by fragmentation and shuffling, respectively, in the edible part of the seed was generated by transformation of a good-tasting rice variety, '...

journal_title：Plant biotechnology journal

authors： Wakasa Y,Takagi H,Hirose S,Yang L,Saeki M,Nishimura T,Kaminuma O,Hiroi T,Takaiwa F

更新日期：2013-01-01 00:00:00

abstract：:Sumoylation is a posttranslational regulatory process in higher eukaryotes modifying substrate proteins through conjugation of small ubiquitin-related modifiers (SUMOs). Sumoylation modulates protein stability, subcellular localization and activity; thus, it regulates most cellular functions including response to envi...

journal_title：Plant biotechnology journal

authors： Li Z,Hu Q,Zhou M,Vandenbrink J,Li D,Menchyk N,Reighard S,Norris A,Liu H,Sun D,Luo H

更新日期：2013-05-01 00:00:00

abstract：:The hexaploid wheat (Triticum aestivum) adult plant resistance gene, Lr34/Yr18/Sr57/Pm38/Ltn1, provides broad-spectrum resistance to wheat leaf rust (Lr34), stripe rust (Yr18), stem rust (Sr57) and powdery mildew (Pm38) pathogens, and has remained effective in wheat crops for many decades. The partial resistance provi...

journal_title：Plant biotechnology journal

authors： Rinaldo A,Gilbert B,Boni R,Krattinger SG,Singh D,Park RF,Lagudah E,Ayliffe M

更新日期：2017-07-01 00:00:00

abstract：:Cadmium (Cd) is a widespread soil contaminant threatening human health. As an ideal energy plant, sweet sorghum (Sorghum bicolor (L.) Moench) has great potential in phytoremediation of Cd-polluted soils, although the molecular mechanisms are largely unknown. In this study, key factors responsible for differential Cd a...

journal_title：Plant biotechnology journal

authors： Feng J,Jia W,Lv S,Bao H,Miao F,Zhang X,Wang J,Li J,Li D,Zhu C,Li S,Li Y

更新日期：2018-02-01 00:00:00

abstract：:Coordination of multiple transgenes is essential for metabolic engineering of biosynthetic pathways. Here, we report the utilization of two bicistronic systems involving the 2A sequence from the foot-and-mouth disease virus and the internal ribosome entry site (IRES) sequence from the crucifer-infecting tobamovirus to...

journal_title：Plant biotechnology journal

authors： Ha SH,Liang YS,Jung H,Ahn MJ,Suh SC,Kweon SJ,Kim DH,Kim YM,Kim JK

更新日期：2010-10-01 00:00:00

abstract：:In this article, we explore the intellectual property (IP) landscape relevant to the production and commercialization of Carolight(™) , a transgenic multivitamin corn variety created on humanitarian grounds to address micronutrient deficiencies in low-and-middle-income countries. The successful production of this vari...

journal_title：Plant biotechnology journal

authors： Zanga D,Capell T,Zhu C,Christou P,Thangaraj H

更新日期：2016-05-01 00:00:00

abstract：:The advent of genetically modified crops in the late 1980s triggered a regulatory response to the relatively new field of plant genetic engineering. Over a 7-year period, a new regulatory framework was created, based on scientific principles that focused on risk mitigation. The process was transparent and deliberately...

journal_title：Plant biotechnology journal

authors： Smyth S,McHughen A

更新日期：2008-04-01 00:00:00

abstract：:A high-amylose rice with 64.8% amylose content (AC) was developed by transgenic inhibition of two isoforms of starch branching enzyme (SBE), SBEI and SBEIIb, in an indica rice cultivar. The expression of SBEI and SBEIIb was completely inhibited in the transgenic line, whereas the expression of granule-bound starch syn...

journal_title：Plant biotechnology journal

authors： Zhu L,Gu M,Meng X,Cheung SC,Yu H,Huang J,Sun Y,Shi Y,Liu Q

更新日期：2012-04-01 00:00:00

abstract：:In a de novo genotyping-by-sequencing (GBS) analysis of short, 64-base tag-level haplotypes in 4657 accessions of cultivated oat, we discovered 164741 tag-level (TL) genetic variants containing 241224 SNPs. From this, the marker density of an oat consensus map was increased by the addition of more than 70000 loci. The...

journal_title：Plant biotechnology journal

authors： Bekele WA,Wight CP,Chao S,Howarth CJ,Tinker NA

更新日期：2018-08-01 00:00:00

abstract：:Ethylene response factor 1 (ERF1) is an essential integrator of the jasmonate and ethylene signalling pathways coordinating a large number of genes involved in plant defences. Its orthologue in Hevea brasiliensis, HbERF-IXc5, has been assumed to play a major role in laticifer metabolism and tolerance to harvesting str...

journal_title：Plant biotechnology journal

authors： Lestari R,Rio M,Martin F,Leclercq J,Woraathasin N,Roques S,Dessailly F,Clément-Vidal A,Sanier C,Fabre D,Melliti S,Suharsono S,Montoro P

更新日期：2018-01-01 00:00:00

abstract：:A new understanding of leaf starch degradation has emerged in the last 10 years. It has been shown that starch phosphorylation and dephosphorylation are critical components of this process. Glucan, water dikinase (GWD) (and phosphoglucan, water dikinase) adds phosphate to starch, and phosphoglucan phosphatase (SEX4) r...

journal_title：Plant biotechnology journal

authors： Weise SE,Aung K,Jarou ZJ,Mehrshahi P,Li Z,Hardy AC,Carr DJ,Sharkey TD

更新日期：2012-06-01 00:00:00

abstract：:Expression of the cecropin A gene in rice confers resistance to the rice blast fungus Magnaporthe oryzae. In this study, a polymerase chain reaction-based suppression subtractive hybridization approach was used to generate a cDNA macroarray from the elite japonica rice (Oryza sativa L.) cultivar 'Senia'. Gene expressi...

journal_title：Plant biotechnology journal

authors： Campo S,Manrique S,García-Martínez J,San Segundo B

更新日期：2008-08-01 00:00:00