Molecular switch for the assembly of lipophilic drug incorporated plasma protein nanoparticles and in vivo image.

Abstract:

:A strategy to manipulate the disulfide bond breaking triggered unfolding, and subsequently assembly of human serum albumin (HSA) in a lipophilic drug-dependent manner is present. In this study, the hydrophobic region, a molecular switch of the HSA, was regulated to form HSA-paclitaxel (HSA-PTX) nanoparticles by a facile route. High-resolution transmission electron microscopy and fluorescence quenching indicate that HSA coassembled with PTX, which acts as a bridge to form core-shell nanoparticles about 50-240 nm in size, and that PTX might bind to the subdomain IIA sites of HSA. Change of ultraviolet absorption and circular dichroism spectra reveal the formation of HSA-PTX nanoparticles, which is a safety, injectable pharmaceutic nanocarrier system for tumor target. This method to prepare nanocarrier systems for hydrophobic guest molecules reveals a general principle of self-assembly for other plasma proteins and other pharmacologically active substances with poor water solubility. It also provides a basis for developing nanocarrier systems for a wide range of applications in nanomedicine, from drug delivery to bioimaging systems.

journal_name

Biomacromolecules

journal_title

Biomacromolecules

authors

Gong G,Xu Y,Zhou Y,Meng Z,Ren G,Zhao Y,Zhang X,Wu J,Hu Y

doi

10.1021/bm201401s

subject

Has Abstract

pub_date

2012-01-09 00:00:00

pages

23-8

issue

1

eissn

1525-7797

issn

1526-4602

journal_volume

13

pub_type

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