Abstract:
OBJECTIVE:Using genome-wide promoter methylation assay, B cell CLL/lymphoma 6 member B (BCL6B) was found to be preferentially methylated in cancer. A study was undertaken to examine the epigenetic regulation, biological function and clinical significance of BCL6B in gastric cancer (GC). METHODS:BCL6B promoter methylation was evaluated by combined bisulfite restriction analysis and sequencing. The biological functions of BCL6B were determined by cell viability, colony formation, flow cytometry and in vivo tumorigenicity assays. The molecular targets of BCL6B were identified by cDNA expression array. RESULTS:BCL6B was silenced or downregulated in all nine GC cell lines and readily expressed in normal gastric tissues. Loss of BCL6B expression was regulated by promoter hypermethylation. Re-expression of BCL6B in GC cell lines inhibited colony formation, suppressed cell viability, induced apoptosis and restrained the tumorigenecity in nude mice. These effects were associated with upregulation of the pro-apoptosis genes tumour necrosis factor receptor superfamily member 1A, caspase-8, caspase-9, caspase-3 and caspase-7 and nuclear enzyme poly (ADP-ribose) polymerase, downregulation of the pro-proliferation genes S100 calcium binding protein A4 and vascular endothelial growth factor A, and induction of the tumour suppressor genes ataxia telangiectasia mutated homologue and p53. BCL6B hypermethylation was detected in 49.0% (102/208) and 66.3% (67/101) of two independent cohorts of patients with GC, respectively. BCL6B methylation was an independent factor for the survival of patients with GC (p=0.001 for cohort I, p=0.02 for cohort II). CONCLUSIONS:BCL6B plays a pivotal role as a potential tumour suppressor in GC. Detection of methylated BCL6B may serve as an independent biomarker for the prognosis of GC.
journal_name
Gutjournal_title
Gutauthors
Xu L,Li X,Chu ES,Zhao G,Go MY,Tao Q,Jin H,Zeng Z,Sung JJ,Yu Jdoi
10.1136/gutjnl-2011-300411subject
Has Abstractpub_date
2012-07-01 00:00:00pages
977-85issue
7eissn
0017-5749issn
1468-3288pii
gutjnl-2011-300411journal_volume
61pub_type
杂志文章相关文献
GUT文献大全abstract::The plasma clearance and the liver uptake of intravenously administered (64)Cu were significantly impaired in four patients with Wilson's disease. These defects were unlikely to be simply expressions of the high liver copper concentration as the plasma clearance and hepatic uptake of (64)Cu were normal in four patient...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.12.2.139
更新日期:1971-02-01 00:00:00
abstract:OBJECTIVE:Chronic pancreatitis is a progressive inflammatory disorder of the pancreas characterised by permanent destruction of acinar cells. Mutations in the chymotrypsinogen C (CTRC) gene have been linked to the development of chronic pancreatitis. The aim of the present study was to explore whether CTRC mutants indu...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.2009.198903
更新日期:2010-03-01 00:00:00
abstract:BACKGROUND AND AIMS:Little is known about the epidemiology of malignant digestive endocrine tumours. The aim of this study was to report on their incidence and management in a well defined population. METHODS:Data were obtained from the population based Digestive Cancer Registry of Burgundy (France) over a 24 year per...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.2003.026401
更新日期:2004-04-01 00:00:00
abstract:BACKGROUND:Increased expression of proinflammatory cytokines, including tumour necrosis factor alpha, interleukin 6, and interferon gamma, as well as activation of proinflammatory signalling molecules such as nuclear factor kappa B, is characteristic of inflammatory bowel disease (IBD). AIMS:To investigate expression ...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.51.3.379
更新日期:2002-09-01 00:00:00
abstract::Bowel function was assessed in 51 subjects: 10 women and seven men who habitually consumed an omnivorous, vegetarian, or vegan diet. The subjects on these diets had a mean intake of fibre of 23 g, 37 g, and 47 g respectively. Mean transit times were variable and not significantly different between the groups. Vegans, ...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.27.2.164
更新日期:1986-02-01 00:00:00
abstract::This experimental study was undertaken to follow the influence of phenobarbital on bile chemistry and gallstone formation. Phenobarbital (400 mg/kg/day) was administered to golden hamsters receiving a diet known to induce cholesterol gallstones. After a 28-day period none of the control animals had gallstones whereas ...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.14.12.956
更新日期:1973-12-01 00:00:00
abstract::Oral disodium cromoglycate (200 mg qds) has been tested in 26 patients with ulcerative colitis that was resistant to medical treatment. In a double-blind crossover trial disodium cromoglycate and placebo were added to conventional treatment in random order, each for four weeks. There was no significant difference in t...
journal_title:Gut
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1136/gut.19.12.1140
更新日期:1978-12-01 00:00:00
abstract:BACKGROUND:5-Lipoxygenase products play a part in inflammatory response. AIMS:The effect of intracolonic administration of zileuton (a 5-lipoxygenase inhibitor) on colonic damage and eicosanoid local release was assessed in a rat model of colitis. METHODS:Ninety rats with trinitrobenzenesulphonic acid induced colitis...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.38.6.899
更新日期:1996-06-01 00:00:00
abstract::The case of a 44-year-old woman is reported. The diagnosis after the appropriate tests and laparotomy was ACTH-secreting 'apudoma' of the gallbladder. This is a rare tumour and this case is believed to be the first reported of an ectopic hormone producing tumour from this side. ...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.16.6.473
更新日期:1975-06-01 00:00:00
abstract:BACKGROUND:Saccharomyces boulardii is a non-pathogenic yeast which exerts trophic effects on human and rat small intestinal mucosa. AIMS:To examine the effects of S boulardii on ileal adaptation after proximal enterectomy in rats. METHODS:Wistar rats, aged eight weeks, underwent 60% proximal resection or transection ...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.45.1.89
更新日期:1999-07-01 00:00:00
abstract:OBJECTIVE:Pancreatic acinar cell maturation is dependent on the activity of the pancreas transcription factor 1 (PTF1) complex. Induction of pancreatitis leads to MAP kinase activation and transient suppression of the acinar differentiation programme. We investigated the role of MAP kinase-interacting kinase 1 (Mnk1) i...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gutjnl-2013-306068
更新日期:2015-06-01 00:00:00
abstract::In a study of low dose interferon alfa-2b in 587 patients expected to show a good response to treatment, 76-87% patients in different countries had alanine amino-transferase activities returned to normal after four months' treatment, 49-72% were negative for hepatitis B virus (HBV)-DNA, 51-66% were negative for hepati...
journal_title:Gut
pub_type: 临床试验,杂志文章,多中心研究
doi:10.1136/gut.34.2_suppl.s99
更新日期:1993-01-01 00:00:00
abstract:BACKGROUND:Hypersensitivity of gastric afferent pathways may play an aetiological role in symptoms of functional dyspepsia. AIMS:To determine whether patients with severe organic dyspepsia (associated with tissue irritation/injury) and those with functional dyspepsia (no detectable tissue irritation) differ in their p...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.42.6.814
更新日期:1998-06-01 00:00:00
abstract:BACKGROUND:Portal systemic encephalopathy (PSE) is a complex neuropsychiatric syndrome associated with hepatic failure. Small scale studies have shown the benzodiazepine receptor antagonist flumazenil to be effective in ameliorating PSE. AIMS:To determine the efficacy of flumazenil in patients with non-comatous mild t...
journal_title:Gut
pub_type: 临床试验,杂志文章,多中心研究,随机对照试验
doi:10.1136/gut.39.2.319
更新日期:1996-08-01 00:00:00
abstract::Cirrhosis is associated with the development of a hyperdynamic circulation, which is secondary to the presence of systemic vasodilatation. Several mechanisms have been postulated to be involved in the development of systemic vasodilatation, including increased synthesis of nitric oxide, hyperglucagonaemia, increased c...
journal_title:Gut
pub_type: 杂志文章,评审
doi:10.1136/gut.2004.056556
更新日期:2005-12-01 00:00:00
abstract::Duodenal function was studied in 11 healthy volunteers after intragastric instillation of a mixed semi-elemental meal. The duodenum accepted chyme of varying pH, osmolality, and nutrient concentration; and, as a result of biliary, pancreatic, and enteric secretion as well as absorption, it delivered chyme with nearly ...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.19.8.699
更新日期:1978-08-01 00:00:00
abstract::Fasting and postprandial plasma concentrations of glycine and taurine conjugates of cholic, chenodeoxycholic, and deoxycholic acid were measured by a high pressure liquid chromatography-enzymatic assay in nine healthy subjects. The mean value of each bile acid concentration increased significantly (2.4-4.7 times) in t...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.24.3.249
更新日期:1983-03-01 00:00:00
abstract::The behaviour of basal and stimulated acid secretion, gastrin release, serum pepsinogen I, and gastric emptying of liquids was studied in 19 consecutive patients with Helicobacter pylori positive duodenal ulcer, over a follow up period of six months. Eleven patients were studied before and at three and six months afte...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.37.2.210
更新日期:1995-08-01 00:00:00
abstract:BACKGROUND AND AIMS:Coeliac disease (CD) is triggered by an abnormal reaction to gluten. Peptides resulting from partially digested gluten of wheat, barley or rye cause inflammation of the small intestinal mucosa. Previous contradictory studies suggest that oats may trigger the abnormal immunological response in patien...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.2010.225268
更新日期:2011-07-01 00:00:00
abstract::Bacterial contamination of gastrointestinal fibre-endoscopes is a potential source of clinically significant infection. Aqueous 2% alkaline glutaraldehyde adequately disinfects fibre-endoscopes but may cause serious sensitivity reactions among endoscopy staff. A new 'closed-system' disinfecting apparatus is described ...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.23.8.706
更新日期:1982-08-01 00:00:00
abstract::The effects of pentagastrin and the putative gastrin antagonist proglumide on interdigestive motility of the upper small bowel were studied in a randomised double blind study in 10 healthy human volunteers. Intraluminal pressures were recorded manometrically in the duodenum and jejunum for five hours. Sixty minutes af...
journal_title:Gut
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1136/gut.25.9.953
更新日期:1984-09-01 00:00:00
abstract:OBJECTIVE:Liver fibrosis and cirrhosis resulting from chronic liver injury represent a major healthcare burden worldwide. Growth differentiation factor (GDF) 11 has been recently investigated for its role in rejuvenation of ageing organs, but its role in chronic liver diseases has remained unknown. Here, we investigate...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gutjnl-2019-318812
更新日期:2020-06-01 00:00:00
abstract::Diverticulitis affects the right side of the colon more commonly than the left side in the Far East. A consecutive series of 35 patients (mean age 44 years) with right sided diverticulitis seen at this hospital is presented. All had localised pain in the right iliac fossa but systemic upset was uncommon. All but one w...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.33.4.547
更新日期:1992-04-01 00:00:00
abstract:BACKGROUND:Invasion of the intestinal mucosa by leucocytes is a characteristic of intestinal inflammation but the role of the epithelium in orchestrating this recruitment has not been examined in vivo. Cultured intestinal epithelial cells secrete a wide variety of chemokines, often in response to agents present in the ...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.49.4.526
更新日期:2001-10-01 00:00:00
abstract:BACKGROUND:The phenotypic spectrum of familial adenomatous polyposis (FAP) varies from the classic appearance of hundreds of adenomatous colonic polyps in the young adult and early onset colorectal cancer, to the occurrence of sparse adenomas in the older adult, "attenuated" FAP, due to mutations at the 5' or 3' ends o...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.45.6.829
更新日期:1999-12-01 00:00:00
abstract::A single oral dose of 4-46 mmol calcium gluconate at pH 5-6 was administered intragastrically to 15 male volunteers without gastrointestinal disease. There was a significant rise in acid output from 30-90 minutes after the calcium was given compared with the basal hourly collection. The serum gastrin level 30 minutes ...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.18.2.111
更新日期:1977-02-01 00:00:00
abstract:BACKGROUND AND AIMS:Several cases of acute colitis induced by non-steroidal anti-inflammatory drugs (NSAIDs) have been reported but the general role of recent NSAID intake as a risk factor for acute diarrhoea has not been studied to date. The aim of our study was to determine whether the risk of acute diarrhoea is incr...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.52.2.260
更新日期:2003-02-01 00:00:00
abstract::Recent reports have indicated a possible role for 111Indium leucocyte scintigraphy in the management of patients with acute inflammatory bowel disease. Our experience with this technique in 15 patients (nine with ulcerative colitis, six with Crohn's disease) is described. Perfect agreement was not obtained between sci...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.25.12.1372
更新日期:1984-12-01 00:00:00
abstract::The variability in the orocaecal transit time as measured by the lactulose/breath hydrogen method has been studied for three conditions: lactulose given with a meal, subjects sitting; lactulose given with a meal, subjects semirecumbent; lactulose given in aqueous solution, subjects semirecumbent. Thirty three healthy ...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.30.2.171
更新日期:1989-02-01 00:00:00
abstract::Lactulose H2 breath tests are widely used for quantifying carbohydrate malabsorption, but the validity of the commonly used technique (interval sampling of H2 concentrations) has not been systematically investigated. In eight healthy adults we studied the reproducibility of the technique and the accuracy with which 5 ...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.31.1.37
更新日期:1990-01-01 00:00:00