Maps of protein structure space reveal a fundamental relationship between protein structure and function.

abstract：:Methods are described for the removal of the sporophytic pollen grain coating of Brassica oleracea and for the isolation of coat polypeptides. The coat contains a small number of proteins ranging from 6 to 45 kDa. Many of the larger proteins are glycosylated, while all carry high positive charges resulting in pI value...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Doughty J,Hedderson F,McCubbin A,Dickinson H

更新日期：1993-01-15 00:00:00

abstract：:MicroRNAs (miRNAs) are small (approximately 21 nt), noncoding RNAs that negatively regulate target mRNAs at the posttranscriptional level that are involved in development. In plants, virus-induced disease symptoms often result in developmental abnormalities resembling perturbation of miRNA-mediated function. Here, we ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chellappan P,Vanitharani R,Fauquet CM

更新日期：2005-07-19 00:00:00

abstract：:A major barrier to the design of immunotherapeutics and vaccines for cancer is the idiosyncratic nature of many tumor antigens and the possibility that T cells may be tolerant of broadly distributed antigens. We have devised an experimental strategy that exploits species differences in protein sequences to circumvent ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Theobald M,Biggs J,Dittmer D,Levine AJ,Sherman LA

更新日期：1995-12-19 00:00:00

abstract：:A two-dimensional model of the expansion of a crack in an elastic medium is considered in which friction depends on the slip rate and the modulus of cohesion depends on the speed of expansion of the crack. Elastic waves are neglected (quasi-static model). Under some conditions, the expansion of the crack is realized b...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Barenblatt GI,Keilis-Borok VI,Vishik MM

更新日期：1981-09-01 00:00:00

abstract：:In previous work we identified a basilar papilla protein (BPP23) that appears to be one of the most abundant soluble proteins in the basilar papilla of the chick cochlea. Here we report the purification of protein BPP23 from chick cochlea and the generation of a specific antiserum. Immunoblotting and immunoprecipitati...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Oberholtzer JC,Buettger C,Summers MC,Matschinsky FM

更新日期：1988-05-01 00:00:00

abstract：:In vitro and in vivo triplet state electron paramagnetic resonance (epr) spectra of bacteriochlorophylls (Bchls) show important differences in (a) electron spin polarization (esp), and (b) zero field splitting (ZFS) parameters. The unusual esp and ZFS properties of the observed in vivo triplet state are best interpret...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Thurnauer MC,Katz JJ,Norris JR

更新日期：1975-09-01 00:00:00

abstract：:DNA topoisomerase I (top1) is the target of potent anticancer agents, including camptothecins and DNA intercalators, which reversibly stabilize (trap) top1 catalytic intermediates (cleavage complexes). The aim of the present study was to define the structural relationship between the site(s) of covalently bound interc...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pommier Y,Laco GS,Kohlhagen G,Sayer JM,Kroth H,Jerina DM

更新日期：2000-09-26 00:00:00

abstract：:Mechanical stability in many biological materials is provided by the crosslinking of large structural proteins with gamma-glutamyl-epsilon-lysyl amide bonds. The three-dimensional structure of human recombinant factor XIII (EC 2.3.2.13 zymogen; protein-glutamine:amine gamma-glutamyltransferase a chain), a transglutami...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yee VC,Pedersen LC,Le Trong I,Bishop PD,Stenkamp RE,Teller DC

更新日期：1994-07-19 00:00:00

abstract：:Light chain allotypic determinants were demonstrated on the surface of peripheral blood lymphocytes of rabbits. By means of a combined immunofluorescent and autoradiographic technique, a great majority of the individual lymphocytes of rabbits heteroxygous at the b locus were shown to have detectable quantities of only...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Davie JM,Paul WE,Mage RG,Goldman MB

更新日期：1971-02-01 00:00:00

abstract：:Certain complex phenotypes appear repeatedly across diverse species due to processes of evolutionary conservation and convergence. In some contexts like developmental body patterning, there is increased appreciation that common molecular mechanisms underlie common phenotypes; these molecular mechanisms include highly ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rittschof CC,Bukhari SA,Sloofman LG,Troy JM,Caetano-Anollés D,Cash-Ahmed A,Kent M,Lu X,Sanogo YO,Weisner PA,Zhang H,Bell AM,Ma J,Sinha S,Robinson GE,Stubbs L

更新日期：2014-12-16 00:00:00

abstract：:Infection of newborn rats with Borne disease virus (BDV) results in selective degeneration of granule cell neurons of the dentate gyrus (DG). To study cellular countermechanisms that might prevent this pathology, we screened for rat strains resistant to this BDV-induced neuronal degeneration. To this end, we infected ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wu YJ,Schulz H,Lin CC,Saar K,Patone G,Fischer H,Hübner N,Heimrich B,Schwemmle M

更新日期：2013-01-29 00:00:00

abstract：:Receptor-binding specificity of HA, the major surface glycoprotein of influenza virus, primarily determines the host ranges that the virus can infect. Influenza type B virus almost exclusively infects humans and contributes to the annual "flu" sickness. Here we report the structures of influenza B virus HA in complex ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wang Q,Tian X,Chen X,Ma J

更新日期：2007-10-23 00:00:00

abstract：:Bacteriophage display of antibodies provides a method for the generation of immunological reagents against rare and uncharacterized antigens. To ascertain the usefulness of this approach for the characterization of inner-ear proteins, we produced a bacteriophage-displayed antibody-fragment library directed against pro...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cyr JL,Hudspeth AJ

更新日期：2000-02-29 00:00:00

abstract：:Influenza viruses contain segmented, negative-strand RNA genomes. Genome segmentation facilitates reassortment between different influenza virus strains infecting the same cell. This phenomenon results in the rapid exchange of RNA segments. In this study, we have developed a method to prevent the free reassortment of ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gao Q,Palese P

更新日期：2009-09-15 00:00:00

abstract：:Malignant myeloid leukemic cells and normal macrophages and granulocytes have functional beta-adrenergic receptors, which have been quantitated by radioreceptor binding with the beta-adrenergic antagonist [(3)H]dihydroalprenolol and by induction of cyclic AMP by adrenergic hormones. Both the normal and leukemic cells ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Simantov R,Sachs L

更新日期：1978-04-01 00:00:00

abstract：:The "false thumb" of pandas is a carpal bone, the radial sesamoid, which has been enlarged and functions as an opposable thumb. If the giant panda (Ailuropoda melanoleuca) and the red panda (Ailurus fulgens) are not closely related, their sharing of this adaptation implies a remarkable convergence. The discovery of pr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Salesa MJ,Antón M,Peigné S,Morales J

更新日期：2006-01-10 00:00:00

abstract：:Opitz syndrome (OS) is a genetically heterogeneous disorder characterized by defects of the ventral midline, including hypertelorism, cleft lip and palate, heart defects, and mental retardation. We recently identified the gene responsible for X-linked OS. The ubiquitously expressed gene product, MID1, is a member of t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schweiger S,Foerster J,Lehmann T,Suckow V,Muller YA,Walter G,Davies T,Porter H,van Bokhoven H,Lunt PW,Traub P,Ropers HH

更新日期：1999-03-16 00:00:00

abstract：:Isolated human erythrocyte spectrin is a dimer of two unique polypeptide chains. The dimer (alpha beta) undergoes reversible salt- and temperature-dependent association to form (alpha beta)2 tetramers. Spectrin also binds with high affinity to a protein receptor on the cytoplasmic surface of erythrocyte membrane vesic...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Morrow JS,Speicher DW,Knowles WJ,Hsu CJ,Marchesi VT

更新日期：1980-11-01 00:00:00

abstract：:Whereas the laws of thermodynamics prohibit extraction of useful work from the Brownian motion of particles in equilibrium, these motions can be "rectified" under nonequilibrium conditions, for example, in the presence of asymmetric geometrical obstacles. Here, we describe a class of systems in which aerobic bacteria ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sokolov A,Apodaca MM,Grzybowski BA,Aranson IS

更新日期：2010-01-19 00:00:00

abstract：:Anti-idiotype antibodies (Ab2) were purified from a cancer patient treated with NP-4, a murine monoclonal antibody to carcinoembryonic antigen (CEA). These Ab2 were specific for NP-4 and inhibited the binding between NP-4 and CEA. BALB/c mice immunized with these human Ab2 produced anti-Ab2 antibodies that were also r...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Losman MJ,Hansen HJ,Sharkey RM,Goldenberg DM,Monestier M

更新日期：1991-04-15 00:00:00

abstract：:At meiotic prophase, chromatin loops around a proteinaceous core, with the sizes of these loops varying between species. Comparison of the morphology of sequence-related inserts at different sites in transgenic mice demonstrates that loop size also varies with chromosomal geography. Similarly, chromatin loop lengths d...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Heng HH,Chamberlain JW,Shi XM,Spyropoulos B,Tsui LC,Moens PB

更新日期：1996-04-02 00:00:00

abstract：:CCAAT/enhancer binding protein (C/EBP) epsilon is a recently cloned member of the C/EBP family of transcription factors and is expressed exclusively in cells of hematopoietic origin. The human C/EBPepsilon gene is transcribed by two alternative promoters, Palpha and Pbeta. A combination of differential splicing and al...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yamanaka R,Kim GD,Radomska HS,Lekstrom-Himes J,Smith LT,Antonson P,Tenen DG,Xanthopoulos KG

更新日期：1997-06-10 00:00:00

abstract：:The product of the Escherichia coli secB gene is required for efficient export of proteins across the cytoplasmic membrane. The studies described in this report show that in wild-type growing cells, SecB protein associates with precursor forms of exported proteins, such as the periplasmic maltose-binding protein (MBP)...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kumamoto CA

更新日期：1989-07-01 00:00:00

abstract：:Remarkable progress has come from whole-brain models linking anatomy and function. Paradoxically, it is not clear how a neuronal dynamical system running in the fixed human anatomical connectome can give rise to the rich changes in the functional repertoire associated with human brain function, which is impossible to ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kringelbach ML,Cruzat J,Cabral J,Knudsen GM,Carhart-Harris R,Whybrow PC,Logothetis NK,Deco G

更新日期：2020-04-28 00:00:00

abstract：:The breast and ovarian cancer suppressor BRCA2 controls the enzyme RAD51 during homologous DNA recombination (HDR) to preserve genome stability. BRCA2 binds to RAD51 through 8 conserved BRC repeat motifs dispersed in an 1127-residue region (BRCA2([BRC1-8])). Here, we show that BRCA2([BRC1-8]) exerts opposing effects o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shivji MK,Mukund SR,Rajendra E,Chen S,Short JM,Savill J,Klenerman D,Venkitaraman AR

更新日期：2009-08-11 00:00:00

abstract：:2(S)-dihydroxypropanesulfonate (DHPS) is a microbial degradation product of 6-deoxy-6-sulfo-d-glucopyranose (sulfoquinovose), a component of plant sulfolipid with an estimated annual production of 1010 tons. DHPS is also at millimolar levels in highly abundant marine phytoplankton. Its degradation and sulfur recycling...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Liu J,Wei Y,Lin L,Teng L,Yin J,Lu Q,Chen J,Zheng Y,Li Y,Xu R,Zhai W,Liu Y,Liu Y,Cao P,Ang EL,Zhao H,Yuchi Z,Zhang Y

更新日期：2020-07-07 00:00:00

abstract：:No microorganism capable of anaerobic growth on methane as the sole carbon source has yet been cultivated. Consequently, information about these microbes has been inferred from geochemical and microbiological observations of field samples. Stable isotope analysis of lipid biomarkers and rRNA gene surveys have implicat...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Orphan VJ,House CH,Hinrichs KU,McKeegan KD,DeLong EF

更新日期：2002-05-28 00:00:00

abstract：:NADH:quinone oxidoreductase (complex I) plays a pivotal role in cellular energy production. It employs a series of redox cofactors to couple electron transfer to the generation of a proton-motive force across the inner mitochondrial or bacterial cytoplasmic membrane. Complex I contains a noncovalently bound flavin mon...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yakovlev G,Reda T,Hirst J

更新日期：2007-07-31 00:00:00

abstract：:We show that the stimulation of human immunodeficiency virus (HIV) brought about by tumor necrosis factor alpha and phorbol 12-myristate 13-acetate can be inhibited by adding N-acetyl-L-cysteine (NAC). NAC, which replenishes intracellular glutathione, effectively inhibits the tumor necrosis factor alpha- or phorbol es...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Roederer M,Staal FJ,Raju PA,Ela SW,Herzenberg LA,Herzenberg LA

更新日期：1990-06-01 00:00:00

abstract：:AIDS remains incurable due to the permanent integration of HIV-1 into the host genome, imparting risk of viral reactivation even after antiretroviral therapy. New strategies are needed to ablate the viral genome from latently infected cells, because current methods are too inefficient and prone to adverse off-target e...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hu W,Kaminski R,Yang F,Zhang Y,Cosentino L,Li F,Luo B,Alvarez-Carbonell D,Garcia-Mesa Y,Karn J,Mo X,Khalili K

更新日期：2014-08-05 00:00:00