Abstract:
:Knowledge of the structure of a viroid is critically important to elucidate the roles played by the various RNA motifs in the steps of the viroid's life cycle. A new technique, RNA-selective 2'-hydroxyl acylation analysed by primer extension (SHAPE), has recently been shown to be fast, reliable and applicable to the study of various RNA molecules. Consequently, this method was used to probe sequence variants of Peach latent mosaic viroid (PLMVd). Initially, probing data from RNA strands of both polarities of the Siberian C variant confirmed the secondary structures previously determined using both conventional and fastidious approaches. Subsequently, analysis of an Alberta variant showed an identical structure for the strand of (-) polarity, but the (+) polarity strand exhibited two differences from the Siberian C variant: the P11-L11 stem-loop domain formed a cruciform structure, and nucleotides from loops L1 and L11 were involved in the formation of a pseudoknot. The existence of both of these motifs was confirmed by site-directed mutagenesis. The subsequent probing of 12 natural sequence variants led to the elucidation of the criteria governing the formation of this novel pseudoknot. Importantly, this study revealed that the heterogeneity of a viroid is not limited to its nucleotide sequence, but may also occur at the structural level.
journal_name
Mol Plant Patholjournal_title
Molecular plant pathologyauthors
Dubé A,Bolduc F,Bisaillon M,Perreault JPdoi
10.1111/j.1364-3703.2010.00703.xsubject
Has Abstractpub_date
2011-09-01 00:00:00pages
688-701issue
7eissn
1464-6722issn
1364-3703journal_volume
12pub_type
杂志文章abstract::Homologous recombination is required for gene-targeted procedures such as gene disruption and gene replacement. Ku80 is part of the non-homologous end-joining DNA repair mechanism in many organisms. We identified and disrupted the Ku80 homologue in Sclerotinia sclerotiorum and generated heterokaryon mutants enriched w...
journal_title:Molecular plant pathology
pub_type: 杂志文章
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更新日期:2008-09-01 00:00:00
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journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12577
更新日期:2018-04-01 00:00:00
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journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12924
更新日期:2020-05-01 00:00:00
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journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12856
更新日期:2019-11-01 00:00:00
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journal_title:Molecular plant pathology
pub_type: 杂志文章
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更新日期:2011-05-01 00:00:00
abstract::Two tobacco vein necrosis (TVN) determinants, the residues K(400) and E(419) , have been identified previously in the helper component-protease (HC-Pro) protein sequence of Potato virus Y (PVY). However, since their description, non-necrotic PVY isolates with both K(400) and E(419) necrotic determinants have been repo...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/j.1364-3703.2012.00803.x
更新日期:2012-10-01 00:00:00
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journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/j.1364-3703.2005.00274.x
更新日期:2005-03-01 00:00:00
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journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12374
更新日期:2017-01-01 00:00:00
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journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12644
更新日期:2018-07-01 00:00:00
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journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12485
更新日期:2017-12-01 00:00:00
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journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1046/j.1364-3703.2003.00161.x
更新日期:2003-05-01 00:00:00
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journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1046/j.1364-3703.2002.00134.x
更新日期:2002-11-01 00:00:00
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journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1046/j.1364-3703.2000.00007.x
更新日期:2000-01-01 00:00:00
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journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12819
更新日期:2019-08-01 00:00:00
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journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1046/j.1364-3703.2001.00059.x
更新日期:2001-05-01 00:00:00
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journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/j.1364-3703.2009.00546.x
更新日期:2009-07-01 00:00:00
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journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/j.1364-3703.2009.00537.x
更新日期:2009-05-01 00:00:00
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journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1046/j.1364-3703.2003.00138.x
更新日期:2003-01-01 00:00:00
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journal_title:Molecular plant pathology
pub_type: 杂志文章,评审
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更新日期:2012-06-01 00:00:00
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journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12114
更新日期:2014-08-01 00:00:00
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journal_title:Molecular plant pathology
pub_type: 杂志文章
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更新日期:2011-01-01 00:00:00
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journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/j.1364-3703.2008.00525.x
更新日期:2009-03-01 00:00:00
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journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12214
更新日期:2015-08-01 00:00:00
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journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12899
更新日期:2020-03-01 00:00:00
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journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1046/j.1364-3703.2000.00028.x
更新日期:2000-07-01 00:00:00
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journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/j.1364-3703.2007.00385.x
更新日期:2007-03-01 00:00:00
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journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12104
更新日期:2014-06-01 00:00:00
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journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12134
更新日期:2014-09-01 00:00:00
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journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/j.1364-3703.2012.00842.x
更新日期:2013-02-01 00:00:00
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journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/j.1364-3703.2007.00434.x
更新日期:2007-11-01 00:00:00