Genome structure-based screening identified epigenetically silenced microRNA associated with invasiveness in non-small-cell lung cancer.


:MicroRNA (miRNA) expression is frequently altered in human cancers. To search for epigenetically silenced miRNAs in non-small-cell lung cancer (NSCLC), we mapped human miRNAs on autosomal chromosomes and selected 55 miRNAs in silico. We treated six NSCLC cell lines with the DNA methylation inhibitor 5-aza-2'-deoxycytidine (5-aza-CdR) and determined the expressions of the 55 miRNAs. Fourteen miRNAs were decreased in the cancer cell lines and were induced after 5-aza-CdR treatment. After a detailed DNA methylation analysis, we found that mir-34b and mir-126 were silenced by DNA methylation. Mir-34b was silenced by the DNA methylation of its own promoter, whereas mir-126 was silenced by the DNA methylation of its host gene, EGFL7. A chromatin immunoprecipitation assay revealed H3K9me2 and H3K9me3 in mir-34b and EGFL7, and H3K27me3 in EGFL7. The overexpression of mir-34b and mir-126 decreased the expression of c-Met and Crk, respectively. The 5-aza-CdR treatment of lung cancer cell line resulted in increased mir-34b expression and decreased c-Met protein. We next analyzed the DNA methylation status of these miRNAs using 99 primary NSCLCs. Mir-34b and mir-126 were methylated in 41 and 7% of all the cases, respectively. The DNA methylation of mir-34b was not associated with c-Met expression determined by immunohistochemistry, but both mir-34b methylation (p = 0.007) and c-Met expression (p = 0.005) were significantly associated with lymphatic invasion in a multivariate analysis. The DNA methylation of mir-34b can be used as a biomarker for an invasive phenotype of lung cancer.


Int J Cancer


Watanabe K,Emoto N,Hamano E,Sunohara M,Kawakami M,Kage H,Kitano K,Nakajima J,Goto A,Fukayama M,Nagase T,Yatomi Y,Ohishi N,Takai D




Has Abstract


2012-06-01 00:00:00












  • Inhibition of growth of certain human tumour cell lines by a factor derived from human fibroblast-like cell lines. I. Demonstration by mixed culture and by use of cell washings.

    abstract::The growth of 7/9 Burkitt's lymphoma (BL) cell lines and 5/6 human melanoma cell lines was inhibited by certain human fibroblast-like cell lines, especially human myofibroblast cell lines. In contrast to BL lines, 3/5 lymphoblastoid cell lines were less susceptible and 3/4 human leukaemic lines were resistant. The eff...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Wu KF,Pope JH,Ellem KA

    更新日期:1985-04-15 00:00:00

  • Parity and breast cancer risk: possible effect on age at diagnosis.

    abstract::We examined the effect of parity on breast cancer risk on the basis of information from 107,146 married female nurses followed prospectively between 1976-1980. For everparous women, a significant protective effect against breast cancer was observed with increasing parity (chi 12 for trend = 14.2, p less than 0.001). A...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Pathak DR,Speizer FE,Willett WC,Rosner B,Lipnick RJ

    更新日期:1986-01-15 00:00:00

  • Inhibitory effect of 4-methylesculetin on hyaluronan synthesis slows the development of human pancreatic cancer in vitro and in nude mice.

    abstract::We report the inhibitory effect of 4-methylesculetin (ME), a 4-methylumbelliferone derivative, on hyaluronan (HA) synthesis by pancreatic cancer cells, and its resulting anticancer action. First, HA in cell culture was analyzed using competitive inhibition with hyaluronic acid-binding protein (HABP) to study HA synthe...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Hajime M,Shuichi Y,Makoto N,Masanori Y,Ikuko K,Atsushi K,Mutsuo S,Keiichi T

    更新日期:2007-06-15 00:00:00

  • Overexpression of the met/HGF receptor in renal cell carcinomas.

    abstract::The c-met oncogene encodes the receptor for hepatocyte growth factor/scatter factor (HGF/SF), a multifunctional cytokine able to mediate morphogenesis as well as mitogenesis, motogenesis and invasiveness of epithelial cells. HGF/SF has been implicated in branching tubulogenesis of the developing kidney and in regenera...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Natali PG,Prat M,Nicotra MR,Bigotti A,Olivero M,Comoglio PM,Di Renzo MF

    更新日期:1996-06-21 00:00:00

  • Novel senescence associated gene, YPEL3, is repressed by estrogen in ER+ mammary tumor cells and required for tamoxifen-induced cellular senescence.

    abstract::Estrogen signaling plays an important role in breast carcinogenesis. An increased understanding of estrogen gene targets and their effects will allow for more directed and effective therapies for individuals with breast cancer, particularly those with estrogen receptor positive tumors resistant to tamoxifen therapy. H...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Tuttle R,Miller KR,Maiorano JN,Termuhlen PM,Gao Y,Berberich SJ

    更新日期:2012-05-15 00:00:00

  • Epstein-Barr virus-specific IgA serum antibodies as an outstanding feature of nasopharyngeal carcinoma.

    abstract::Stimulated by a report on elevated IgA levels in nasopharyngeal carcinoma (NPC), we tested a total of 372 sera from patients with NPC, other carcinomas of head and neck or elsewhere, Burkitt's lymphoma (BL), infectious mononucleosis (IM) or healthy controls. The sera were titrated in indirect immunofluorescence tests ...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Henle G,Henle W

    更新日期:1976-01-15 00:00:00

  • Integrated gene copy number and expression microarray analysis of gastric cancer highlights potential target genes.

    abstract::We performed an integrated array comparative genomic hybridization (aCGH) and expression microarray analysis of 8 normal gastric tissues and 38 primary tumors, including 25 intestinal and 13 diffuse gastric adenocarcinomas to identify genes whose expression is deregulated in association with copy number alteration. Ou...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Myllykangas S,Junnila S,Kokkola A,Autio R,Scheinin I,Kiviluoto T,Karjalainen-Lindsberg ML,Hollmén J,Knuutila S,Puolakkainen P,Monni O

    更新日期:2008-08-15 00:00:00

  • CD4+FoxP3+ regulatory T cells gradually accumulate in gliomas during tumor growth and efficiently suppress antiglioma immune responses in vivo.

    abstract::The suppressive activity of regulatory T cells (Treg) has been implicated as an important factor limiting immune mediated destruction of tumor cells. However, not much is known about the presence and function of Treg within tumors. Here we show in a syngeneic murine glioma model a time-dependent accumulation of CD4+Fo...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Grauer OM,Nierkens S,Bennink E,Toonen LW,Boon L,Wesseling P,Sutmuller RP,Adema GJ

    更新日期:2007-07-01 00:00:00

  • A novel high-affinity human monoclonal antibody to mesothelin.

    abstract::Mesothelin is a glycosylphosphatidylinositol-anchored glycoprotein that is highly expressed on the cell surface of mesothelioma, ovarian cancer and other malignant tumors. The interaction between mesothelin and CA125 (also called MUC16) may facilitate the implantation and metastasis of tumors in the peritoneal cavity....

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Ho M,Feng M,Fisher RJ,Rader C,Pastan I

    更新日期:2011-05-01 00:00:00

  • Spatiotemporal homogeneity and distinctness of the T-cell receptor β-chain repertoires in Epstein-Barr virus-associated primary and metastatic nasopharyngeal carcinomas.

    abstract::Nasopharyngeal carcinoma (NPC) is an Epstein-Barr virus (EBV)-associated lymphoepithelioma. The aim of this study was to characterize the homogeneity and distinctness of the T-cell repertoires within and between primary and metastatic NPCs. We used ultra-deep sequencing of the hypervariably rearranged antigen-binding ...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Chung YL,Wu ML

    更新日期:2018-08-01 00:00:00

  • Expression of mucin-associated tumor antigens is altered by cell density.

    abstract::Mucin-associated sialylated Lewis antigens are implicated in tumor cell metastasis and are used in several tests for pancreatic cancer. Despite their clinical importance, little is known about the structures of the oligosaccharides of pancreatic cancer mucins or about the regulation of their synthesis or of the synthe...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Yamashita Y,Ho JJ,Cheng S,Siddiki B,Chung YS,Sowa M,Kim YS

    更新日期:1997-07-29 00:00:00

  • Anal and perianal squamous carcinomas and high-grade intraepithelial lesions exclusively associated with "low-risk" HPV genotypes 6 and 11.

    abstract::Anal squamous cell carcinomas are predominantly associated with high-risk human papillomaviruses (HPVs), particularly HPV 16, similar to cervical, vaginal and vulvar cancers. Although the presence of "low-risk" HPVs, in particular genotypes 6 and 11, have occasionally been reported in various HPV-related anogenital ca...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Cornall AM,Roberts JM,Garland SM,Hillman RJ,Grulich AE,Tabrizi SN

    更新日期:2013-11-01 00:00:00

  • Common germline variation in mismatch repair genes and survival after a diagnosis of colorectal cancer.

    abstract::The mismatch repair (MMR) genes are involved in the maintenance of genomic integrity. Recently, we showed that common variants in these genes are unlikely to contribute significantly to colorectal cancer risk. The aim of this study was to investigate the role of common variants in the mismatch repair pathway as progno...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Koessler T,Azzato EM,Perkins B,Macinnis RJ,Greenberg D,Easton DF,Pharoah PD

    更新日期:2009-04-15 00:00:00

  • Association of two mutations in the CHEK2 gene with breast cancer.

    abstract::The 1100delC mutation of the cell cycle checkpoint kinase 2 (CHEK2) gene confers an increased risk for breast cancer, but the clinical impact of other CHEK2 gene variants remains unclear. We determined the frequency of two functionally relevant CHEK2 gene mutations, I157T and IVS2+1G > A, in two large series of breast...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Bogdanova N,Enssen-Dubrowinskaja N,Feshchenko S,Lazjuk GI,Rogov YI,Dammann O,Bremer M,Karstens JH,Sohn C,Dörk T

    更新日期:2005-08-20 00:00:00

  • Epidemiology of hepatitis viruses among hepatocellular carcinoma cases and healthy people in Egypt: a systematic review and meta-analysis.

    abstract::Liver cancers are strongly linked to hepatitis B virus (HBV) and hepatitis C virus (HCV). Egypt has the highest prevalence of HCV worldwide and has rising rates of hepatocellular carcinoma (HCC). Egypt's unique nature of liver disease presents questions regarding the distribution of HBV and HCV in the etiology of HCC....

    journal_title:International journal of cancer

    pub_type: 杂志文章,meta分析,评审


    authors: Lehman EM,Wilson ML

    更新日期:2009-02-01 00:00:00

  • p53 gene mutations in oropharyngeal carcinomas: a comparison of solitary and multiple primary tumours and lymph-node metastases.

    abstract::Despite the steadily increasing number of patients suffering from squamous-cell carcinomas of the oropharyngeal region, little is known about the molecular steps involved in the induction of these neoplasms. We investigated oropharyngeal cancers from 38 patients for mutations in the p53 tumour-suppressor gene. The maj...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Zariwala M,Schmid S,Pfaltz M,Ohgaki H,Kleihues P,Schäfer R

    更新日期:1994-03-15 00:00:00

  • Pregnancy outcomes in childhood cancer survivors: probable effects of abdominal irradiation.

    abstract::A postal survey addressed to 2,083 general practitioners of childhood cancer survivors of reproductive age has revealed that females having undergone direct abdominal irradiation (exposed), particularly for Wilms' tumour, have an increased risk of several adverse pregnancy outcomes as compared with female survivors of...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Hawkins MM,Smith RA

    更新日期:1989-03-15 00:00:00

  • Preservation of chromosome and DNA characteristics of human colorectal adenocarcinomas after passage in nude mice.

    abstract::Twenty-seven human colorectal adenocarcinomas were implanted s.c. into nude mice. A comparative study of chromosomes and DNA between fresh tumor (FT) and xenografted tumor cells (XT) could be performed in 9 cases. Losses or deletions were consistently found in FTs as well as in XTs, particularly on chromosomes 17 and/...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Lefrançois D,Olschwang S,Delattre O,Muleris M,Dutrillaux AM,Thomas G,Dutrillaux B

    更新日期:1989-11-15 00:00:00

  • Association of HPV35 with cervical carcinogenesis among women of African ancestry: Evidence of viral-host interaction with implications for disease intervention.

    abstract::HPV35 has been found in only ∼2% of invasive cervical cancers (ICC) worldwide but up to 10% in Sub-Saharan Africa, warranting further investigation and consideration of impact on preventive strategies. We studied HPV35 and ethnicity, in relation to the known steps in cervical carcinogenesis, using multiple large epide...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Pinheiro M,Gage JC,Clifford GM,Demarco M,Cheung LC,Chen Z,Yeager M,Cullen M,Boland JF,Chen X,Raine-Bennett T,Steinberg M,Bass S,Befano B,Xiao Y,Tenet V,Walker J,Zuna R,Poitras NE,Gold MA,Dunn T,Yu K,Zhu B,Bu

    更新日期:2020-11-15 00:00:00

  • Suppressing effects of dietary supplementation of soybean trypsin inhibitor on spontaneous, experimental and peritoneal disseminated metastasis in mouse model.

    abstract::The modifying effects of a Kunitz trypsin inhibitor (KTI) and a Bowman-Birk trypsin inhibitor (BBI), purified from soybean trypsin inhibitor, as dietary supplements on experimental and spontaneous pulmonary metastasis of murine Lewis lung carcinoma 3LL cells as well as peritoneal disseminated metastasis model in human...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Kobayashi H,Fukuda Y,Yoshida R,Kanada Y,Nishiyama S,Suzuki M,Kanayama N,Terao T

    更新日期:2004-11-10 00:00:00

  • A distinct DNA methylation profile associated with microsatellite and chromosomal stable sporadic colorectal cancers.

    abstract::Aberrant DNA methylation, microsatellite instability (MSI) and chromosomal instability (CIN) are well-characterised molecular features of sporadic colorectal cancers (CRCs). In addition to CpG island methylator phenotype (CIMP) associated with MSI, an intermediate methylation subgroup is also a feature of non-MSI canc...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Silver A,Sengupta N,Propper D,Wilson P,Hagemann T,Patel A,Parker A,Ghosh A,Feakins R,Dorudi S,Suraweera N

    更新日期:2012-03-01 00:00:00

  • Spontaneous remission of residual post-therapy plasma Epstein-Barr virus DNA and its prognostic implication in nasopharyngeal carcinoma: A large-scale, big-data intelligence platform-based analysis.

    abstract::Detectable post-therapy plasma Epstein-Barr virus (EBV) DNA predicts poor survival in non-metastatic nasopharyngeal carcinoma (NPC). However, some patients subsequently experience spontaneous remission of residual EBV DNA during follow-up and it was unclear whether these patients were still at high risk of disease fai...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Zhang Y,Tang LL,Li YQ,Liu X,Liu Q,Ma J

    更新日期:2019-05-01 00:00:00

  • Renal cell carcinoma does not express argininosuccinate synthetase and is highly sensitive to arginine deprivation via arginine deiminase.

    abstract::Recently, pegylated arginine deiminase (ADI; EC has been used to treat the patients with hepatocellular carcinoma or melanoma, in which the level of argininosuccinate synthetase (ASS) activity is low or undetectable. The efficacy of its antitumor activity largely depends on the level of intracellular ASS, whi...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Yoon CY,Shim YJ,Kim EH,Lee JH,Won NH,Kim JH,Park IS,Yoon DK,Min BH

    更新日期:2007-02-15 00:00:00

  • Reducing overtreatment associated with overdiagnosis in cervical cancer screening-A model-based benefit-harm analysis for Austria.

    abstract::A general concern exists that cervical cancer screening using human papillomavirus (HPV) testing may lead to considerable overtreatment. We evaluated the trade-off between benefits and overtreatment among different screening strategies differing by primary tests (cytology, p16/Ki-67, HPV alone or in combinations), int...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Sroczynski G,Esteban E,Widschwendter A,Oberaigner W,Borena W,von Laer D,Hackl M,Endel G,Siebert U

    更新日期:2020-08-15 00:00:00

  • Human melanoma cells can be killed in vitro by an immunotoxin specific for melanoma-associated antigen p97.

    abstract::Conjugates (immunotoxins) comprising ricin A-chain and monoclonal antibody 96.5, which is specific for human melanoma-associated antigen p97, inhibited protein synthesis and colony formation of cultured human melanoma cells that expressed more than 80,000 molecules of p97 per cell. Cells expressing fewer than 5,000 mo...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Casellas P,Brown JP,Gros O,Gros P,Hellström I,Jansen FK,Poncelet P,Roncucci R,Vidal H,Hellström KE

    更新日期:1982-10-15 00:00:00

  • Radiomics predicts response of individual HER2-amplified colorectal cancer liver metastases in patients treated with HER2-targeted therapy.

    abstract::The aim of our study was to develop and validate a machine learning algorithm to predict response of individual HER2-amplified colorectal cancer liver metastases (lmCRC) undergoing dual HER2-targeted therapy. Twenty-four radiomics features were extracted after 3D manual segmentation of 141 lmCRC on pretreatment portal...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Giannini V,Rosati S,Defeudis A,Balestra G,Vassallo L,Cappello G,Mazzetti S,De Mattia C,Rizzetto F,Torresin A,Sartore-Bianchi A,Siena S,Vanzulli A,Leone F,Zagonel V,Marsoni S,Regge D

    更新日期:2020-12-01 00:00:00

  • Variant estrogen receptor alpha mRNAs in human breast cancer specimens.

    abstract::A panel of human breast cancer specimens was examined for single base change mutations by DNA sequencing and for larger deletions using a PCR-based assay. In the cancer specimens examined, no sequencing variants were detected other than a previously characterized polymorphism. Although most of the specimens contained ...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Anandappa SY,Sibson R,Platt-Higgins A,Winstanley JH,Rudland PS,Barraclough R

    更新日期:2000-10-15 00:00:00

  • Immunodetection of cathepsins B and L present in and secreted from human pre-malignant and malignant colorectal tumour cell lines.

    abstract::Pre-malignant and malignant human colorectal tumour epithelial cell lines both secreted precursor forms of the 2 cysteine proteinases, cathepsins B and L. The amount of proteinases secreted by these cell lines varied according to the cell density. Comparison at similar cell densities showed that the pre-malignant, ade...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Maciewicz RA,Wardale RJ,Etherington DJ,Paraskeva C

    更新日期:1989-03-15 00:00:00

  • Investigating Black-White differences in prostate cancer prognosis: A systematic review and meta-analysis.

    abstract::The case-fatality rate following a diagnosis of prostate cancer is higher for Black men compared to White men. How this elevated rate arises is uncertain, with differences in disease biology, presentation, treatment and comorbidity having been suggested. A systematic search was conducted for articles that reported eth...

    journal_title:International journal of cancer

    pub_type: 杂志文章,meta分析,评审


    authors: Evans S,Metcalfe C,Ibrahim F,Persad R,Ben-Shlomo Y

    更新日期:2008-07-15 00:00:00

  • Plasma DNA microsatellite panel as sensitive and tumor-specific marker in lung cancer patients.

    abstract::The majority of lung cancer patients have tumor-derived genetic alterations in circulating plasma DNA that could be exploited as a diagnostic tool. We used fluorescent microsatellite analysis to detect alterations in plasma and tumor DNA in 34 patients who underwent bronchoscopy for lung cancer, including 11 small cel...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Beau-Faller M,Gaub MP,Schneider A,Ducrocq X,Massard G,Gasser B,Chenard MP,Kessler R,Anker P,Stroun M,Weitzenblum E,Pauli G,Wihlm JM,Quoix E,Oudet P

    更新日期:2003-06-20 00:00:00