Abstract:
:In the past, it has been impossible to carry out immunohistological staining as part of a rapid histopathological diagnosis during surgery because the available methods were too time-consuming. The present report introduces a rapid indirect immunoperoxidase method that requires only 10-12 min. Thus, the immunological characterization of tumour cells may now be applied as an additional parameter for histopathological diagnosis during surgery. In this first attempt, the monoclonal antibody Ki-67, which reacts with a human nuclear antigen present in proliferating cells and absent in quiescent cells, was applied with a new immunostaining method and the results were compared with those obtained by the more time-consuming routine immunohistological method. There was no significant difference between the percentages of Ki-67-positive cells determined with the two methods in normal or neoplastic human tissues. Since knowledge of the growth rate of a malignant tumour may be of prognostic and/or therapeutic value in individual cases, it might be useful to routinely apply this new approach for histopathological diagnosis during surgery.
journal_name
Int J Cancerjournal_title
International journal of cancerauthors
Gerdes Jdoi
10.1002/ijc.2910350205subject
Has Abstractpub_date
1985-02-15 00:00:00pages
169-71issue
2eissn
0020-7136issn
1097-0215journal_volume
35pub_type
杂志文章abstract::An increased risk of prostate cancer associated with a family history of prostate cancer has been documented in multiple published reports. Risk has been shown to vary by degree of relationship and age of onset of disease in the affected relative. Several studies, using various designs, have estimated the relative ris...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.11466
更新日期:2003-12-10 00:00:00
abstract::No animal model exists for the in vivo growth of Hodgkin's-lymphoma-derived cells. Neither unmanipulated Hodgkin's-disease(HD)-derived cell lines nor primary biopsy tissue could be grown in nude mice. Since the severe combined immunodeficient (SCID) mouse has been reported to be a better recipient for transplanted hum...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910520610
更新日期:1992-12-02 00:00:00
abstract::Non-steroidal anti-inflammatory drugs (NSAIDs), including aspirin, seem to have chemopreventive properties against several types of cancer, particularly colon cancer. Persons with rheumatoid arthritis, an autoimmune disease for which NSAIDs are used commonly, have been reported to be at lower risk of colon cancer but ...
journal_title:International journal of cancer
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1002/ijc.11311
更新日期:2003-09-20 00:00:00
abstract::A clone of Cloudman S91 murine melanoma was fused in vitro with non-malignant hamster cheek pouch cells by means of lysolecithin, and the putative hybrid progeny cells, HCP-MM, were found to be highly malignant in hamster, but not in appropriate mice. A malignant clone of HCP-MM cells was shown to have hamster species...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910150214
更新日期:1975-02-15 00:00:00
abstract::Somatic cell hybrids have been made between a transformed mouse bladder carcinoma cell line and normal mouse bladder epithelium and mesenchyme. In the epithelial tumour/mesenchyme hybrids the malignant phenotype was expressed dominantly whereas in the carcinoma/normal epithelium hybrids the malignant potential was gre...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910330517
更新日期:1984-05-15 00:00:00
abstract::More than two-thirds of patients with hepatocellular carcinoma (HCC) cannot receive curative therapy and have poor survival due to late diagnosis and few prognostic directions. In our study, nontargeted and targeted metabolomics analyses were conducted by liquid chromatography-mass spectrometry to characterize metabol...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.32599
更新日期:2020-03-15 00:00:00
abstract::Our objective was to examine whether parental exposure to atomic bomb radiation has led to increased cancer and/or noncancer mortality rates among the offspring. We studied 41,010 subjects born from May 1946 through December 1984 (i.e., conceived between 1 month and 38 years after the bombings) and surviving for at le...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.11400
更新日期:2003-11-01 00:00:00
abstract::Human colon cancer cells produce and secrete a variety of polypeptide growth factors. The functional role of these growth factors, however, is poorly understood. Though the secretion of epidermal growth factor (EGF)-like activity and EGF-related molecules by human colon cancer cells in culture has been reported, it is...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910520625
更新日期:1992-12-02 00:00:00
abstract::Signal transduction pathways controlling tumor cell locomotion are not yet well understood. We have studied the role of protein kinase C (PKC)-dependent protein phosphorylation associated with changes in cell shape and locomotor activity of Walker carcinosarcoma cells in culture. We show that the inhibitory effect of ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0215(19960208)65:4<473::AID-IJC
更新日期:1996-02-08 00:00:00
abstract::In EBV-immortalized lymphoblastoid cell lines (LCLs) a small number of "latent" proteins are expressed. These are the EBV nuclear antigens, EBNAs 1-6, and a latent membrane protein, LMP. We have investigated the expression of these proteins in a variety of EBV-associated tumours and cell lines. Whereas transplant and ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910450623
更新日期:1990-06-15 00:00:00
abstract::Secreted frizzled-related proteins (SFRPs) are antagonists of the Wnt signaling pathway whose epigenetic downregulation have been shown to be involved in hepatocarcinogenesis. However, dysregulation of SFRPs induced by hepatitis B virus (HBV) X protein (HBx) has never been studied in HBV-related hepatocellular carcino...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.28697
更新日期:2014-08-01 00:00:00
abstract::In the setting of breast cancer screening, 5-9% of needle core biopsies are diagnosed as lesions of uncertain malignant potential (B3). The management of these lesions is potentially problematic as the data on their outcome remains limited. In our study, we aim to assess the outcome of screen-detected lesions diagnose...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.25801
更新日期:2011-09-15 00:00:00
abstract::High BCAR1/p130Cas expression in primary breast tumour cytosol predicts a poor chance of response recurrent disease to tamoxifen treatment in patients with oestrogen receptor (ER)-positive breast carcinomas. In this study, we assessed whether BCAR1/p130Cas expression is altered during acquisition of anti-oestrogen res...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/1097-0215(20000920)89:5<465::aid-ijc11>3.0
更新日期:2000-09-20 00:00:00
abstract::The European Study Group for Pancreatic Cancer (ESPAC-1) study is the largest study of adjuvant treatment for pancreatic ductal adenocarcinoma to date and confirmed a survival advantage for adjuvant chemotherapy but not for chemoradiation. The importance of parallel evaluation of survival and quality of life (QoL) has...
journal_title:International journal of cancer
pub_type: 杂志文章,随机对照试验
doi:10.1002/ijc.24270
更新日期:2009-06-15 00:00:00
abstract::GC-binding factor 2 (GCF2), a transcriptional repressor that decreases the activity of several genes is capable of binding directly to the GC-rich sequence of the EGFR promoter and repressing the transcriptional activity of EGFR. In addition to its function as a transcriptional repressor, GCF2 can directly interact wi...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.25837
更新日期:2011-10-01 00:00:00
abstract::Human papillomavirus (HPV) is a circular double-stranded DNA virus implicated in at least 90% of cervical and anogenital cancers and has been observed in approximately 20% of squamous cell carcinomas of the head and neck (SCCHN). Transcription of the viral oncogenes E6 and E7 is regulated by expression of the E2 prote...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.20193
更新日期:2004-07-10 00:00:00
abstract::The glycoprotein 90K was originally described as a tumor-secreted antigen and subsequently found to have immunostimulatory activity as well as other possible functions. This protein interacts with an endogenous lectin, galectin-3, and may play a role in tumor metastasis through this interaction. Because 90K is heavily...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/1097-0215(200002)9999:9999<::aid-ijc1022>3
更新日期:2001-01-15 00:00:00
abstract::A panel of 7 diagnostic cytopathologists, i.e., physicians trained to diagnose the malignant potential of human cells in Papanicolaou-stained smears, was asked to evaluate two sets of microscope slides of stained coverslip preparations of 28 cell culture lines, 15 of which were neoplastic. Slide Set I consisted of 13 ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910380310
更新日期:1986-09-15 00:00:00
abstract::Epigenetic silencing of MLH1 is the most common cause of defective DNA mismatch repair in endometrial and colorectal cancers. We hypothesized that variation in the MLH1 gene might contribute to the risk for MLH1 methylation and epigenetic silencing. We undertook a case-control study to test for the association between...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.22406
更新日期:2007-04-15 00:00:00
abstract::The metastatic dissemination of cancer cells to remote areas of the body is the most problematic aspect in cancer patients. Among cancers, melanomas are notoriously difficult to treat due to their significantly high metastatic potential even during early stages. Hence, the establishment of advanced therapeutic approac...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.31982
更新日期:2019-07-15 00:00:00
abstract::Loss of heterozygosity (LOH) at the site of the retinoblastoma (RB1) gene, at 13q14, has been shown to occur in a high proportion of ovarian cancer patients. Based on this, RB1 gene inactivation was studied in primary tumor cells from 15 patients with ovarian cancer. Structural changes as well as expression of the RB1...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910580508
更新日期:1994-09-01 00:00:00
abstract::Turkey poults were inoculated with lymphoproliferative disease virus (LPDV) of turkey, and the organotropism of the inoculated virus was determined from the kinetics of virus expression and tumor formation. Molecular hybridization experiments, conducted to determine the level of viral RNA expression in the various org...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910290518
更新日期:1982-05-15 00:00:00
abstract::ET-743, an experimental antitumor drug with promising activity in sarcoma, breast and ovarian carcinoma, is currently under phase 2 clinical evaluation. It is hepatotoxic in animals and patients. We tested the hypothesis that indole-3-carbinol (I3C), the hydrolysis product of glucosinolates occurring in cruciferous ve...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.20356
更新日期:2004-10-10 00:00:00
abstract::Human genes MAGE-1 and MAGE-3 code for distinct antigens, which are recognized on melanoma cells by autologous cytolytic T lymphocytes (CTL). These antigens may constitute useful targets for anti-cancer immunotherapy, since no expression of MAGE genes has been observed in normal tissues other than testis. Out of 57 sa...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910640112
更新日期:1995-02-20 00:00:00
abstract::We have recently shown in the BDII rat model of human endometrial adenocarcinoma (EAC), rat chromosome 10 (RNO10) is frequently involved in chromosomal aberrations. In the present study, we investigated the association between RNO10 deletions, allelic imbalance (AI) at RNO10q24 and Tp53 mutation in 27 rat EAC tumors. ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.22533
更新日期:2007-04-01 00:00:00
abstract::Interleukin (IL)-2 has been approved for treatment of metastatic renal cancer and malignant melanoma. However, its unfavorable pharmacologic properties, severe side effects and the negative role of IL-2 in maintaining T regulatory cells are severe drawbacks. It has been shown that immunocomplexes of IL-2 and certain a...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.25859
更新日期:2011-10-15 00:00:00
abstract::We obtained Thy-I-positive cells directly from growing methylcholanthrene-induced (MCA-1510) sarcomas using fluorescence-activated cell sorting, then cultured these lymphocytes in medium containing Interleukin-2 and tested their activity in vivo against various MCA-sarcoma lines with the Winn assay. We found that cult...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910280513
更新日期:1981-11-15 00:00:00
abstract::We examined pp60c-src protein kinase activity in human gastric carcinoma cell lines and gastric carcinoma tissues as well as normal mucosa. pp60c-src kinase activity was detected in all 5 carcinoma cell lines at various levels. Of 16 gastric carcinoma tissues, 8 showed higher pp60c-src kinase activity in tumor tissues...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910450510
更新日期:1990-05-15 00:00:00
abstract::The variants within the human melanocortin 1 receptor (MC1R) gene are associated with an increased risk of different skin cancers. In this study, we genotyped by direct sequencing, 529 cases of basal cell carcinoma of the skin (BCC) and 533 healthy controls for polymorphisms in the entire MC1R gene. In addition to 10 ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.23257
更新日期:2008-04-15 00:00:00
abstract::A general concern exists that cervical cancer screening using human papillomavirus (HPV) testing may lead to considerable overtreatment. We evaluated the trade-off between benefits and overtreatment among different screening strategies differing by primary tests (cytology, p16/Ki-67, HPV alone or in combinations), int...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.32849
更新日期:2020-08-15 00:00:00