Abstract:
:The purpose of this study was to determine whether the synthesis of endogenous nitric oxide (NO) is involved in the apoptosis of murine L929 transformed fibroblasts. L929 parental cells and L929 cells selected for resistance to tumor necrosis factor (TNF-alpha) were incubated in vitro with various concentrations of TNF-alpha, interleukin-1, and lipopolysaccharide (LPS) in the presence or absence of mouse interferon-gamma (IFN-gamma). The combination of subthreshold concentrations of IFN-gamma with the cytokines or LPS produced significant cell death within 48 h incubation. This cell death was associated with the induction of high levels of NO. Both cell death and NO production were significantly inhibited by the addition of N(G)-methyl-L-arginine (NMA), a specific inhibitor of nitric oxide synthase. The synergistic cytotoxicity was associated with extensive internucleosomal DNA fragmentation. NMA also inhibited this process. These data demonstrate the involvement of endogenous NO in cytokine-induced apoptosis of transformed cells.
journal_name
Int J Oncoljournal_title
International journal of oncologyauthors
Xie K,Huang S,Dong Z,Fidler Idoi
10.3892/ijo.3.6.1043subject
Has Abstractpub_date
1993-12-01 00:00:00pages
1043-8issue
6eissn
1019-6439issn
1791-2423journal_volume
3pub_type
杂志文章abstract::To investigate the usefulness of oral doxifluridine (5'-DFUR), an active intermediate metabolite of capecitabine (XELODA), in gastric cancer patients after curative resection, we conducted a phase III randomized controlled study to compare oral 5'-DFUR and oral 5-fluorouracil (5-FU). 485 gastric cancer patients with S...
journal_title:International journal of oncology
pub_type: 临床试验,杂志文章,随机对照试验
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更新日期:2000-05-01 00:00:00
abstract::We previously reported in a retrospective study that CEA-based RT-PCR was useful for predicting the prognosis of patients with node-negative colorectal cancer. RT-PCR is well established for laboratory use, but many issues remain to be resolved prior to its clinical application. In addition to the false positive rate ...
journal_title:International journal of oncology
pub_type: 杂志文章,多中心研究
doi:
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journal_title:International journal of oncology
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pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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abstract::HeLa cell cultures were used as model systems for small interfering RNA (siRNA) induced knockdown of mRNA expression of the human telomerase catalytic subunit, telomerase reverse transcriptase (hTERT). Four 21-bp siRNAs targeting different sites of the hTERT mRNA were designed, and the siRNA molecules produced by a T7...
journal_title:International journal of oncology
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abstract::Squamous cell carcinoma antigen (SCCA) is overexpressed in many squamous cell cancers and SCCA‑derived peptide-specific CD8(+) cytotoxic T lymphocytes can display cytotoxicity against tumor cells. In the present study, we screened the SCCA amino acid sequence for potential HLA-A*0201-binding CD8(+) T‑cell epitopes usi...
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journal_title:International journal of oncology
pub_type: 杂志文章,评审
doi:10.3892/ijo.2013.2104
更新日期:2013-11-01 00:00:00
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journal_title:International journal of oncology
pub_type: 杂志文章
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更新日期:2003-12-01 00:00:00
abstract::The p53 homologue p73 is overexpressed in many tumors, including lung cancer. We have evaluated the differential expression and subcellular localization of the functionally distinct apoptotic (TA) and anti-apoptotic (DeltaN) isoforms of p73 in non-small cell lung cancer (NSCLC), their possible association with p53 exp...
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