Effect of tear hyperosmolarity and signs of clinical ocular surface pathology upon conjunctival goblet cell function in the human ocular surface.

abstract：PURPOSE:Erythropoietin (EPO) modulates erythropoiesis by inhibiting apoptosis in erythrocyte progenitors. Recently, EPO has been shown to be protective in experimental models of mechanical trauma, neuroinflammation, cerebral and retinal ischemia, and even in a human stroke trial. However, little is known about EPO sign...

journal_title：Investigative ophthalmology & visual science

authors： Weishaupt JH,Rohde G,Pölking E,Siren AL,Ehrenreich H,Bähr M

更新日期：2004-05-01 00:00:00

abstract：PURPOSE:To explore changes in corneal sensitivity that develop in patients with dry eye and the relationship between sensibility and severity of the dry eye disease. METHODS:Experiments were performed in 44 patients with dry eye and 42 healthy individuals. Corneal sensitivity was measured with the Belmonte noncontact ...

journal_title：Investigative ophthalmology & visual science

authors： Bourcier T,Acosta MC,Borderie V,Borrás F,Gallar J,Bury T,Laroche L,Belmonte C

更新日期：2005-07-01 00:00:00

abstract：PURPOSE:This was a pilot study conducted to examine the expression of osteopontin in uveal melanoma and to determine whether serum osteopontin can be used in detecting metastatic uveal melanoma. METHODS:Osteopontin mRNA was measured in three uveal melanoma cell lines of various invasive potential by real-time PCR. Tis...

journal_title：Investigative ophthalmology & visual science

authors： Kadkol SS,Lin AY,Barak V,Kalickman I,Leach L,Valyi-Nagy K,Majumdar D,Setty S,Maniotis AJ,Folberg R,Pe'er J

更新日期：2006-03-01 00:00:00

abstract：PURPOSE:It is thought that large perimetric stimuli are insensitive for demonstrating visual field defects. To test the hypothesis that there is no difference in the total number of abnormal test locations with total deviation empiric probability plots in glaucoma patients, we compared results of glaucoma patients test...

journal_title：Investigative ophthalmology & visual science

authors： Wall M,Doyle CK,Eden T,Zamba KD,Johnson CA

更新日期：2013-06-07 00:00:00

abstract：PURPOSE:Strabismus that develops shortly after birth is known to cause temporal-to-nasal eye movement asymmetries under monocular viewing. The neural mechanisms underlying this deficit are not well understood. In the current study, the hypothesis that this eye movement anomaly reflects a similar asymmetry in the direct...

journal_title：Investigative ophthalmology & visual science

authors： Watanabe I,Bi H,Zhang B,Sakai E,Mori T,Harwerth RS,Smith EL 3rd,Chino YM

更新日期：2005-10-01 00:00:00

abstract：Purpose:Dense deposit disease (DDD) is caused by dysregulation of the alternative pathway of the complement cascade and characterized by electron-dense deposits in the kidney glomerular basement membrane (GBM) and drusen in Bruch's membrane (BrM). Complement factor H (fH) and factor properdin (fP) regulate complement a...

journal_title：Investigative ophthalmology & visual science

authors： Song D,Mohammed I,Bhuyan R,Miwa T,Williams AL,Gullipalli D,Sato S,Song Y,Dunaief JL,Song WC

更新日期：2018-07-02 00:00:00

abstract：PURPOSE:Point and null mutations in interphotoreceptor retinoid-binding protein (IRBP) cause retinal dystrophy in affected patients and IRBP-deficient mice with unknown mechanism. This study investigated whether IRBP protects cells from damages induced by all-trans-retinal (atRAL), which was increased in the Irbp(-/-) ...

journal_title：Investigative ophthalmology & visual science

authors： Lee M,Li S,Sato K,Jin M

更新日期：2016-04-01 00:00:00

abstract：PURPOSE:To determine the roles played by toll-like receptor 9 (TLR9) in cultured human corneal endothelial (HCEn) cells after herpes simplex virus type 1 (HSV-1) infection and to characterize the TLR9-mediated antiviral responses. METHOD:Immortalized HCEn cells were examined for TLR expression. The upregulation of inf...

journal_title：Investigative ophthalmology & visual science

authors： Takeda S,Miyazaki D,Sasaki S,Yamamoto Y,Terasaka Y,Yakura K,Yamagami S,Ebihara N,Inoue Y

更新日期：2011-08-24 00:00:00

abstract：Purpose:We assess if α retinal ganglion cells (αRGCs) and intrinsically photosensitive retinal ganglion cells (ipRGCs) survive in mouse models of glaucoma. Methods:Two microliters of N-methyl-D-aspartate (NMDA; 1 mM) or PBS were injected intraocularly 7 days before sacrifice. Immunohistochemical analyses of the retina...

journal_title：Investigative ophthalmology & visual science

authors： Honda S,Namekata K,Kimura A,Guo X,Harada C,Murakami A,Matsuda A,Harada T

更新日期：2019-09-03 00:00:00

abstract：PURPOSE:To investigate the role of N-cadherin and hepatocyte growth factor (HGF) in the invasion of collagen type I by human retinal pigment epithelial (RPE) cells. METHODS:RPE sheets from eight human eyes were used for characterization through Western blot analysis of the expression of cadherin in total lysates or af...

journal_title：Investigative ophthalmology & visual science

authors： Van Aken EH,De Wever O,Van Hoorde L,Bruyneel E,De Laey JJ,Mareel MM

更新日期：2003-02-01 00:00:00

abstract：:Herpes simplex virus type 1 (HSV-1) inoculated directly into the anterior chamber of the mouse eye induced an acute inflammatory process in both the injected eye and its uninjected (contralateral) counterpart. In the former, a rapid intense inflammatory reaction developed in the anterior segment (cornea, anterior cham...

journal_title：Investigative ophthalmology & visual science

authors： Whittum JA,McCulley JP,Niederkorn JY,Streilein JW

更新日期：1984-09-01 00:00:00

abstract：PURPOSE:To investigate the residual stereo function of a group of 15 patients with strabismic amblyopia, by using motion-in-depth stimuli that allow discrimination of contributions from local disparity as opposed to those from local velocity mechanisms as a function of the rate of depth change. METHODS:The stereo perf...

journal_title：Investigative ophthalmology & visual science

authors： Hess RF,Mansouri B,Thompson B,Gheorghiu E

更新日期：2009-10-01 00:00:00

abstract：PURPOSE:The aim of this study was to show the usefulness of three methods for measuring IOP: Goldmann applanation tonometry, rebound tonometry, and Ultra-High-Speed Scheimpflug technology. METHODS:The examined group consisted of 96 patients (192 eyes), including 63 women and 33 men with a mean age of 59.3 ± 19.9 years...

journal_title：Investigative ophthalmology & visual science

authors： Smedowski A,Weglarz B,Tarnawska D,Kaarniranta K,Wylegala E

更新日期：2014-02-04 00:00:00

abstract：PURPOSE:CD40 antigen is a membrane receptor that plays a role in the regulation of immune reactions. The expressions of CD40 and CD40 ligand (CD40L) were investigated ex vivo and in vitro in conjunctival epithelial cells, in correlation with HLA DR class H antigen, previously shown to be upregulated in conjunctival inf...

journal_title：Investigative ophthalmology & visual science

authors： Bourcier T,De Saint-Jean M,Brignole F,Goguel A,Baudouin C

更新日期：2000-01-01 00:00:00

abstract：PURPOSE:To evaluate changes in the multifocal electroretinogram (mfERG) in patients with neovascular age-related macular degeneration (nAMD) undergoing ranibizumab treatment. METHODS:This was an observational, longitudinal, prospective study. Treatment-naive patients with nAMD who met the inclusion and exclusion crite...

journal_title：Investigative ophthalmology & visual science

authors： Campa C,Hagan R,Sahni JN,Brown MC,Beare NA,Heimann H,Harding SP

更新日期：2011-06-01 00:00:00

abstract：:Posterior eye cups of the frog Rana ridibunda were prefixed in the presence of tannic acid, osmicated and stained en bloc with uranyl acetate. Electron microscopy revealed the following features of membrane traffic in cells of the retinal pigment epithelium (RPE): (1) intracellular membranes with increased electron de...

journal_title：Investigative ophthalmology & visual science

authors： Rungger-Brändle E,Englert U,Leuenberger PM

更新日期：1987-12-01 00:00:00

abstract：:Cataracts in cultured rabbit lenses were produced by elevation of internal calcium. Experimental procedures were successful in increasing levels of total and bound Ca++, often without significant changes in sodium, potassium, or water content. Although the excess in calcium was predominantly associated with water-solu...

journal_title：Investigative ophthalmology & visual science

authors： Hightower KR,Reddy VN

更新日期：1982-02-01 00:00:00

abstract：PURPOSE:Current understanding of the genetic risk factors for age-related macular degeneration (AMD) is not sufficiently predictive of the clinical course. The VEGF pathway is a key therapeutic target for treatment of neovascular AMD; however, risk attributable to genetic variation within pathway genes is unclear. We s...

journal_title：Investigative ophthalmology & visual science

authors： Owen LA,Morrison MA,Ahn J,Woo SJ,Sato H,Robinson R,Morgan DJ,Zacharaki F,Simeonova M,Uehara H,Chakravarthy U,Hogg RE,Ambati BK,Kotoula M,Baehr W,Haider NB,Silvestri G,Miller JW,Tsironi EE,Farrer LA,Kim IK,Park K

更新日期：2014-05-08 00:00:00

abstract：PURPOSE:To optimize a nonviral gene transfection system targeting the corneal limbal stem/progenitor cells. METHODS:A plasmid containing LacZ gene coding for beta-galactosidase (beta-gal) was transfected into human corneal epithelial cells (HCECs) and multilineage progenitor cells (MLPCs) with different transfection r...

journal_title：Investigative ophthalmology & visual science

authors： Zhao B,Allinson SL,Ma A,Bentley AJ,Martin FL,Fullwood NJ

更新日期：2008-08-01 00:00:00

abstract：PURPOSE:To investigate the effect of corneal trauma on gene expression in the lacrimal gland and to assess how many genes and what specific genes are regulated in response to corneal trauma. METHODS:A mouse model with acute corneal trauma was created with a chemical burn to the cornea with silver nitrate. Sixty-four f...

journal_title：Investigative ophthalmology & visual science

authors： Fang Y,Choi D,Searles RP,Mathers WD

更新日期：2005-02-01 00:00:00

abstract：:Retinoblastoma patients have cellular and humoral reactivity towards antigens expressed on retinoblastoma cells. We report the ultrastructural, cytogenetic, and immunologic features of four new retinoblastoma derived tissue culture cell lines. Studies with hybridoma produced monoclonal antibodies demonstrate that thes...

journal_title：Investigative ophthalmology & visual science

authors： Char DH,Wood IS,Huhta K,Rand N,Morita CT,Howes EL Jr

更新日期：1984-01-01 00:00:00

abstract：PURPOSE:To elucidate the role of signals mediated by EphB4 receptor tyrosine kinase and its transmembrane ephrinB2 ligand in corneal lymphatic capillaries. METHODS:To detect expression of ephrinB2 and EphB4 in mouse corneas, immunohistochemistry of flat-mount corneas from 6- to 10-week-old wild-type, Efnb2-lacZ, and E...

journal_title：Investigative ophthalmology & visual science

authors： Katsuta H,Fukushima Y,Maruyama K,Hirashima M,Nishida K,Nishikawa S,Uemura A

更新日期：2013-06-12 00:00:00

abstract：:Purpose. To use optical coherence tomography (OCT) to evaluate the effect of punctal occlusion on tear meniscus volume in dry eye patients. Methods. Occlusion of both upper and lower puncta with collagen plugs was performed on one eye each in 20 dry eye patients and 20 normal subjects. The upper and lower tear menisci...

journal_title：Investigative ophthalmology & visual science

authors： Chen F,Shen M,Chen W,Wang J,Li M,Yuan Y,Lu F

更新日期：2010-04-01 00:00:00

abstract：PURPOSE:To examine the association between central corneal thickness (CCT) and glaucoma. METHODS:This was a nested case-control study using 1090 subjects from an eye disease population survey in Singapore and 243 participants from a hospital glaucoma surgery clinical trial in Singapore. RESULTS:Mean CCT in 938 commun...

journal_title：Investigative ophthalmology & visual science

authors： Day AC,Machin D,Aung T,Gazzard G,Husain R,Chew PT,Khaw PT,Seah SK,Foster PJ

更新日期：2011-10-28 00:00:00

abstract：:Herpes simplex virus type 1 (HSV-1) can infect the cornea and achieve ganglionic latency. HSV-1 can later be activated by a variety of effectors although the exact mechanism of reactivation is unknown. Rabbits harboring latent HSV-1 strain McKrae can be induced to shed virus by ocular iontophoresis of epinephrine to t...

journal_title：Investigative ophthalmology & visual science

authors： Rootman DS,Haruta Y,Hill JM,Kaufman HE

更新日期：1988-03-01 00:00:00

abstract：UNLABELLED:PURPOSE. Test the hypothesis that the developmental increases in rod photoreceptor sensitivity and rod-mediated visual sensitivity at 10 degrees, 20 degrees , and 30 degrees eccentric are concurrent. It is known that maturation of the parafoveal (10 degrees eccentric) rod outer segments and visual sensitivit...

journal_title：Investigative ophthalmology & visual science

authors： Fulton AB,Hansen RM

更新日期：2000-05-01 00:00:00

abstract：PURPOSE:To investigate the relationship between long-term glycemic control and photopic negative response (PhNR) changes in the blue flash ERG in adolescents with type 1 diabetes (T1D) without diabetic retinopathy (DR). METHODS:After light adaptation, ERG responses to 1.60 cd·s/m(2) blue (420 nm) flashes (blue flash E...

journal_title：Investigative ophthalmology & visual science

authors： McFarlane M,Wright T,Stephens D,Nilsson J,Westall CA

更新日期：2012-02-13 00:00:00

abstract：:Spleen cells of BALB/c mice, previously immunized with bovine retinal S-antigen, were fused with Sp2/0-Ag14 mouse myeloma cells. Two monoclonal antibodies (MAbs) MAbA9-C6 (IgG2a isotype) and MAbA1-G5 (IgG1 isotype) were selected on the basis of reactivity in an ELISA and immunofluorescent assay. In radioimmunoassay MA...

journal_title：Investigative ophthalmology & visual science

authors： Donoso LA,Merryman CF,Edelberg KE,Naids R,Kalsow C

更新日期：1985-04-01 00:00:00

abstract：PURPOSE:This study aims to determine the in vivo effectiveness of low-frequency ultrasound in mediating the transport of macromolecules to the posterior segment of the eye via transscleral route. It investigates if damage is caused by ultrasound at the tested operation parameters on the posterior ocular tissues and vis...

journal_title：Investigative ophthalmology & visual science

authors： Suen WL,Wong HS,Yu Y,Lau LC,Lo AC,Chau Y

更新日期：2013-06-26 00:00:00

abstract：:The neutrophilic response in the alkali-injured rabbit cornea can be separated into two peaks: the first from 12-24 hr and the second at 21 days. In the experiments detailed here, alkali-injured rabbit eyes were treated for 0-7 days (early peak), 6-35 days (late peak), or 0-35 days (both peaks) with 6.5% or 10% topica...

journal_title：Investigative ophthalmology & visual science

authors： Haddox JL,Pfister RR,Yuille-Barr D

更新日期：1989-06-01 00:00:00