Abstract:
:The neutrophilic response in the alkali-injured rabbit cornea can be separated into two peaks: the first from 12-24 hr and the second at 21 days. In the experiments detailed here, alkali-injured rabbit eyes were treated for 0-7 days (early peak), 6-35 days (late peak), or 0-35 days (both peaks) with 6.5% or 10% topical citrate. Clinical evaluation of these eyes showed that treatment with 10% citrate (0-35 days) significantly reduced the incidence and severity of corneal ulceration from the control level. When 10% citrate was administered to block the early neutrophilic peak, the incidence of corneal ulceration was also significantly reduced (33.3% from 72.2%). The average ulcer depth increased gradually, reaching an intermediate level well below the control group by day 35. In contrast, 10% citrate treatment of the late peak had no significant effect on the incidence of ulceration; however, these ulcers were less severe than those in the control. When 6.5% topical citrate (0-35 days) was used, there was a significant reduction in the severity but not the incidence of ulceration. In addition, the beneficial effects obtained from separate treatment of the early and late PMN peaks were mostly lost. These experiments suggest that both the early and late peak of the PMN response after alkali injury are important to the development of corneal ulcers. We hypothesize that the early use of 10% citrate diminishes the initial peak of PMNs, interfering with the release of self-perpetuating inflammatory mediators, thereby decreasing the later accumulation of PMNs that cause ulceration.
journal_name
Invest Ophthalmol Vis Scijournal_title
Investigative ophthalmology & visual scienceauthors
Haddox JL,Pfister RR,Yuille-Barr Dsubject
Has Abstractpub_date
1989-06-01 00:00:00pages
1062-8issue
6eissn
0146-0404issn
1552-5783journal_volume
30pub_type
杂志文章abstract:PURPOSE:The purpose of this study was to evaluate the Tono-Pen 2 tonometer for measuring intraocular pressure (IOP) in the living rat eye. METHODS:One eye from each of 20 adult, anesthetized brown Norway rats (group 1) was cannulated and simultaneously connected to a syringe and a pressure transducer with a chart reco...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:
更新日期:1993-02-01 00:00:00
abstract::The definition and diagnostic criteria of dry eye disease (DED) proposed by the Japan Dry Eye Society and other countries are reviewed. The first definition and criteria of DED in Japan were proposed in 1995. In that report, DED was considered a disease of tears, which subsequently damaged ocular epithelia. The presen...
journal_title:Investigative ophthalmology & visual science
pub_type: 历史文章,杂志文章,评审
doi:10.1167/iovs.17-23475
更新日期:2018-11-01 00:00:00
abstract:PURPOSE:The inherited early-onset macular degenerative disease known as malattia leventinese (ML) and Doyne honeycomb retinal dystrophy (DHRD) have been linked to a missense mutation leading to production of a mutant fibulin-3 protein (R345W). R345W is poorly secreted and accumulates in the RPE of ML/DHRD retinas. Accu...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.05-0070
更新日期:2005-11-01 00:00:00
abstract:PURPOSE:Generate site-specific somatic mutations selectively in the retinal pigment epithelium (RPE) in mice. METHODS:A transgenic mouse line expressing the Cre recombinase under the control of the tyrosinase-related protein (TRP)-1 promoter was generated. The presence of Cre was determined by in situ hybridization, a...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:
更新日期:2002-05-01 00:00:00
abstract:PURPOSE:The Rd4/+ mouse inherits an autosomal dominant retinal degeneration that cosegregates with a large inversion spanning nearly all of mouse chromosome 4 (Chr 4). This inversion is homozygous lethal. The hypothesis for the study was that disruption of a gene at one of the two breakpoints in the Rd4 chromosome is r...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.05-1164
更新日期:2006-04-01 00:00:00
abstract::Secretory component (SC) was studied by indirect immunofluorescence of the ocular surface epithelium. We find that conjunctival epithelium produces this component, and that it is absent in the corneal epithelium. Conjunctival epithelium loses its SC staining within 1 to 2 days as it grows over a denuded corneal stroma...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:
更新日期:1981-01-01 00:00:00
abstract::A method of analysing fluorescein angiograms that yields a parameter of intraocular blood flow designated as the fluorescein appearance rate is presented. The appearance rate was decreased in those eyes in which intraocular pressure (Pi) was found to be increased. The appearance rate was also decreased in the two case...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:
更新日期:1983-03-01 00:00:00
abstract:Purpose:To evaluate the relationship between refractive error, circadian phase, and melatonin with consideration of prior light exposure, physical activity, and sleep. Methods:Healthy young myopic (spherical equivalent refraction [SER] ≤-0.50DS) and emmetropic adults underwent noncycloplegic autorefraction and axial l...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.61.8.45
更新日期:2020-07-01 00:00:00
abstract::Superoxide dismutase (SOD) molecules occur in all cells exposed to an oxygen-containing environment, including retinal pigment epithelial (RPE) cells. Previous studies of nonhuman RPE have either probed specifically for copper-zinc-containing SOD (CuZn-SOD) or have not distinguished between CuZn-SOD and the SOD molecu...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:
更新日期:1990-12-01 00:00:00
abstract:PURPOSE:To evaluate the efficiency, cell specificity, stability, and toxicity of recombinant adeno-associated virus (rAAV)-mediated retinal transduction in vivo in the adult immunocompetent mouse. To assess the usefulness of green fluorescent protein (GFP) for real-time, noninvasive monitoring of retinal transgene expr...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:
更新日期:1997-12-01 00:00:00
abstract:PURPOSE:To develop an imaging technique that maps the distribution of free cystine in the rat lens at subcellular resolution and enables cystine accumulation to be compared with the expression of the cystine-glutamate exchanger (Xc-). METHODS:Whole lenses were fixed, cryoprotected, and then cryosectioned in either an ...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.06-0861
更新日期:2007-03-01 00:00:00
abstract:PURPOSE:Intramuscular innervation of horizontal rectus extraocular muscles (EOMs) is segregated into superior and inferior (transverse) compartments, while all EOMs are also divided into global (GL) and orbital (OL) layers with scleral and pulley insertions, respectively. We sought evidence of potential independent act...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.12-10318
更新日期:2012-12-19 00:00:00
abstract:PURPOSE:In corneal endothelial cells, intercellular Ca(2+) waves elicited by a mechanical stimulus involve paracrine intercellular communication, mediated by ATP release via connexin hemichannels, as well as gap junctional intercellular communication. Both mechanisms are inhibited by thrombin, which activates RhoA and ...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.06-1062
更新日期:2007-04-01 00:00:00
abstract:PURPOSE:To evaluate the differential effects of ganglion cell depletion from experimental glaucoma on the relative metabolic activities of neurons in the parvo (P)- and magno (M)-cellular visual pathways of the macaque visual system. METHODS:Monocular experimental glaucoma was induced in monkeys (Macaca mulatta and M....
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:
更新日期:2000-06-01 00:00:00
abstract:PURPOSE:To compare self-assessed driving habits and skills of licensed drivers with central visual loss who use bioptic telescopes to those of age-matched normally sighted drivers, and to examine the association between bioptic drivers' impressions of the quality of their driving and ratings by a "backseat" evaluator. ...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.13-13520
更新日期:2014-01-15 00:00:00
abstract:PURPOSE:To determine the role of small GTPase Rho and its relation with epidermal growth factor receptor (EGFR) in mediating corneal epithelial wound healing. METHODS:Rho activity in THCE cells, an SV40-immortalized human corneal epithelial cell (HCEC) line, and primary HCECs was assessed by pull-down assay followed b...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.07-1122
更新日期:2008-03-01 00:00:00
abstract::Fixation stability of the saccadic system was investigated by infrared reflection oculography in 29 normal subjects, young and elderly. Square wave jerks consisting of spontaneous horizontal saccadic excursions of 0.5 degree and over, followed some 200 msec later by corrective saccades, were recorded in 24% of subject...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:
更新日期:1981-02-01 00:00:00
abstract:PURPOSE:To characterize photoreceptor cell apoptosis and cell loss in a mouse model of experimental retinal detachment (RD), and to use the technology of mouse genetics to study the molecular mechanisms underlying RD-associated photoreceptor degeneration. METHODS:Retinal detachments were created in adult wild-type and...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.03-0827
更新日期:2004-02-01 00:00:00
abstract::Topically applied dipivalyl epinephrine (DPE) and related compounds have been found to inhibit passive anaphylactic reaction in rat conjunctiva. The order of activity is as follows: isoproterenol greater than DPE greater than epinephrine greater than norepinephrine. The antianaphylactic effect of DPE was antagonized b...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:
更新日期:1980-07-01 00:00:00
abstract:PURPOSE:Müller glia play crucial roles in retinal homeostasis and function. Genetic modification of Müller cells by viral gene delivery would be valuable for studies of their normal physiology and roles in retinal disease states. However, stable and efficient transgene expression in Müller cells after delivery of gene ...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.05-1570
更新日期:2007-04-01 00:00:00
abstract:PURPOSE:To determine the possible antiangiogenic effect of metalloproteinase (MMP) 14 cleavage of vascular endothelial growth factor receptor 1 (VEGFR1) in the cornea. METHODS:Recombinant mouse (rm) VEGFR1 was incubated with various concentrations of recombinant MMP14 to examine proteolysis in vitro. The reaction mixt...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.14-16248
更新日期:2015-08-01 00:00:00
abstract:PURPOSE:The association of age and blood pressure (BP) with retinal vessel change is widely reported, with inverse relationships between retinal arteriolar and venular diameter and increasing age and elevated BP. No previous studies have dissected the separate effects of age and BP on the diameter of retinal vessels. ...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.06-0893
更新日期:2007-02-01 00:00:00
abstract:PURPOSE:To characterize the chemokines and chemokine receptors that mediate the effect of proinflammatory cytokines, interleukin (IL)-1 and tumor necrosis factor (TNF)-alpha, on the recruitment of MHC class II(+) Langerhans cells (LCs) in the corneal epithelium. METHODS:A standard model for corneal LC recruitment, app...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.04-0658
更新日期:2005-04-01 00:00:00
abstract::To investigate the accumulation of tear proteins on disposable extended-wear contact lenses (42% Etafilcon A and 58% hydration), a technique involving sodium dodecyl sulfate-polyacrylamide minigel electrophoresis combined with a sensitive silver-staining method was used. Besides the binding of large amounts of tear ly...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:
更新日期:1990-11-01 00:00:00
abstract::The free epsilon-amino groups and 5-hydroxymethylfurfural (5-HMF) contents were determined in soluble and insoluble proteins of clear human lenses and diabetic and nondiabetic senile cataractous lenses. The free epsilon-amino group content of soluble proteins in diabetic cataracts was decreased by 37% (P less than 0.0...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:
更新日期:1986-01-01 00:00:00
abstract:PURPOSE:To evaluate the intraocular safety and pharmacokinetics of hexadecyloxypropyl-cidofovir (HDP-CDV), the hydrolysis product of HDP-cyclic-CDV, a long-lasting intravitreal cidofovir prodrug for cytomegalovirus (CMV) retinitis. METHODS:HDP-cyclic-CDV was suspended in phosphate-buffered saline (PBS) at 37°C and for...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.11-8293
更新日期:2011-12-09 00:00:00
abstract:Purpose:Dense deposit disease (DDD) is caused by dysregulation of the alternative pathway of the complement cascade and characterized by electron-dense deposits in the kidney glomerular basement membrane (GBM) and drusen in Bruch's membrane (BrM). Complement factor H (fH) and factor properdin (fP) regulate complement a...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.18-24133
更新日期:2018-07-02 00:00:00
abstract:PURPOSE:The efficacy of the peptide Ac-EEED on reducing cell adhesion and proliferation in vitro and choroidal neovascularization (CNV) in vivo was examined. METHODS:The peptide chimera containing the Ac-EEED sequence was chemically linked to the N terminus of the XMTM delivery peptide from the E(rns) viral surface pr...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.10-6351
更新日期:2011-04-16 00:00:00
abstract:PURPOSE:The epidermal growth factor receptor (EGFR) appears in astrocytes after neural injury. The authors' laboratory has reported the presence of EGFR in glaucomatous optic nerves. The activation of EGFR is often associated with induction of cyclooxygenase (COX)-2. In this study, the induction of COX-2 pathway in rat...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.04-1473
更新日期:2005-06-01 00:00:00
abstract:PURPOSE:To identify the chromosomal location of the gene causing snowflake vitreoretinal degeneration (SVD), an autosomal dominant retinal degeneration characterized by small yellow-white dots in the retina, fibrillar anomaly of the vitreous humor, and retinal detachment. METHODS:Clinical data were collected on 31 fam...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.04-0722
更新日期:2004-12-01 00:00:00