当前位置： SCI文献检索 > PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA期刊下所有文献 > Active liquid-like behavior of nucleoli determines their size and shape in Xenopus laevis oocytes.

Active liquid-like behavior of nucleoli determines their size and shape in Xenopus laevis oocytes.

Abstract：

:For most intracellular structures with larger than molecular dimensions, little is known about the connection between underlying molecular activities and higher order organization such as size and shape. Here, we show that both the size and shape of the amphibian oocyte nucleolus ultimately arise because nucleoli behave as liquid-like droplets of RNA and protein, exhibiting characteristic viscous fluid dynamics even on timescales of < 1 min. We use these dynamics to determine an apparent nucleolar viscosity, and we show that this viscosity is ATP-dependent, suggesting a role for active processes in fluidizing internal contents. Nucleolar surface tension and fluidity cause their restructuring into spherical droplets upon imposed mechanical deformations. Nucleoli exhibit a broad distribution of sizes with a characteristic power law, which we show is a consequence of spontaneous coalescence events. These results have implications for the function of nucleoli in ribosome subunit processing and provide a physical link between activity within a macromolecular assembly and its physical properties on larger length scales.

journal_name

Proc Natl Acad Sci U S A

journal_title

Proceedings of the National Academy of Sciences of the United States of America

authors

Brangwynne CP,Mitchison TJ,Hyman AA

doi

10.1073/pnas.1017150108

subject

Has Abstract

pub_date

2011-03-15 00:00:00

pages

4334-9

issue

11

eissn

0027-8424

issn

1091-6490

pii

1017150108

journal_volume

108

pub_type

杂志文章

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