Abstract:
:To define the gene content of the HLA class I region, cDNA selection was applied to three overlapping yeast artificial chromosomes (YACs) that spanned 1 megabase (Mb) of this region of the human major histocompatibility complex. These YACs extended from the region centromeric to HLA-E to the region telomeric to HLA-F. In addition to the recognized class I genes and pseudogenes and the anonymous non-class-I genes described recently by us and others, 20 additional anonymous cDNA clones were identified from this 1-Mb region. We also identified a long repetitive DNA element in the region between HLA-B and HLA-E. Homologues of this element were located at several sites in the human genome outside of the HLA complex. The portion of the HLA class I region represented by these YACs shows an average gene density as high as the class II and class III regions. Thus, the high gene density portion of the HLA complex is extended to more than 3 Mb.
journal_name
Proc Natl Acad Sci U S Aauthors
Wei H,Fan WF,Xu H,Parimoo S,Shukla H,Chaplin DD,Weissman SMdoi
10.1073/pnas.90.24.11870subject
Has Abstractpub_date
1993-12-15 00:00:00pages
11870-4issue
24eissn
0027-8424issn
1091-6490journal_volume
90pub_type
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