Abstract：
OBJECTIVE:To improve posttransplantation survival of frozen-thawed human ovarian tissue in immunodeficient mice. DESIGN:Histologic study of transplanted human ovaries after treating the host and graft. SETTING:Infertility unit, university-affiliated tertiary medical center. PATIENT(S):Ovarian tissue from six girls/women (aged 5-23 years) who had undergone ovarian laparoscopy for fertility preservation. INTERVENTION(S):None. MAIN OUTCOME MEASURE(S):Thawed ovarian samples were transplanted into the back muscle of immunodeficient mice divided into four groups: A) no treatment; B) host treatment with vitamin E and gonadotropins before and after grafting; C) graft incubation with vascular endothelial growth factor A (VEGF-A) and vitamin E before transplantation; and D) host as in B, graft as in C. Ungrafted thawed samples served as control. Assessment of graft survival was conducted by follicle counts, apoptosis evaluation, immunohistochemical stainings for proliferating cell nuclear antigen (PCNA) and VEGF-A expression. RESULT(S):Only grafts incubated before transplantation (groups C and D) retained their original size. Follicle number was low in all grafts. PCNA expression was found in most grafts. Apoptosis was significantly lower in the untreated and treated grafts transplanted into treated hosts (groups B and D) than in ungrafted-thawed samples and group A grafts. All grafted groups had significantly higher expression of VEGF-A than ungrafted-thawed samples. CONCLUSION(S):Survival of transplanted human ovarian tissue may be improved by treatment of the host and graft. Further studies to evaluate treatments with a potential benefit in human ovarian autotransplantation are needed.
journal_name
Fertil Steriljournal_title
Fertility and sterilityauthors
Abir R,Fisch B,Jessel S,Felz C,Ben-Haroush A,Orvieto Rdoi
10.1016/j.fertnstert.2010.07.1082subject
Has Abstractpub_date
2011-03-15 00:00:00pages
1205-10issue
4eissn
0015-0282issn
1556-5653pii
S0015-0282(10)02208-9journal_volume
95pub_type
杂志文章abstract：OBJECTIVE:To determine whether self-selected women with polycystic ovary syndrome (PCOS) are abnormal compared with a control population. DESIGN:Case-control. SETTING:Support group meeting organized and initiated by patients. PATIENT(S):Forty-five self-selected women with PCOS and 80 control women. INTERVENTION(S):...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(02)03153-9
更新日期：2002-07-01 00:00:00
abstract：OBJECTIVE:To show the responsiveness of tissue-engineered human endometrial stroma to combinations of hormones that mimic the secretory and menstrual phases of the cycle. DESIGN:In vitro experimental study. SETTING:University uterine biology research laboratory. PATIENT(S):None. INTERVENTION(S):Telomerase immortali...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2012.01.098
更新日期：2012-04-01 00:00:00
abstract：OBJECTIVE:To determine whether fetal fibronectin (FFN) might serve as a marker to distinguish intrauterine versus extrauterine pregnancy. DESIGN:Prospective cohort study. SETTING:Academic research center. PATIENT(S):Cervicovaginal FFN samples were obtained from 46 women who were at high risk for or presented with si...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(99)00224-1
更新日期：1999-08-01 00:00:00
abstract：:Live and dead spermatozoa of human semen were distinguished and evaluated separately with respect to their morphology after supravital staining with buffered eosin. This evaluation was shown to be almost as reliable as that of the commonly stained smear. Different samples from the same individual were found to be simi...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)42377-0
更新日期：1977-02-01 00:00:00
abstract：OBJECTIVE:To evaluate how endometriosis affects expression of vascular endothelial growth factor (VEGF) and interleukin-6 (IL-6) in granulosa cells. DESIGN:Prospective study. SETTING:IVF-ET program at Osaka Medical College. PATIENT(S):Seventeen patients with revised American Fertility Society stage IV endometriosis ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(02)03339-3
更新日期：2002-10-01 00:00:00
abstract：:Chronic treatment with highly active analogs of luteinizing hormone (LH)-releasing hormone (LH-RH) induces "paradoxical" antifertility effects. Intact male rats, a reduction in testosterone production is observed after the administration of [D-Ser(But)6]-LH-RH(1-9)ethylamide (burserelin, Hoe 766), 50 ng/day subcutaneo...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)43461-8
更新日期：1978-08-01 00:00:00
abstract：OBJECTIVE:To determine whether it is possible to identify and diagnose accurately Müllerian anomalies with three-dimensional (3-D) ultrasound (US). DESIGN:Controlled blinded clinical study. SETTING:Normal human volunteers undergoing infertility evaluation in a university hospital. PATIENTS:Forty-two patients who und...
journal_title：Fertility and sterility
pub_type： 临床试验,杂志文章
doi：10.1016/s0015-0282(16)58147-3
更新日期：1996-03-01 00:00:00
abstract：:A new direct-to-consumer genetic test purports to detect fetal gender as early as five weeks of pregnancy. The availability of this test highlights the consequences of the current system of oversight for genetic tests and underscores the need for an enhanced regulatory framework to ensure the accuracy, reliability, an...
journal_title：Fertility and sterility
pub_type： 社论
doi：10.1016/j.fertnstert.2006.03.016
更新日期：2006-07-01 00:00:00
abstract：OBJECTIVE:To discuss the new peer review system at the National Institutes of Health (NIH) and to present the process undertaken to accomplish these changes, with an emphasis on the reproductive sciences. DESIGN:A discussion of the many NIH committees and their composition and reports leading to the changes in peer re...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2003.10.006
更新日期：2004-02-01 00:00:00
abstract：OBJECTIVE:To evaluate whether the presence of kissing ovaries at ultrasound is a marker for endometriosis and whether it correlates with the severity of the disease. DESIGN:Prospective observational study. SETTING:Gynecologic departments of two university hospitals. PATIENT(S):A total of 722 consecutive premenopausa...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2004.05.094
更新日期：2005-01-01 00:00:00
abstract：:Our studies demonstrate significantly lower expression of relaxin and its receptor in ectopic endometriotic tissues than their expression in eutopic endometrium and in endometrium from normal controls. These data suggest that in normal and eutopic endometrium, relaxin may exert a protective effect against endometriosi...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2010.06.051
更新日期：2010-12-01 00:00:00
abstract：:Hormone patterns during 113 nonconceptual gonadotropin-induced cycles of 65 infertile anovulatory women were analyzed. All but one women ovulated, i.e., the ovulation rate was 98%. Signs of defective corpus luteum function were observed during 8 cycles, and anovulation occurred in 11 treatment cycles. The duration of ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)47115-3
更新日期：1983-06-01 00:00:00
abstract：OBJECTIVE:To investigate the use of medroxyprogesterone acetate (MPA) to prevent LH surge during controlled ovarian hyperstimulation (COH) and to compare cycle characteristics and pregnancy outcomes in subsequently frozen-thawed ET (FET) cycles. DESIGN:A prospective controlled study. SETTING:Tertiary-care academic me...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2015.03.022
更新日期：2015-07-01 00:00:00
abstract：:Noninvasive imaging techniques play an important role in the female infertility diagnostic algorithm. In this review article, a description of the diagnostic performance and potential clinical value of both computerized tomographic virtual hysterosalpingography (CT-VHGS) and magnetic-resonance virtual hysterosalpingog...
journal_title：Fertility and sterility
pub_type： 杂志文章,评审
doi：10.1016/j.fertnstert.2016.04.016
更新日期：2016-06-01 00:00:00
abstract：OBJECTIVE:To compare the mRNA expression of vascular endothelial growth factor (VEGF) 121 and 165 isoforms and thrombospondin-1 (TSP-1) after raloxifene, 17beta-E(2), and P administration in cultured Ishikawa cells. DESIGN:Prospective basic research study. SETTING:Jones Institute for Reproductive Medicine, Eastern Vi...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(03)00350-9
更新日期：2003-06-01 00:00:00
abstract：:Eighty-seven patients who underwent a late secretory phase endometrial biopsy while taking clomiphene citrate (CC) for ovulation induction were studied. Of the endometrial biopsies, 21 (24%) showed an endometrium greater than 2 days out of phase (OOP) with respect to the subsequent menstrual cycle. All 87 patients wer...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)60726-4
更新日期：1989-06-01 00:00:00
abstract：:Danazol and gestrinone are effective drugs in the treatment of endometriosis. Their mechanism of action remains uncertain, but may be related to their androgenic activity. The authors examined the effect of danazol on human endometrial cells cultured in vitro, its two major metabolites, ethisterone and 2 hydroxymethyl...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)59706-4
更新日期：1988-02-01 00:00:00
abstract：:Varicocelectomy has been found to improve the spermogram in many subfertile men. However, there still remains a sizable group of subfertile men whose counts fail to improve sufficiently following surgery. Clomiphene citrate, which has been shown to improve fertility in idiopathic oligospermia, was similarly tried in m...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)44661-3
更新日期：1980-04-01 00:00:00
abstract：:A total of 82 patients underwent 106 cycles in which immature oocytes were recovered after hCG priming and transferred at blastocyst-stage. After transfer, the implantation rate was 26.8%, and the clinical pregnancy rate was 51.9%. We found that the oocytes retrieved after hCG priming in women with high risk of ovaria...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2006.11.027
更新日期：2007-06-01 00:00:00
abstract：OBJECTIVE:To evaluate whether serum replacement with growth factor-free synthetic substances contributed to the effective establishment of embryonic stem (ES) cells. DESIGN:Randomized, prospective model study. SETTING:Gamete and stem cell biotechnology laboratory at Seoul National University in Korea. ANIMAL(S):F1 (...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2006.01.056
更新日期：2006-10-01 00:00:00
abstract：OBJECTIVE:To evaluate the usefulness of narrow-band imaging (NBI) technology for improving the diagnostic reliability of hysteroscopy. DESIGN:Prospective controlled clinical study (Canadian Task Force classification II-2). SETTING:University hospital. PATIENT(S):Three hundred ninety-five outpatient women undergoing ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2009.12.083
更新日期：2010-11-01 00:00:00
abstract：:Klemetti's birth registry study is put in context of other major studies. Summary advice is offered to practitioners as to what they should tell parents who conceive with assisted reproductive technology. ...
journal_title：Fertility and sterility
pub_type： 评论,信件
doi：10.1016/j.fertnstert.2005.04.067
更新日期：2005-11-01 00:00:00
abstract：OBJECTIVE:To assess the prevalence of infectious complications and the protective effect of prophylactic antibiotic treatment after diagnostic office hysteroscopy in asymptomatic, infertile patients with normal results from transvaginal sonography. DESIGN:Recording of infectious complications after routine hysteroscop...
journal_title：Fertility and sterility
pub_type： 杂志文章,多中心研究,随机对照试验
doi：10.1016/j.fertnstert.2010.08.031
更新日期：2011-02-01 00:00:00
abstract：:In this prospective, follow-up study of 102 high-risk oocyte donors in their luteal phase, we found a complete absence of ovarian hyperstimulation syndrome (no signs of hemoconcentration or ascites) after the donors were triggered with a gonadotropin releasing-hormone (GnRH) agonist. Due to its powerful preventive eff...
journal_title：Fertility and sterility
pub_type： 临床试验,杂志文章
doi：10.1016/j.fertnstert.2009.08.036
更新日期：2010-05-01 00:00:00
abstract：:Tissue microarrays (TMAs) are useful tools for studying protein expression in endometriosis. Tissue microarray analyses of immunohistochemical profiles of estrogen receptor-alpha and P receptor corroborate previously published results from the use of conventional immunohistochemistry, thus validating TMA use in endome...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2004.06.044
更新日期：2004-12-01 00:00:00
abstract：OBJECTIVE:To compare oocyte maturation, fertilization and cleavage rates, and embryonic developmental quality after culture of human immature oocytes from polycystic ovary syndrome (PCOS) patients in human tubal fluid (HTF) or tissue culture medium (TCM) 199. DESIGN:Prospective, randomized, controlled trial. SETTING:...
journal_title：Fertility and sterility
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.fertnstert.2007.11.082
更新日期：2009-02-01 00:00:00
abstract：OBJECTIVE:To evaluate whether endometrioma is associated with a progressive decline in ovarian reserve, and to compare the rate of decline with natural decline in ovarian reserve. DESIGN:Prospective, observational study. SETTING:Tertiary university hospital, endometriosis clinic. PATIENT(S):Forty women with endometr...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2018.03.015
更新日期：2018-07-01 00:00:00
abstract：OBJECTIVE:To assess the association between transsexualism and allele and genotype frequencies of the common cytochrome P450 (CYP) 17 -34 T>C single nucleotide polymorphism (SNP). DESIGN:Case-control study. SETTING:Academic research institution. PATIENT(S):102 male-to-female (MtF) and 49 female-to-male (FtM) transse...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2007.05.056
更新日期：2008-07-01 00:00:00
abstract：OBJECTIVE:To evaluate whether adolescent obesity is associated with difficulties in becoming pregnant later in life. DESIGN:Cross-sectional analysis of baseline data from a longitudinal cohort. SETTING:Multiethnic, community-based observational study of U.S. women. PATIENT(S):Three thousand one hundred fifty-four mi...
journal_title：Fertility and sterility
pub_type： 杂志文章,多中心研究
doi：10.1016/j.fertnstert.2008.12.059
更新日期：2010-04-01 00:00:00
abstract：OBJECTIVE:To explore the contribution of an altered structure of sperm mitochondria to human asthenozoospermia. DESIGN:A retrospective study. SETTING:Andrology Clinic, University of L'Aquila. PATIENT(S):Fifteen ejaculates with forward motility (FM) ≥ 50%, and 57 asthenozoospermic ejaculates (FM <50%, sperm vitality ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2010.07.1086
更新日期：2011-02-01 00:00:00
