Abstract:
:SUMMARY The genus Botrytis contains necrotrophic plant pathogens that have a wide host range (B. cinerea) or are specialized on a single host species, e.g. B. elliptica on lily. In this study, it was found that B. elliptica-induced cell death of lily displays hallmark features of animal programmed cell death or apoptosis including cytoplasmic shrinkage, nuclear DNA fragmentation and the accumulation of NO as well as H(2)O(2). A pharmacological approach showed that B. elliptica-induced cell death could be modulated by serine and cysteine protease inhibitors including one caspase inhibitor. Blocking phosphatase activity stimulated cell death and concomitant lesion formation, suggesting that B. elliptica-induced cell death is mediated by kinase/phosphatase pathways. Blocking Ca(2+) influx restricted cell death. Blocking steps of sphingolipid biosynthesis delayed lily cell death for several days. B. elliptica culture filtrate (CF) was able to induce lily cell death by means of secreted proteins. Induction of cell death is necessary and sufficient for pathogenicity and host specialization because prior infiltration of B. elliptica CF enabled subsequent infection of lily by the otherwise incompatible pathogens B. cinerea and B. tulipae. The secreted B. elliptica proteins also induced cell death in some but not all Arabidopsis accessions and mutants. Arabidopsis accessions that respond to infiltration of B. elliptica CF also display cell death symptoms upon inoculation with B. elliptica conidia.
journal_name
Mol Plant Patholjournal_title
Molecular plant pathologyauthors
VAN Baarlen P,Staats M,VAN Kan JAdoi
10.1111/j.1364-3703.2004.00253.xsubject
Has Abstractpub_date
2004-11-01 00:00:00pages
559-74issue
6eissn
1464-6722issn
1364-3703pii
MPP253journal_volume
5pub_type
杂志文章abstract::Because of their highly ordered structure, mature viroid RNA molecules are assumed to be resistant to degradation by RNA interference (RNAi). In this article, we report that transgenic tomato plants expressing a hairpin RNA (hpRNA) construct derived from Potato spindle tuber viroid (PSTVd) sequences exhibit resistance...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/j.1364-3703.2009.00546.x
更新日期:2009-07-01 00:00:00
abstract::Natural and synthetic elicitors have contributed significantly to the study of plant immunity. Pathogen-derived proteins and carbohydrates that bind to immune receptors, allow the fine dissection of certain defence pathways. Lipids of a different nature that act as defence elicitors, have also been studied, but their ...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12252
更新日期:2015-12-01 00:00:00
abstract::SUMMARY We describe the use of degenerate primers to amplify the core hrp/hrc region of P. syringae pv. phaseolicola NPS3121. 18 826 bp were amplified using long-range PCR, digested and shotgun sequenced. Sequence analysis finds a hypervariable region between the hrpU and hrpC operons, and indications of positive sele...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/j.1364-3703.2004.00212.x
更新日期:2004-03-01 00:00:00
abstract::Colletotrichum graminicola is a hemibiotrophic fungus that causes anthracnose leaf blight (ALB) and anthracnose stalk rot (ASR) in maize. Despite substantial economic losses caused by these diseases, the defence mechanisms against this pathogen remain poorly understood. Several hormones are suggested to aid in defence...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12924
更新日期:2020-05-01 00:00:00
abstract::The use of genetic resistance is considered to be the most effective and sustainable approach to the control of plant pathogens. Although most of the known natural resistance genes are monogenic dominant R genes that are predominant against fungi and bacteria, more and more recessive resistance genes against viruses h...
journal_title:Molecular plant pathology
pub_type: 杂志文章,评审
doi:10.1111/j.1364-3703.2012.00791.x
更新日期:2012-09-01 00:00:00
abstract::Previously, we have determined the nonhost-mediated recognition of the MfAvr4 and MfEcp2 effector proteins from the banana pathogen Mycosphaerella fijiensis in tomato, by the cognate Cf-4 and Cf-Ecp2 resistance proteins, respectively. These two resistance proteins could thus mediate resistance against M. fijiensis if ...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12104
更新日期:2014-06-01 00:00:00
abstract::SUMMARY The Bipolaris sorokiniana tolerant 1 (bst1) barley mutant is derived from fast neutron-irradiated seeds of wild-type Bowman(Rph3). The induced mutation was genetically localized to a position on chromosome 5HL distal to the centromere using amplified fragment length polymorphism markers. In addition, the defen...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/j.1364-3703.2009.00555.x
更新日期:2009-09-01 00:00:00
abstract::SUMMARY Pathogenicity islands (PAIs) were first described in uropathogenic E. coli. They are now defined as regions of DNA that contain virulence genes and are present in the genome of pathogenic strains, but absent from or only rarely present in non-pathogenic variants of the same or related strains. Other features i...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1046/j.1364-3703.2003.00187.x
更新日期:2003-09-01 00:00:00
abstract::SUMMARY The phytotoxin coronatine is a jasmonate mimic produced by Pseudomonas syringae pv. tomato (Pst). Coronatine acts as a virulence factor in Arabidopsis and mutants insensitive to coronatine are resistant to Pst and have higher levels of salicylic acid (SA). In this work we used the SA-deficient lines NahG and s...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/j.1364-3703.2004.00265.x
更新日期:2005-01-01 00:00:00
abstract:UNLABELLED:The Oomycete genus Aphanomyces houses plant and animal pathogens found in both terrestrial and aquatic habitats. Aphanomyces euteiches Drechs. causes seedling damping off and root rot diseases on many legumes. It is the most devastating pea (Pisum sativum) disease in several countries, causing up to 80% loss...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/j.1364-3703.2007.00413.x
更新日期:2007-09-01 00:00:00
abstract::A high-throughput RNA-mediated gene silencing system was developed for Cochliobolus sativus (anamorph: Bipolaris sorokiniana), the causal agent of spot blotch, common root rot and black point in barley and wheat. The green fluorescent protein gene (GFP) and the proteinaceous host-selective toxin gene (ToxA) were first...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/j.1364-3703.2010.00666.x
更新日期:2011-04-01 00:00:00
abstract::In plants, RNA silencing is a surveillance mechanism against invading viruses. It involves the production of virus-derived small interfering RNAs (vsiRNAs), which guide the RNA-induced silencing complex (RISC) to inactivate viruses. vsiRNAs may also promote the silencing of host mRNAs in a sequence-specific manner. In...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/j.1364-3703.2012.00828.x
更新日期:2013-01-01 00:00:00
abstract::RNA-dependent RNA polymerase 1 (RDR1) plays a crucial role in plant defence against viruses. In this study, it was observed that cucumber, Cucumis sativus, uniquely encodes a small gene family of four RDR1 genes. The cucumber RDR1 genes (CsRDR1a, CsRDR1b and duplicated CsRDR1c1/c2) shared 55%-60% homology in their enc...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12518
更新日期:2018-02-01 00:00:00
abstract::SUMMARY Brachiaria is a pan-tropical genus of grasses with about 100 species. The fungus Acremonium implicatum can develop an endophytic association that is mutually beneficial with Brachiaria species. We developed a polymerase chain reaction (PCR)-based method by first amplifying DNA from A. implicatum isolates using...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1046/j.1364-3703.2003.00157.x
更新日期:2003-03-01 00:00:00
abstract::Plant pathology has a long-standing tradition of classifying microbes as pathogens, endophytes or saprophytes. Lifestyles of pathogens are categorized as biotrophic, necrotrophic or hemibiotrophic. Botrytis species are considered by many to be archetypal examples of necrotrophic fungi, with B. cinerea being the most e...
journal_title:Molecular plant pathology
pub_type: 杂志文章,评审
doi:10.1111/mpp.12148
更新日期:2014-12-01 00:00:00
abstract::Plants have evolved powerful immune systems to recognize pathogens and avoid invasions, but the genetic basis of plant susceptibility is less well-studied, especially to oomycetes, which cause disastrous diseases in many ornamental plants and food crops. In this research, we identified a negative regulator of plant im...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12883
更新日期:2020-01-01 00:00:00
abstract::Protein kinases have been implicated in the regulation of many processes that guide pathogen development throughout the course of infection. A survey of the Sclerotinia sclerotiorum genome for genes encoding proteins containing the highly conserved eukaryotic protein kinase (ePK) domain, the largest protein kinase sup...
journal_title:Molecular plant pathology
pub_type: 杂志文章,评审
doi:10.1111/mpp.12321
更新日期:2016-05-01 00:00:00
abstract::Several genes in the Agrobacterium tumefaciens transferred (T)-DNA encode proteins that are involved in developmental alterations, leading to the formation of tumours in infected plants. We investigated the role of the protein encoded by the Atu6002 gene, the function of which is completely unknown. Atu6002 expression...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12088
更新日期:2014-04-01 00:00:00
abstract::Abstract To test whether carbohydrates may play a signalling function during plant pathogenesis, we investigated the interaction between tobacco and potato virus Y (PVY(N)). Four days after PVY(N) infection, leaves started to accumulate soluble sugars and leaf photosynthesis decreased. The accumulation of soluble suga...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1046/j.1364-3703.2000.00007.x
更新日期:2000-01-01 00:00:00
abstract::Phytohormones, such as salicylic acid (SA), ethylene (ET) and jasmonic acid (JA), play key roles in plant defence following pathogen attack. The involvement of these hormones in susceptibility following Fusarium oxysporum (Fo) infection has mostly been studied in Arabidopsis thaliana. However, Fo causes vascular wilt ...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12559
更新日期:2017-09-01 00:00:00
abstract::Apple scab, caused by the fungal pathogen Venturia inaequalis, is one of the most severe diseases of apple worldwide. It is the most studied plant-pathogen interaction involving a woody species using modern genetic, genomic, proteomic and bioinformatic approaches in both species. Although 'Geneva' apple was recognized...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12269
更新日期:2016-02-01 00:00:00
abstract::The translocation of Melon necrotic spot virus (MNSV) within tissues of inoculated and systemically infected Cucumis melo L. 'Galia' was studied by tissue-printing and in situ hybridization techniques. The results were compatible with the phloem vascular components being used to spread MNSV systemically by the same as...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/j.1364-3703.2008.00474.x
更新日期:2008-07-01 00:00:00
abstract::Recently, Tomato spotted wilt virus (TSWV) nonstructural protein NSs has been identified unambiguously as an avirulence (Avr) determinant for Tomato spotted wilt (Tsw)-based resistance. The observation that NSs from two natural resistance-breaking isolates had lost RNA silencing suppressor (RSS) activity and Avr sugge...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12082
更新日期:2014-02-01 00:00:00
abstract::The single-stranded, positive-sense RNA genome of viruses in the genus Potyvirus encodes a large polyprotein that is cleaved to yield 10 mature proteins. The first three cleavage products are P1, HCpro and P3. An additional short open reading frame (ORF), called pipo, overlaps the P3 region of the polyprotein ORF. Fou...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12366
更新日期:2016-09-01 00:00:00
abstract::MicroRNAs (miRNAs) play essential roles in plant development. There is increasing evidence that changed expression of miRNAs in virus-infected plants contributes to the development of viral symptoms. Here, we analysed the altered expression of miRNAs of Nicotiana benthamiana in response to Potato virus X (PVX) by Illu...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12717
更新日期:2018-11-01 00:00:00
abstract::For several years, researchers working on the plant pathogen Botrytis cinerea and a number of other related fungi have routinely used the pLOB1 vector system, based on hygromycin resistance, under the control of the Aspergillus nidulans oliC promoter and what was reported to be the beta-tubulin (tubA) terminator. Rece...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/j.1364-3703.2010.00632.x
更新日期:2010-09-01 00:00:00
abstract::Sclerotinia sclerotiorum is a devastating necrotrophic fungal pathogen that infects over 400 species of plants worldwide. Reactive oxygen species (ROS) modulations are critical for the pathogenic development of S. sclerotiorum. The fungus applies enzymatic and non-enzymatic antioxidants to cope with the oxidative stre...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12801
更新日期:2019-07-01 00:00:00
abstract::SUMMARY The phytotoxin coronatine (COR) contributes to the virulence of Pseudomonas syringae pv. tomato (Pst) strain DC3000 on Arabidopsis thaliana and tomato. However, little is known regarding the role of COR in the virulence of DC3000 on cultivated Brassica spp. In this study, the role of COR and its precursors, co...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/j.1364-3703.2006.00372.x
更新日期:2007-01-01 00:00:00
abstract::Blue mould, caused primarily by Penicillium expansum, is a major threat to the global pome fruit industry, causing multimillion-dollar losses annually. The blue mould fungus negatively affects fruit quality, thereby reducing fresh fruit consumption, and significantly contributes to food loss. P. expansum also produces...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12990
更新日期:2020-11-01 00:00:00
abstract::In all fungi studied so far, mitogen-activated protein (MAP) kinase cascades serve as central signalling complexes that are involved in various aspects of growth, stress response and infection. In this work, putative components of the yeast Fus3/Kss1-type MAP kinase cascade and the putative downstream transcription fa...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/j.1364-3703.2009.00579.x
更新日期:2010-01-01 00:00:00