Abstract:
:Oligodendrocytes generate and maintain myelin, which is essential for axonal function and protection of the mammalian central nervous system. To advance our molecular understanding of differentiation by these cells, we screened libraries of pharmacologically active compounds and identified inducers of differentiation of Oli-neu, a stable cell line of mouse oligodendrocyte precursors (OPCs). We identified four broad classes of inducers, namely, forskolin/cAMP (protein kinase A activators), steroids (glucocorticoids and retinoic acid), ErbB2 inhibitors, and nucleoside analogs, and confirmed the activity of these compounds on rat primary oligodendrocyte precursors and mixed cortical cultures. We also analyzed transcriptional responses in the chemically induced mouse and rat OPC differentiation processes and compared these with earlier studies. We confirm the view that ErbB2 is a natural signaling component that is required for OPC proliferation, whereas ErbB2 inhibition or genetic knockdown results in OPC differentiation.
journal_name
J Neurosci Resjournal_title
Journal of neuroscience researchauthors
Joubert L,Foucault I,Sagot Y,Bernasconi L,Duval F,Alliod C,Frossard MJ,Pescini Gobert R,Curchod ML,Salvat C,Nichols A,Pouly S,Rommel C,Roach A,Hooft van Huijsduijnen Rdoi
10.1002/jnr.22434subject
Has Abstractpub_date
2010-09-01 00:00:00pages
2546-57issue
12eissn
0360-4012issn
1097-4547journal_volume
88pub_type
杂志文章abstract::Axonal injury is often characterized by axonal transport defects and abnormal accumulation of intra-axonal components. Nitric oxide (NO) has a key role in mediating inflammatory axonopathy in many neurodegenerative diseases, but little is known about how nitrosative/oxidative stress affects axonal transport or whether...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.23556
更新日期:2015-06-01 00:00:00
abstract::Calcium-activated neutral proteinase (CANP) activity was determined in cytosolic and membranous subcellular fractions of transformed Schwann cells (tSc). The muM and mM Ca(2+)-sensitive (mu- and mCANP) forms of CANP were separated by DEAE and phenyl Sepharose column chromatography, the latter step enabling removal of ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490290310
更新日期:1991-07-01 00:00:00
abstract::Reference genes are often used to normalize expression of data from real-time quantitative reverse transcription-polymerase chain reaction (RT-qPCR), and only a validation of their stability during a given experimental paradigm leads to reliable interpretations. The present study was thus designed to validate potentia...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.22282
更新日期:2010-04-01 00:00:00
abstract::Unilateral eye removal results in an increased delivery of blood-borne glucose, norepinephrine, choline, and tyrosine, but not of proline, to the denervated optic lobes. Administration of reserpine to chicks increases penetrance of norepinephrine into the brain but not of tyrosine, choline, or proline. Selective alter...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490010308
更新日期:1975-01-01 00:00:00
abstract::In the embryonic and postnatal rat retina a cell surface antigen that is detected by monoclonal antibody JONES is distributed in a dorsoventral gradient. Biochemical analysis has determined that the antigen is a modified ganglioside, 9-O-acetyl GD3. In the present study, the distributions of 9-O-acetyl GD3 and its pos...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490210231
更新日期:1988-10-01 00:00:00
abstract::Sky (also known as "Tyro3" and "Rse") is a member of the Axl/Sky/Mer receptor tyrosine kinase family and has two immunoglobulin-like repeats and two fibronectin type III-like repeats in the extracellular domain. Gas6 is a ligand for all members of the Axl family, each of which (Axl, Sky, and c-Mer) has different affin...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.10211
更新日期:2002-04-15 00:00:00
abstract::Protein zero (P0) glycoprotein is the major integral membrane protein of the peripheral nervous system myelin in higher vertebrates. Previous findings indicate the formation of tetrameric assemblies from studies on isolated P0. To determine whether in intact myelin the P0 exists as oligomers, we isolated myelin from s...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.10167
更新日期:2002-03-15 00:00:00
abstract::Calcium/calmodulin-dependent serine kinase (CASK), a causative gene in X-linked mental retardation, carries out multiple functions in neurons, including vesicle trafficking of ion channels, synapse formation, and gene transcription. From a yeast two-hybrid screen, Krüppel-like zinc finger protein B cell lymphoma/COUP-...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.22407
更新日期:2010-08-15 00:00:00
abstract::Mitotic activity persists in various regions of the adult mammal CNS. While evidences of neurogenesis appeared, many studies focused on the features of the adult stem cells from germinative areas such as the subventricular zone of the lateral ventricles, the dentate gyrus of the hippocampus, the cortex, the fourth ven...
journal_title:Journal of neuroscience research
pub_type: 杂志文章,评审
doi:10.1002/jnr.20040
更新日期:2004-04-15 00:00:00
abstract::It was previously reported that a monoclonal antibody to beta-amyloid precursor protein (mab22C11; Boehringer Mannheim, Indianapolis, IN) labels an intermediate filament-associated protein (beta APP-IFAP) in cultured human skin fibroblasts (Dooley et al.: J Neurosci Res 33:60-67, 1992). The time course of its expressi...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490380605
更新日期:1994-08-15 00:00:00
abstract::Nicotine has been reported to be neuroprotective in experimental and epidemiological studies. In addition to nicotine, tobacco and cigarette smoke contain cembranoids, which are antagonists of neuronal nicotinic receptors (nAChR). Exposure of hippocampal slices to N-methyl-D-aspartate (NMDA) decreases the population s...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.20666
更新日期:2005-12-01 00:00:00
abstract::The induction of heat-shock protein 70 (hsp70) mRNA in the hyperthermic rabbit brain has been examined previously by using Northern blotting and in situ hybridization procedures that measure steady-state levels of mRNA, which may be influenced by transcript stability and transcription rate. In the present investigatio...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4547(19980601)52:5<538::AID-JNR
更新日期:1998-06-01 00:00:00
abstract::The effects of hyposmotic conditions on taurine uptake and release were studied in mice cultured cerebellar granule cells. The effect of DIDS (4,4'-diisothiocyanatostilbene-2,2'-disulfonate) and of the divalent cations Mg++ and Mn++ on the hyposmolarity-induced changes in these parameters was investigated. Mg++ (20 mM...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490300409
更新日期:1991-12-01 00:00:00
abstract::Data from a study in which 12 chronic mental patients and 12 control subject were given D-[3-14C] glucose intravenously in our arterio-venous technique for determining cerebral metabolism in vivo were reexamined. Previously unpublished whole-blood lactate determinations in these experiments indicated a cerebral produc...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490060209
更新日期:1981-01-01 00:00:00
abstract::The ability of altered environmental conditions to modulate some properties of synaptosomes has been studied. Incubation conditions used included the presence of methyl mercury or an organochlorine insecticide: chlordecone. Other adverse chemical conditions during incubation were the absence of calcium salts from the ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490250211
更新日期:1990-02-01 00:00:00
abstract::Thymocyte differentiation antigen-1 (Thy-1) is a cell surface glycoprotein found on T cells and neurons and is involved in cell-to-cell interactions. In addition, Thy-1 knockouts (KO) are a potential mouse model of restless legs syndrome (RLS) based on clinical observations and the role of dopamine in the disease. In ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.23046
更新日期:2012-08-01 00:00:00
abstract::α-Synuclein (α-syn) is the major component of pathological inclusions characteristic of several neurodegenerative disorders, such as Parkinson's disease. The major posttranslational modification of α-syn is phosphorylation at S129, and previous studies estimate that approximately 90% of α-syn in proteinaceous, patholo...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.22537
更新日期:2011-02-01 00:00:00
abstract::Taurine, 2-aminoethanesulfonic acid, is present at high concentrations in many invertebrate and vertebrate systems and has several biological functions. In addition, it has been related to a neuroprotective role against several diseases such as epilepsy. In the present work, we treated mice with taurine and examined i...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.22192
更新日期:2010-01-01 00:00:00
abstract::HAND2 (also known as dHAND) is a basic helix-loop-helix (bHLH) transcription factor essential for development of the heart, limbs, and neural crest-derived lineages. HAND2 expression is observed in a number of tissues derived from the neural crest, including components of the peripheral nervous system, where it has be...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.20105
更新日期:2004-06-01 00:00:00
abstract::In the present study, following previous experience with electrolytic lesion of the rat brain, and subsequent reduction of reactive gliosis with 7 beta-hydroxycholesterol derivatives (Bochelen et al.: Neuroscience 51:827-834, 1992), we have performed a hemisection of the spinal cord in adult rats and investigated the ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490410110
更新日期:1995-05-01 00:00:00
abstract::The inherited deficiency of galactosylceramide beta-galactosidase (E.C. 3.2.1.46: galactocerebrosidase) activity results in globoid cell leukodystrophy in humans (Krabbe disease) and in mice (twitcher mutant). To determine whether Krabbe patients' cells complement twitcher cells to produce, in hybrid combination, grea...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490270406
更新日期:1990-12-01 00:00:00
abstract::Cisplatin is a highly effective chemotherapeutic agent but with significant ototoxic side effects. Apoptosis is an important mechanism of cochlear hair cell loss following exposure to an ototoxic level of cisplatin. The present study investigated the effects of the cannabinoid receptor 2 (CB2) ligand JWH-015 on cispla...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.21168
更新日期:2007-03-01 00:00:00
abstract::A cholinergically disrupted laboratory animal has been produced by administration of the cholinotoxin ethylcholine aziridinium mustard (AF64A), which produced a dysfunction in the cholinergic forebrain system. After AF64A treatment, a reduction of choline acetyl transferase (ChAT) activity was measured in the hippocam...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4547(19990115)55:2<178::AID-JNR
更新日期:1999-01-15 00:00:00
abstract::The purpose of this study was to determine if calpain-induced proteolysis was associated with retinal degeneration or dysfunction in the rat acute ocular hypertensive model. Acute glaucoma was produced by elevation of IOP to 120 mm Hg for 1 hr. Retinal degeneration was evaluated by H&E staining and apoptosis was deter...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.20827
更新日期:2006-05-15 00:00:00
abstract::The combination of nerve growth factor (NGF) and phorbol 12-myristate 13-acetate (PMA) rapidly induced the locomotion of PC12 cells by sequentially stimulating lamellar spreading, ruffling with pinocytosis, and polarization by retraction from the substratum. During migration, cells acquired long processes as a result ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4547(19960515)44:4<308::AID-JNR
更新日期:1996-05-15 00:00:00
abstract::Mutations in the beta-amyloid precursor protein (APP) gene cause familial Alzheimer's disease (AD). Although amyloid beta peptide (Abeta) is the principal constituent of senile plaques in AD, other cleavage products of APP are also implicated in playing a role in the pathogenesis of AD. C-terminal fragments of APP (AP...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4547(20000515)60:4<565::AID-JNR
更新日期:2000-05-15 00:00:00
abstract::Ten days after bilateral injections of ibotenic acid into the nucleus basalis, rats injected daily (i.m.) with ganglioside GM1 were protected from anterograde degeneration of cholinergic projections to the fronto-lateral cortex. This protection was reflected by reduced losses (associated with ibotenic acid lesions) of...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490200411
更新日期:1988-08-01 00:00:00
abstract::Efficient functioning of maintenance and repair processes seem to be crucial for both survival and physical quality of life. This is accomplished by a complex network of the so-called longevity assurance processes, under control of several genes termed vitagenes. These include members of the heat shock protein system,...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.20386
更新日期:2005-02-15 00:00:00
abstract::The effect of neonatal hyperthyroidism produced by injection of tri-iodothyronine (T3) on myelination and on the microperoxisomal population of the brain was studied in young rats. Data on the lipid composition of myelin show that myelinogenesis starts earlier in treated animals. In coincidence with the early appearan...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490250312
更新日期:1990-03-01 00:00:00
abstract::Rapsyn is a protein that interacts with the cytoplasmic face of the nicotinic acetylcholine receptors (AChR) to cluster them within postsynaptic membrane of muscle. Here we show that intracellular AChRs are also affected by rapsyn. When rapsyn was co-transfected with AChR into QT-6 fibroblasts, (125)I-alpha-bungarotox...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4547(20000415)60:2<155::AID-JNR
更新日期:2000-04-15 00:00:00