Abstract:
BACKGROUND:The new swine influenza (H1N1) requires fast and accurate diagnosis. Currently, there are few reports about the diagnostic performance of influenza antigen tests with regard to the H1N1 virus infection. We evaluated the reactivity of eight commercially available rapid antigen tests in samples from confirmed infected patients. METHODS:From 23 patients tested positive by polymerase chain reaction (PCR), parallel testing with the antigen tests QuickVue Influenza A+B (Quidel Corporation), Binax NOW Flu A and B (Binax), Clearview Exact Influenza A and B (Inverness medical), TRUFLU (Meridian Bioscience), Directigen EZ Flu A+B (Becton Dickinson), Influenza A and B (Diagnostik Nord), Xpect Flu A and B (remel) and Influenza Antigen Quick (Generic Assays) was performed. RESULTS:Only few antigen tests showed positive reactivity in six of the 23 samples. The virus load of the reactive samples as indicated by the PCR cycle threshold was high. Negative results of rapid influenza tests do not rule out infection with the new influenza A (H1N1) virus. Their analytical sensitivity with reference to the virus load is low and not reproducible. CONCLUSIONS:As reliable laboratory results during a pandemic are essential, the diagnostic use of rapid influenza tests seems to be questionable.
journal_name
Clin Chem Lab Medjournal_title
Clinical chemistry and laboratory medicineauthors
Herzum I,Lutz T,Koch F,Geisel R,Gehrt Adoi
10.1515/CCLM.2010.022subject
Has Abstractpub_date
2010-01-01 00:00:00pages
53-6issue
1eissn
1434-6621issn
1437-4331journal_volume
48pub_type
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journal_title:Clinical chemistry and laboratory medicine
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doi:10.1515/CCLM.1998.133
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