Pancreatic phospholipase A2 activity in acute pancreatitis: a prognostic marker for early identification of patients at risk.

Abstract:

:Remarkably elevated levels of phospholipase A2 (PLA2) are measurable in human blood samples in cases of acute pancreatitis. The source of the enzyme was first thought to be exclusively the pancreas, but now it is generally accepted that two isoenzymes--the pancreatic PLA2, group I, and the extrapancreatic PLA2, group II--contribute to the raised activity. In contrast to the group II-PLA2, the pancreatic PLA2 is heat-resistant for 1 hour at 60 degrees C. The catalytically inactive proenzyme of the pancreatic PLA2 can be activated by trypsin. The aim of our study was to evaluate the diagnostic value of PLA2 isoenzyme activity measurements to identify patients with severe complications in acute pancreatitis. Blood samples from patients suffering from acute pancreatitis were analyzed for catalytically active pancreatic PLA2 on day 1 and 2 of hospitalization with a modified radiometric Escherichia coli-based PLA2 assay. In 10 of 41 patients clearly elevated values of catalytically active, heat-resistant pancreatic PLA2 (7.2 to 81.2 U/l) were observed. This group of patients was characterized by severe complications (necrotizing pancreatitis, shock, sepsis, respiratory problems) of which two patients subsequently died. Patients with low or undetectable activity (<7 U/l) of pancreatic PLA2 recovered rapidly. According to these results the presence of catalytically active pancreatic PLA2 in serum is associated with severe complications of acute pancreatitis. In contrast to total serum-PLA2, the catalytic concentration of pancreatic PLA2 can serve as a prognostic marker in acute pancreatitis.

journal_name

Clin Chem Lab Med

authors

Aufenanger J,Samman M,Quintel M,Fassbender K,Zimmer W,Bertsch T

doi

10.1515/CCLM.2002.046

keywords:

subject

Has Abstract

pub_date

2002-03-01 00:00:00

pages

293-7

issue

3

eissn

1434-6621

issn

1437-4331

journal_volume

40

pub_type

杂志文章
  • Pancreatic cancer biomarkers discovery by surface-enhanced laser desorption and ionization time-of-flight mass spectrometry.

    abstract:BACKGROUND:Surface-enhanced laser desorption and ionization time-of-flight mass spectrometry (SELDI-TOF/MS), a laboratory-friendly technique, is used to identify biomarkers for cancer. The aim of the present study was to explore the application of SELDI proteomic patterns in serum for distinguishing between cases of pa...

    journal_title:Clinical chemistry and laboratory medicine

    pub_type: 杂志文章

    doi:10.1515/CCLM.2009.158

    authors: Navaglia F,Fogar P,Basso D,Greco E,Padoan A,Tonidandel L,Fadi E,Zambon CF,Bozzato D,Moz S,Seraglia R,Pedrazzoli S,Plebani M

    更新日期:2009-01-01 00:00:00

  • Cardiac biomarkers and risk assessment in patients undergoing major non-cardiac surgery: time to revise the guidelines?

    abstract::Although the perioperative event rate has declined over the past 30 years, as a consequence of the developments in anesthesiology and surgical techniques, perioperative cardiovascular complications are still a significant clinical problem. At the end of the last century, a pooled analysis of several large studies foun...

    journal_title:Clinical chemistry and laboratory medicine

    pub_type: 杂志文章

    doi:10.1515/cclm-2013-0900

    authors: Clerico A,Emdin M,Passino C

    更新日期:2014-07-01 00:00:00

  • Serum chitotriosidase enzyme activity in patients with Crimean-Congo hemorrhagic fever.

    abstract:BACKGROUND:Crimean-Congo hemorrhagic fever (CCHF) is a public health problem in many countries. Chitotriosidase (ChT) is an enzyme secreted by activated macrophages that catalyzes the hydrolysis of chitin and chitin-like substrates. The goal of this study was to assess the relationship between serum ChT activity and mo...

    journal_title:Clinical chemistry and laboratory medicine

    pub_type: 杂志文章

    doi:10.1515/CCLM.2009.347

    authors: Kurt YG,Cayci T,Onguru P,Akgul EO,Yaman H,Aydin I,Bodur H,Turker T,Kurt I,Cevik MA,Erbil MK

    更新日期:2009-01-01 00:00:00

  • Anti-N-homocysteinylated protein autoantibodies and cardiovascular disease.

    abstract::Metabolic conversion of homocysteine (Hcy) to a chemically reactive metabolite, Hcy-thiolactone, catalyzed by methionyl-tRNA synthetase is the first step in a pathway that is suggested to contribute to Hcy toxicity in humans. The accumulation of Hcy-thiolactone is detrimental because of its intrinsic ability to modify...

    journal_title:Clinical chemistry and laboratory medicine

    pub_type: 杂志文章,评审

    doi:10.1515/CCLM.2005.177

    authors: Jakubowski H

    更新日期:2005-01-01 00:00:00

  • Clinical interpretation of reference intervals and reference limits. A plea for assay harmonization.

    abstract::Reference intervals for healthy subjects and diseased populations are important benchmarks for the clinical interpretation of laboratory test values. It is important that the testing conditions used to collect the reference data be closely matched with the testing conditions used for patient data. Interlaboratory diff...

    journal_title:Clinical chemistry and laboratory medicine

    pub_type: 杂志文章,评审

    doi:10.1515/CCLM.2004.127

    authors: Klee GG

    更新日期:2004-01-01 00:00:00

  • Standardization of DiaSorin and Roche automated third generation PTH assays with an International Standard: impact on clinical populations.

    abstract:BACKGROUND:Standardization of parathyroid hormone (PTH) assays is a major issue, especially in hemodialyzed (HD) patients. Two automated third generation PTH assays (Roche Elecsys and DiaSorin Liaison) are now available. These assays are specific for the (1-84) PTH and do not cross-react with the (7-84) fragment, contr...

    journal_title:Clinical chemistry and laboratory medicine

    pub_type: 杂志文章

    doi:10.1515/cclm-2013-1027

    authors: Cavalier E,Delanaye P,Lukas P,Carlisi A,Gadisseur R,Souberbielle JC

    更新日期:2014-08-01 00:00:00

  • Investigation on the ability of first trimester glycodelin and angiopoietin-2 to predict small-for-gestational age pregnancies at delivery.

    abstract:BACKGROUND:The aim was to investigate whether first trimester glycodelin and angiopoietin-2 can predict small-for-gestational age (SGA) at delivery, individually or in combination. METHODS:In this case-control study we measured glycodelin and angiopoietin-2 on serum from 170 singleton pregnant women delivering SGA neo...

    journal_title:Clinical chemistry and laboratory medicine

    pub_type: 杂志文章

    doi:10.1515/cclm-2015-0989

    authors: Hansen YB,Myrhøj V,Jørgensen FS,Sørensen S

    更新日期:2016-09-01 00:00:00

  • The association between plasminogen activator inhibitor type 1 (PAI-1) levels, PAI-1 4G/5G polymorphism, and myocardial infarction: a Mendelian randomization meta-analysis.

    abstract:BACKGROUND:The circulating levels of plasminogen activator inhibitor type 1 (PAI-1) are increased in individuals carrying the 4G allele at position -675 of the PAI-1 gene. In turn, overexpression of PAI-1 has been found to affect both atheroma and thrombosis. However, the association between PAI-1 levels and the incide...

    journal_title:Clinical chemistry and laboratory medicine

    pub_type: 杂志文章,meta分析

    doi:10.1515/cclm-2013-1124

    authors: Nikolopoulos GK,Bagos PG,Tsangaris I,Tsiara CG,Kopterides P,Vaiopoulos A,Kapsimali V,Bonovas S,Tsantes AE

    更新日期:2014-07-01 00:00:00

  • Research translation: a new frontier for clinical laboratories.

    abstract::Translational research and translational medicine (referred to hereafter as translational research) are interchangeable terms that underline the pressing need to translate into practical benefits for those affected by disease the extensive investments divested by the private and public sectors in biomedical research. ...

    journal_title:Clinical chemistry and laboratory medicine

    pub_type: 杂志文章,评审

    doi:10.1515/CCLM.2006.238

    authors: Plebani M,Marincola FM

    更新日期:2006-01-01 00:00:00

  • Decreased serum brain-derived neurotrophic factor (BDNF) is associated with post-stroke depression but not with BDNF gene Val66Met polymorphism.

    abstract:BACKGROUND:Decreased brain-derived neurotrophic factor (BDNF) has been demonstrated in animal models and patients with depression. However, little is known about changes in BDNF in post-stroke depression (PSD). This study investigated the changes in serum BDNF in patients with PSD, and evaluated whether serum concentra...

    journal_title:Clinical chemistry and laboratory medicine

    pub_type: 杂志文章

    doi:10.1515/CCLM.2011.039

    authors: Zhou Z,Lu T,Xu G,Yue X,Zhu W,Ma M,Liu W,Zhu S,Liu X

    更新日期:2011-02-01 00:00:00

  • Phospholipases A2 in gastric juice of Helicobacter pylori--positive and negative individuals.

    abstract::Gastric juice is known to have phospholipase A2 catalytic activity. Helicobacter pylori (H. pylori) has been reported to produce phospholipase A2, which is believed to hydrolyse the protective layer of gastric mucosal phospholipids and to promote mucosal damage. The current study aimed at identifying secretory phospho...

    journal_title:Clinical chemistry and laboratory medicine

    pub_type: 临床试验,杂志文章

    doi:10.1515/CCLM.1999.009

    authors: Huhtinen HT,Grönroos JM,Haapamäki MM,Nevalainen TJ

    更新日期:1999-01-01 00:00:00

  • Influence of the needle bore size used for collecting venous blood samples on routine clinical chemistry testing.

    abstract:BACKGROUND:Despite remarkable advances in technology and laboratory automation, results of laboratory testing still suffer from a high degree of preanalytical variability. Although there is no definitive evidence, the use of small-gauge needles for venipuncture is usually discouraged to reduce the chance of producing u...

    journal_title:Clinical chemistry and laboratory medicine

    pub_type: 杂志文章

    doi:10.1515/CCLM.2006.172

    authors: Lippi G,Salvagno GL,Montagnana M,Brocco G,Cesare Guidi G

    更新日期:2006-01-01 00:00:00

  • Certification of reference materials for detection of the human prothrombin gene G20210A sequence variant.

    abstract:BACKGROUND:There is a need for reference materials (RMs) in the field of genetic testing for verification of test results obtained in patients and probands. For the frequent genetic variation G20210A in the prothrombin gene, it has been shown that purified plasmids containing the gene fragment harbouring the mutation c...

    journal_title:Clinical chemistry and laboratory medicine

    pub_type: 杂志文章

    doi:10.1515/CCLM.2008.093

    authors: Gancberg D,Corbisier P,Meeus N,Marki-Zay J,Mannhalter C,Schimmel H

    更新日期:2008-01-01 00:00:00

  • Advantages and limitations of total laboratory automation: a personal overview.

    abstract::Automation is considered one of the most important breakthroughs in the recent history of laboratory diagnostics. In a model of total laboratory automation (TLA), many analyzers performing different types of tests on different sample matrices are physically integrated as modular systems or physically connected by asse...

    journal_title:Clinical chemistry and laboratory medicine

    pub_type: 杂志文章

    doi:10.1515/cclm-2018-1323

    authors: Lippi G,Da Rin G

    更新日期:2019-05-27 00:00:00

  • Elecsys® and Kryptor immunoassays for the measurement of sFlt-1 and PlGF to aid preeclampsia diagnosis: are they comparable?

    abstract::Background For pregnant women with suspected preeclampsia, the soluble fms-like tyrosine-kinase 1 (sFlt-1)/placental growth factor (PlGF) ratio is a biomarker to aid diagnosis. We performed method comparisons between Elecsys® and Kryptor sFlt-1 and PlGF immunoassays and assessed the diagnostic performance for preeclam...

    journal_title:Clinical chemistry and laboratory medicine

    pub_type: 杂志文章

    doi:10.1515/cclm-2018-1228

    authors: Stepan H,Hund M,Dilba P,Sillman J,Schlembach D

    更新日期:2019-08-27 00:00:00

  • Using multiples of the median to normalize serum protein measurements.

    abstract::Quantification of serum proteins is useful in the diagnosis and clinical management of many disorders. With the introduction of automated analyzers and standardized reference materials, one of the last barriers to more widespread utilization of these measurements is the lack of availability of reliable and transferabl...

    journal_title:Clinical chemistry and laboratory medicine

    pub_type: 杂志文章

    doi:10.1515/CCLM.2001.180

    authors: Palomaki GE,Neveux LM

    更新日期:2001-11-01 00:00:00

  • Nitration of human plasminogen by RAW 264.7 macrophages reduces streptokinase-induced plasmin activity.

    abstract::Despite many years of study, clinical trials of new drugs to prevent thrombosis have often been disappointing. Part of the problem lies in our incomplete understanding of the regulation of plasminogen activation and/or inhibition in vivo. We have previously shown that in vitro nitration of plasminogen in plasma by per...

    journal_title:Clinical chemistry and laboratory medicine

    pub_type: 杂志文章

    doi:10.1515/CCLM.2006.039

    authors: Hathuc C,Hermo R,Schulze J,Gugliucci A

    更新日期:2006-01-01 00:00:00

  • Testing for HbA1c, in addition to the oral glucose tolerance test, in screening for abnormal glucose regulation helps to reveal patients with early β-cell function impairment.

    abstract:BACKGROUND:The oral glucose tolerance test (OGTT) is recommended to screen for diabetes in patients with coronary artery disease. We hypothesized that testing for glycated hemoglobin (HbA1c), in addition to the OGTT, in screening for abnormal glucose regulation may help to reveal patients with β-cell function impairmen...

    journal_title:Clinical chemistry and laboratory medicine

    pub_type: 杂志文章

    doi:10.1515/cclm-2017-0846

    authors: Li YH,Sheu WH,Lee WJ,Lee IT,Lin SY,Lee WL,Liang KW,Wang JS

    更新日期:2018-07-26 00:00:00

  • Mutation screening of the medium-chain acyl-CoA dehydrogenase (MCAD) and the ornithine transcarbamylase (OTC) genes by multiplex PCR amplification and sequencing.

    abstract:BACKGROUND:Sequencing based mutation screening assays of genes encompassing large numbers of exons could be substantially optimized by multiplex PCR, which enables simultaneous amplification of many targets in one reaction. In the present study, a multiplex PCR protocol originally developed for fragment analysis was ev...

    journal_title:Clinical chemistry and laboratory medicine

    pub_type: 杂志文章

    doi:10.1515/CCLM.2009.012

    authors: Horn MP,Mäder-Heinemann G,Andrey G,Largiadèr CR

    更新日期:2009-01-01 00:00:00

  • HAND1 loss-of-function mutation associated with familial dilated cardiomyopathy.

    abstract:BACKGROUND:The basic helix-loop-helix transcription factor HAND1 is essential for cardiac development and structural remodeling, and mutations in HAND1 have been causally linked to various congenital heart diseases. However, whether genetically compromised HAND1 predisposes to dilated cardiomyopathy (DCM) in humans rem...

    journal_title:Clinical chemistry and laboratory medicine

    pub_type: 杂志文章

    doi:10.1515/cclm-2015-0766

    authors: Zhou YM,Dai XY,Qiu XB,Yuan F,Li RG,Xu YJ,Qu XK,Huang RT,Xue S,Yang YQ

    更新日期:2016-07-01 00:00:00

  • Lactase persistence genotyping: rapid detection of seven sequence variants in a single tube with melting curve analyses.

    abstract:BACKGROUND:Lactase persistence is an inherited autosomal dominant trait that confers the ability to digest lactose after weaning. Lactose persistence is caused by single nucleotide variants in a regulatory element for the lactase gene (LCT). In Europeans, lactase persistence is associated with the nucleotide variant LC...

    journal_title:Clinical chemistry and laboratory medicine

    pub_type: 杂志文章

    doi:10.1515/cclm-2014-0123

    authors: Strand H,Sørensen LK,Ingebretsen OC

    更新日期:2014-09-01 00:00:00

  • Bone marrow microvascular density and angiogenic growth factors in multiple myeloma.

    abstract::There is evidence that angiogenesis plays an important role in the progression of multiple myeloma (MM). Hepatocyte growth factor (HGF) and tumor necrosis factor-alpha (TNF-alpha) are cytokines that potently stimulate angiogenesis. We evaluated the microvascular density (MVD) of bone marrow biopsies (after immunostain...

    journal_title:Clinical chemistry and laboratory medicine

    pub_type: 杂志文章

    doi:10.1515/CCLM.2004.230

    authors: Alexandrakis MG,Passam FJ,Ganotakis E,Dafnis E,Dambaki C,Konsolas J,Kyriakou DS,Stathopoulos E

    更新日期:2004-01-01 00:00:00

  • Unexpected high plasma cobalamin : proposal for a diagnostic strategy.

    abstract::It is well-established that more than 8% of patients examined for vitamin B12 deficiency unexpectedly have increased plasma levels of the vitamin, but so far there are no guidelines for the clinical interpretation of such findings. In this review, we summarise known associations between high plasma cobalamin and disea...

    journal_title:Clinical chemistry and laboratory medicine

    pub_type: 杂志文章,评审

    doi:10.1515/cclm-2012-0545

    authors: Arendt JF,Nexo E

    更新日期:2013-03-01 00:00:00

  • Rule-out of non-ST elevation myocardial infarction by five point of care cardiac troponin assays according to the 0 h/3 h algorithm of the European Society of Cardiology.

    abstract:BACKGROUND:Point of care (POC) assays for cardiac troponins I or T (cTnI or cTnT) may accelerate the diagnosis of patients with suspected acute coronary syndrome (ACS). However, their clinical utility according to the 0 h/3 h algorithm recommended by the European Society of Cardiology (ESC) for non-ST elevation myocard...

    journal_title:Clinical chemistry and laboratory medicine

    pub_type: 杂志文章

    doi:10.1515/cclm-2017-0486

    authors: Suh D,Keller DI,Hof D,von Eckardstein A,Gawinecka J

    更新日期:2018-03-28 00:00:00

  • Hypophosphatemia, fever and prolonged length of hospital stay in seronegative PCR positive patients as compared to seropositive patients with early acute Q fever pneumonia.

    abstract:BACKGROUND:Query fever (Q fever) is a zoonotic infection, caused by the intracellular Gram-negative coccobacillus Coxiella burnetii. From 2007 until 2010, a large Q fever outbreak has occurred in the Netherlands. We studied traditional and less common inflammation markers in seronegative and seropositive patients with ...

    journal_title:Clinical chemistry and laboratory medicine

    pub_type: 杂志文章

    doi:10.1515/cclm-2011-0810

    authors: Vissers E,de Jager CP,Hilbink M,Kusters R,van Gageldonk-Lafeber AB,Wever PC

    更新日期:2012-02-04 00:00:00

  • Stability of brain natriuretic peptide (BNP) in human whole blood and plasma.

    abstract::Brain natriuretic peptide is proposed as a biochemical marker which could provide a useful screening test to select patients for further cardiac investigations in heart failure. The applicability of such a biochemical test in clinics, hospital wards, and clinical laboratories is dependent on its ease of use and on the...

    journal_title:Clinical chemistry and laboratory medicine

    pub_type: 杂志文章

    doi:10.1515/CCLM.2000.076

    authors: Gobinet-Georges A,Valli N,Filliatre H,Dubernet MF,Dedeystere O,Bordenave L

    更新日期:2000-06-01 00:00:00

  • Sandwich ELISAs for soluble immunoglobulin superfamily receptor translocation-associated 2 (IRTA2)/FcRH5 (CD307) proteins in human sera.

    abstract:BACKGROUND:The immunoglobulin superfamily receptor translocation-associated 2 (IRTA2) gene encodes both membrane and secreted forms of the B-cell differentiation antigen (also identified as FcRH5). The membrane form is highly expressed on the surface of hairy cell leukemia (HCL) cells from patients. This study aimed to...

    journal_title:Clinical chemistry and laboratory medicine

    pub_type: 杂志文章

    doi:10.1515/CCLM.2006.115

    authors: Ise T,Kreitman RJ,Pastan I,Nagata S

    更新日期:2006-01-01 00:00:00

  • Real-time RT-PCR for the measurement of prostate-specific antigen mRNA expression in benign hyperplasia and adenocarcinoma of prostate.

    abstract::Since PSA is supposed to play an active role in the progression of prostate cancer, we applied a quantitative RT-PCR to measure the absolute levels of prostate-specific antigen (PSA) mRNA expression in benign and malignant prostatic tissue. Consecutive fine needle prostate biopsy material from 59 patients (43 with pro...

    journal_title:Clinical chemistry and laboratory medicine

    pub_type: 杂志文章

    doi:10.1515/CCLM.2003.040

    authors: Gelmini S,Tricarico C,Petrone L,Forti G,Amorosi A,Dedola GL,Serio M,Pazzagli M,Orlando C

    更新日期:2003-03-01 00:00:00

  • Diagnosis of sphingolipidoses: a new simultaneous measurement of lysosphingolipids by LC-MS/MS.

    abstract:BACKGROUND:Lysosphingolipids (LysoSLs) are derivatives of sphingolipids which have lost the amide-linked acyl chain. More recently, LysoSLs have been identified as storage compounds in several sphingolipidoses, including Gaucher, Fabry and Niemann-Pick diseases. To date, different methods have been developed to measure...

    journal_title:Clinical chemistry and laboratory medicine

    pub_type: 杂志文章

    doi:10.1515/cclm-2016-0340

    authors: Polo G,Burlina AP,Kolamunnage TB,Zampieri M,Dionisi-Vici C,Strisciuglio P,Zaninotto M,Plebani M,Burlina AB

    更新日期:2017-03-01 00:00:00

  • Bovine spongiform encephalopathy: are the cows mad or full of carbohydrates?

    abstract::The non-forage feeding of dairy cows rich in fast absorption carbohydrates, the low value of their euglycemic-hyperinsulinemic clamp suggest a dysregulation of carbohydrate metabolism able to produce neurodegenerative disorders. Comparisons between Alzheimer's disease developed in diabetes mellitus and bovine spongifo...

    journal_title:Clinical chemistry and laboratory medicine

    pub_type: 杂志文章

    doi:10.1515/CCLM.2002.018

    authors: Frey J

    更新日期:2002-02-01 00:00:00