Abstract:
:In solid tumours, necrosis is commonly found in the core region in response to metabolic stress that results from oxygen and glucose depletion (OGD) due to insufficient vascularization and has been implicated in tumour progression. We have previously shown that metabolic stress due to glucose depletion (GD) induces necrosis and HMGB1 release through mitochondrial ROS production in A549 lung adenocarcinoma cells. In this study, we examined the effects of hypoxia on GD-induced necrosis and show that hypoxia prevented GD-induced mitochondrial ROS production, HMGB1 release, and necrosis and switched the cell death mode to apoptosis that is dependent on caspase-3 and -9. We further found that inhibition of ERK1/2 by U0126 abolished the effects of hypoxia to switch the cell death mode and to suppress mitochondrial ROS production, indicating an important role(s) of the ERK pathway in cell death mode determination. We also found that during OGD-induced apoptosis the prosurvival protein kinase Akt is activated and inhibition of Akt by the phosphoinositide 3-kinase (PI3K) inhibitors LY294002 and wortmannin prevent OGD-induced apoptosis, caspase-3 and -9 activation, and nuclear translocation of AIF and EndoG. Similar inhibitory effects of PI3K inhibitors were observed in A549 cells that underwent apoptosis when treated with GD in the presence of NAC (a general antioxidant) or catalase (a H(2)O(2) scavenger), or in the presence of active PKC by treatment with phorbol-12-myristate-13-acetate, indicating a crucial role(s) of the PI3K-Akt pathway in OGD-indcued apoptosis. In conclusion, our results demonstrate that hypoxia switches GD-induced necrosis to apoptosis and ERK1/2 and PI3K-Akt exert anti-necrotic and pro-apoptotic activities in the cell death, respectively.
journal_name
Int J Oncoljournal_title
International journal of oncologyauthors
Kim CH,Ko AR,Lee SY,Jeon HM,Kim SM,Park HG,Han SI,Kang HSsubject
Has Abstractpub_date
2010-01-01 00:00:00pages
117-24issue
1eissn
1019-6439issn
1791-2423journal_volume
36pub_type
杂志文章abstract::The aim of this study was to demonstrate the potential of electrogene therapy with the bacterial purine nucleoside phosphorylase gene (ePNP), on pancreatic carcinoma (PC) large tumors. The in vivo electroporation (EP) conditions and efficacy were investigated on both subcutaneous xenografts of human PC cells in immuno...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2007-06-01 00:00:00
abstract::Over the past few decades, there has been a tremendous increase in cancer biology data and treatment. Cancer research has opened exciting new areas of cellular and molecular biology. Month by month, new genes which regulate the carcinogenesis process are being discovered. The result is an incredible knowledge of cance...
journal_title:International journal of oncology
pub_type: 杂志文章,评审
doi:10.3892/ijo.15.6.1213
更新日期:1999-12-01 00:00:00
abstract::Long noncoding RNA (lncRNA) MAF BZIP transcription factor G antisense RNA 1 (MAFG‑AS1) has been demonstrated to serve an important role in the progression of various types of cancer, whereas its role in breast cancer has not been fully elucidated. The present study aimed to explore the potential role and underlying me...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2020.5150
更新日期:2020-11-20 00:00:00
abstract::Arsenic trioxide (As2O3) can induce clinical remission in patients with acute promyelocytic leukemia (APL) through induction of apoptosis. To investigate the potential therapeutic usage of As2O3 in cervical cancer and its possible mechanisms, human cervical cancer cell line HeLa was employed. The cells underwent apopt...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2002-07-01 00:00:00
abstract::We have established a new human alphafetoprotein (AFP)-producing gallbladder carcinoma (GBC) cell line, termed KMG-C, from a 67-year-old Japanese male. KMG-C and its reconstituted tumors in nude mice showed the morphological features of an adenocarcinoma. Functionally, KMG-C secreted AFP, carcinoembryonic antigen, and...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.8.6.1179
更新日期:1996-06-01 00:00:00
abstract::Nestin is an intermediate filament protein and a stem cell marker expressed in several tumours. There is growing evidence of an association between the expression level of nestin and the pathogenesis of triple-negative breast cancer (TNBC). Nestin is also expressed in newly forming tumour vessels and is a valuable mar...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2017.4223
更新日期:2018-02-01 00:00:00
abstract::We hypothesize that dysregulated expression levels of the developmental regulatory genes in the adult body result in tumor development and malignant progression. PAX genes discovered as human orthologous genes of Drosophila 'paired' encode transcription factors, which control the expression of target genes to go on al...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2008-11-01 00:00:00
abstract::Over-expression of p21Waf-1 was performed in 5 human glioma cell lines (U251MG, U87MG, T98G, NMC-G1, and KALS-1). p53 gene status was examined by PCR-SSCP and direct sequence method. The endogenous p21Waf-1 expression was evaluated by RT-PCR. Adenovirus-mediated over-expression of p21Waf-1 inhibited the proliferation ...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.14.1.63
更新日期:1999-01-01 00:00:00
abstract::Recently, the inflammatory cytokine IL-6 has been reported as a potent inducer of epithelial-mesenchymal transition (EMT) in breast cancer cells with an epithelial phenotype. Furthermore, EMT induces stem cell features in normal and transformed mammary cells. We explored whether IL-...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2011.1275
更新日期:2012-04-01 00:00:00
abstract::We have isolated a new -ene, named BAC, which is the human equivalent of the murine H19 gene and is highly expressed in most fetal tissues and in a variety of fresh tumors. BAC was analyzed in 130 untreated invasive carcinomas of different types. The frequency of BAC-expressing cancers as well as the level of expressi...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2.5.753
更新日期:1993-05-01 00:00:00
abstract::Wilms' tumor is one of the most common malignant tumors of the abdomen in children. However, there is currently no recognized specific biomarker for the clinical diagnosis and prognosis of this tumor. Lipid metabolism is involved in membrane synthesis and oxidation in tumor cells. This process plays an important role ...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2019.4948
更新日期:2020-02-01 00:00:00
abstract::An altered expression of microRNAs (miRNAs) contributes both to the development of cancer and to the progression of the disease. Malignant tumours and tumour cell lines have widespread deregulated expressions of miRNAs compared to normal tissues. In this study, we investigated the expression profiles of 340 mammalian ...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2012.1420
更新日期:2012-07-01 00:00:00
abstract::We investigated the expression and promoter methylation of dbpA in human hepatocellular carcinoma (HCC) and examined their correlation with clinicopathological features. In 96 paired samples of HCC and adjacent non-tumorous liver, and 10 normal liver specimens, dbpA mRNA was quantif...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2011.1282
更新日期:2012-03-01 00:00:00
abstract::Double-stranded RNA-dependent protein kinase (PKR) is one of the players in the cellular antiviral responses and is involved in transcriptional stimulation through activation of NF-κB. Treatment of the human osteosarcoma cell line MG63 with the protein phosphatase inhibitor okadaic acid stimulated the expression and p...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2013.1911
更新日期:2013-06-01 00:00:00
abstract::The interactions between cells and the extracellular matrix (EM) have important effects on tumor invasion and metastasis. Osteosarcoma (OS) is a highly metastatic tumor, secondary lesions occurring early in the natural history of the disease. Despite the clinical relevance of disseminated disease in this neoplasia, th...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.3.5.963
更新日期:1993-11-01 00:00:00
abstract::From January 1992 to December 1995, 129 patients with previously untreated non-Hodgkin's lymphoma were randomised in a phase III multicenter trial to receive CEOP-B/VIMB or ProMACE-CytaBOM. Eligibility criteria included intermediate or high grade lymphoma (follicular large cell, diffuse small cleaved-cell, diffuse mix...
journal_title:International journal of oncology
pub_type: 临床试验,杂志文章,多中心研究,随机对照试验
doi:10.3892/ijo.16.1.149
更新日期:2000-01-01 00:00:00
abstract::In normal human somatic cells, gradual shortening of telomeres may activate the complex cascade of molecular events known as cellular senescence. Experimental evidence from our laboratory suggests that cellular mortality is regulated by two separate mechanisms that we have termed mortality stage 1 (M1) and mortality s...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.3.4.559
更新日期:1993-10-01 00:00:00
abstract::The cytosine analogue 5'-azacytidine (5'-aza) induces DNA hypomethylation by inhibiting DNA methyltransferase. In clinical trials, 5'-aza is widely used in epigenetic anticancer treatments. Accumulated evidence shows that cyclooxygenase-2 (COX-2) is overexpressed in various cancers, indicating that it may play a criti...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2015.3110
更新日期:2015-10-01 00:00:00
abstract::Glutamate is the major excitatory neurotransmitter in the central nervous system (CNS) and binds to a variety of receptors, which recently have also been detected in peripheral, non-excitable cells. New research suggests that this abundant amino acid might also be involved in the growth of tumor cells acting via novel...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2005-09-01 00:00:00
abstract::We have utilized a series of glioma cell lines to study the effects of ionizing radiation on the regulation of proteins that contribute to cell cycle progression. While no alterations of cyclin E or cdk4 were detected, a high percentage of glioma cell lines exhibited constitutive overexpression of cdk2 protein and abe...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.10.1.93
更新日期:1997-01-01 00:00:00
abstract::Pancreatic cancer is characterized by a dense desmoplastic reaction in which extracellular matrix proteins accumulate and surround tumor cells. Integrins and their related signaling molecules are associated with progression of pancreatic cancer. In the present study, the association between the metastasis of pancreati...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2017.4018
更新日期:2017-07-01 00:00:00
abstract::Photodynamic therapy (PDT) following lung-sparing extended pleurectomy for malignant pleural mesothelioma (MPM) has been investigated as a potential means to kill residual microscopic cells. High expression levels of folate receptor 1 (FOLR1) have been reported in MPM; therefore, targeting FOLR1 has been considered a ...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2018.4555
更新日期:2018-11-01 00:00:00
abstract::Cell lines established from the TH-MYCN transgenic murine model of neuroblastoma are a valuable preclinical, immunocompetent, syngeneic model of neuroblastoma, for which knowledge of their p53 pathway status is important. In this study, the Trp53 status and functional response to Nutlin-3 and ionising radiation (IR) w...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2018.4261
更新日期:2018-03-01 00:00:00
abstract::Methylenetetrahydrofolate reductase (MTHFR) catalyzes the synthesis of 5-methyltetrahydrofolate, which is involved in the methylation of homocysteine to methionine. Genetic polymorphisms that decrease MTHFR activity result in an altered cancer risk depending on folic acid intake. In this study we examined the C677T an...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2004-11-01 00:00:00
abstract::Inflammatory abnormalities have been implicated in the pathogenesis of various human diseases, including cancer. Interleukin-1 receptor antagonist (IL1RN) is a potent anti-inflammatory molecule that modulates the biological activity of the proinflammatory cytokine, interleukin-1. The aim of this study was to examine t...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2015.2917
更新日期:2015-05-01 00:00:00
abstract::Gicerin, an immunoglobulin superfamily protein, is expressed abundantly in the embryonic tissues and plays an important role in development through its cell adhesive activity. Interestingly, re-expression of gicerin is found in a variety of tumors. In the present study, the possible role of gicerin in the progression ...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2003-12-01 00:00:00
abstract::Astrocytomas are intracranial malignancies for which invasive growth and high motility of tumour cells preclude total resection; the tumours usually recur in a more aggressive and, eventually, lethal form. Clinical outcome is highly variable and the accuracy of morphology-based prognostic statements is limited. In ord...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2006-11-01 00:00:00
abstract::MicroRNAs (miRNAs) represent a class of small non-coding RNAs regulating gene expression by inducing RNA degradation or interfering with translation. Aberrant miRNA expression has been described for several human malignancies. Herein, we show that miR-145 is down-regulated in human cancer cell line MCF-7 when compared...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2009-05-01 00:00:00
abstract::CA IX is a tumor-associated transmembrane isoform of the carbonic anhydrase with a high enzyme activity and a functional involvement in the pH regulation and cell adhesion. Expression of CA9 gene in tumor cells is principally regulated by the high cell density and the hypoxia-related VHL-HIF pathway. In renal cell car...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2005-04-01 00:00:00
abstract::Acquired drug resistance to chemotherapy is a major problem in the treatment of cancer. After primary surgical intervention, followed by chemotherapy treatments, the majority of patients develop disease recurrence. This is due to tumor cell heterogeneity and genetic instability. In contrast to tumor cells, proliferati...
journal_title:International journal of oncology
pub_type: 杂志文章,评审
doi:
更新日期:2005-08-01 00:00:00