Treadmill exercise's reduction of Agouti-related protein expression in rat liver.

Abstract:

:Agouti-related protein (AGRP) is an orexigenic peptide secreted from the arcuate nucleus (ARC) of the hypothalamus. AGRP increases food intake and plays a role in energy balance, adiposity, weight gain, and growth-hormone release. The objective of the current study was to examine the effects of running exercise on resting hepatic, fundus, and pancreas AGRP mRNA expression, as well as liver glycogen and ATP contents, using a rat model. Twenty adult male Wistar rats (12-14 wk old, 200-220 g) were randomly assigned to control (n = 10) and training (n = 10) groups. The training group was exercised for 8 wk on a motor-driven treadmill (26 m/min, 0% grade, 60 min, 5 d/wk). Twenty-four hours before sacrifice the rats were further divided into fed control (FEC), fed trained (FET), fasted control (FAC), and fasted trained (FAT) groups. The liver, fundus, and pancreas were excised and frozen in liquid nitrogen for later analysis. Results demonstrated that 8 wk of treadmill exercise reduced hepatic but not fundus and pancreatic AGRP expression and enhanced glycogen and ATP concentrations (p < .001) in trained-rat liver, whereas fasting lowered liver glycogen and ATP levels and increased hepatic AGRP mRNA expression in nonexercising controls. Data indicate that both treadmill-exercise-induced decrease and fast-induced increase in rat liver AGRP expression might depend on liver glycogen content as an important source for energy provision.

authors

Ghanbari-Niaki A,Fathi R,Kakhak SA,Farshidi Z,Barmaki S,Rahbarizadeh F,Kraemer RR

doi

10.1123/ijsnem.19.5.473

subject

Has Abstract

pub_date

2009-10-01 00:00:00

pages

473-84

issue

5

eissn

1526-484X

issn

1543-2742

journal_volume

19

pub_type

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