Abstract:
:GASA-like genes form multigene families in diverse plant species and encode the proteins with a unique cysteine-rich domain (GASA domain). In our previously work, we cloned a GASA-like gene PRGL (Proline-rich GASA-like) from gerbera. Here we report the expression profiles of PRGL and the subcellular localization of PRGL protein. Multiple sequence alignment of the GASA domains indicates that PRGL shows the highest homology to AtPRGL (73.3% of amino acid identity) from Arabidopsis. Phylogenic analysis based on the full amino acid sequences indicates that PRGL and AtPRGL belong to a novel subfamily of GASA proteins. Northern blot assay showed that PRGL is highly expressed in young flower, young leaf and young root, whereas hardly detected when these organs became mature. Furthermore, in young inflorescence, PRGL transcript accumulation only occurred in the fast elongation organs such as scape, ray floret petal and disc floret petal. Western blot and immunolocalization assay revealed that PRGL protein is located in cell wall and high level accumulation of PRGL was found to correlate with the fast organ elongation in scape. Our results suggest that PRGL participates in the regulation of cell elongation during the development of Gerbera hybrida plant.
journal_name
Mol Biol Repjournal_title
Molecular biology reportsauthors
Peng J,Lai L,Wang Xdoi
10.1007/s11033-009-9917-4subject
Has Abstractpub_date
2010-10-01 00:00:00pages
3311-7issue
7eissn
0301-4851issn
1573-4978journal_volume
37pub_type
杂志文章abstract::Endometriosis is a polygenic and multifactorial gynecology situation which might be associated with angiogenesis. In the current study we assess the role of vascular endothelial growth factor (VEGF) - 2578 A/C, and + 936 C/T polymorphisms in susceptibility to endometriosis and checking the expression of VEGF mRNA in e...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-019-04807-6
更新日期:2019-06-01 00:00:00
abstract::The aim of this study was to determine whether tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) promoter polymorphisms confer susceptibility to periodontitis in ethnically different populations. A literature search was performed using PubMed and Embase and a meta-analysis of the identified studies was conducte...
journal_title:Molecular biology reports
pub_type: 杂志文章,meta分析
doi:10.1007/s11033-013-2621-4
更新日期:2013-08-01 00:00:00
abstract::The objective of this study was to estimate the impact of the polymorphism of μ-calpain (CAPN1S) gene on protein changes of the cattle muscle tissue and its tenderness during 10-day cold storage. The analysis was performed on the longest dorsal and lumbar muscles collected from 76 bulls 6 to 12 months of age. Polymorp...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-010-0229-5
更新日期:2011-02-01 00:00:00
abstract::The degeneration of retinal pigment epithelium (RPE) cells in the sub retinal pigment epithelial space and choroid is an initial pathological characteristic for the age-related macular degeneration which is the leading cause of severe vision loss in old people. Moreover, oxidative stress is implicated as a major induc...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-016-4041-8
更新日期:2016-10-01 00:00:00
abstract::Knowledge in the area of genetic diversity could aid in providing useful information in the selection of material for breeding such as hybridization programs and quantitative trait loci mapping. To this end, 50 Nicotiana tabacum genotypes were genotyped with 21 primer combination of amplified fragment length polymorph...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-014-3194-6
更新日期:2014-05-01 00:00:00
abstract::Rice bran oil is good quality edible oil, rich in antioxidants and comprised typically of oleic-linoleic type fatty acids. However, presence of a highly lipolytic enzyme Phospholipase D alpha1 (OsPLDα1) increases free fatty acid content in the oil which further leads to stale flavor and rancidity of the oil, making it...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-019-05144-4
更新日期:2020-01-01 00:00:00
abstract::The interaction between ovalbumin (OVA) and three purine alkaloids (caffeine, theophylline and diprophylline) was investigated by the aid of intrinsic and synchronous fluorescence, ultraviolet-vis absorbance, resonance light-scattering spectra and three-dimensional fluorescence spectra techniques. Results showed that ...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-012-2418-x
更新日期:2013-04-01 00:00:00
abstract::Four housekeeping genes named Lbbeta-Actin1, Lbbeta-Actin2, Lbalpha-Tubulin, and LbGapdh were cloned from Liposcelis bostrychophila using the combined techniques of reverse transcriptase-PCR (RT-PCR) with rapid amplification of cDNA ends (RACE). The GenBank accession numbers were FJ196622, FJ447483, FJ595242, and FJ59...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-009-9803-0
更新日期:2010-02-01 00:00:00
abstract::Potato (Solanum tubersosum L.) is relatively vulnerable to abiotic stress conditions such as drought, but the tolerance mechanisms to such stress in potato are largely unknown. To gain insight into the transcriptome dynamics that are associated with drought stress, genome-wide gene expression profile was conducted by ...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-013-2886-7
更新日期:2014-01-01 00:00:00
abstract::We report here a cDNA and its deduced amino acid sequence encoding a cathepsin D-like, aspartic protease from Chlamys farreri (denoted as CfCD) by expressed sequence tag and rapid amplification of cDNA ends techniques. The cDNA of CfCD consisted of 1,810 nucleotides with a canonical polyadenylation signal sequence AAT...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-009-9534-2
更新日期:2010-03-01 00:00:00
abstract::Autoantibodies directed to a variety of cellular antigens and organelles are a feature of autoimmune diseases. They have proven useful in a clinical setting to establish diagnosis, estimate prognosis, follow disease progression, alter therapy, and initiate new investigations. Cellular and molecular biologists have use...
journal_title:Molecular biology reports
pub_type: 杂志文章,评审
doi:10.1023/a:1016523013819
更新日期:1998-07-01 00:00:00
abstract::Gonadal dysgenesis (GD) is a rare cause of differences of sex development (DSD) with highly variable clinical and genetic conditions. Although identification of the causative genetic alterations can offer a clearer prognosis and personalized management to patients, more than 50% of the DSD cases still do not have an a...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-019-04980-8
更新日期:2019-10-01 00:00:00
abstract::J The [Ca2+] regulation of contractile ATPase flux, Jp, in skeletal muscle was analysed by computation of the Response R(Jp) Ca2+ for a 10 Hz range of electrical stimulation frequencies. Results of our analysis of the kinetic controls in ATP free energy metabolism in a network model of contracting muscle (J.A.L. Jenes...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1023/a:1020317610863
更新日期:2002-01-01 00:00:00
abstract::This study is to explore the relationship between vascular endothelial growth factor (VEGF) and pathological changes in cryptorchidism by using murine model of intraperitoneal cryptorchidism with surgical operation. To investigate the relationship between the changes of VEGF expression and heat stress inducing germ ce...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-020-05454-y
更新日期:2020-05-01 00:00:00
abstract::The mRNA differential display technique was performed to investigate the differences of gene expression in the longissimus muscle tissues from Meishan and Large White pigs. One novel mRNA that was differentially expressed was identified through semi-quantitative RT-PCR and the cDNA complete sequence was then obtained ...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-008-9326-0
更新日期:2009-07-01 00:00:00
abstract::As-ClC (chloride channels protein from Artemia sinica), a member from the chloride channels protein family, is a α-helical membrane protein predicted to traverse the cell membrane 11 times. It is important for several physiological functions such as cell volume regulation, cell proliferation, growth and differentiatio...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-012-2441-y
更新日期:2013-05-01 00:00:00
abstract::Bacterial artificial chromosome (BAC) library is an important genomic resource useful in targeted marker development, positional cloning, physical mapping and a substrate for genome sequencing for better understanding the genome organization of a species. The present manuscript elucidates the improvement in protocols ...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-020-05283-z
更新日期:2020-03-01 00:00:00
abstract::Vitiligo is a common chronic depigmented skin disease characterized by melanocyte loss or dysfunction in the lesion. The pathogenesis of vitiligo has not been fully clarified. Most studies have suggested that the occurrence and progression of vitiligo are due to multiple factors and gene interactions in which noncodin...
journal_title:Molecular biology reports
pub_type: 杂志文章,评审
doi:10.1007/s11033-020-05336-3
更新日期:2020-04-01 00:00:00
abstract::Protein phosphorylation is one of the most fundamental types of post-translational modifications and it plays a vital role in various cellular processes of eukaryotes. Among three types of phosphorylation i.e. serine, threonine and tyrosine phosphorylation, tyrosine phosphorylation is one of the most frequent and it i...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-018-4417-z
更新日期:2018-12-01 00:00:00
abstract::To determine the plasma lipid and lipoprotein profiles and their possible association with the type of β-thalassemia mutation we studied 103 major β-thalassemia patients including 71 children and 32 young adults compared to 102 healthy subjects consisted of 90 children and 12 young healthy adults. The plasma lipid and...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-010-0397-3
更新日期:2011-04-01 00:00:00
abstract::Cell-based approaches offer a potential therapeutic strategy for appropriate bone manufacturing. Capable of differentiating into multiple cell types especially osteoblasts spontaneously, unrestricted somatic stem cell (USSC) seems to be a suitable candidate. Recent studies have shown the involvement of microRNAs in se...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-012-1591-2
更新日期:2012-07-01 00:00:00
abstract::The current study represents the first molecular characterization of freshwater fish species in Egypt from two major fish resources; the River Nile and Lake Nasser. A total of 160 DNA barcodes using a 655-bp-long fragment of the mitochondrial cytochrome oxidase subunit I (COI) gene were generated from 37 species belon...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-020-05657-3
更新日期:2020-08-01 00:00:00
abstract:AIM:To study the function of the prodomain of ADAM17 (TACE) and to develop an approach for interfering with inflammation processes. METHOD:The expression plasmids of the TACE ectodomain (T1300), prodomain (T591), signal peptide and prodomain (T648), full length (T2472), and the turncated TACE without prodomain (T57-T1...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-008-9224-5
更新日期:2009-04-01 00:00:00
abstract::Several lines of evidence suggest that the ubiquitin-proteasome pathway is involved in sepsis-induced muscle catabolism. The gene expression of ubiquitin and several of the proteasome subunits was increased in muscle from both septic rats and patients. In other studies, the activity of isolated 20S proteasomes was sti...
journal_title:Molecular biology reports
pub_type: 杂志文章,评审
doi:10.1023/a:1006916206260
更新日期:1999-04-01 00:00:00
abstract::The Cichlid fish Sarotherodon melanotheron is typically found in West and Central African estuaries and lagoons. It represents a good candidate for promoting tilapia farming in brackish waters. Understanding the genetic diversity in its populations from the hydrographical basins of Southern Benin is primordial before ...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-018-4354-x
更新日期:2018-12-01 00:00:00
abstract::Rheumatoid arthritis (RA) and ankylosing spondylitis (AS) are autoimmune, inflammatory diseases with substantial genetic contributions. Matrix metalloproteinase (MMP)-2, tumor necrosis factor (TNF)-α and NLR family pyrin domain-containing 1 (NLRP1) play important roles in the immune response. We studied the MMP-2 rs24...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-013-2743-8
更新日期:2013-11-01 00:00:00
abstract::The researches on yak genetics and breeding were extremely restricted due to lacking of reliable DNA molecular markers. The microsatellites with repeat motif (AC)n/(GT)n in yak genome were enriched by Dynal magnetic beads and the gene libraries containing (AC)n/(GT)n were constructed. Among the 92 identified and seque...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-014-3249-8
更新日期:2014-06-01 00:00:00
abstract::A double-stranded RNA-specific nuclease (ds RNase) has been isolated and partially purified from human placenta by DEAE-cellulose and DNA-cellulose column chromatography. Denatured DNA-cellulose retained most of the single-stranded RNA-specific nuclease (ss RNase) activity, whereas the ds RNase came out in the void vo...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/BF00775365
更新日期:1983-08-01 00:00:00
abstract::Keratin-associated proteins (KAPs) are structural components of wool and variation in them may affect wool characteristics. In this study, we used PCR-SSCP to analyse the ovine KAP6 family which encodes glycine and tyrosine-rich KAPs. Five unique PCR-SSCP patterns were detected in the 250 sheep investigated. Between t...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-010-0074-6
更新日期:2011-01-01 00:00:00
abstract::It has been suggested that the allele frequency of thrombophilic mutations is affected by glucose-6-phosphate dehydrogenase (G6PD) deficiency. The prevalence of thrombophilic mutations were studied in sixty G6PD deficient individuals including 57 males and three females with the mean age of 15 +/- 3.08 and 110 age and...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-009-9458-x
更新日期:2009-11-01 00:00:00