Soft X-ray tomography of phenotypic switching and the cellular response to antifungal peptoids in Candida albicans.

abstract：:Distinguishing tumor from normal glandular breast tissue is an important step in breast-conserving surgery. Because this distinction can be challenging in the operative setting, up to 40% of patients require an additional operation when traditional approaches are used. Here, we present a proof-of-concept study to dete...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Calligaris D,Caragacianu D,Liu X,Norton I,Thompson CJ,Richardson AL,Golshan M,Easterling ML,Santagata S,Dillon DA,Jolesz FA,Agar NY

更新日期：2014-10-21 00:00:00

abstract：:In Drosophila, Pumilio (Pum) is important for neuronal homeostasis as well as learning and memory. We have recently characterized a mammalian homolog of Pum, Pum2, which is found in discrete RNA-containing particles in the somatodendritic compartment of polarized neurons. In this study, we investigated the role of Pum...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Vessey JP,Schoderboeck L,Gingl E,Luzi E,Riefler J,Di Leva F,Karra D,Thomas S,Kiebler MA,Macchi P

更新日期：2010-02-16 00:00:00

abstract：:Seven morphologically nontransformed (flat) revertants with reduced tumorigenicity in vivo have been isolated from populations of Kirsten sarcoma virus-transformed NIH 3T3 cells transfected with a cDNA expression library of normal human fibroblasts. Each revertant harbors 1-10 recombinant plasmids per cell and retains...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Noda M,Kitayama H,Matsuzaki T,Sugimoto Y,Okayama H,Bassin RH,Ikawa Y

更新日期：1989-01-01 00:00:00

abstract：:Human plasma has been shown to contain an apolipoprotein that mediates the transport of cholesteryl ester from high density lipoprotein (HDL) to very low density lipoprotein (VLDL) or low density lipoprotein (LDL). This activity, confined to the density greater than 1.063 g/ml interval, has been isolated from HDL and ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chajek T,Fielding CJ

更新日期：1978-07-01 00:00:00

abstract：:Combinations of molecular tags visible in light and electron microscopes become particularly advantageous in the analysis of dynamic cellular components like the Golgi apparatus. This organelle disassembles at the onset of mitosis and, after a sequence of poorly understood events, reassembles after cytokinesis. The pr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gaietta GM,Giepmans BN,Deerinck TJ,Smith WB,Ngan L,Llopis J,Adams SR,Tsien RY,Ellisman MH

更新日期：2006-11-21 00:00:00

abstract：:Individuals homozygous for the C-C chemokine receptor type 5 gene with 32-bp deletions (CCR5Δ32) are resistant to HIV-1 infection. In this study, we generated induced pluripotent stem cells (iPSCs) homozygous for the naturally occurring CCR5Δ32 mutation through genome editing of wild-type iPSCs using a combination of ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ye L,Wang J,Beyer AI,Teque F,Cradick TJ,Qi Z,Chang JC,Bao G,Muench MO,Yu J,Levy JA,Kan YW

更新日期：2014-07-01 00:00:00

abstract：:The 1987 Montreal Protocol on Substances that Deplete the Ozone Layer is a landmark agreement that has successfully reduced the global production, consumption, and emissions of ozone-depleting substances (ODSs). ODSs are also greenhouse gases that contribute to the radiative forcing of climate change. Using historical...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Velders GJ,Andersen SO,Daniel JS,Fahey DW,McFarland M

更新日期：2007-03-20 00:00:00

abstract：:IL-17A is a therapeutic target in many autoimmune diseases. Most nonhematopoietic cells express IL-17A receptors and respond to extracellular IL-17A by inducing proinflammatory cytokines. The IL-17A signal transduction triggers two broad, TRAF6- and TRAF5-dependent, intracellular signaling pathways to produce represen...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： McGowan J,Peter C,Kim J,Popli S,Veerman B,Saul-McBeth J,Conti H,Pruett-Miller SM,Chattopadhyay S,Chakravarti R

更新日期：2020-10-06 00:00:00

abstract：:The medial preoptic area has been shown to be intricately involved in many behaviors, including locomotion, sexual behavior, maternal care, and aggression. The gene encoding estrogen receptor-α (ERα) protein is expressed in preoptic area neurons, and a very dense immunoreactive field of ERα is found in the preoptic re...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ribeiro AC,Musatov S,Shteyler A,Simanduyev S,Arrieta-Cruz I,Ogawa S,Pfaff DW

更新日期：2012-10-02 00:00:00

abstract：:III-V compound semiconductors are widely used for electronic and optoelectronic applications. However, interfacing III-Vs with other materials has been fundamentally limited by the high growth temperatures and lattice-match requirements of traditional deposition processes. Recently, we developed the templated liquid-p...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hettick M,Li H,Lien DH,Yeh M,Yang TY,Amani M,Gupta N,Chrzan DC,Chueh YL,Javey A

更新日期：2020-01-14 00:00:00

abstract：:A total of 289,868 locus tests, based on 28 different protein phenotypes and using one-dimensional electrophoresis to detect variant proteins, has yielded one probable mutation in the offspring of "proximally exposed" parents, who received an estimated average gonadal exposure of 31 to 39 rem in the atomic bombings of...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Neel JV,Satoh C,Hamilton HB,Otake M,Goriki K,Kageoka T,Fujita M,Neriishi S,Asakawa J

更新日期：1980-07-01 00:00:00

abstract：:Modeling studies of terrestrial extrasolar planetary climates are now including the effects of ocean circulation due to a recognition of the importance of oceans for climate; indeed, the peak equator-pole ocean heat transport on Earth peaks at almost half that of the atmosphere. However, such studies have made the ass...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cullum J,Stevens DP,Joshi MM

更新日期：2016-04-19 00:00:00

abstract：:Examples from various plant and animal groups indicate that there has been a general increase in potential versatility of form, determined by the number and range of independently varying morphogenetic parameters, among taxa appearing at successively younger stages in the fossil record. Taxa or body plans with higher ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Vermeij GJ

更新日期：1973-07-01 00:00:00

abstract：:Phosphorylation of ADP to ATP was induced in nonrespiring submitochondrial particles (SMP) from rat liver by the application of electric pulses with field strengths of 10-35 kV/cm and a decay time of 60 mus. In all cases respiration was inhibited completely by using cyanide or rotenone. Newly formed ATP was measured b...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Teissie J,Knox BE,Tsong TY,Wehrle J

更新日期：1981-12-01 00:00:00

abstract：:The DNA sequences of wheat Acc-1 and Acc-2 loci, encoding the plastid and cytosolic forms of the enzyme acetyl-CoA carboxylase, were analyzed with a view to understanding the evolution of these genes and the origin of the three genomes in modern hexaploid wheat. Acc-1 and Acc-2 loci from each of the wheats Triticum ur...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chalupska D,Lee HY,Faris JD,Evrard A,Chalhoub B,Haselkorn R,Gornicki P

更新日期：2008-07-15 00:00:00

abstract：:Sarcoplasmic reticulum vesicles were exposed to various thiol-directed spin labels, and the position of the label on the inner or outer vesicle surface was investigated as a function of the ATPase (adenosinetriphosphatase; ATP phosphohydrolase, EC 3.6.1.3) chemical state. Previous measurements of label accessibility t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Champeil P,Rigaud JL,Gary-Bobo CM

更新日期：1980-05-01 00:00:00

abstract：:A 92-amino acid polypeptide, alpha-inhibin-92 (alpha-IB-92), has been isolated and characterized from human seminal plasma and found to be active in suppressing follicle-stimulating hormone (FSH) release in vitro. In the present in vivo study, intravenous injection of synthetic alpha-IB-92 (4 and 20 micrograms) signif...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yu WH,McCann SM,Li CH

更新日期：1988-01-01 00:00:00

abstract：:A 1H NMR study of the 79-residue globular domain of chicken erythrocyte histone H5 (GH5) is presented. Using a combination of two-dimensional NMR techniques to demonstrate through-bond and through-space (less than 5 A) connectivities, the resonances of GH5 are assigned in a sequential manner. From a qualitative interp...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zarbock J,Clore GM,Gronenborn AM

更新日期：1986-10-01 00:00:00

abstract：:In vitro translation of Rous sarcoma virus (RSV) virion RNA in the messenger-dependent reticulocyte lysate system yielded polypeptides that were not synthesized by translation of RNA from a transformation-defective deletion mutant of RSV. These RSV-specific products migrated on sodium dodecyl sulfate/polyacrylamide ge...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Beemon K,Hunter T

更新日期：1977-08-01 00:00:00

abstract：:Infection by Plasmodium, the causative agent of malaria, is associated with hemolysis and therefore with release of hemoglobin from RBC. Under inflammatory conditions, cell-free hemoglobin can be oxidized, releasing its heme prosthetic groups and producing deleterious free heme. Here we demonstrate that survival of a ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Seixas E,Gozzelino R,Chora A,Ferreira A,Silva G,Larsen R,Rebelo S,Penido C,Smith NR,Coutinho A,Soares MP

更新日期：2009-09-15 00:00:00

abstract：:The activity of leukotriene A4 (LTA4) synthase in crude human leukocyte homogenates was found to have a similar requirement for Ca2+ and ATP as had been noted previously for 5-lipoxygenase activity. Purification of the 5-lipoxygenase using ammonium sulfate fractionation, AcA 44 gel-filtration chromatography, and HPLC ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rouzer CA,Matsumoto T,Samuelsson B

更新日期：1986-02-01 00:00:00

abstract：:beta 1-Adrenergic receptors from turkey erythrocyte membranes have been identified by specific binding of the radiolabeled antagonist (-)-[3H]dihydroalprenolol. These receptors are inactivated by the alkylating agent N-ethylmaleimide when occupied by beta-adrenergic agonists but not when occupied by antagonists or whe...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Vauquelin G,Bottari S,Andre C,Jacobsson B,Strosberg AD

更新日期：1980-07-01 00:00:00

abstract：:Murine embryonal carcinoma (EC) cells are refractory to infection by retroviruses because retroviral long terminal repeat (LTR) enhancers have little activity in EC cells. A previous report described the isolation of clonal EC cell lines that express the integrated neomycin-resistance gene (neo) linked to the Moloney ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Taketo M,Tanaka M

更新日期：1987-06-01 00:00:00

abstract：:Communication about prescription drugs ought to be a paragon of public science communication. Unfortunately, it is not. Consumers see $4 billion of direct-to-consumer advertising annually, which typically fails to present data about how well drugs work. The professional label--the Food and Drug Administration's (FDA) ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schwartz LM,Woloshin S

更新日期：2013-08-20 00:00:00

abstract：:Drosophila Numb is a membrane associated protein of 557 amino acids (aa) that localizes asymmetrically into a cortical crescent in mitotic neural precursor cells and segregates into one of the daughter cells, where it is required for correct cell fate specification. We demonstrate here that asymmetric localization but...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Knoblich JA,Jan LY,Jan YN

更新日期：1997-11-25 00:00:00

abstract：:Modified bovine leukemia virus (BLV) glycoproteins were expressed by using vaccinia virus recombinants, and their fusogenic capacities were examined by a syncytia-formation assay. This analysis indicates that (i) both BLV envelope glycoproteins gp51 and gp30 are necessary for cell fusion; (ii) insertion of the N-termi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Vonèche V,Portetelle D,Kettmann R,Willems L,Limbach K,Paoletti E,Ruysschaert JM,Burny A,Brasseur R

更新日期：1992-05-01 00:00:00

abstract：:Biological membranes contain an extraordinary diversity of lipids. Phospholipids function as major structural elements of cellular membranes, and analysis of changes in the highly heterogeneous mixtures of lipids found in eukaryotic cells is central to understanding the complex functions in which lipids participate. P...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ivanova PT,Cerda BA,Horn DM,Cohen JS,McLafferty FW,Brown HA

更新日期：2001-06-19 00:00:00

abstract：:Many experiments have been done to determine how far and how freely holes can move along the stack of base pairs in DNA. The results of these experiments are usually described in terms of a parameter beta under the assumption that it describes an exponential decay with distance. The reported values range from beta < 0...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Conwell EM,Rakhmanova SV

更新日期：2000-04-25 00:00:00

abstract：:We have demonstrated the existence of human cytokine synthesis inhibitory factor (CSIF) [interleukin 10 (IL-10)]. cDNA clones encoding human IL-10 (hIL-10) were isolated from a tetanus toxin-specific human T-cell clone. Like mouse IL-10, hIL-10 exhibits strong DNA and amino acid sequence homology to an open reading fr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Vieira P,de Waal-Malefyt R,Dang MN,Johnson KE,Kastelein R,Fiorentino DF,deVries JE,Roncarolo MG,Mosmann TR,Moore KW

更新日期：1991-02-15 00:00:00

abstract：:The prion protein (PrP) is a scrapie-associated fibril protein that accumulates in the brains of hamsters and mice infected with the scrapie agent, and also in the brains of persons affected with kuru or Creutzfeldt-Jakob disease. It has been previously proposed that PrP could be either the primary transmissible agent...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Locht C,Chesebro B,Race R,Keith JM

更新日期：1986-09-01 00:00:00