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Efficient expansion of mesenchymal stromal cells from umbilical cord under low serum conditions.

Abstract：

BACKGROUND:Mesenchymal stromal cells (MSC) are of clinical interest for their potential use in regenerative medicine and immunotherapy. Originally derived from bone marrow (BM), MSC have now been isolated from most tissues, including umbilical cord (UC) and UC blood (UCB). If MSC from UC are biologically equivalent to those from BM, they would be attractive as a readily available and non-invasive source for cellular therapies. METHODS:Sections of UC were separated into vascular and Wharton's jelly (WJ) fractions, which were then digested individually to release MSC that were isolated by plastic adherence in a 10% fetal calf serum (FCS) medium, or a low serum medium designed for multipotent adult progenitor cells (MAPC). The resulting perivascular (PV) and WJ MSC lines were assayed for expression of characteristic markers and differentiation and immunosuppressive properties. RESULTS:MSC lines were readily derived from most UC tested. Cells grown in MAPC medium (MM) tended to be smaller and more elongated and expressed more nestin, but did not differ substantially in their growth rate, expression of other markers and differentiation capacity. All UC lines tested were adipogenic but poorly osteogenic, and were equivalent in their ability to suppress T-cell proliferation induced by phytohemagglutinin (PHA), activation beads and allostimulation. CONCLUSIONS:UC is a convenient, efficient source of MSC that can be expanded under low serum conditions for application on future studies of tissue regeneration and immunosuppression.

journal_name

Cytotherapy

journal_title

Cytotherapy

authors

Girdlestone J,Limbani VA,Cutler AJ,Navarrete CV

doi

10.3109/14653240903079401

subject

Has Abstract

pub_date

2009-01-01 00:00:00

pages

738-48

issue

6

eissn

1465-3249

issn

1477-2566

pii

10.3109/14653240903079401

journal_volume

11

pub_type

杂志文章

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