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Preparation of purified lymphoma cells suitable for therapy.

Abstract：

BACKGROUND:Very few tumoral Ags have yet been isolated in NHL B cells. It is nevertheless possible to use whole tumor cells as a source of tumor Ags. We describe the purification of large numbers of human NHL B cells directly from lymph node or spleen biopsies, and different preparations allowing their use in a clinical setting. METHODS:The purification procedure consists of the negative selection of tumor B cells: cells to be eliminated are opsonized by CD2 Abs, and then coupled to magnetic beads for separation by the Isolex 300 magnetic separator. RESULTS:The mean yield of the purification was 74% for CD19+ cells, with a mean purity of 87%, dependent on the initial fraction of tumor cells in the biopsy. Using this procedure, a large number of purified tumor cells can be recovered from a biopsy in sterile conditions. We also describe treatments of B cells that can enhance their uptake by APCs, a critical step in anti-tumor immunotherapy strategies. Cells were opsonized by rituximab, or induced in apoptosis by irradiation, or necrosis by heating. Cell lysates were directly prepared from purified tumor cells. DISCUSSION:These procedures were reproducible on every lymphoma cell, and treated cells were phagocytosed by APCs. The methodology described here allows the evaluation of the immunological potential of apoptotic, necrotic, opsonized lymphoma cells, or their lysates, in a clinical setting.

journal_name

Cytotherapy

journal_title

Cytotherapy

authors

Lui G,Manches O,Chaperot L,Ducrot T,Molens JP,Sotto JJ,Bensa JC,Plumas J

doi

10.1080/14653240410006059

keywords:

subject

Has Abstract

pub_date

2004-01-01 00:00:00

pages

235-43

issue

3

eissn

1465-3249

issn

1477-2566

pii

EW65CNKQKQGCKJEH

journal_volume

6

pub_type

杂志文章

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