Abstract:
BACKGROUND:Traditionally, following high-dose therapy (HDT), unmanipulated autologous PBPC are infused. Alternatively, purified CD34+ cells can now be obtained by immunomagnetic separation using the CliniMACS device. Limited data currently exist examining hemopoietic recovery with such cells. METHODS:Ten patients with advanced breast cancer had PBPC mobilized with docetaxel (100 mg/m2) and G-CSF (10 microg/kg per day), harvested and processed using the CliniMACS CD34-selection device and equally divided into three aliquots for cryopreservation. Unmanipulated 'back-up' cells were also collected on a separate day of the same mobilization, divided into three and cryopreserved. Patients subsequently received three cycles of HDT with cyclophosphamide (4 g/m2), thiotepa (300 mg/m2) and paclitaxel (175 mg/m2). The intent was for patients to receive CD34-selected cells to support each of the three cycles of HDT (i.e., 1/3 for each cycle). If, however, hemopoietic recovery was delayed after Cycle 1, 1/3 of the unmanipulated cells were infused following Cycle 2 and the remaining CD34-selected cells (2/3) were used to support Cycle 3. RESULTS:PBPC from 10 patients underwent CD34-selection with a resulting median purity of 93% (range: 76-98%) and yield of 62% (range: 16-93%). Of the 10 patients, only two were able to be supported with CD34-selected cells for all three cycles of HDT. The remaining eight patients required unmanipulated 'back-up' cells to support Cycle 2. Three patients also required infusion of 'back-up' unmanipulated cells because of persistent neutropenia (n = 1) or thrombocytopenia (n = 2) following cycles initially supported by CD34-selected cells. The median number of CD34-selected cells (x 10(6)/kg) infused per cycle was 1.5 (0.7-2.6) (n = 20) and unselected cells was 1.7 (1.4-2.8) (n = 10). Comparing hemopoietic recovery between cycles of HDT supported by CD34-selected (n = 20) and unmanipulated cells (n = 10) there was a significant slowing with the CD34-selected cells; time to ANC > 1.0 = 13 days versus 10 days, platelets > 20 = 17 days versus 13 days, > 50 = 25 versus 17 days (all P values < 0.001). There was no correlation between the dose of CD34-selected cells infused and neutrophil/platelet recovery. DISCUSSION:We have demonstrated that, although unmanipulated PBPC achieve rapid hemopoietic recovery (at modest CD34 doses of < or = 2.8 x 10(6)/kg), CliniMACS-selected CD34+ cells (in the doses utilized in this study of < or = 2.6 x 10(6)/kg) result in significantly prolonged recovery.
journal_name
Cytotherapyjournal_title
Cytotherapyauthors
Prince HM,Wall D,Rischin D,Toner GC,Seymour JF,Blakey D,Haylock D,Simmons P,Wolf M,Januszewicz EH,Westerman D,Richardson G,Scarlett J,Briggs Pdoi
10.1080/146532402317381857keywords:
subject
Has Abstractpub_date
2002-01-01 00:00:00pages
147-55issue
2eissn
1465-3249issn
1477-2566pii
S1465-3249(02)71049-9journal_volume
4pub_type
临床试验,杂志文章相关文献
CYTOTHERAPY文献大全abstract:BACKGROUND AIMS:Endothelial apoptosis underlies the pathophysiology of pulmonary arterial hypertension (PAH). Some factors/cytokines released by endothelial progenitor cells (EPC) have been revealed as potent inhibitors of apoptosis. The aim of this study was to investigate the effects of EPC on pulmonary microvascular...
journal_title:Cytotherapy
pub_type: 杂志文章
doi:10.1080/14653240902960460
更新日期:2009-01-01 00:00:00
abstract:BACKGROUND AIMS:Stem cell therapy for liver diseases has recently emerged as a promising alternative to liver transplantation. Eligible cells should have an appropriate immunophenotype. The aim of the present study was to define the immunological profile of two human liver-derived mesenchymal stromal cell populations, ...
journal_title:Cytotherapy
pub_type: 杂志文章
doi:10.1016/j.jcyt.2014.10.001
更新日期:2015-02-01 00:00:00
abstract:BACKGROUND AIMS:Mesenchymal stromal cells (MSCs) have been applied to patients in cell therapy for various diseases. Recently, we introduced a novel MSC separation filter device which could yield approximately 2.5-fold more MSCs from bone marrow in a closed system compared with the conventional open density gradient ce...
journal_title:Cytotherapy
pub_type: 杂志文章
doi:10.1016/j.jcyt.2014.10.013
更新日期:2015-03-01 00:00:00
abstract:BACKGROUND AIMS:Cytotherapy is a promising option for neurodegenerative disease treatment. Because of the fatal prognosis and imperative need for effective treatment, amyotrophic lateral sclerosis (ALS) patients request this therapy before its effectiveness has been verified. The increase in clinics offering cytotherap...
journal_title:Cytotherapy
pub_type: 杂志文章
doi:10.3109/14653241003774037
更新日期:2010-09-01 00:00:00
abstract:BACKGROUND:Clinical studies have shown the efficacy of combination therapy for various malignancies. In this study, the characteristics, safety and feasibility of use of cascade-primed (CAPRI) cells for the combination treatment of non-small-cell lung cancer (NSCLC) were evaluated both in vitro and in vivo. METHODS:Si...
journal_title:Cytotherapy
pub_type: 杂志文章
doi:10.1016/j.jcyt.2019.12.002
更新日期:2020-01-01 00:00:00
abstract:BACKGROUND AIMS:Enumeration of viable CD34(+) cells provides critical information for the bone marrow (BM) transplant physician. The single-platform ISHAGE protocol is the most reliable method currently available to quantitate accurately this important subset of cells. Previous studies have shown that 5 CD34(+) cells/m...
journal_title:Cytotherapy
pub_type: 杂志文章
doi:10.1080/14653240902923161
更新日期:2009-01-01 00:00:00
abstract:BACKGROUND:There is growing interest in the use of dendritic cells (DCs) for treatment of malignancy and infectious disease. Our goal was to develop a clinical scale method to prepare autologous DCs for cancer clinical trials. METHODS:PBMC were collected from normal donors or cancer patients by automated leukapheresis...
journal_title:Cytotherapy
pub_type: 杂志文章
doi:10.1080/146532401753156377
更新日期:2001-01-01 00:00:00
abstract:BACKGROUND:Multiple studies have demonstrated that 'purging' of autografts with 4-hydroperoxycyclophosphamide (4HC) or the related compound mafosfamide (Mf), to eradicate residual leukemia, produces the best results associated with autologous blood and marrow transplantation for AML. However, 4HC purging results in pro...
journal_title:Cytotherapy
pub_type: 临床试验,杂志文章
doi:10.1080/14653240600905353
更新日期:2006-01-01 00:00:00
abstract:BACKGROUND:Cells from AML patients can differentiate into the phenotype of DC when cultured with GM-CSF and IL-4. Such cytokine-treated AML-derived DC (AML-DC) can stimulate autologous T cells. In this study we examined whether an anti-CTLA-4 MAb (MDX-010) could enhance the generation of autologous anti-AML T cells. M...
journal_title:Cytotherapy
pub_type: 杂志文章
doi:10.1080/14653240500499507
更新日期:2006-01-01 00:00:00
abstract:OBJECTIVE:The objective of this study was to investigate the effects of the intravenous transplantation of bone marrow mesenchymal stromal cells (BM-MSCs) on the repair of glomerular endothelia and angiogenesis in rats with chronic renal failure (CRF). Furthermore, the mechanism of BM-MSCs promoting angiogenesis was ex...
journal_title:Cytotherapy
pub_type: 杂志文章
doi:10.1016/j.jcyt.2016.03.300
更新日期:2016-07-01 00:00:00
abstract:BACKGROUND AIMS:Multiple cell-therapy products require density separation as a part of manufacturing. The traditional method for Ficoll separation, layering cell suspensions over Ficoll in tubes, followed by centrifugation and collection of cells from the interface, is too cumbersome and poses too high a risk of contam...
journal_title:Cytotherapy
pub_type: 杂志文章
doi:10.3109/14653240903479663
更新日期:2010-05-01 00:00:00
abstract:BACKGROUND:Current efforts to direct differentiation of human embryonic stem cells (hESC) into a particular cell lineage usually lead to a heterogeneous cell population with only a fraction of the desired cell type present. We show the generation of an essentially pure population of human cardiomyocytes from hESC using...
journal_title:Cytotherapy
pub_type: 杂志文章
doi:10.1080/14653240802105307
更新日期:2008-01-01 00:00:00
abstract:BACKGROUND AIMS:Multiple steps are required to produce chimeric antigen receptor (CAR)-T cells, involving subset enrichment or depletion, activation, gene transduction and expansion. Open processing steps that increase risk of contamination and production failure are required. This complex process requires skilled pers...
journal_title:Cytotherapy
pub_type: 杂志文章
doi:10.1016/j.jcyt.2017.09.005
更新日期:2018-03-01 00:00:00
abstract:BACKGROUND AIMS:Adipose tissue has therapeutic potential for spinal cord injury (SCI) because it contains multipotent cells known as adipose-derived stem/stromal cells (ASCs). In this study, we attempted intravenous ASC transplantation in rats with SCI to examine the effect on functional recovery. METHODS:ASCs (2.5 × ...
journal_title:Cytotherapy
pub_type: 杂志文章
doi:10.1016/j.jcyt.2017.04.002
更新日期:2017-07-01 00:00:00
abstract:BACKGROUND:Since 1978, 856 hematopoietic stem-cell transplantations have been performed for adult patients in Turkey. METHODS AND RESULTS:Data were collected on the source of hematopoietic progenitor cells, recipient diagnosis, conditioning regimen, DFS and mortality. The data were analyzed by the national registry sy...
journal_title:Cytotherapy
pub_type: 杂志文章
doi:10.1080/146532400539288
更新日期:2000-01-01 00:00:00
abstract:BACKGROUND AIMS:The authors aim to analyze the evidence in the literature regarding the efficacy and safety of mesenchymal stem cell (MSC) therapy in human subjects with traumatic spinal cord injury (SCI) and identify its potential role in the management of SCI. METHODS:The authors conducted independent and duplicate ...
journal_title:Cytotherapy
pub_type: 杂志文章,评审
doi:10.1016/j.jcyt.2020.09.007
更新日期:2020-11-09 00:00:00
abstract:BACKGROUND AIMS:Mesenchymal stromal cells (MSCs) suppress T-cell proliferation, especially after activation with inflammatory cytokines. We compared the dynamic action of unprimed and interferon (IFN)-γ plus tumor necrosis factor (TNF)-α-pretreated human bone marrow-derived MSCs on resting or activated T cells. METHOD...
journal_title:Cytotherapy
pub_type: 杂志文章
doi:10.1016/j.jcyt.2013.11.008
更新日期:2014-02-01 00:00:00
abstract:BACKGROUND AIMS:Umbilical cord blood transplantation (CBT) is an effective treatment for benign and malignant diseases. Late effects of CBT are not well described in the literature. In the present study, we present our experience of new-onset allergies in long-term survivors after CBT. METHODS:After an initial patient...
journal_title:Cytotherapy
pub_type: 杂志文章
doi:10.1016/j.jcyt.2013.05.018
更新日期:2013-10-01 00:00:00
abstract:BACKGROUND AIMS:Acute liver failure (ALF), a life-threatening disease characterized by the sudden loss of hepatic function, can occur after an accidental or intentional acetaminophen overdose. METHODS:With the use of an ALF mouse model, we examined both the preventive and therapeutic potential of intravenously adminis...
journal_title:Cytotherapy
pub_type: 杂志文章
doi:10.1016/j.jcyt.2014.05.018
更新日期:2014-09-01 00:00:00
abstract::Chimeric antigen receptor (CAR) T-cell therapy is an investigational immunocellular therapy that reprograms a patient's cytotoxic T cells to engage and eliminate malignant cells. CAR T-cell therapies targeting the CD19 antigen have demonstrated high efficacy in clinical trials for patients with B-cell malignancies and...
journal_title:Cytotherapy
pub_type: 杂志文章,评审
doi:10.1016/j.jcyt.2017.06.001
更新日期:2017-09-01 00:00:00
abstract:BACKGROUND:We have previously demonstrated that bovine dialyzable leukocyte extract (bDLE) induces death through an apoptosis mechanism in MCF-7 breast cancer cells. Depending on the cell type and stimulus, activating protein-1 (AP-1) has been shown to regulate cell proliferation and differentiation, the stress respons...
journal_title:Cytotherapy
pub_type: 杂志文章
doi:10.1080/14653240801891659
更新日期:2008-01-01 00:00:00
abstract:BACKGROUND:Amniotic membrane is a highly promising cell source for tissue engineering. Being part of the placenta, this tissue is abundantly available. It can be processed easily to yield large amounts of epithelial and mesenchymal cells that have shown broad differentiation potential. For tissue-engineering purposes, ...
journal_title:Cytotherapy
pub_type: 杂志文章
doi:10.1080/14653240802345804
更新日期:2008-01-01 00:00:00
abstract:BACKGROUND AIMS:The graft-healing potential of mesenchymal stromal cells (MSCs) derived from the remnants of ruptured anterior cruciate ligaments (ACLs) after ACL reconstruction may depend on the chronicity of the injury. The aim of this study was to assess the quantitative and phenotypic differences between MSCs isola...
journal_title:Cytotherapy
pub_type: 杂志文章
doi:10.1016/j.jcyt.2013.08.001
更新日期:2014-05-01 00:00:00
abstract:BACKGROUND AIMS:Hepatic stellate cells (HSCs) are liver-resident mesenchymal cells involved in essential processes in the liver. However, knowledge concerning these cells in human livers is limited because of the lack of a simple isolation method. METHODS:We isolated fetal and adult human liver cells by immunomagnetic...
journal_title:Cytotherapy
pub_type: 杂志文章
doi:10.1016/j.jcyt.2014.03.001
更新日期:2014-07-01 00:00:00
abstract::Allogeneic hematopoietic cell transplantation (HCT) is an important therapeutic option for a variety of malignant and non-malignant disorders (NMD). The use of umbilical cord blood transplantation (UCBT) has made HCT available to many more patients. The increased level of human leukocyte antigen disparity that can be ...
journal_title:Cytotherapy
pub_type: 杂志文章,评审
doi:10.1016/j.jcyt.2015.03.003
更新日期:2015-06-01 00:00:00
abstract:BACKGROUND AIMS:To date there are only very few data available on the ligamentogenic differentiation capacity of mesenchymal stromal/progenitor cells (MSC) and anterior cruciate ligament (ACL) fibroblasts. METHODS:We describe the in vitro potential of MSC and ACL cells to undergo ligamentogenic differentiation upon tr...
journal_title:Cytotherapy
pub_type: 杂志文章
doi:10.3109/14653241003709652
更新日期:2010-07-01 00:00:00
abstract:BACKGROUND:The efficiency of chimeric antigen receptor (CAR) T-cell-based therapies depends on a sufficient expansion of CAR T cells in vivo and can be weakened by intra-tumoral suppression of CAR T cell functions, leading to a failure of therapy. For example, certain B-cell malignancies such as chronic lymphocytic leu...
journal_title:Cytotherapy
pub_type: 杂志文章
doi:10.1016/j.jcyt.2020.08.005
更新日期:2020-12-01 00:00:00
abstract:BACKGROUND AIMS:To assess the impacts of stem cell therapy on clinical outcomes in patients with non-ischemic cardiomyopathy (NICM). The effect of stem cell therapy on prognosis is unclear and controversial. METHODS:The authors performed a systematic review and meta-analysis of the effects of autologous stem cell tran...
journal_title:Cytotherapy
pub_type: 杂志文章,评审
doi:10.1016/j.jcyt.2020.06.006
更新日期:2020-12-01 00:00:00
abstract:BACKGROUND AIMS:Retinal degeneration (RD) is an inherited eye disease characterized by irreversible photoreceptor loss. Conventionally, the activation of the resident microglia is secondary to the disease. Stem cell-based therapy has recently made rapid progress in treating RD. Although it has been demonstrated that th...
journal_title:Cytotherapy
pub_type: 杂志文章
doi:10.1016/j.jcyt.2016.03.001
更新日期:2016-06-01 00:00:00
abstract:BACKGROUND AIMS:Our previous study has demonstrated the stem cell-like properties of human umbilical cord lining epithelial cells (CLECs) and their capability for epidermal reconstitution in organotypic skin culture; however, the immunogenicity of these cells has not been clearly defined. We assessed several aspects of...
journal_title:Cytotherapy
pub_type: 杂志文章
doi:10.1016/j.jcyt.2013.10.008
更新日期:2014-05-01 00:00:00