Abstract:
BACKGROUND:Hematopoietic progenitor cells (HPC) circulate at high levels at birth and disappear rapidly afterwards, but the underlying mechanism it is not known. The aim of this study was to assess circulating HPC in cord blood at different gestational ages and shortly after birth and concomitantly study the biologic markers involved in this phenomenon. METHODS:All samples were analyzed for CD34(+) cells, colony-forming units (CFU) and cytokines. RESULTS:The results obtained confirmed a slight decrease in HPC concentration during the late stage of fetal life (R(2)=0.41). After birth, CD34(+) cells showed a rapid decline from circulation: 25+/-29% at 3 h, 51+/-42% at 12 h and 80+/-48% at 60 h. CFU cleared following a similar pattern. Cord plasma showed higher concentrations of stem cell factor (SCF), fetal liver tyrosine kinase 3-ligand (FLT3l), erythrpoietin (EPO), granulocyte colony-stimulating factor (G-CSF) and interleukin-11 (IL-11) compared with an adult control. Interestingly, the EPO concentration in newborn plasma correlated with the kinetics of HPC decline after birth. Moreover, we observed an up-regulation of l-selectin and a down-regulation of CXCR4 expression in CD34(+) cells 3 h after birth. DISCUSSION:These data combined suggest that an active homing process results in the clearance of HPC from the circulation immediately after birth.
journal_name
Cytotherapyjournal_title
Cytotherapyauthors
Gonzalez S,Amat L,Azqueta C,Madrigal JA,Laïlla JM,Garcia J,Querol Sdoi
10.1080/14653240802499148subject
Has Abstractpub_date
2009-01-01 00:00:00pages
35-42issue
1eissn
1465-3249issn
1477-2566pii
905997079journal_volume
11pub_type
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