Abstract:
RATIONALE AND OBJECTIVES:The aim of this study was to evaluate the intratumoral residence time of microbubbles (MBs) targeted to alpha(v)beta(3) integrin expressed in the endothelial cells of mice during the process of tumor angiogenesis. MATERIALS AND METHODS:For the preparation of MBs, decafluorobutane gas was sonically dispersed in phosphate buffer saline containing L-A-phosphatidylcholine-distearoyl, polyethylene glycol 40 stearate, and 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[biotinyl(polyethylene glycol)2000] in a 77:15:8 molar ratio. Avidin-fluorescein isothiocyanate and biotin-cyclic arginine-glycine-aspartate-D-tyrosine-lysine (cRGD) or biotin-alanine-glycine-aspartate (AGD) conjugates were added to the reaction mixture. Adhesion testing of the targeting MBs was performed for the MS-1 cell line expressing alpha(v)beta(3) integrin in vitro. The in vivo acoustic properties of the MBs were assessed by clinical ultrasound on the HT1080 fibrosarcoma model (n = 8) for 1 hour. Cryosections were stained with hematoxylin and eosin and by immunohistochemical staining to identify expression of alpha(v)beta(3) integrin in the HT1080 tumor. RESULTS:The adherence of the MBs conjugated to cRGD was significantly greater than the adherence of the MBs conjugated to biotin-AGD (P < .01) for the MS-1 endothelial cell line. The acoustic enhancement on ultrasound was observed as a stable imaging window until 1 hour after injection of the MB conjugates in the mice. The MBs targeted via cRGD preferentially adhered to the vascular endothelium of the HT-1080 tumors. The findings of ultrasound imaging were correlated with immunohistochemical findings for the expression of alpha(v)beta(3) integrin on the vascular endothelium of the tumors. CONCLUSIONS:The prepared MBs conjugated with cRGD demonstrated a sufficient residence time to attach to the target integrin of tumor tissues. This finding suggests that the MBs are a potential molecular contrast agent that enables characterization of tumor angiogenesis and the monitoring of antitumor and antiangiogenic therapy.
journal_name
Acad Radioljournal_title
Academic radiologyauthors
Jun HY,Park SH,Kim HS,Yoon KHdoi
10.1016/j.acra.2009.07.017subject
Has Abstractpub_date
2010-01-01 00:00:00pages
54-60issue
1eissn
1076-6332issn
1878-4046pii
S1076-6332(09)00415-2journal_volume
17pub_type
杂志文章abstract:RATIONALE AND OBJECTIVES:Amphiphilic biocompatible polyoxyethylene (PEO)-based polymers form particles (micelles) that are 10-50 nm in diameter. In the current research, we successfully incorporated amphiphilic indium-111 (111In) and gadolinium chelates into these particles and used them as particulate contrast media i...
journal_title:Academic radiology
pub_type: 杂志文章
doi:10.1016/s1076-6332(96)80448-x
更新日期:1996-03-01 00:00:00
abstract:RATIONALE AND OBJECTIVES:The aim of this study was to determine the optimal arterial phase delay for computed tomography imaging of hepatocellular carcinoma (HCC) before and after transarterial chemoembolization (TACE) using a low iodine dose protocol. MATERIALS AND METHODS:A total of 39 patients with known HCC were i...
journal_title:Academic radiology
pub_type: 杂志文章
doi:10.1016/j.acra.2015.08.021
更新日期:2015-12-01 00:00:00
abstract:RATIONALE AND OBJECTIVES:Renal tubular vacuolization (RTV), which has been shown to occur after the use of iodinated contrast material, may be one of the earliest signs of contrast medium-induced renal injury. In this study, the authors tested a method for preventing RTV with the administration of acetylcysteine, theop...
journal_title:Academic radiology
pub_type: 杂志文章
doi:10.1016/s1076-6332(03)80061-2
更新日期:2003-05-01 00:00:00
abstract:RATIONALE AND OBJECTIVES:The aim of this study was to prospectively investigate the diagnostic value of triple rule-out computed tomography (CT) in patients suspected of having acute pulmonary embolism (PE). MATERIALS AND METHODS:A total of 125 patients with suspicion of PE, of whom 14 patients had the additional clin...
journal_title:Academic radiology
pub_type: 杂志文章
doi:10.1016/j.acra.2009.01.014
更新日期:2009-06-01 00:00:00
abstract:RATIONALE AND OBJECTIVES:A novel ventilation imaging method based on four-dimensional (4D) computed tomography (CT) has been applied to the field of radiation oncology. Understanding its reproducibility is a prerequisite for clinical applications. The purpose of this study was to quantify the reproducibility of 4D CT v...
journal_title:Academic radiology
pub_type: 杂志文章
doi:10.1016/j.acra.2012.07.006
更新日期:2012-12-01 00:00:00
abstract:RATIONALE AND OBJECTIVES:We sought to evaluate the diagnostic performance of an artificial neural network (ANN) and binary logistic regression (BLR) in differentiating malignant from benign thyroid nodules on ultrasonography. MATERIALS AND METHODS:Two experienced radiologists, who were unaware of the histopathological...
journal_title:Academic radiology
pub_type: 杂志文章
doi:10.1016/j.acra.2007.12.022
更新日期:2008-07-01 00:00:00
abstract:RATIONALE AND OBJECTIVES:The aim of this study was to compare the performance of various image-based metrics computed from thoracic high-resolution computed tomography (HRCT) with data from pulmonary function testing (PFT) in characterizing interstitial lung disease (ILD) and chronic obstructive pulmonary disease (COPD...
journal_title:Academic radiology
pub_type: 杂志文章
doi:10.1016/j.acra.2012.03.007
更新日期:2012-07-01 00:00:00
abstract:RATIONALE AND OBJECTIVES:The aim of this study was to retrospectively evaluate an automated global scoring system for evaluating the extent and severity of disease in a known cohort of patients with documented bronchiectasis. On the basis of a combination of validated three-dimensional automated algorithms for bronchia...
journal_title:Academic radiology
pub_type: 杂志文章
doi:10.1016/j.acra.2010.04.019
更新日期:2010-09-01 00:00:00
abstract:RATIONALE AND OBJECTIVES:To determine the impact of additional coronary calcium scoring on total effective radiation dose and diagnostic accuracy of low-dose computed tomography coronary angiography (CTCA) with prospective electrocardiogram (ECG) triggering. MATERIALS AND METHODS:Sixty-one consecutive patients underwe...
journal_title:Academic radiology
pub_type: 杂志文章
doi:10.1016/j.acra.2009.09.007
更新日期:2010-02-01 00:00:00
abstract:RATIONALE AND OBJECTIVES:To evaluate the feasibility and performance of an x-ray beam equalization system for chest radiography using anthropomorphic phantoms. MATERIALS AND METHODS:Area beam equalization involves the process of the initial unequalized image acquisition, attenuator thickness calculation, mask generati...
journal_title:Academic radiology
pub_type: 杂志文章
doi:10.1016/s1076-6332(03)00730-x
更新日期:2004-04-01 00:00:00
abstract:RATIONALE AND OBJECTIVES:We introduce a new approach to the prediction of final infarct growth in human acute ischemic stroke based on image analysis of the apparent diffusion coefficient (ADC) maps obtained from magnetic resonance imaging. Evidence from multiple previous studies indicate that ADC maps are likely to re...
journal_title:Academic radiology
pub_type: 杂志文章
doi:10.1016/j.acra.2007.07.007
更新日期:2008-01-01 00:00:00
abstract:RATIONALE AND OBJECTIVES:The study aimed to determine if intrathoracic fat volumes are associated with the presence and severity of systemic sclerosis (SSc), defined by the presence of pulmonary arterial hypertension (PAH). MATERIALS AND METHODS:A total of 265 patients were included in the study, 202 of whom had SSc (...
journal_title:Academic radiology
pub_type: 杂志文章
doi:10.1016/j.acra.2017.07.003
更新日期:2017-12-01 00:00:00
abstract:RATIONALE AND OBJECTIVES:In assessing diagnostic accuracy it is often essential to determine the reader's ability both to detect and to correctly locate multiple abnormalities per patient. The authors developed a new approach for the detection and localization of multiple abnormalities and compared it with other approa...
journal_title:Academic radiology
pub_type: 杂志文章
doi:10.1016/s1076-6332(00)80324-4
更新日期:2000-07-01 00:00:00
abstract:RATIONALE AND OBJECTIVES:The authors evaluated changes of lung attenuation in pigs, with special attention to the mosaic pattern of low attenuation, at thin-section computed tomography (CT) after obstruction of the proximal pulmonary artery with a detachable balloon. MATERIALS AND METHODS:In seven pigs, nine sites of ...
journal_title:Academic radiology
pub_type: 杂志文章
doi:10.1016/s1076-6332(98)80241-9
更新日期:1998-12-01 00:00:00
abstract:RATIONALE AND OBJECTIVES:Digital chest imaging has replaced film chest radiographs in many centers, but the International Labour Organization classification system, which is the most widely used system for recognition and classification of dust-related abnormalities, is predicated on film chest radiographs. The purpose...
journal_title:Academic radiology
pub_type: 杂志文章
doi:10.1016/j.acra.2008.12.020
更新日期:2009-06-01 00:00:00
abstract:RATIONALE AND OBJECTIVES:Pulmonary atelectasis presumably promotes and facilitates lung injury. However, data are limited on its direct and remote relation to inflammation. We aimed to assess regional 2-deoxy-2-[18F]-fluoro-D-glucose (18F-FDG) kinetics representative of inflammation in atelectatic and normally aerated ...
journal_title:Academic radiology
pub_type: 杂志文章
doi:10.1016/j.acra.2019.12.022
更新日期:2020-12-01 00:00:00
abstract:RATIONALE AND OBJECTIVES:To determine whether quantitative dynamic contrast-enhanced (DCE) and diffusion-weighted (DW) magnetic resonance imaging (MRI) features can discriminate malignant from benign axillary lymph nodes (ALNs) identified as suspicious on clinical breast MRI in patients newly diagnosed with breast canc...
journal_title:Academic radiology
pub_type: 杂志文章
doi:10.1016/j.acra.2014.10.010
更新日期:2015-04-01 00:00:00
abstract:RATIONALE AND OBJECTIVES:Peritumoral features have been suggested to be useful in improving the prediction performance of radiomic models. The aim of this study is to systematically investigate the prediction performance improvement for sentinel lymph node (SLN) status in breast cancer from peritumoral features in radi...
journal_title:Academic radiology
pub_type: 杂志文章
doi:10.1016/j.acra.2020.10.015
更新日期:2020-11-04 00:00:00
abstract:RATIONALE AND OBJECTIVES:To determine whether an unenhanced low-dose image acquired during automated contrast bolus timing can be used to assess hepatic steatosis. MATERIALS AND METHODS:Fifty subjects (29 male, 21 female; 26-92 years; mean body mass index (BMI; 26.9) with abdominal multiphasic computed tomography were...
journal_title:Academic radiology
pub_type: 杂志文章
doi:10.1016/j.acra.2011.02.013
更新日期:2011-07-01 00:00:00
abstract:RATIONALE AND OBJECTIVES:There is discordance between the American Board of Radiology (ABR) and many radiology trainees with respect to the most appropriate means to prepare for the ABR Core Examination. Whereas the ABR suggests that participation in routine clinical examination interpretation best prepares a trainee f...
journal_title:Academic radiology
pub_type: 杂志文章
doi:10.1016/j.acra.2019.10.004
更新日期:2020-02-01 00:00:00
abstract:RATIONALE AND OBJECTIVES:The aim of this study was to identify clinically useful tumor/normal brain apparent diffusion coefficient (ADC) ratios for distinguishing common pediatric cerebellar tumors. MATERIALS AND METHODS:Review of medical records revealed 79 patients with cerebellar tumors who underwent preoperative m...
journal_title:Academic radiology
pub_type: 杂志文章
doi:10.1016/j.acra.2012.03.004
更新日期:2012-07-01 00:00:00
abstract:RATIONALE AND OBJECTIVES:The authors evaluated whether fast spectroscopic imaging of water and fat resonances can produce high-quality anatomic magnetic resonance (MR) images of rodent tumors and human breast. MATERIALS AND METHODS:Fast MR spectroscopic images of eight rats with mammary tumors were acquired by using a...
journal_title:Academic radiology
pub_type: 杂志文章
doi:10.1016/s1076-6332(98)80226-2
更新日期:1998-04-01 00:00:00
abstract:RATIONALE AND OBJECTIVES:The authors performed this study to compare magnetic resonance (MR) venography and conventional venography in the diagnosis of deep venous thrombosis (DVT) in the calf after sonography. MATERIALS AND METHODS:Sonography was performed in 595 patients who were suspected of having lower-extremity ...
journal_title:Academic radiology
pub_type: 临床试验,杂志文章
doi:10.1016/s1076-6332(03)80764-x
更新日期:2001-09-01 00:00:00
abstract:RATIONALE AND OBJECTIVES:To retrospectively analyze the significance of 3.0-T contrast-enhanced (CE) magnetic resonance angiography (MRA) with calf compression in the lower limbs of diabetic patients with peripheral vascular disease. MATERIALS AND METHODS:Sixty-one type 2 diabetes patients underwent both MRA and digit...
journal_title:Academic radiology
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.acra.2011.01.013
更新日期:2011-06-01 00:00:00
abstract:RATIONALE AND OBJECTIVES:The goal of this prospective, interinstitutional study was to compare the long-term instructional effectiveness of a pediatric multimedia textbook (MMTB) to that of a standard lecture and a printed textbook. MATERIALS AND METHODS:A randomized cohort of 89 3rd-year medical students from two ins...
journal_title:Academic radiology
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1016/s1076-6332(97)80074-8
更新日期:1997-11-01 00:00:00
abstract:RATIONALE AND OBJECTIVES:Breast segmentation using the U-net architecture was implemented and tested in independent validation datasets to quantify fibroglandular tissue volume in breast MRI. MATERIALS AND METHODS:Two datasets were used. The training set was MRI of 286 patients with unilateral breast cancer. The segme...
journal_title:Academic radiology
pub_type: 杂志文章
doi:10.1016/j.acra.2019.01.012
更新日期:2019-11-01 00:00:00
abstract:RATIONALE AND OBJECTIVES:The aim of the study is to gauge radiology fellowship directors' experiences with the fellowship application process and perceptions of the National Resident Matching Program Radiology Fellowship Match, as well as compare these perceptions with those of senior residents. METHODS AND MATERIALS:...
journal_title:Academic radiology
pub_type: 杂志文章
doi:10.1016/j.acra.2005.09.085
更新日期:2006-01-01 00:00:00
abstract:RATIONALE AND OBJECTIVES:The capability of wavelet transforms to separate signals into frequency bands is the basis for its use in image compression and storage, data management and transmission, and, recently, extraction of latent images of tissue components from noisy medical images. Analysis of temporal variations o...
journal_title:Academic radiology
pub_type: 杂志文章
doi:10.1016/j.acra.2007.06.018
更新日期:2007-12-01 00:00:00
abstract:RATIONALE AND OBJECTIVES:The purpose of this study was to determine the feasibility of using transabdominal ultrasonography (US) to monitor tumor growth and response to therapy in a mouse model of orthotopic bladder carcinoma. MATERIALS AND METHODS:Human bladder carcinoma cell suspensions were injected into the bladde...
journal_title:Academic radiology
pub_type: 杂志文章
doi:10.1016/s1076-6332(01)90038-8
更新日期:2001-02-01 00:00:00
abstract:RATIONALE AND OBJECTIVES:Skeletal muscle mass measurement is the most important element for diagnosing sarcopenia. MRI has an excellent soft-tissue contrast, which can non-invasively assess abdominal skeletal muscle area (SMA) as well as CT. This study aimed to assess the validity and reliability of abdominal SMA measu...
journal_title:Academic radiology
pub_type: 杂志文章
doi:10.1016/j.acra.2020.09.013
更新日期:2020-10-28 00:00:00