Mucin gene expression in bile of patients with and without gallstone disease, collected by endoscopic retrograde cholangiography.

Abstract:

AIM:To investigate the pattern of mucin expression and concentration in bile obtained during endoscopic retrograde cholangiography (ERC) in relation to gallstone disease. METHODS:Bile samples obtained at ERC from 29 consecutive patients, 17 with and 12 without gallstone disease were evaluated for mucin content by gel filtration on a Sepharose CL-4B column. Dot blot analysis for bile mucin apoproteins was performed with antibodies to Mucin 1 (MUC1), MUC2, MUC3, MUC5AC, MUC5B and MUC6. Staining intensity score (0-3) was used as a measure of antigen expression. RESULTS:MUC1, MUC2, MUC3, MUC5AC, MUC5B and MUC6 were demonstrated in 34.4%, 34.4%, 51.7%, 51.7%, 55.1% and 27.5% of bile samples, respectively. The staining intensity scores were 0.62 +/- 0.94, 0.58 +/- 0.90, 0.79 +/- 0.97, 1.06 +/- 1.22, 1.20 +/- 1.26 and 0.41 +/- 0.73, respectively. Mean mucin concentration measured in bile by the Sepharose CL-4B method was 22.8 +/- 24.0 mg/mL (range 3.4-89.0 mg/mL). Mean protein concentration was 8.1 +/- 4.8 mg/mL (range 1.7-23.2 mg/mL). CONCLUSION:High levels of MUC3, MUC5AC and MUC5B are expressed in bile aspirated during ERC examination. A specific pattern of mucin gene expression or change in mucin concentration was not found in gallstone disease.

journal_name

World J Gastroenterol

authors

Vilkin A,Geller A,Levi Z,Niv Y

doi

10.3748/wjg.15.2367

subject

Has Abstract

pub_date

2009-05-21 00:00:00

pages

2367-71

issue

19

eissn

1007-9327

issn

2219-2840

journal_volume

15

pub_type

杂志文章
  • Portal vein embolization induces compensatory hypertrophy of remnant liver.

    abstract:AIM:To evaluate the effectiveness and safety of different portal vein branch embolization agents in inducing compensatory hypertrophy of the remnant liver and to offer a theoretic basis for clinical portal vein branch embolization. METHODS:Forty-one adult dogs were included in the experiment and divided into four grou...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3748/wjg.v12.i3.408

    authors: Huang JY,Yang WZ,Li JJ,Jiang N,Zheng QB

    更新日期:2006-01-21 00:00:00

  • Association between obesity-related adipokines and colorectal cancer: a case-control study and meta-analysis.

    abstract:AIM:To examine the association between obesity-related adipokines (adiponectin, leptin, resistin, interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α) and colorectal cancer (CRC) risk. METHODS:Serum levels of adipokines were measured in 100 CRC patients and age- and sex-matched controls for the data analysis. Unc...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章,meta分析,评审

    doi:10.3748/wjg.v20.i24.7941

    authors: Joshi RK,Kim WJ,Lee SA

    更新日期:2014-06-28 00:00:00

  • Levels of matrix metalloproteinase-1 and tissue inhibitors of metalloproteinase-1 in gastric cancer.

    abstract:AIM:To evaluate the levels of preoperative serum matrix metalloproteinase-1 (MMP-1) and tissue inhibitor of metalloproteinase-1 (TIMP-1) in gastric cancer. METHODS:One hundred gastric cancer patients who underwent gastrectomy were enrolled in this study. The serum concentrations of MMP-1 and TIMP-1 in these patients a...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3748/wjg.v17.i16.2109

    authors: Kemik O,Kemik AS,Sümer A,Dulger AC,Adas M,Begenik H,Hasirci I,Yilmaz O,Purisa S,Kisli E,Tuzun S,Kotan C

    更新日期:2011-04-28 00:00:00

  • Post-gastrectomy patients need to be followed up for 20-30 years.

    abstract::AIM:To investigate the incidence and management of nutritional deficiencies following a gastrectomy.METHODS:A gastrectomy population of 227 patients in London was followed up for 30 years after operation to detect and treat nutritional deficiencies.RESULTS:By the end of the first decade iron deficiency was the commone...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3748/wjg.v6.i1.45

    authors: Tovey FI,Hobsley M

    更新日期:2000-02-01 00:00:00

  • Effects of progesterone on gastric emptying and intestinal transit in male rats.

    abstract:AIM:To study the dose-dependent of progesterone (P) effect and the interaction between the oxytocin (OT) and P on gastrointestinal motility. METHODS:In order to monitor the gastric emptying and intestinal transit, the SD male rats were intubated via a catheter with normal saline (3 ml/kg) containing Na(2)(51)CrO(4) (0...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3748/wjg.v8.i2.338

    authors: Liu CY,Chen LB,Liu PY,Xie DP,Wang PS

    更新日期:2002-04-01 00:00:00

  • Bacterial flora concurrent with Helicobacter pylori in the stomach of patients with upper gastrointestinal diseases.

    abstract:AIM:To investigate the non-Helicobacter pylori (H. pylori) bacterial flora concurrent with H. pylori infection. METHODS:A total of 103 gastric biopsy specimens from H. pylori positive patients were selected for bacterial culture. All the non-H. pylori bacterial isolates were identified by matrix-assisted laser desorpt...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3748/wjg.v18.i11.1257

    authors: Hu Y,He LH,Xiao D,Liu GD,Gu YX,Tao XX,Zhang JZ

    更新日期:2012-03-21 00:00:00

  • Effect of fluoxetine on depression-induced changes in the expression of vasoactive intestinal polypeptide and corticotrophin releasing factor in rat duodenum.

    abstract:AIM:To investigate changes in vasoactive intestinal polypeptide (VIP) and corticotrophin releasing factor (CRF) in the plasma and duodenum of chronic stress-induced depressed rats and the effects of fluoxetine hydrochloride (fluoxetine) treatment on depression-induced changes in VIP and CRF. METHODS:A Sprague-Dawley r...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3748/wjg.v13.45.6060

    authors: Huang YL,Yu JP,Wang GH,Chen ZH,Wang Q,Xiao L

    更新日期:2007-12-07 00:00:00

  • Effect of p27(KIP1) on cell cycle and apoptosis in gastric cancer cells.

    abstract:AIM:To elucidate the effect of p27(KIP1) on cell cycle and apoptosis regulation in gastric carcinoma cells. METHODS:The whole length of p27(KIP1) cDNA was transfected into human gastric cancer cell line SCG7901 by lipofectamine. Expression of p27(KIP1) protein or mRNA was analyzed by Western blot and RNA dot blotting,...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3748/wjg.v11.i45.7072

    authors: Zheng JY,Wang WZ,Li KZ,Guan WX,Yan W

    更新日期:2005-12-07 00:00:00

  • Comparison of two different laparotomy methods for modeling rabbit VX2 hepatocarcinoma.

    abstract:AIM:To compare two different laparotomy methods for modeling rabbit VX2 hepatocarcinoma. METHODS:Thirty New Zealand rabbits were randomly divided into two groups: A and B. Group A was assigned a traditional laparotomy method (embedding tumor fragments directly into the liver with tweezers). Group B was subjected to an...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3748/wjg.v21.i16.4875

    authors: Chen Z,Kang Z,Xiao EH,Tong M,Xiao YD,Li HB

    更新日期:2015-04-28 00:00:00

  • Retrospective analysis of extra-gastrointestinal stromal tumors.

    abstract:AIM:To investigate the clinicopathologic features of patients with extra-gastrointestinal stromal tumors (EGISTs) in South Korea. METHODS:A total of 51 patients with an EGIST were identified. The clinicopathologic features, including sex, age, location, tumor size, histology, mitotic rate, immunohistochemical features...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3748/wjg.v21.i6.1845

    authors: Yi JH,Park BB,Kang JH,Hwang IG,Shin DB,Sym SJ,Ahn HK,Lee SI,Lim DH,Park KW,Won YW,Lim SH,Park SH

    更新日期:2015-02-14 00:00:00

  • Endoscopic ultrasound-guided treatments: are we getting evidence based--a systematic review.

    abstract::The continued need to develop less invasive alternatives to surgical and radiologic interventions has driven the development of endoscopic ultrasound (EUS)-guided treatments. These include EUS-guided drainage of pancreatic fluid collections, EUS-guided necrosectomy, EUS-guided cholangiography and biliary drainage, EUS...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章,评审

    doi:10.3748/wjg.v20.i26.8424

    authors: Fabbri C,Luigiano C,Lisotti A,Cennamo V,Virgilio C,Caletti G,Fusaroli P

    更新日期:2014-07-14 00:00:00

  • Colorectal cancer in patients with inflammatory bowel disease: can we predict risk?

    abstract::The inflammatory bowel diseases (IBD), Crohn's disease (CD) and ulcerative colitis (UC), may be complicated by colorectal cancer (CRC). In a recent population-based cohort study of 47 347 Danish patients with IBD by Tine Jess and colleagues 268 patients with UC and 70 patients with CD developed CRC during 30 years of ...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3748/wjg.v18.i31.4091

    authors: Andersen V,Halfvarson J,Vogel U

    更新日期:2012-08-21 00:00:00

  • Diabetes mellitus, insulin resistance and hepatitis C virus infection: A contemporary review.

    abstract:AIM:To summarise the literature data on hepatitis C virus (HCV)-infected patients concerning the prevalence of glucose abnormalities and associated risk. METHODS:We conducted a PubMed search and selected all studies found with the key words "HCV" or "hepatitis C virus" and "diabetes" or "insulin resistance". We includ...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章,评审

    doi:10.3748/wjg.v23.i9.1697

    authors: Desbois AC,Cacoub P

    更新日期:2017-03-07 00:00:00

  • Current hurdles in the management of eosinophilic oesophagitis: the next steps.

    abstract::Eosinophilic oesophagitis (EoE) is a chronic, antigen mediated disease of the disease of the oesophagus that may present in both adults and children. It is characterised by intermittent dysphagia, food bolus obstruction and weight loss. The pathogenesis is incompletely understood but is thought to culminate in poor co...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3748/wjg.v19.i6.790

    authors: Attwood SE,Wilson MS

    更新日期:2013-02-14 00:00:00

  • Haemoglobin, albumin, lymphocyte and platelet predicts postoperative survival in pancreatic cancer.

    abstract:BACKGROUND:Systemic inflammation and nutrition status play an important role in cancer metastasis. The combined index of hemoglobin, albumin, lymphocyte, and platelet (HALP), consisting of haemoglobin, albumin, lymphocytes, and platelets, is considered as a novel marker to reflect both systemic inflammation and nutriti...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3748/wjg.v26.i8.828

    authors: Xu SS,Li S,Xu HX,Li H,Wu CT,Wang WQ,Gao HL,Jiang W,Zhang WH,Li TJ,Ni QX,Liu L,Yu XJ

    更新日期:2020-02-28 00:00:00

  • The prognostic significance of clinical and pathological features in hepatocellular carcinoma.

    abstract::The prognosis of patients with HCC still remains dismal. The life expectancy of HCC patients is hard to predict because of the high possibility of postoperative recurrence. Many factors, such as patient's general conditions, macroscopic tumor morphology, as well as tumor histopathology features, have been proven of pr...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章,评审

    doi:10.3748/wjg.v8.i2.193

    authors: Qin LX,Tang ZY

    更新日期:2002-04-01 00:00:00

  • Solubility of disulfide-bonded proteins in the cytoplasm of Escherichia coli and its "oxidizing" mutant.

    abstract:AIM:To study the influence of redox environment of Escherichia coli (E. coli) cytoplasm on disulfide bond formation of recombinant proteins. METHODS:Bovine fibroblast growth factor (BbFGF) was selected as a model of simple proteins with a single disulfide bond and free cysteines. Anti-HBsAg single-chain Fv (HBscFv), a...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3748/wjg.v11.i7.1077

    authors: Xiong S,Wang YF,Ren XR,Li B,Zhang MY,Luo Y,Zhang L,Xie QL,Su KY

    更新日期:2005-02-21 00:00:00

  • Parameters of a severe disease course in ulcerative colitis.

    abstract:AIM:To detect high risk patients with a progressive disease course of ulcerative colitis (UC) requiring immunosuppressive therapy (IT). METHODS:A retrospective, multicenter analysis of 262 UC patients from eight German tertiary inflammatory bowel disease centres was performed. Patients were divided into two groups dep...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章,多中心研究

    doi:10.3748/wjg.v20.i35.12574

    authors: Stallmach A,Nickel L,Lehmann T,Bokemeyer B,Bürger M,Hüppe D,Kruis W,Nikolaus S,Preiss JC,Sturm A,Teich N,Schmidt C

    更新日期:2014-09-21 00:00:00

  • Liver myofibroblasts activate protein C and respond to activated protein C.

    abstract:AIM:To study the protein C activation system in human liver myofibroblasts, and the effects of activated protein C (APC) on these cells. METHODS:Human liver myofibroblasts were obtained by outgrowth. Expression of protease activated receptor 1 (PAR-1), endothelial protein C receptor (EPCR) and thrombomodulin (TM) was ...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3748/wjg.v16.i2.210

    authors: Gillibert-Duplantier J,Rullier A,Neaud V,Kisiel W,Rosenbaum J

    更新日期:2010-01-14 00:00:00

  • Resveratrol and fenofibrate ameliorate fructose-induced nonalcoholic steatohepatitis by modulation of genes expression.

    abstract:AIM:To evaluate the effect of resveratrol, alone and in combination with fenofibrate, on fructose-induced metabolic genes abnormalities in rats. METHODS:Giving a fructose-enriched diet (FED) to rats for 12 wk was used as a model for inducing hepatic dyslipidemia and insulin resistance. Adult male albino rats (150-200 ...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3748/wjg.v22.i10.2931

    authors: Abd El-Haleim EA,Bahgat AK,Saleh S

    更新日期:2016-03-14 00:00:00

  • Coexistence of small cell neuroendocrine carcinoma and villous adenoma in the ampulla of Vater.

    abstract::Small cell neuroendocrine carcinoma of the ampulla of Vater is extremely rare and different from the common ampullary adenocarcinoma. The ampullary adenoma is also a rare neoplasm and has the potential to develop an adenocarcinoma. Their coexistence has been rarely reported in the literature. We herein describe an unu...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3748/wjg.14.4709

    authors: Sun JH,Chao M,Zhang SZ,Zhang GQ,Li B,Wu JJ

    更新日期:2008-08-07 00:00:00

  • Role of MGST1 in reactive intermediate-induced injury.

    abstract::Microsomal glutathione transferase (MGST1, EC 2.5.1.18) is a membrane bound glutathione transferase extensively studied for its ability to detoxify reactive intermediates, including metabolic electrophile intermediates and lipophilic hydroperoxides through its glutathione dependent transferase and peroxidase activitie...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章,评审

    doi:10.3748/wjg.v17.i20.2552

    authors: Schaffert CS

    更新日期:2011-05-28 00:00:00

  • Efficacy profiles for different concentrations of Lactobacillus acidophilus in experimental colitis.

    abstract:AIM:To determine the efficacy profiles of different concentrations of Lactobacillus acidophilus (L. acidophilus) for treating colitis using an experimental murine model. METHODS:Colitis was established in 64 BALB/c mice by adding 5% dextran sodium sulfate (DSS) to the drinking water and allowing ad libitum access for ...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3748/wjg.v19.i32.5347

    authors: Chen LL,Zou YY,Lu FG,Li FJ,Lian GH

    更新日期:2013-08-28 00:00:00

  • Lower body weight and female gender: hyperphosphatemia risk factors after sodium phosphate preparations.

    abstract::Casais et al have reported an inverse correlation between serum phosphate and body weight after administration of sodium phosphate at a dose of 60 g. Our group has already described the relationship between body weight and hyperphosphatemia with these preparations, although our study was not quoted by Casais. We perfo...

    journal_title:World journal of gastroenterology

    pub_type: 评论,信件

    doi:10.3748/wjg.v17.i21.2681

    authors: Deepak P,Ehrenpreis ED

    更新日期:2011-06-07 00:00:00

  • Outcomes of simple saline-coupled bipolar electrocautery for hepatic resection.

    abstract:AIM:To evaluate the application of bipolar coagulation (BIP) in hepatectomy by comparing the efficacy of BIP alone, cavitron ultrasonic surgical aspirator (CUSA) + BIP and conventional clamp crushing (CLAMP). METHODS:Based on our database of patient records, a total of 380 consecutive patients who underwent hepatectom...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3748/wjg.v20.i26.8638

    authors: Guo JY,Li DW,Liao R,Huang P,Kong XB,Wang JM,Wang HL,Luo SQ,Yan X,Du CY

    更新日期:2014-07-14 00:00:00

  • Overexpression of insulin-like growth factor-I receptor as a pertinent biomarker for hepatocytes malignant transformation.

    abstract:AIM:To investigate the dynamic features of insulin-like growth factor-I receptor (IGF-IR) expression in rat hepatocarcinogenesis, and the relationship between IGF-IR and hepatocytes malignant transformation at mRNA or protein level. METHODS:Hepatoma models were made by inducing with 2-fluorenylacetamide (2-FAA) on mal...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3748/wjg.v19.i36.6084

    authors: Yan XD,Yao M,Wang L,Zhang HJ,Yan MJ,Gu X,Shi Y,Chen J,Dong ZZ,Yao DF

    更新日期:2013-09-28 00:00:00

  • A new framework for reverse cholesterol transport: non-biliary contributions to reverse cholesterol transport.

    abstract::Reduction of low-density lipoprotein-cholesterol through statin therapy has only modestly decreased coronary heart disease (CHD)-associated mortality in developed countries, which has prompted the search for alternative therapeutic strategies for CHD. Major efforts are now focused on therapies that augment high-densit...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章,评审

    doi:10.3748/wjg.v16.i47.5946

    authors: Temel RE,Brown JM

    更新日期:2010-12-21 00:00:00

  • Nuclear and mitochondrial DNA microsatellite instability in hepatocellular carcinoma in Chinese.

    abstract:AIM:To study the nuclear microsatellite instability (nMSI) at BAT26 and mitochondrial microsatellite instability (mtMSI) in the occurrence and development of hepatocellular carcinoma and the relationship between nMSI and mtMSI. METHODS:nMSI was observed with PCR and mtMSI with PCR-SSCP in 52 cases of hepatocellular ca...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3748/wjg.v10.i3.371

    authors: Fang DC,Fang L,Wang RQ,Yang SM

    更新日期:2004-02-01 00:00:00

  • Methotrexate induced sprue-like syndrome.

    abstract::A 52 year-old male patient diagnosed of ankylosing spondylitis presented with an iron deficiency anemia after a ten-month treatment of methotrexate. He did not respond to treatment with oral iron not a proton pump inhibitor and an upper endoscopy was performed. The histological study of the duodenal biopsies showed vi...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3748/wjg.14.7009

    authors: Boscá MM,Añón R,Mayordomo E,Villagrasa R,Balza N,Amorós C,Corts JR,Benages A

    更新日期:2008-12-07 00:00:00

  • Impact of postoperative glycemic control and nutritional status on clinical outcomes after total pancreatectomy.

    abstract:AIM:To evaluate the impact of glycemic control and nutritional status after total pancreatectomy (TP) on complications, tumor recurrence and overall survival. METHODS:Retrospective records of 52 patients with pancreatic tumors who underwent TP were collected from 2007 to 2015. A series of clinical parameters collected...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3748/wjg.v23.i2.265

    authors: Shi HJ,Jin C,Fu DL

    更新日期:2017-01-14 00:00:00