Genotype analysis of hepatitis delta virus from hepatitis B surface antigen-positive patients using PCR-RFLP in Tehran, Iran.

Abstract:

BACKGROUND:Hepatitis delta virus is a unique human pathogen responsible for some 20 million infections globally. This virus is dependent on hepatitis B virus for transmission and propagation. Currently, at least three genotypes of hepatitis delta virus with different geographic distribution and clinical manifestations are described. METHODS:In this study, hepatitis delta virus RNA of 35 patients' sera were analyzed by RT- semi-nested polymerase chain reaction. Based on genomic differences of hepatitis delta antigen coding region of hepatitis delta virus RNA among hepatitis delta virus RNA-positive sera, the polymerase chain reaction products were digested with restriction enzymes and studied by restriction fragment length polymorphism. RESULTS:Out of 35 samples, 13 (38.46%) were positive for hepatitis delta virus RNA by RT- semi-nested polymerase chain reaction. All polymorphisms were shown to be genotype I. Out of 13 hepatitis delta virus RNA-positive (13/35), eight were HBeAg negative. CONCLUSION:Our data indicated that hepatitis delta virus isolates in Tehran are exclusively genotype I.

journal_name

Arch Iran Med

authors

Mirshafiee H,Mahmoodian-Shooshtari M,Sharifi Z,Hosseini SM

subject

Has Abstract

pub_date

2009-05-01 00:00:00

pages

238-43

issue

3

eissn

1029-2977

issn

1735-3947

pii

09123/005

journal_volume

12

pub_type

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