Abstract:
:Paraoxonases (PONs) are a small family of antioxidant enzymes whose antiatherogenic activity is well known. The aim of the present study was the evaluation of the effects of moderate aerobic training on their expression using a rat model. In order to discriminate between PON1 and PON3 enzymatic activity, we took advantage of some differences in their substrate preferences. PON1 and PON3 enzymatic activities and their protein levels were analyzed in plasma and in liver microsomes, and their mRNA levels in the liver. Exercise training did not affect PON1 expression or enzymatic activity but increased PON3 mRNA, protein levels, and enzymatic activity. Training also induced variations in plasma membrane composition, including an increase in polyunsaturated and a decrease in mono- and di-unsaturated fatty acids. On the other hand, acute exercise inhibited PON activities while increasing PON3 protein content in liver microsomes and reversing the relative composition in mono-, di-, and poly-unsaturated fatty acids, suggesting that physical stress, by altering membrane composition, may impair PON release from liver membranes. In conclusion, we documented, for the first time, the presence of PON3 in rat serum and, notably, found that the upregulation of PON3, rather than PON1, appears to be associated with physical training.
journal_name
J Lipid Resjournal_title
Journal of lipid researchauthors
Romani R,De Medio GE,di Tullio S,Lapalombella R,Pirisinu I,Margonato V,Veicsteinas A,Marini M,Rosi Gdoi
10.1194/jlr.M800493-JLR200subject
Has Abstractpub_date
2009-10-01 00:00:00pages
2036-45issue
10eissn
0022-2275issn
1539-7262pii
M800493-JLR200journal_volume
50pub_type
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