Fluorescent leukotriene B4: potential applications.

abstract：:To study the effect of an unstirred layer (UL), we have investigated the exchange efflux kinetics of anandamide at 0 degrees C, pH 7.3, from albumin-free as well as from albumin-filled human red blood cell ghosts to media of various BSA concentrations ([BSA](o)). The rate constant (k(m)) of unidirectional flux from th...

journal_title：Journal of lipid research

authors： Bojesen IN,Hansen HS

更新日期：2006-03-01 00:00:00

abstract：:The central region of apolipoprotein A-I (apoA-I), spanning residues 143--165, has been implicated in lecithin:cholesterol acyltransferase (LCAT) activation and also in high density lipoprotein (HDL) structural rearrangements. To examine the role of individual amino acids in these functions, we constructed, overexpres...

journal_title：Journal of lipid research

authors： Cho KH,Durbin DM,Jonas A

更新日期：2001-03-01 00:00:00

abstract：:The hepatic synthesis of sterol was measured in the male Mongolian gerbil (Meriones unguiculatus) in vivo following the administration of [3H]water by monitoring the incorporation of radioactivity into digitonin-precipitable sterol. A diurnal rhythm in cholesterol synthesis was exhibited under conditions of ad libitum...

journal_title：Journal of lipid research

authors： Mercer NJ,Holub BJ

更新日期：1981-07-01 00:00:00

abstract：:Alcoholic liver disease (ALD) is the most prevalent type of chronic liver disease with significant morbidity and mortality worldwide. ALD begins with simple hepatic steatosis and progresses to alcoholic steatohepatitis, fibrosis, and cirrhosis. The severity of hepatic steatosis is highly associated with the developmen...

journal_title：Journal of lipid research

authors： Jeon S,Carr R

更新日期：2020-04-01 00:00:00

abstract：:We investigated the role of caveolae in uptake and intracellular trafficking of long chain fatty acids (LCFA) in HepG2 human hepatoma cells. The uptake of [(3)H]oleic acid and [(3)H]stearic acid into HepG2 cells was measured by radioactive assays and internalization of the non-metabolizable fluorescent fatty acid 12-(...

journal_title：Journal of lipid research

authors： Pohl J,Ring A,Stremmel W

更新日期：2002-09-01 00:00:00

abstract：:Thermal and lyotropic phase behavior was studied by X-ray diffraction and differential scanning calorimetry for a diether phosphonolipid analog (DEPN-8) of the major lung surfactant glycerophospholipid dipalmitoyl phosphatidylcholine (DPPC). DEPN-8 differs in an ether, rather than an ester, bond at the acyl chain-back...

journal_title：Journal of lipid research

authors： Skita V,Chester DW,Oliver CJ,Turcotte JG,Notter RH

更新日期：1995-05-01 00:00:00

abstract：:Oxidized forms of cholesterol (oxysterols) are present in atherosclerotic lesions and may play an active role in lesion development. For example, 7-ketocholesterol (7KC) inhibits cholesterol efflux from macrophage foam cells induced by apolipoprotein A-I (apoA-I). Such oxysterols may promote foam cell formation in ath...

journal_title：Journal of lipid research

authors： Gelissen IC,Rye KA,Brown AJ,Dean RT,Jessup W

更新日期：1999-09-01 00:00:00

abstract：:Geranylgeranoic acid (GGA) has been reported to induce autophagic cell death via upregulation of lipid-induced unfolded protein response in several human hepatoma-derived cell lines, and its 4,5-didehydro derivative has been developed as a preventive agent against second primary hepatoma in clinical trials. We have pr...

journal_title：Journal of lipid research

authors： Shidoji Y,Tabata Y

更新日期：2019-03-01 00:00:00

abstract：:In the present study, the relative positions of 11 anti-apolipoprotein B monoclonal antibodies have been mapped onto the surface of human low density lipoproteins by electron microscopy. As the epitopes recognized by these antibodies have been previously located on the primary sequence of apoB, these data provide a ma...

journal_title：Journal of lipid research

authors： Chatterton JE,Phillips ML,Curtiss LK,Milne R,Fruchart JC,Schumaker VN

更新日期：1995-09-01 00:00:00

abstract：:In mammals, because they share a single synthetic pathway, n-6/n-3 ratios of dietary PUFAs impact tissue arachidonic acid (ARA) and DHA content. Likewise, SNPs in the human fatty acid desaturase (FADS) gene cluster impact tissue ARA and DHA. Here we tested the feasibility of using heterozygous Fads2-null-mice (HET) as...

journal_title：Journal of lipid research

authors： Su H,Zhou D,Pan YX,Wang X,Nakamura MT

更新日期：2016-11-01 00:00:00

abstract：:The apoE gene exhibits two common polymorphisms that have been associated with both coronary artery disease and Alzheimer's disease. The polymorphisms create the three allelic isoforms E2, E3, and E4 which are encoded by Cys;-Cys, Cys;-Arg, and Arg;-Arg at amino acid positions 112 and 158, respectively. Numerous metho...

journal_title：Journal of lipid research

authors： Bolla MK,Wood N,Humphries SE

更新日期：1999-12-01 00:00:00

abstract：:Previous findings with various murine tumor cell lines suggest an association between ganglioside GM3 and cell cohesive properties. The influence of GM3 on cohesion was studied in two mouse brain tumor cell lines: ependymoblastoma (EPEN) and CT-2A. In culture, the EPEN cells grow as islands and contain GM3 as the only...

journal_title：Journal of lipid research

authors： Bai H,Seyfried TN

更新日期：1997-01-01 00:00:00

abstract：:A sensitive and reproducible method has been developed for separation of the major serum lipoproteins from 1 ml or less of human serum by isopycnic density gradient ultracentrifugation. The serum, applied to a step gradient (total volume 12.8 ml), was spun for 48 hr at 38,000 rpm at 10 degrees C and, in each of the fr...

journal_title：Journal of lipid research

authors： Foreman JR,Karlin JB,Edelstein C,Juhn DJ,Rubenstein AH,Scanu AM

更新日期：1977-11-01 00:00:00

abstract：:Mutational inactivation of NPC1 largely blocks the movement of LDL-derived cholesterol from the lysosome to the metabolically active, cytosolic pool of sterol that is the substrate for steroid hormone production. Such a block might, in theory, lead to deficiencies in circulating levels of testosterone, progesterone, a...

journal_title：Journal of lipid research

authors： Xie C,Richardson JA,Turley SD,Dietschy JM

更新日期：2006-05-01 00:00:00

abstract：:Conditions leading to oxidation of LDL in vivo are still unknown. While the occurrence of oxidized lipoproteins and catalytic free iron in advanced atherosclerotic lesions has been demonstrated, the origin of both is unclear. In vivo, iron metabolism is tightly regulated by iron-binding proteins that ensure that virtu...

journal_title：Journal of lipid research

authors： Balagopalakrishna C,Paka L,Pillarisetti S,Goldberg IJ

更新日期：1999-07-01 00:00:00

abstract：:A method for the preparation of 1-alkyl 2-acyl glycero-3-phosphorylcholine, labeled in the 2-position with oleic acid-1-(14)C is described. This method, based on the acylation of a 1-alkyl glycero-3-phosphorylcholine-CdCl(2) complex which is, in turn, formed from 1-alk-1-enyl glycero-3-phosphorylcholine by hydrogenati...

journal_title：Journal of lipid research

authors： Poulos A

更新日期：1970-09-01 00:00:00

abstract：:Lipoproteins internalized by the LDL receptor (LDLR) are released from this receptor in endosomes through a process that involves acid-dependent conformational changes in the receptor ectodomain. How acidic pH promotes this release process is not well understood. Here, we assessed roles for six histidine residues for ...

journal_title：Journal of lipid research

authors： Dong H,Zhao Z,LeBrun DG,Michaely P

更新日期：2017-02-01 00:00:00

abstract：:Previously, our laboratory demonstrated that ceramide-1-phosphate (C1P) specifically activated group IVA cytosolic phospholipase A(2) (cPLA(2)alpha) in vitro. In this study, we investigated the chain length specificity of this interaction. C1P with an acyl-chain of >or=6 carbons efficiently activated cPLA(2)alpha in v...

journal_title：Journal of lipid research

authors： Wijesinghe DS,Subramanian P,Lamour NF,Gentile LB,Granado MH,Bielawska A,Szulc Z,Gomez-Munoz A,Chalfant CE

更新日期：2009-10-01 00:00:00

abstract：:apoM is a member of the lipocalin superfamily and circulates in plasma attached to HDL particles. apoM plays a role in cholesterol metabolism and has recently been identified as transporter for the signaling lipid, sphingosine-1-phosphate (S1P), in plasma. S1P is implicated in several inflammatory diseases such as mul...

journal_title：Journal of lipid research

authors： Bosteen MH,Dahlbäck B,Nielsen LB,Christoffersen C

更新日期：2015-03-01 00:00:00

abstract：:Sphingolipids comprise a highly diverse and complex class of molecules that serve not only as structural components of membranes but also as signaling molecules. To understand the differential role of sphingolipids in a regulatory network, it is important to use specific and quantitative methods. We developed a novel ...

journal_title：Journal of lipid research

authors： Scherer M,Leuthäuser-Jaschinski K,Ecker J,Schmitz G,Liebisch G

更新日期：2010-07-01 00:00:00

abstract：:H-Rev107 is a protein that was previously cloned as a negative regulator of proto-oncogene Ras and classified as a class II tumor suppressor. Its structural similarity to lecithin retinol acyltransferase and Ca2+-independent phosphatidylethanolamine (PE) N-acyltransferase led us to analyze H-Rev107 as an enzyme involv...

journal_title：Journal of lipid research

authors： Uyama T,Morishita J,Jin XH,Okamoto Y,Tsuboi K,Ueda N

更新日期：2009-04-01 00:00:00

abstract：:We have studied the recognition and uptake of acid lipase by human fibroblasts in order to determine requirements for localization and function of the enzyme in lysosomes. Our approach was based on evidence that a number of acid hydrolases involved in mucopolysaccharide metabolism are secreted from cultured fibroblast...

journal_title：Journal of lipid research

authors： Sando GN,Henke VL

更新日期：1982-01-01 00:00:00

abstract：:Ursodeoxycholic acid (UDCA, ursodiol) is used to prevent damage to the liver in patients with primary biliary cirrhosis. The drug also prevents the progression of colorectal cancer and the recurrence of high-grade colonic dysplasia. However, the molecular mechanism by which UDCA elicits its beneficial effects is not e...

journal_title：Journal of lipid research

authors： Fang C,Filipp FV,Smith JW

更新日期：2012-04-01 00:00:00

abstract：:Liver microsomal lipid peroxidation has been observed in fatal human CCl(4) poisoning, in rats with fatty livers induced by CCl(4) or by yellow phosphorus, and in mice poisoned with 1,1,2,2-tetrachloroethane. These observations suggest the possibility that other instances of toxic liver injury may involve lipid peroxi...

journal_title：Journal of lipid research

authors： Srinivasan S,Recknagel RO

更新日期：1971-11-01 00:00:00

abstract：:Purified liver plasma membranes were prepared from rats fed a high-fat, carbohydrate-free diet or a high-glucose, fat-free diet. Membranes from rats fed the high-fat diet bound significantly less 125I-labeled concanavalin A (Con A) than did those from rats fed the fat-free diets. The magnitude of the binding differenc...

journal_title：Journal of lipid research

authors： Henriquez DS,Tepperman HM,Tepperman J

更新日期：1979-07-01 00:00:00

abstract：:Bacterial lipopolysaccharides (LPSs or endotoxins) can bind most proteins of the lipid transfer/LPS-binding protein (LT/LBP) family in host organisms. The LPS-bound LT/LBP proteins then trigger either an LPS-induced proinflammatory cascade or LPS binding to lipoproteins that are involved in endotoxin inactivation and ...

journal_title：Journal of lipid research

authors： Dusuel A,Deckert V,Pais de Barros JP,van Dongen K,Choubley H,Charron É,Le Guern N,Labbé J,Mandard S,Grober J,Lagrost L,Gautier T

更新日期：2020-12-15 00:00:00

abstract：:Ceramidases catalyze the cleavage of ceramides into sphingosine and fatty acids. Previously, we reported on the use of the RBM14 fluorogenic ceramide analogs to determine acidic ceramidase activity. In this work, we investigated the activity of other amidohydrolases on RBM14 compounds. Both bacterial and human purifie...

journal_title：Journal of lipid research

authors： Casasampere M,Camacho L,Cingolani F,Casas J,Egido-Gabás M,Abad JL,Bedia C,Xu R,Wang K,Canals D,Hannun YA,Mao C,Fabrias G

更新日期：2015-10-01 00:00:00

abstract：:N-acylethanolamines (NAEs) are a group of lipid mediators synthesized in response to a number of physiological and pathological stimuli. Because of the low tissue concentrations of NAEs, analyses often include liquid extraction followed by solid-phase extraction and subsequent quantitation by LC/MS or GC/MS. Reported ...

journal_title：Journal of lipid research

authors： Skonberg C,Artmann A,Cornett C,Hansen SH,Hansen HS

更新日期：2010-10-01 00:00:00

abstract：:The regulation of hepatic and intestinal 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) reductase and acyl-CoA; cholesterol acyltransferase (ACAT) activities by dietary fish oil was examined in the rabbit. Rabbits were fed 10% menhaden oil or menhaden oil plus 1% cholesterol for 14 days. They were compared with animals fed ...

journal_title：Journal of lipid research

authors： Field FJ,Albright EJ,Mathur SN

更新日期：1987-01-01 00:00:00

abstract：:We previously reported that liver-specific overexpression of ABCG5/G8 in mice is not atheroprotective, suggesting that increased biliary cholesterol secretion must be coupled with decreased intestinal cholesterol absorption to increase net sterol loss from the body and reduce atherosclerosis. To evaluate this hypothes...

journal_title：Journal of lipid research

authors： Basso F,Freeman LA,Ko C,Joyce C,Amar MJ,Shamburek RD,Tansey T,Thomas F,Wu J,Paigen B,Remaley AT,Santamarina-Fojo S,Brewer HB Jr

更新日期：2007-01-01 00:00:00