Preparation of labeled alkyl acyl glycerophosphorylcholine.

abstract：:Group IVA cytosolic phospholipase A(2)alpha (cPLA(2)alpha) plays a role in the microbicidal machinery of immune cells by translocating to phagosomes to initiate the production of antimicrobial eicosanoids. In this work, we have studied the involvement of the cationic cluster of cPLA(2)alpha (Lys(488)/Lys(541)/Lys(543)...

journal_title：Journal of lipid research

authors： Casas J,Valdearcos M,Pindado J,Balsinde J,Balboa MA

更新日期：2010-02-01 00:00:00

abstract：:A strategy has been developed for archaebacterial lipid analysis which provides three times the information to describe archaebacterial isolates and is compatible with simultaneous eubacterial/eukaryotic lipid analysis of environmental samples. Eubacterial and micro-eukaryotic biomass, community structure, and nutriti...

journal_title：Journal of lipid research

authors： Hedrick DB,Guckert JB,White DC

更新日期：1991-04-01 00:00:00

abstract：:Using thin-layer chromatography and glass capillary gas-liquid chromatography, we have quantitated the lipids in the germinative, differentiating, and fully cornified layers in human epidermis. As previously noted in nonhuman species, we found progressive depletion of phospholipids coupled with repletion of sterols an...

journal_title：Journal of lipid research

authors： Lampe MA,Williams ML,Elias PM

更新日期：1983-02-01 00:00:00

abstract：:The objective of this study was to establish a new lipoprotein lipase (LPL) and hepatic lipase (HL) activity assay method. Seventy normal volunteers were recruited. Lipase activities were assayed by measuring the increase in absorbance at 546 nm due to the quinoneine dye. Reaction mixture-1 (R-1) contained dioleoylgly...

journal_title：Journal of lipid research

authors： Imamura S,Kobayashi J,Nakajima K,Sakasegawa S,Nohara A,Noguchi T,Kawashiri MA,Inazu A,Deeb SS,Mabuchi H,Brunzell JD

更新日期：2008-07-01 00:00:00

abstract：:Treatment of native human Lp(a) under nondenaturing conditions with dithiothreitol yielded both a lipoprotein particle and a lipid-free protein component that could be separated by either ultracentrifugation at d 1.063 g/ml or heparin-Sepharose chromatography. The protein component only showed antigenicity against ant...

journal_title：Journal of lipid research

authors： Armstrong VW,Walli AK,Seidel D

更新日期：1985-11-01 00:00:00

abstract：:Clostridium absonum was shown to metabolize primary bile acids to give rise to both 7-oxo bile acids and 7 beta-hydroxy (urso) bile acids. At relatively low redox potential (Eh) values, high yields of urso bile acids were achieved (60-75%). If, however, the Eh value of the culture was allowed to rise above approximate...

journal_title：Journal of lipid research

authors： Sutherland JD,Macdonald IA

更新日期：1982-07-01 00:00:00

abstract：:The mitochondrial lipids from avocado fruit, cauliflower buds, and potato tubers, and the lipids of chloroplasts isolated from avocado fruit and from cauliflower leaves were identified and the concentrations were determined. The lipid composition was compared with that of beef heart mitochondria. Phospholipids constit...

journal_title：Journal of lipid research

authors： Schwertner HA,Biale JB

更新日期：1973-03-01 00:00:00

abstract：:Serum amyloid A (SAA) is an acute phase reactant that can become the predominant apolipoprotein of high density lipoprotein (HDL) during severe inflammatory states. However, the function of SAA is unknown. To study the ability of SAA to form HDL in the absence of apolipoprotein A-I, we expressed the mouse SAA pI 6.15 ...

journal_title：Journal of lipid research

authors： Webb NR,de Beer MC,van der Westhuyzen DR,Kindy MS,Banka CL,Tsukamoto K,Rader DL,de Beer FC

更新日期：1997-08-01 00:00:00

abstract：:A family with three heterozygote and two homozygote carriers of the rare apolipoprotein E1 isoform was detected by isoelectric focusing. One of the homozygous patients had type III hyperlipidemia, while the other showed normolipemic dysbetalipoproteinemia. Restriction fragment length analysis as well as allele specifi...

journal_title：Journal of lipid research

authors： Steinmetz A,Assefbarkhi N,Eltze C,Ehlenz K,Funke H,Pies A,Assmann G,Kaffarnik H

更新日期：1990-06-01 00:00:00

abstract：:Polyunsaturated fatty acids, including docosahexaenoic acid (DHA, 22:6n-3), modulate immune responses and exert beneficial immunosuppressive effects, but the molecular mechanisms inhibiting T-cell activation are not yet elucidated. Lipid rafts have been shown to play an important role in the compartmentalization and m...

journal_title：Journal of lipid research

authors： Li Q,Wang M,Tan L,Wang C,Ma J,Li N,Li Y,Xu G,Li J

更新日期：2005-09-01 00:00:00

abstract：:13C-NMR spectroscopy was used to examine the effect of side chain length on the ionization properties and transmembrane transport rate of 3 alpha,7 alpha-dihydroxy bile acids. When solubilized in taurocholate micelles, [23-13C]nor-chenodeoxycholic acid (nor-CDCA) had a pKa of 6.1, similar to that of CDCA (pKa 6.2), it...

journal_title：Journal of lipid research

authors： Ko J,Hamilton JA,Ton-Nu HT,Schteingart CD,Hofmann AF,Small DM

更新日期：1994-05-01 00:00:00

abstract：:Apolipoproteins, such as apolipoprotein A-I (apoA-I), can stimulate cholesterol efflux from cells expressing the ATP binding cassette transporter A1 (ABCA1). The nature of the molecular interaction between these cholesterol acceptors and ABCA1 is controversial, and models suggesting a direct protein-protein interactio...

journal_title：Journal of lipid research

authors： Fitzgerald ML,Morris AL,Chroni A,Mendez AJ,Zannis VI,Freeman MW

更新日期：2004-02-01 00:00:00

abstract：:Extracted tissue phospholipid 31P NMR profiles, obtained from individual porcine lenses subjected to two preservation procedures (acetone desiccation and freeze-drying) and a perchloric acid-extraction procedure, were compared to those from freshly excised lens specimens. Each profile yielded quantitative data on 12 l...

journal_title：Journal of lipid research

authors： Meneses P,Para PF,Glonek T

更新日期：1989-03-01 00:00:00

abstract：:Binding of the medium-chain fatty acids (MCFA), octanoic (OCT) and decanoic (DEC) acid, to human serum albumin (HSA) has been studied by 13C NMR spectroscopy. NMR spectra at 35 degrees C showed an apparently homogeneous binding environment (a single, narrow resonance for the 13C-enriched carboxyl carbon) at different ...

journal_title：Journal of lipid research

authors： Kenyon MA,Hamilton JA

更新日期：1994-03-01 00:00:00

abstract：:The Niemann-Pick C1 (NPC1) protein regulates cholesterol transport from late endosomes-lysosomes to other intracellular compartments. In this article, cholesterol transport to caveolin-1 and caveolin-2 containing compartments, such as the trans-Golgi network (TGN) and plasma membrane caveolae, was examined in normal (...

journal_title：Journal of lipid research

authors： Garver WS,Krishnan K,Gallagos JR,Michikawa M,Francis GA,Heidenreich RA

更新日期：2002-04-01 00:00:00

abstract：:An amino group of phosphatidylethanolamine (PE) is considered as a target for nonenzymatic glycation, and the potential involvement of lipid glycation in the pathogenesis of diabetic complications has generated interest. However, unlike an early glycation product of PE (Amadori-PE), the occurrence and roles of advance...

journal_title：Journal of lipid research

authors： Shoji N,Nakagawa K,Asai A,Fujita I,Hashiura A,Nakajima Y,Oikawa S,Miyazawa T

更新日期：2010-08-01 00:00:00

abstract：:Boiled coffee contains an unidentified lipid that raises serum cholesterol. We studied the effects of the ingestion of coffee oil fractions of increasing purity in volunteers in order to identify the cholesterol-raising factor. In 15 volunteers who ingested 0.75 g/d of a non-triglyceride-fraction from coffee oil for 4...

journal_title：Journal of lipid research

authors： Weusten-Van der Wouw MP,Katan MB,Viani R,Huggett AC,Liardon R,Liardon R,Lund-Larsen PG,Thelle DS,Ahola I,Aro A

更新日期：1994-04-01 00:00:00

abstract：:Human monocyte-derived macrophages secrete lipoprotein lipase (LPL) in culture. The regulation of human macrophage LPL production is poorly understood. Since bacterial lipopolysaccharide (LPS) alters production of several macrophage secretory products, its effect on human monocyte-derived macrophage LPL was tested. LP...

journal_title：Journal of lipid research

authors： White JR,Chait A,Klebanoff SJ,Deeb S,Brunzell JD

更新日期：1988-10-01 00:00:00

abstract：:The activity of lipoprotein lipase isolated from rat postheparin plasma has been determined with synthetic lipids, in the presence and absence of apoprotein of the natural substrate very low density lipoprotein, as a function of medium ion-pair concentration of a number of different inorganic salts. The several kineti...

journal_title：Journal of lipid research

authors： Fielding CJ,Fielding PE

更新日期：1976-05-01 00:00:00

abstract：:Imidazole catalyzed acylations of lysolipids by acyl-CoAs in water at room temperature and at a pH close to neutrality. In the presence of oleoyl-CoA and either lysophosphatidylcholine, 1-palmitoyl-sn-glycero-3-phosphocholine (LPC); lysophosphatidylglycerol, monoacyl-sn-glycero-3-phosphoglycerol; lysophosphatidyl acid...

journal_title：Journal of lipid research

authors： Testet E,Akermoun M,Shimoji M,Cassagne C,Bessoule JJ

更新日期：2002-07-01 00:00:00

abstract：:Human lysosomal acid lipase/cholesteryl ester hydrolase (hLAL) is essential for the intralysosomal metabolism of cholesteryl esters and triglycerides taken up by receptor-mediated endocytosis of lipoprotein particles. The key role of the enzyme in intracellular lipid homeostasis is illustrated by two lysosomal storage...

journal_title：Journal of lipid research

authors： Lohse P,Maas S,Sewell AC,van Diggelen OP,Seidel D

更新日期：1999-02-01 00:00:00

abstract：:Previously, our laboratory demonstrated that ceramide-1-phosphate (C1P) specifically activated group IVA cytosolic phospholipase A(2) (cPLA(2)alpha) in vitro. In this study, we investigated the chain length specificity of this interaction. C1P with an acyl-chain of >or=6 carbons efficiently activated cPLA(2)alpha in v...

journal_title：Journal of lipid research

authors： Wijesinghe DS,Subramanian P,Lamour NF,Gentile LB,Granado MH,Bielawska A,Szulc Z,Gomez-Munoz A,Chalfant CE

更新日期：2009-10-01 00:00:00

abstract：:To determine the extent and microanatomical distribution of extramyocellular adipocytes associated with skeletal muscle, histological, biochemical, nuclear magnetic resonance proton spectroscopic and microcomputed tomography techniques were employed to analyze skeletal muscle samples from lean and obese Sprague-Dawley...

journal_title：Journal of lipid research

authors： Guo Z,Mishra P,Macura S

更新日期：2001-07-01 00:00:00

abstract：:PPARs regulate the expression of genes for energy metabolism in a ligand-dependent manner. PPARs can influence fatty acid oxidation, the level of circulating triglycerides, glucose uptake and insulin sensitivity. Here, we demonstrate that 5-hydroxyeicosapentaenoic acid (HEPE), 8-HEPE, 9-HEPE, 12-HEPE and 18-HEPE (hydr...

journal_title：Journal of lipid research

authors： Yamada H,Oshiro E,Kikuchi S,Hakozaki M,Takahashi H,Kimura K

更新日期：2014-05-01 00:00:00

abstract：:Elongation of very long chain fatty acids (ELOVL)5 is one of seven mammalian fatty acid condensing enzymes involved in microsomal fatty acid elongation. To determine the in vivo substrates and function of ELOVL5, we generated Elovl5(-/-) mice. Studies using liver microsomal protein from wild-type and knockout mice dem...

journal_title：Journal of lipid research

authors： Moon YA,Hammer RE,Horton JD

更新日期：2009-03-01 00:00:00

abstract：:Rabbits were fed either 10% coconut oil, 10% coconut oil and 1% beta-sitosterol, 10% coconut oil and 1% cholesterol, or 10% coconut oil and 1% beta-sitosterol plus 1% cholesterol for 4 weeks. Microsomal membranes from intestines of animals fed the 1% beta-sitosterol diet had 48% less cholesterol and were enriched twof...

journal_title：Journal of lipid research

authors： Field FJ,Mathur SN

更新日期：1983-04-01 00:00:00

abstract：:A novel metabolite of d-delta-tocopherol was isolated from the urine of rats given d-3,4-[3H2]-delta-tocopherol intravenously. The metabolite was collected from the urine of rats given d-delta-tocopherol in the same manner as that of the labeled compound. It was found that the metabolites consisted of sulfate conjugat...

journal_title：Journal of lipid research

authors： Chiku S,Hamamura K,Nakamura T

更新日期：1984-01-01 00:00:00

abstract：:Chronic inflammation contributes to cardiovascular disease. Increased levels of the inflammatory cytokine, TNF-α, are often present in conditions associated with cardiovascular disease risk, and TNF-α induces a number of pro-atherogenic effects in macrovascular endothelial cells, including expression of adhesion molec...

journal_title：Journal of lipid research

authors： Jung HS,Shimizu-Albergine M,Shen X,Kramer F,Shao D,Vivekanandan-Giri A,Pennathur S,Tian R,Kanter JE,Bornfeldt KE

更新日期：2020-01-01 00:00:00

abstract：:Cystic fibrosis (CF) patients and model systems exhibit consistent abnormalities in PUFA metabolism, including increased metabolism of linoleate to arachidonate. Recent studies have connected these abnormalities to increased expression and activity of the Δ6- and Δ5-desaturase enzymes. However, the mechanism connectin...

journal_title：Journal of lipid research

authors： Umunakwe OC,Seegmiller AC

更新日期：2014-07-01 00:00:00

abstract：:The K146N/R147W substitutions in apoE3 were described in patients with a dominant form of type III hyperlipoproteinemia. The effects of these mutations on the in vivo functions of apoE were studied by adenovirus-mediated gene transfer in different mouse models. Expression of the apoE3[K146N/R147W] mutant in apoE-defic...

journal_title：Journal of lipid research

authors： Fotakis P,Vezeridis A,Dafnis I,Chroni A,Kardassis D,Zannis VI

更新日期：2014-07-01 00:00:00