Abstract:
:The objective of this study was to establish a new lipoprotein lipase (LPL) and hepatic lipase (HL) activity assay method. Seventy normal volunteers were recruited. Lipase activities were assayed by measuring the increase in absorbance at 546 nm due to the quinoneine dye. Reaction mixture-1 (R-1) contained dioleoylglycerol solubilized with lauryldimethylaminobetaine, monoacylglycerol-specific lipase, glycerolkinase, glycerol-3-phosphate oxidase, peroxidase, ascorbic acid oxidase, and apolipoprotein C-II (apoC-II). R-2 contained Tris-HCl (pH 8.7) and 4-aminoantipyrine. Automated assay of lipase activities was performed with an automatic clinical analyzer. In the assay for HL + LPL activity, 160 microl R-1 was incubated at 37 degrees C with 2 microl of sample for 5 min, and 80 microl R-2 was added. HL activities were measured under the same conditions without apoC-II. HL and LPL activities were also measured by the conventional isotope method and for HL mass by ELISA. Lipase activity detected in a 1.6 M NaCl-eluted fraction from a heparin-Sepharose column was enhanced by adding purified apoC-II in a dose-dependent manner, whereas that eluted by 0.8 M NaCl was not. Postheparin plasma-LPL and HL activities measured in the present automated method had high correlations with those measured by conventional activity and mass methods. This automated assay method for LPL and HL activities is simple and reliable and can be applied to an automatic clinical analyzer.
journal_name
J Lipid Resjournal_title
Journal of lipid researchauthors
Imamura S,Kobayashi J,Nakajima K,Sakasegawa S,Nohara A,Noguchi T,Kawashiri MA,Inazu A,Deeb SS,Mabuchi H,Brunzell JDdoi
10.1194/jlr.M700528-JLR200subject
Has Abstractpub_date
2008-07-01 00:00:00pages
1431-7issue
7eissn
0022-2275issn
1539-7262pii
M700528-JLR200journal_volume
49pub_type
杂志文章abstract::Some commercial preparations of common natural conjugated bile salts contain impurities (e.g., amines, lipids, and calcium) that are likely to affect their physicochemical properties. A method was developed for purifying commercial preparations of sodium salts of glycine- and taurine-conjugated bile acids. The method ...
journal_title:Journal of lipid research
pub_type: 杂志文章
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更新日期:1995-12-01 00:00:00
abstract::In Tangier disease, absence of ATP binding cassette transporter A1 (ABCA1) results in reduced plasma HDL and elevated triglyceride (TG) levels. We hypothesized that hepatocyte ABCA1 regulates VLDL TG secretion through nascent HDL production. Silencing of ABCA1 expression in oleate-stimulated rat hepatoma cells resulte...
journal_title:Journal of lipid research
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doi:10.1194/jlr.M900083
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journal_title:Journal of lipid research
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journal_title:Journal of lipid research
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更新日期:1994-12-01 00:00:00
abstract::Humans and mice lacking angiopoietin-like protein 3 (ANGPTL3) have pan-hypolipidemia. ANGPTL3 inhibits two intravascular lipases, LPL and endothelial lipase, and the low plasma TG and HDL-cholesterol levels in ANGPTL3 deficiency reflect increased activity of these enzymes. The mechanism responsible for the low LDL-cho...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.M054882
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journal_title:Journal of lipid research
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更新日期:1976-01-01 00:00:00
abstract::MDCO-216, a complex of dimeric recombinant apoA-IMilano (apoA-IM) and palmitoyl-oleoyl-phosphatidylcholine (POPC), was administered to cynomolgus monkeys at 30, 100, and 300 mg/kg every other day for a total of 21 infusions, and effects on lipids, (apo)lipoproteins, and ex-vivo cholesterol efflux capacity were monitor...
journal_title:Journal of lipid research
pub_type: 杂志文章
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更新日期:2013-09-01 00:00:00
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journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.M034579
更新日期:2013-03-01 00:00:00
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更新日期:2006-10-01 00:00:00
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journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1998-09-01 00:00:00
abstract::We analyzed Niemann-Pick type C disease 1 (NPC1) gene in 12 patients with Niemann-Pick type C disease by sequencing both cDNA obtained from fibroblasts and genomic DNA. All the patients were compound heterozygotes. We found 15 mutations, eight of which previously unreported. The comparison of cDNA and genomic DNA reve...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.m200203-jlr200
更新日期:2002-11-01 00:00:00
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journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.D058438
更新日期:2016-01-01 00:00:00
abstract::The lipids of rat milk, the contents of 9-- 10-day-old rat stomach and intestine, lymph, plasma, and liver were quantitated and their fatty acids were analyzed. Rat milk consists of 97% triacylglycerols, of which 35% of the fatty acids are of medium chain length (C8-C12). However, stomach triacylglycerols show a 25% r...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1981-05-01 00:00:00
abstract::Leukotrienes (LTs) play major roles in lung immune responses, and LTD4 is the most potent agonist for cysteinyl LT1, leading to bronchoconstriction and tissue remodeling. Here, we studied LT crosstalk between myeloid cells and pulmonary epithelial cells. Monocytic cells (Mono Mac 6 cell line, primary dendritic cells) ...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.M066910
更新日期:2016-09-01 00:00:00
abstract::Achilles tendon xanthomas are associated with increased cardiovascular risk in patients with familial hypercholesterolemia (FH). Oxidized low density lipoprotein (OxLDL), the antibodies against OxLDL, and the LDL-associated phospholipase A(2) (Lp-PLA(2)) may play important roles in atherogenesis. We investigated the p...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.M600109-JLR200
更新日期:2006-10-01 00:00:00
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journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.M092460
更新日期:2019-09-01 00:00:00
abstract::Recent discovery and characterization of APOAV suggests a role in metabolism of triglyceride (TG)-rich lipoproteins. Previously, variation at the APOAV locus was shown to modestly influence plasma TGs in normolipidemic samples. The aims of this study were to assess the effects of a polymorphism in APOAV (T-1131C) in t...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.M200480-JLR200
更新日期:2003-06-01 00:00:00
abstract::Lipid peroxidation is a common event in health and is greatly accelerated in pro-inflammatory settings such as hypercholesterolemia. Consequently, oxidation-specific epitopes are generated, which are pro-inflammatory and immunogenic, leading to both adaptive and innate responses. Because innate immune mechanisms use c...
journal_title:Journal of lipid research
pub_type: 杂志文章,评审
doi:10.1194/jlr.R800100-JLR200
更新日期:2009-04-01 00:00:00
abstract::Apolipoprotein E plays a critical role in plasma lipoprotein clearance. Peptide models of a highly conserved, N-terminal domain of this protein have been shown to increase the binding of low density lipoprotein (LDL) to fibroblast cell surfaces independently of the low density lipoprotein receptor. Here we provide dat...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1999-04-01 00:00:00
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journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1993-11-01 00:00:00
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journal_title:Journal of lipid research
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journal_title:Journal of lipid research
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doi:10.1194/jlr.M012328
更新日期:2011-02-01 00:00:00
abstract::Ceramide-1-phosphate (C1P) is a novel bioactive sphingolipid formed by the phosphorylation of ceramide catalyzed by ceramide kinase (CERK). In this study, we evaluated the mechanism by which increased C1P during phagocytosis enhances phagocytosis and phagolysosome formation in COS-1 cells expressing hCERK. Stable tran...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.M700442-JLR200
更新日期:2008-03-01 00:00:00
abstract::The uropygial gland secretions produced by female mallards (Anas platyrhynchos) throughout the year were analyzed by thin-layer chromatography and combined gas-liquid chromatography and mass spectrometry. Most of the year, the secretion was composed of wax esters. With the beginning of the mating season in the middle ...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1987-05-01 00:00:00
abstract::The biosynthesis of prostaglandins by isolated rat adrenocortical cells has been studied by determinations of products formed during incubations with labeled arachidonic acid and by radioimmunoassays. Analysis by thin-layer chromatographic separation of silicic acid column fractions indicated that PGE2, PGA2, (B2) and...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1979-01-01 00:00:00
abstract::Thyroid hormone lowers serum cholesterol and alters sterol metabolic processes. This laboratory has previously reported increased biliary lipid secretion as an early effect of triiodothyronine (T3) in the rat. To evaluate whether the bile lipid action of T3 is a primary or secondary effect, the isolated-perfused rat l...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1992-10-01 00:00:00
abstract::Although the synthesis pathways of intracellular triacylglycerol (TAG) species have been well elucidated, assessment of the contribution of an individual pathway to TAG pools in different mammalian organs, particularly under pathophysiological conditions, is difficult, although not impossible. Herein, we developed and...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.M033837
更新日期:2013-04-01 00:00:00
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更新日期:2015-03-01 00:00:00
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pub_type: 杂志文章
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更新日期:2013-11-01 00:00:00
abstract::The sialic acid present in erythrocyte ghosts was estimated by methane chemical ionization mass fragmentography of the trimethylsilyl methyl glycosides. The internal standard was 3,4,6-tris-trimethylsilyl-alpha-phenyl-2-deoxy-2-acetamide-D-glucosaminide, as proposed by Yu and Ledeen (J. Lipid Res. 1970. 11: 506-516). ...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1980-11-01 00:00:00