Abstract:
:Thermal and lyotropic phase behavior was studied by X-ray diffraction and differential scanning calorimetry for a diether phosphonolipid analog (DEPN-8) of the major lung surfactant glycerophospholipid dipalmitoyl phosphatidylcholine (DPPC). DEPN-8 differs in an ether, rather than an ester, bond at the acyl chain-backbone linkage and a headgroup phosphonate (isosteric methylene substitution) versus phosphate constituent. Analysis of lamellar diffraction maxima demonstrated that at high relative humidity (98%) and temperatures below the liquid crystal phase transition (approximately 45 degrees C), DEPN-8 formed interdigitated bilayers with a characteristic periodicity of 41.9-46.5 A. At low humidity the gel phase DEPN-8 bilayers were characteristic of a normal L beta phase with a periodicity equivalent to DPPC (57-59 A). Above the liquid crystal thermal phase transition, bilayer spacing for both DEPN-8 and DPPC was 51-52 A, characteristic of the L alpha phase. Complete assessments of both lamellar and in-plane X-ray scattering used to construct electron density profiles and structure-factor plots for DEPN-8 defined more fully the interdigitated bilayer state at high humidity and low temperature. Compared to DPPC, it is energetically favorable for DEPN-8 to form interdigitated bilayers under conditions of excess water and low temperature. The flexible character of the ether bonds in DEPN-8 allows increased hydrophobic interactions between acyl chains, without generating a steric penalty from the increased packing density of the molecules. Additionally, the ether bond and the phosphonate moiety may allow for more energetically favorable interactions between the choline portion of the headgroup and water. The DEPN-8 ether linkage may also contribute to the improved adsorption and film respreading found previously for this phosphonolipid compared to DPPC.
journal_name
J Lipid Resjournal_title
Journal of lipid researchauthors
Skita V,Chester DW,Oliver CJ,Turcotte JG,Notter RHsubject
Has Abstractpub_date
1995-05-01 00:00:00pages
1116-27issue
5eissn
0022-2275issn
1539-7262journal_volume
36pub_type
杂志文章abstract::We have previously demonstrated the potential usefulness of capillary columns coated with a high temperature polarizable phenylmethylsilicone liquid phase in plasma lipid profiling (Kuksis, A., J.J. Myher, and P. Sandra. 1990. J. Chromatogr. 500: 427-441). The present study reports improved operating conditions along ...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1993-06-01 00:00:00
abstract::Phospholipids serve as central structural components in cellular membranes and as potent mediators in numerous signaling pathways. There are six main classes of naturally occurring phospholipids distinguished by their distinct polar head groups that contain many unique molecular species with distinct fatty acid compos...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.D051581
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abstract::Monospecific antisera were produced to two chylomicron apoproteins (apoB, apoA-I) and utilized for indirect immunofluorescent localization of these apoproteins within rat intestinal epithelium during normal and impaired lipid absorption. Isolated intestinal epithelial cells prepared after different periods of lipid ab...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1978-02-01 00:00:00
abstract::Activation of cultured hepatic stellate cells correlated with an enhanced expression of proteins involved in uptake and storage of fatty acids (FA translocase CD36, Acyl-CoA synthetase 2) and retinol (cellular retinol binding protein type I, CRBP-I; lecithin:retinol acyltransferases, LRAT). The increased expression of...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.M200376-JLR200
更新日期:2003-02-01 00:00:00
abstract::We have developed a procedure to quantify apolipoprotein (apo) B-100, apoB-48, and apoE in human triglyceride-rich lipoproteins. This procedure permits delipidation of small amounts of triglyceride-rich lipoproteins without appreciable losses, and quantification of these apolipoproteins in samples containing as little...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1995-04-01 00:00:00
abstract::Three mammalian cell lines were examined for their ability to metabolize the regulatory oxysterol, 25-hydroxycholesterol, and derepress 3-hydroxy-3-methylglutaryl CoA reductase. In mouse L cell fibroblasts reductase activity was restored with the concomitant metabolism of 25-hydroxycholesterol via side-chain hydroxyla...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1989-06-01 00:00:00
abstract::Hepatocytes and hepatic stellate cells play important roles in retinoid storage and metabolism. Hepatocytes process postprandial retinyl esters and are responsible for secretion of retinol bound to retinol-binding protein (RBP) to maintain plasma retinol levels. Stellate cells are the body's major cellular storage sit...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:2000-06-01 00:00:00
abstract::The methanolyzed lipids of the extreme halophile, Halobacterium cutirubrum, were separated into glycerol diether and glycerol monoether fractions. The diether was shown by synthesis to be 2,3-di-O-(3'R,7'R,11'R,15'-tetramethylhexadecyl)-sn-glycerol. The monoether fraction was separated by thin-layer chromatography on ...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1968-11-01 00:00:00
abstract::Neuroinflammation, caused by 6 days of intracerebroventricular infusion of a low dose of lipopolysaccharide (LPS; 0.5 ng/h), stimulates brain arachidonic acid (AA) metabolism in rats, but 6 weeks of lithium pretreatment reduces this effect. To further understand this action of lithium, we measured concentrations of ei...
journal_title:Journal of lipid research
pub_type: 杂志文章,收录出版
doi:10.1194/jlr.M002469
更新日期:2010-05-01 00:00:00
abstract::Sterol 27-hydroxylase, the mitochondrial enzyme that catalyzes the first step in oxidation of the sterol side chain in hepatic bile acid synthesis, also catalyzes the synthesis of 27-hydroxycholesterol from cholesterol. We have developed a high performance liquid chromatography (HPLC) assay for this enzyme, using eith...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1993-04-01 00:00:00
abstract::The hepatic synthesis of sterol was measured in the male Mongolian gerbil (Meriones unguiculatus) in vivo following the administration of [3H]water by monitoring the incorporation of radioactivity into digitonin-precipitable sterol. A diurnal rhythm in cholesterol synthesis was exhibited under conditions of ad libitum...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1981-07-01 00:00:00
abstract::Glycosylphosphatidylinositols (GPIs) act as membrane anchors of many eukaryotic cell surface proteins. GPIs in various organisms have a common backbone consisting of ethanolamine phosphate (EtNP), three mannoses (Mans), one non-N-acetylated glucosamine, and inositol phospholipid, whose structure is EtNP-6Manα-2Manα-6M...
journal_title:Journal of lipid research
pub_type: 杂志文章,评审
doi:10.1194/jlr.R063313
更新日期:2016-01-01 00:00:00
abstract::This paper presents a case of typical hyperlipoproteinemia type I in a young woman. Her serum triglycerides varied between 2 and 90 mmol/l and she had substantial amounts of apolipoprotein B-48 in fasting plasma. She had no detectable lipoprotein lipase (LPL) activity in post-heparin plasma (less than 0.2 percent of n...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1990-07-01 00:00:00
abstract::Spectrophotometric techniques for determining the activities of lipases, lysophospholipases, and phospholipases are reviewed. These methods involve the use of thioester substrate analogs as well as omega-nitrophenyl derivatives of the corresponding lipids. The most promising results are obtained with the thioester sub...
journal_title:Journal of lipid research
pub_type: 杂志文章,评审
doi:
更新日期:1984-12-15 00:00:00
abstract::We have previously observed that the Ya subunit-containing glutathione (GSH) S-transferases from rat liver exhibit a common high affinity binding site for lithocholic acid, bilirubin, and sulfobromophthalein (BSP) (1984. J. Lipid Res. 25: 1177-1183). Subsequently we found that cholic acid and its amidates bound to a s...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1988-03-01 00:00:00
abstract::The absorption and distribution of deuterated trans- and cis-13-octadecenoic acid (13t-18:1 and 13c-18:1) in plasma lipids were compared to deuterated cis-9-octadecenoic acid (9c-18:1) in two young adult male subjects. A mixture of triglycerides was fed in a multiple-labeled experiment where each triglyceride containe...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1983-01-01 00:00:00
abstract::Autosomal dominant Stargardt-like macular dystrophy (STGD3) in humans results from mutations in elongation of very long chain FAs-like 4 (ELOVL4), which leads to vision loss in young adults. ELOVL4 is an integral endoplasmic reticulum (ER) protein that mediates the elongation of very long chain (VLC) FAs. Mutations in...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.M045443
更新日期:2014-04-01 00:00:00
abstract::Mature endothelial lipase (EL) is a 68 kDa glycoprotein. In HepG2 cells infected with adenovirus encoding human EL, the mature EL was detectable in the cell lysates and heparin-releasable fractions. In contrast, cell media of these cells contained two EL fragments: an N-terminal 40 kDa fragment and a C-terminal 28 kDa...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.M400500-JLR200
更新日期:2005-05-01 00:00:00
abstract::In a previously described method for determining the activity of cerebroside galactosidase, the enzyme preparation was incubated with an emulsion of cerebroside which had been labeled in the galactose moiety. The liberated galactose was separated from the emulsion by liquid-liquid partitioning, but the presence of det...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1971-03-01 00:00:00
abstract::Hypertriglyceridemia induces multiple changes in lipoprotein composition. Here we investigate how one of these modifications, triglyceride (TG) enrichment, affects HDL and LDL function when this alteration occurs under conditions in which more polar components can naturally re-equilibrate. TG-enriched lipoproteins wer...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:2002-08-01 00:00:00
abstract::Serine palmitoyltransferase [EC 2.3.1.50] catalyzes the first unique reaction of sphingolipid biosynthesis. To determine whether or not different rat tissues are capable of initiating this pathway, its activity was determined for microsomes from rat liver, lung, brain, kidney, intestine, spleen, muscle, heart, pancrea...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1985-05-01 00:00:00
abstract::A simple, rapid, and sensitive bioluminescence method for measuring primary bile acids has been developed and validated. The method is based on enzymatic dehydrogenation of bile acids using a bacterial 7 alpha-hydroxysteroid dehydrogenase that is co-immobilized on Sepharose 4B beads with NADH:FMN oxidoreductase and a ...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1982-12-01 00:00:00
abstract::The effect of electron transport chain redox status on activity of the mitochondrial Ca(2+)-independent phospholipase A2 (iPLA2) has been examined. When oxidizing NAD-linked substrates, the enzyme is not active unless deenergization occurs. Uncoupler, rotenone, antimycin A, and cyanide are equally effective at upregul...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.M043307
更新日期:2014-05-01 00:00:00
abstract::Accumulation of foam cells in the neointima represents a key event in atherosclerosis. We previously demonstrated that Tanshinone IIA (Tan), a lipophilic bioactive compound extracted from Salvia miltiorrhiza Bunge, inhibits experimental atherogenesis, yet the detailed mechanisms are not fully understood. In this study...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.M040394
更新日期:2014-02-01 00:00:00
abstract::Apolipoprotein A-IV (apoA-IV) is involved in the metabolism of chylomicrons and high density lipoproteins. It displays genetic polymorphism due to two co-dominant alleles apoA-IV1 and apoA-IV2. The mutation that causes the polymorphism is a G to T substitution in the third base of codon 360 in the apoA-IV2 allele whic...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1991-03-01 00:00:00
abstract::Mouse plasma from strains C57BL/6J and C3H/HeJ includes a high density lipoprotein (HDL) fraction containing apolipoprotein A-I which migrates in the prebeta region upon agarose gel electrophoresis, similar to the prebeta HDL previously reported in humans. This prebeta A-I lipoprotein species has a buoyant density of ...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1990-02-01 00:00:00
abstract::Gap junctions of rat brown adipose tissue (BAT) were studied with the freeze-fracture technique during development. The frequency and the mean area of gap junctions increased after birth, reached a maximal development during the functional phase of the tissue (birth to 3--4 weeks) and decreased while brown adipose tis...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1980-07-01 00:00:00
abstract::The role of plasma free fatty acids (FFA) in the transport of fatty acids from host tissues to Ehrlich ascites carcinoma in mice was studied. [9,10-(3)H] Palmitate complexed to mouse serum (albumin) was injected either intraperitoneally or intravenously into unanesthetized tumor-bearing mice. The incorporation of radi...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1974-07-01 00:00:00
abstract::Sebaceous glands are intriguing glands that are found throughout the human body except on the palms of the hands and soles of the feet. The true function of these glands has yet to be determined, but there are several theories, including antioxidant effects, antibacterial effects, and transport of pheromones. Sebaceou...
journal_title:Journal of lipid research
pub_type: 杂志文章,评审
doi:10.1194/jlr.R700015-JLR200
更新日期:2008-02-01 00:00:00
abstract::Senescence-accelerated mouse-prone (SAMP1; SAMP1@Umz) is an animal model of senile amyloidosis with apolipoprotein A-II (apoA-II) amyloid fibril (AApoAII) deposits. This study was undertaken to investigate the effects of dietary fats on AApoAII deposits in SAMP1 mice when purified diets containing 4% fat as butter, sa...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.M200405-JLR200
更新日期:2003-04-01 00:00:00