Abstract:
:Monospecific antisera were produced to two chylomicron apoproteins (apoB, apoA-I) and utilized for indirect immunofluorescent localization of these apoproteins within rat intestinal epithelium during normal and impaired lipid absorption. Isolated intestinal epithelial cells prepared after different periods of lipid absorption from in situ intestinal segments revealed a rapid increase in fluorescence for both apoproteins that filled the entire apical portion of the cell. Prolonged lipid absorption for as long as 5 hr demonstrated sustained immunofluorescence and gave no indication of a depletion of the intestinal mucosa for either apoprotein during normal lipid absorption. [(3)H]Leucine incorporation into mesenteric lymph chylomicron apoproteins showed a linear decrease in specific activity of total chylomicron protein as well as apoB over 4 hr of a continuous lipid infusion indicating sustained active apoprotein synthesis during prolonged lipid absorption. Acetoxycloheximide, a potent inhibitor of protein synthesis, was employed to determine the dynamics of chylomicron apoproteins during an experimental condition of impaired lipid absorption. In animals with inhibited protein synthesis, fluorescence for both apoproteins was present early in the course of lipid absorption; however, at 60 min after the onset of lipid absorption, fluorescence for both apoproteins was absent. Fluorescence for both apoproteins returned during the recovery of protein synthesis. The present studies have confirmed previous results that localized two chylomicron apoproteins within intestinal epithelial cells. The present studies extend these observations and disclose a rapid and sustained synthesis of these apoproteins during prolonged chylomicron formation. During an experimental condition of impaired protein synthesis there was a marked reduction in the mucosal content of both apoA-I and apoB. These results are the first demonstration of impaired mucosal apoprotein synthesis during an experimental model of impaired lipid absorption.
journal_name
J Lipid Resjournal_title
Journal of lipid researchauthors
Glickman RM,Kilgore A,Khorana Jsubject
Has Abstractpub_date
1978-02-01 00:00:00pages
260-8issue
2eissn
0022-2275issn
1539-7262journal_volume
19pub_type
杂志文章abstract::Biliary secretion of bile acid glucuronides was studied in control rats and in rats with a congenital defect in hepatobiliary transport of organic anions (GY rats). In control animals, hepatobiliary transport of [3H]lithocholic acid 3-O-glucuronide and [3H]cholic acid 3-O-glucuronide was efficient (greater than 95% in...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1989-12-01 00:00:00
abstract::We analyzed Niemann-Pick type C disease 1 (NPC1) gene in 12 patients with Niemann-Pick type C disease by sequencing both cDNA obtained from fibroblasts and genomic DNA. All the patients were compound heterozygotes. We found 15 mutations, eight of which previously unreported. The comparison of cDNA and genomic DNA reve...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.m200203-jlr200
更新日期:2002-11-01 00:00:00
abstract::Small dense LDL (sdLDL) has been reported to be more atherogenic than large buoyant LDL (lbLDL). We examined the metabolism and protein composition of sdLDL and lbLDL in six subjects with combined hyperlipidemia on placebo and rosuvastatin 40 mg/day. ApoB-100 kinetics in triglyceride-rich lipoproteins (TRLs), lbLDL (d...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.M073882
更新日期:2017-07-01 00:00:00
abstract::Cerebrosides containing either threo- or erythro-[3-3-H]sphingosine were synthesized by a new procedure. Glucopyranosyl or galactopyranosyl ceramides were converted to their 3-keto derivatives with 2,3-dichloro-5,6-dicyanobenzoquinone and reduced with 3-H-labeled sodium borohydride. The resulting tritiated cerebroside...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1975-07-01 00:00:00
abstract::In vitro experiments have demonstrated that exogenous phospholipid transfer protein (PLTP), i.e. purified PLTP added to macrophage cultures, influences ABCA1-mediated cholesterol efflux from macrophages to HDL. To investigate whether PLTP produced by the macrophages (i.e., endogenous PLTP) is also part of this process...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.M600051-JLR200
更新日期:2006-08-01 00:00:00
abstract::Fatty liver is frequent in the apolipoprotein B (apoB)-defective genetic form of familial hypobetalipoproteinemia (FHBL), but interindividual variability in liver fat is large. To explain this, we assessed the roles of metabolic factors in 32 affected family members with apoB-defective FHBL and 33 related and unrelate...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.M300508-JLR200
更新日期:2004-05-01 00:00:00
abstract::Apolipoprotein E2 (apoE2)-associated hyperlipidemia is characterized by a disturbed clearance of apoE2-enriched VLDL remnants. Because excess apoE2 inhibits LPL-mediated triglyceride (TG) hydrolysis in vitro, we investigated whether direct or indirect stimulation of LPL activity in vivo reduces the apoE2-associated hy...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.M800009-JLR200
更新日期:2008-05-01 00:00:00
abstract::Peroxisome proliferator-activated receptor alpha (PPAR alpha) is a transcriptional regulator of the expression of mitochondrial thioesterase I (MTE-I) and uncoupling protein 3 (UCP3), which are induced in the heart at the mRNA level in response to diabetes. Little is known about the regulation of protein expression of...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.M600364-JLR200
更新日期:2007-07-01 00:00:00
abstract::Gluco- and galactocerebrosides can be separated by thin-layer chromatography on Silica Gel G prepared with sodium borate solution instead of water. The most successful developing system was chloroform-methanol-water-15 M NH(4)OH 280:70:6:1. ...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1966-05-01 00:00:00
abstract::Geranylgeranoic acid (GGA) has been reported to induce autophagic cell death via upregulation of lipid-induced unfolded protein response in several human hepatoma-derived cell lines, and its 4,5-didehydro derivative has been developed as a preventive agent against second primary hepatoma in clinical trials. We have pr...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.M090548
更新日期:2019-03-01 00:00:00
abstract::Dietary lipids are taken up as FAs by the intestinal epithelium and converted by diacylglycerol acyltransferase (DGAT) enzymes into triglycerides, which are packaged in chylomicrons or stored in cytoplasmic lipid droplets (LDs). DGAT1-deficient patients suffer from vomiting, diarrhea, and protein losing enteropathy, i...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.M094201
更新日期:2019-10-01 00:00:00
abstract::The vitamin D signal transduction system involves a series of cytochrome P450-containing sterol hydroxylases to generate and degrade the active hormone, 1α,25-dihydroxyvitamin D3, which serves as a ligand for the vitamin D receptor-mediated transcriptional gene expression described in companion articles in this review...
journal_title:Journal of lipid research
pub_type: 杂志文章,评审
doi:10.1194/jlr.R031534
更新日期:2014-01-01 00:00:00
abstract::Rhizobium chitolipooligosaccharides (CLOSs) are heterogeneous fatty acylated N-acetyl glucosamine oligomers with variations in both the polar (hydrophilic) oligosaccharide head group and the non-polar (hydrophobic) fatty acyl chain. They trigger root hair deformation and cortical cell divisions in legume roots during ...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1997-06-01 00:00:00
abstract::Proprotein convertase, subtilisin/kexin type 9 (PCSK9), a key regulator of plasma LDL-cholesterol (LDL-c) and cardiovascular risk, is produced in liver and secreted into plasma where it binds hepatic LDL receptors (LDLR), leading to their degradation. PCSK9 is transcriptionally activated by sterol response element-bin...
journal_title:Journal of lipid research
pub_type: 临床试验,杂志文章
doi:10.1194/jlr.P009860
更新日期:2010-11-01 00:00:00
abstract::The inverse relationship between plasma levels of high density lipoprotein (HDL) and coronary heart disease rates has suggested that HDL might influence body stores of cholesterol. Therefore, we have investigated potential relationships between the parameters of body cholesterol metabolism and the plasma levels of HDL...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1985-09-01 00:00:00
abstract::Endothelial dysfunction is a key step in atherosclerosis development. Our recent studies suggested that oxLDL-induced increase in endothelial stiffness plays a major role in dyslipidemia-induced endothelial dysfunction. In this study, we identify oxysterols, as the major component of oxLDL, responsible for the increas...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.M027102
更新日期:2012-07-01 00:00:00
abstract::We have performed plasma lipid profiling using liquid chromatography electrospray ionization tandem mass spectrometry on a population cohort of more than 1,000 individuals. From 10 μl of plasma we were able to acquire comparative measures of 312 lipids across 23 lipid classes and subclasses including sphingolipids, ph...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.P035808
更新日期:2013-10-01 00:00:00
abstract::The infrared (IR) and nuclear magnetic resonance (NMR) spectra of eight Delta(24)-steroids and nine C-24 saturated steroids were examined. NMR spectra allow unambiguous assignment of the biologically important Delta(24)-bond; introduction of a Delta(24)-bond causes the appearance of peaks at Delta 1.60 and 1.68 associ...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1968-01-01 00:00:00
abstract::Electrospray ionization mass spectrometry has previously been used to probe qualitative changes in the phospholipid cardiolipin (CL), but it has rarely been used in a quantitative manner. We assessed changes in the amount of individual molecular species of cardiac CL present in a model of congestive heart failure usin...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.M500031-JLR200
更新日期:2005-06-01 00:00:00
abstract::We have studied the synthesis, modification, and secretion of rat apoE in primary cultures of neonatal aortic smooth muscle cells and adult rat hepatocytes. The cultures were pulsed with [35S]methionine and the intracellular and secreted apoE were immunoprecipitated and analyzed by two-dimensional isoelectric focusing...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1988-07-01 00:00:00
abstract::Increased plasma cholesterol is a known risk factor for cardiovascular disease. Lipoprotein particles transport both cholesterol and triglycerides through the blood. It is thought that the size distribution of these particles codetermines cardiovascular disease risk. New types of measurements can determine the concent...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.M800354-JLR200
更新日期:2009-12-01 00:00:00
abstract::Since the discovery of proprotein convertase subtilisin/kexin type 9 (PCSK9) as an attractive target in the treatment of hypercholesterolemia, multiple anti-PCSK9 therapeutic modalities have been pursued in drug development. The objective of this research is to set the stage for the quantitative benchmarking of two an...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.M092486
更新日期:2019-09-01 00:00:00
abstract::Using a radiometric assay with glycochenodeoxycholic acid as substrate, bile acid:3'-phosphoadenosine-5'-phosphosulfate sulfotransferase activity was found in 105,000 g supernatant fractions of liver, proximal intestine, and adrenal gland homogenates from adult hamsters. Optimum conditions for measurement of the hepat...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1979-11-01 00:00:00
abstract::Smith-Lemli-Opitz syndrome (SLOS) is a genetic disorder characterized by low plasma cholesterol and high 7-dehydrocholesterol (7-DHC). Synthesis of cholesterol and 7-DHC and its metabolites is regulated by HMG-CoA reductase, whose activity can be measured by 24-h excretion of its product mevalonate. We devised a simpl...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.m200163-jlr200
更新日期:2002-10-01 00:00:00
abstract::Severe endothelial abnormalities are a prominent feature in sepsis with cytokines such as tumor necrosis factor (TNF)alpha being implicated in the pathogenesis. As mimic to inflammation, human umbilical vascular endothelial cells (HUVEC) were incubated with TNFalpha for 22 h, in the absence or presence of the omega-6 ...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:2002-06-01 00:00:00
abstract::The two oxysterols, 27-hydroxycholesterol (27OH) and 24S-hydroxycholesterol (24OH), are both inhibitors of cholesterol synthesis and activators of the liver X receptor (LXR) in vitro. Their role as physiological regulators under in vivo conditions is controversial, however. In the present work, we utilized a previousl...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.M034124
更新日期:2013-04-01 00:00:00
abstract::Normal human plasma HDL was applied to a column of heparin-Sepharose in the presence of MnCl(2) and three fractions were obtained by stepwise elution with increasing NaCl concentrations: a non-retained fraction (NR, 78% of protein) and two retained fractions (R(1) and R(2), 18 and 2.5% of protein, respectively). Both ...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1981-11-01 00:00:00
abstract::Inflammation has been proposed to impair HDL function and reverse cholesterol transport (RCT). We investigated the effects of inflammation mediated by zymosan, a yeast glucan, on multiple steps along the RCT pathway in vivo and ex vivo. Acute inflammation with 70 mg/kg zymosan impaired RCT to plasma, liver, and feces ...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.M011122
更新日期:2011-05-01 00:00:00
abstract::Potentially atherogenic triglyceride-rich lipoprotein (TRL) remnants can be isolated and quantitated as remnant-like particles (RLP), using an immunoaffinity gel containing specific anti-human apolipoprotein A-I (apoA-I) and apoB-100 monoclonal antibodies. The aim of the present study was to determine the relationship...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:2000-09-01 00:00:00
abstract::Elaborate control mechanisms of intracellular triacylglycerol (TAG) breakdown are critically involved in the maintenance of energy homeostasis. Hypoxia-inducible lipid droplet-associated protein (HILPDA)/hypoxia-inducible gene-2 (Hig-2) has been shown to affect intracellular TAG levels, yet, the underlying molecular m...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.M082388
更新日期:2018-03-01 00:00:00