Abstract:
:The inverse relationship between plasma levels of high density lipoprotein (HDL) and coronary heart disease rates has suggested that HDL might influence body stores of cholesterol. Therefore, we have investigated potential relationships between the parameters of body cholesterol metabolism and the plasma levels of HDL cholesterol and the major HDL apoproteins. The study involved 55 human subjects who underwent long-term cholesterol turnover studies, as well as plasma lipoprotein and apolipoprotein assays. In order to maximize the likelihood of detecting existing relationships, the subjects were selected to span a wide range of plasma levels of lipids, lipoproteins, and apolipoproteins. Single univariate correlation analyses suggested weak but statistically significant inverse relationships of HDL cholesterol and apoA-I levels with the following model parameters: production rate (PR), the mass of rapidly exchanging body cholesterol (M1), the minimum estimate of the mass of slowly exchanging body cholesterol (M3min), and of the mass of total exchangeable body cholesterol (Mtotmin). These correlations, however, were quantitatively quite small (/r/ = 0.28-0.42) in comparison to the strength of the univariate relationships between body weight and PR (r = 0.76), M1 (r = 0.61), M3min (r = 0.58), and Mtotmin (r = 0.78). Correlations for apoA-II and apoE levels were even smaller than those for apoA-I and HDL cholesterol. In additional analyses using multivariate approaches, HDL cholesterol, apoA-I, apoA-II, and apoE levels were all found not to be independent determinants of the parameters of body cholesterol metabolism (/partial r/ less than 0.17, P greater than 0.3 in all cases). Thus the weak univariate correlations reflect relationships of HDL cholesterol and apoA-I levels with physiological variables, such as body size, which are primarily related to the model parameters. We conclude that plasma levels of HDL cholesterol and apoproteins A-I, A-II, and E are not quantitatively important independent determinants of the mass of slowly exchanging body cholesterol or of other parameters of long-term cholesterol turnover in humans. These studies give no support to the hypothesis that the inverse relationship between HDL cholesterol levels and coronary heart disease rates is mediated via an influence of HDL on body stores of cholesterol.
journal_name
J Lipid Resjournal_title
Journal of lipid researchauthors
Blum CB,Dell RB,Palmer RH,Ramakrishnan R,Seplowitz AH,Goodman DSsubject
Has Abstractpub_date
1985-09-01 00:00:00pages
1079-88issue
9eissn
0022-2275issn
1539-7262journal_volume
26pub_type
杂志文章abstract::In this study, we investigated the hypothesis that regulatory T cells (T(reg)) are involved in the immunomodulatory effects of statins on rheumatoid arthritis (RA) patients. The 12-week study cohort consisted of 55 RA patients and 42 control subjects allocated to either a group treated with atorvastatin (AT) (20 mg/da...
journal_title:Journal of lipid research
pub_type: 杂志文章,随机对照试验
doi:10.1194/jlr.M010876
更新日期:2011-05-01 00:00:00
abstract::A lipid transfer particle (LTP) was isolated and purified from the hemolymph of the adult male American cockroach, Periplaneta americana, essentially according to the method previously developed for the purification of LTP from locust hemolymph. Fast protein liquid chromatography (FPLC) on a Mono Q column was used as ...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1993-04-01 00:00:00
abstract::The concept that hepatic cholesterol synthesis regulates hepatocyte assembly and secretion of apoB-containing lipoproteins remains controversial. The present study was carried out in HepG2 cells to examine the regulation of apoB secretion by the HMG-CoA reductase inhibitor atorvastatin. ApoB accumulation in the media ...
journal_title:Journal of lipid research
pub_type: 杂志文章
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更新日期:1999-06-01 00:00:00
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journal_title:Journal of lipid research
pub_type: 杂志文章,评审
doi:10.1194/jlr.r200012-jlr200
更新日期:2002-12-01 00:00:00
abstract::We measured plasma markers of cholesterol synthesis (lathosterol) and absorption (campesterol, sitosterol, and cholestanol) in order to compare the effects of maximal doses of rosuvastatin with atorvastatin and investigate the basis for the significant individual variation in lipid lowering response to statin therapy....
journal_title:Journal of lipid research
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.1194/jlr.P800042-JLR200
更新日期:2009-04-01 00:00:00
abstract::The concentration of cholesterol carried in low density lipoproteins (LDL-C) is primarily determined by the rate at which LDL-C is produced (Jt) and the rate at which the liver takes up this particle through receptor-dependent transport (Jm). The accumulation of specific dietary fatty acids in the liver profoundly alt...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1994-09-01 00:00:00
abstract::Very few studies have investigated the interrelations between proprotein convertase subtilisin/kexin type 9 (PCSK9) metabolism, cholesterol synthesis, and cholesterol absorption. We aimed to address this issue in a large clinical trial of 245 patients with hypercholesterolemia. Serum lipids, PCSK9, lathosterol (choles...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.P088583
更新日期:2019-01-01 00:00:00
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journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/P900038-JLR200
更新日期:2010-02-01 00:00:00
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journal_title:Journal of lipid research
pub_type: 杂志文章,评审
doi:10.1194/jlr.R026641
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journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.M015081
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journal_title:Journal of lipid research
pub_type: 杂志文章,评审
doi:10.1194/jlr.R119000547
更新日期:2020-04-01 00:00:00
abstract::Acyl-CoA-dependent O-acyltransferases catalyze reactions in which fatty acyl-CoAs are joined to acyl acceptors containing free hydroxyl groups to produce neutral lipids. In this report, we characterize a human multifunctional O-acyltransferase (designated MFAT) that belongs to the acyl-CoA:diacylglycerol acyltransfera...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.M500168-JLR200
更新日期:2005-11-01 00:00:00
abstract::We determined the effect of storage at -70 degrees C on the determination of plasma high density lipoprotein (HDL)-cholesterol. Plasma from 106 subjects was stored for 1, 6, and 12 months, then treated with heparin and MnCl2 to remove other lipoproteins, and HDL-cholesterol was measured. The mean HDL-cholesterol level...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1982-11-01 00:00:00
abstract::The sequences of the plasma apolipoproteins have a high degree of internal homology as they contain several 22-mer internal repeats. These amphipathic helical repeats are considered as the structural and functional units of this class of proteins. We proposed that the 22-mer repeats of the plasma apolipoproteins consi...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1995-08-01 00:00:00
abstract::A method is described for the determination of mevalonate in ultrafiltrates of blood plasma. The method depends on the phosphorylation of mevalonate with [gamma-32P]ATP and mevalonate kinase to 5-[32P]phosphomevalonate, and the subsequent isolation of the 5-[32P]phosphomevalonate together with known amounts of added 5...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1979-08-01 00:00:00
abstract::The activity of phospholipase A on phosphatidyl choline and phosphatidal choline spread as monolayers on phosphate buffers containing snake venom (Crotalus atrox or Naja naja) was studied by measuring the fall of surface potential as a function of time, pH, film pressure, temperature, and concentrations of phosphate a...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1966-03-01 00:00:00
abstract::The HDL Atherosclerosis Treatment Study (HATS) demonstrated a clinical benefit in coronary artery disease patients with low HDL cholesterol (HDL-C) levels treated with simvastatin and niacin (S-N) or S-N plus antioxidants (S-N+A) compared with antioxidants alone or placebo. Angiographically documented stenosis regress...
journal_title:Journal of lipid research
pub_type: 临床试验,杂志文章
doi:10.1194/jlr.M200439-JLR200
更新日期:2003-04-01 00:00:00
abstract::Macrophages contain a neutral cholesteryl ester hydrolase that can be activated by cAMP-dependent protein kinase. Immunological studies strongly suggest that hormone-sensitive lipase (HSL) is probably responsible for the cholesteryl ester hydrolase activity in macrophages; however, due to the very low level of express...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1993-11-01 00:00:00
abstract::Percent cholesterol absorption was measured in 94 normal subjects aged 17- 80 years while consuming diets generally low in cholesterol (mean intake = 226 +/- 126 mg/day). A new dual stable isotope method was used where a cholesterol tracer containing 6 extra mass units was given intravenously and another tracer with 5...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1999-02-01 00:00:00
abstract::The metabolism of chenodeoxycholic acid by Pseudomonas sp. NCIB 10590 under strict anaerobic conditions was studied. A range of unsaturated acidic and neutral metabolites were isolated and identified. The major acidic product was chola-4,6-dien-3-one-24-oic acid whilst the major neutral product was androsta-4,6-dien-3...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1983-09-01 00:00:00
abstract::The effect of 26-hydroxycholesterol and other intermediates in bile acid synthesis on HMG-CoA reductase activity was studied in Chinese hamster ovary (CHO) cell culture. Incubation of CHO cells for 5 hr in 0.25 microM 26-hydroxycholesterol caused a 40% inhibition of HMG-CoA reductase activity. All other intermediates ...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1983-10-01 00:00:00
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journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1983-05-01 00:00:00
abstract::A photocolorimeter for rapid automatic titration of free fatty acids is described. The solvents, absolute ethanol and hexane, form a single-phase titration mixture containing Nile blue indicator. The titrant, NaOH in 60% ethanol, is delivered by a motor-driven microsyringe; as alkali is added and the titration mixture...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1966-11-01 00:00:00
abstract::Lipoprotein lipase has been found to efficiently mediate binding of lipoproteins to cell surfaces and to the low density lipoprotein (LDL) receptor-related protein (LRP) under cell culture conditions (Beisiegel et al. 1991. Proc. Natl. Acad. Sci. USA. 88: 8242-8346). This supports the previously proposed idea that the...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1995-06-01 00:00:00
abstract::A two-step polymerase chain reaction (PCR) method for the rapid detection of the apolipoprotein B(Arg3500----Gln) mutation in a mixture of pooled blood samples is described. In the first step PCR, a short gene fragment surrounding codon 3500 is amplified. Subsequently the reaction product is subjected to a second ampl...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1992-10-01 00:00:00
abstract::Alcohol consumption markedly increases the hepatic output of very low density lipoprotein (VLDL), whereas it decreases the resulting low density lipoprotein (LDL) levels and apolipoprotein B. As ethylation of apoB-lysine renders LDL immunogenic and accelerates their clearance, and as alcoholics develop antibodies agai...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1993-07-01 00:00:00
abstract::This paper describes the purification and some of the properties of an enzyme from human spleen that catalyzes the hydrolysis of sphingomyelin with the formation of ceramide and phosphoryl choline. The enzyme, which is located in the subcellular particulate fraction that sediments between 700 and 8500 g, is readily ma...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1967-05-01 00:00:00
abstract::This report describes the isolation of sarcosylsarcosine conjugate of ursodeoxycholic acid (UDCA) formed during the synthesis of sarcoUDCA by the mixed anhydride method. The compound was characterized by its chemical ionization mass spectrum. The diamino acid conjugate was formed only when the free amino acid was used...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1989-05-01 00:00:00
abstract::This article presents a novel methodology for the analysis of ethanolamine glycerophospholipid (PE) and lysoPE molecular species directly from lipid extracts of biological samples. Through brief treatment of lipid extracts with fluorenylmethoxylcarbonyl (Fmoc) chloride, PE and lysoPE species were selectively derivatiz...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.D500007-JLR200
更新日期:2005-07-01 00:00:00