Abstract:
:A lipid transfer particle (LTP) was isolated and purified from the hemolymph of the adult male American cockroach, Periplaneta americana, essentially according to the method previously developed for the purification of LTP from locust hemolymph. Fast protein liquid chromatography (FPLC) on a Mono Q column was used as the additional step to obtain pure LTP. The electron micrograph of purified cockroach LTP exhibited an unusual and asymmetric shape essentially similar to that reported for Manduca sexta LTP (Ryan et al. 1990. J. Lipid Res. 31:871-879). The cockroach LTP was also found to be basically similar to that of M. sexta and locust in terms of subunit structure and lipid composition, although there were significant differences particularly in the contents of hydrocarbons and diacylglycerol. A simple method for assaying LTP activity was developed, based on the finding that cockroach LTP can catalyze the transfer of labeled hydrocarbons (or diacylglycerol) from labeled high density lipophorin (HDLp) bound with a transfer membrane (Immoblion) to unlabeled HDLp dissolved in saline. This finding reveals that cockroach LTP has the capacity to transfer and/or exchange lipids between lipophorin particles with the same density. It was also demonstrated that cockroach LTP has the capacity to catalyze the transfer and/or exchange of hydrocarbons, in addition to diacylglycerol, between cockroach HDLp and locust low density lipophorin (LDLp).
journal_name
J Lipid Resjournal_title
Journal of lipid researchauthors
Takeuchi N,Chino Hsubject
Has Abstractpub_date
1993-04-01 00:00:00pages
543-51issue
4eissn
0022-2275issn
1539-7262journal_volume
34pub_type
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journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1994-11-01 00:00:00
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journal_title:Journal of lipid research
pub_type: 杂志文章,评审
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journal_title:Journal of lipid research
pub_type: 杂志文章
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journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.M062141
更新日期:2015-11-01 00:00:00
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journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1994-08-01 00:00:00
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journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.M200187-JLR200
更新日期:2003-03-01 00:00:00
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journal_title:Journal of lipid research
pub_type: 杂志文章,评审
doi:10.1194/jlr.R600007-JLR200
更新日期:2006-06-01 00:00:00
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journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:2002-02-01 00:00:00
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journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1985-03-01 00:00:00
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journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1991-10-01 00:00:00
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journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1995-01-01 00:00:00
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journal_title:Journal of lipid research
pub_type: 杂志文章,评审
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更新日期:2020-05-01 00:00:00
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journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.M054056
更新日期:2015-02-01 00:00:00
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journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1985-11-01 00:00:00
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journal_title:Journal of lipid research
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journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.M600431-JLR200
更新日期:2007-03-01 00:00:00
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journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1990-01-01 00:00:00
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pub_type: 杂志文章
doi:10.1016/j.jlr.2021.100024
更新日期:2021-01-13 00:00:00
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journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.M800666-JLR200
更新日期:2009-06-01 00:00:00
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journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
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journal_title:Journal of lipid research
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journal_title:Journal of lipid research
pub_type: 杂志文章,评审
doi:
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journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1992-10-01 00:00:00
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journal_title:Journal of lipid research
pub_type: 杂志文章
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journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.M049809
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journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1993-11-01 00:00:00
abstract::Based on our previous observation that monoclonal antibody anti-apoD-4E11 reacted with several HDL proteins we studied them further with three questions in mind: i) is there common protein polymorphism in healthy individuals? ii) how many proteins are present and what are their characteristics? iii) are they all apoli...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1989-06-01 00:00:00
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journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1995-01-01 00:00:00
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journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:2000-01-01 00:00:00
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journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1993-01-01 00:00:00