Abstract：
:4F is an anti-inflammatory, apolipoprotein A-I (apoA-I)-mimetic peptide that is active in vivo at nanomolar concentrations in the presence of a large molar excess of apoA-I. Physiologic concentrations ( approximately 35 microM) of human apoA-I did not inhibit the production of LDL-induced monocyte chemotactic activity by human aortic endothelial cell cultures, but adding nanomolar concentrations of 4F in the presence of approximately 35 microM apoA-I significantly reduced this inflammatory response. We analyzed lipid binding by surface plasmon resonance. The anti-inflammatory 4F peptide bound oxidized lipids with much higher affinity than did apoA-I. Initially, we examined the binding of PAPC (1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphatidylcholine) and observed that its oxidized products bound 4F with an affinity that was approximately 4-6 orders of magnitude higher than that of apoA-I. This high binding affinity was confirmed in studies with defined lipids and phospholipids. 3F-2 and 3F(14) are also amphipathic alpha-helical octadecapeptides, but 3F-2 inhibits atherosclerosis in mice and 3F(14) does not. Like 4F, 3F-2 also bound oxidized phospholipids with very high affinity, whereas 3F(14) resembled apoA-I. The extraordinary ability of 4F to bind pro-inflammatory oxidized lipids probably accounts for its remarkable anti-inflammatory properties.
journal_name
J Lipid Resjournal_title
Journal of lipid researchauthors
Van Lenten BJ,Wagner AC,Jung CL,Ruchala P,Waring AJ,Lehrer RI,Watson AD,Hama S,Navab M,Anantharamaiah GM,Fogelman AMdoi
10.1194/jlr.M800075-JLR200subject
Has Abstractpub_date
2008-11-01 00:00:00pages
2302-11issue
11eissn
0022-2275issn
1539-7262pii
M800075-JLR200journal_volume
49pub_type
杂志文章abstract：:The HDL Atherosclerosis Treatment Study (HATS) demonstrated a clinical benefit in coronary artery disease patients with low HDL cholesterol (HDL-C) levels treated with simvastatin and niacin (S-N) or S-N plus antioxidants (S-N+A) compared with antioxidants alone or placebo. Angiographically documented stenosis regress...
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更新日期：1990-05-01 00:00:00
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doi：10.1194/jlr.M800364-JLR200
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pub_type： 杂志文章
doi：
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doi：10.1194/jlr.M300417-JLR200
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journal_title：Journal of lipid research
pub_type： 杂志文章
doi：
更新日期：1971-03-01 00:00:00
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journal_title：Journal of lipid research
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更新日期：1998-01-01 00:00:00
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doi：
更新日期：1990-01-01 00:00:00
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doi：10.1194/jlr.M200376-JLR200
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journal_title：Journal of lipid research
pub_type： 杂志文章
doi：
更新日期：1978-01-01 00:00:00
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journal_title：Journal of lipid research
pub_type： 杂志文章
doi：
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pub_type： 杂志文章
doi：10.1194/jlr.M038323
更新日期：2013-11-01 00:00:00
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journal_title：Journal of lipid research
pub_type： 杂志文章
doi：
更新日期：1983-03-01 00:00:00
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journal_title：Journal of lipid research
pub_type： 杂志文章
doi：
更新日期：1988-05-01 00:00:00
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journal_title：Journal of lipid research
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doi：
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doi：
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journal_title：Journal of lipid research
pub_type： 杂志文章
doi：
更新日期：1987-08-01 00:00:00
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doi：10.1194/jlr.M800429-JLR200
更新日期：2009-04-01 00:00:00
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journal_title：Journal of lipid research
pub_type： 杂志文章
doi：
更新日期：1995-03-01 00:00:00
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journal_title：Journal of lipid research
pub_type： 杂志文章
doi：
更新日期：1995-06-01 00:00:00
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journal_title：Journal of lipid research
pub_type： 杂志文章
doi：
更新日期：1986-05-01 00:00:00
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pub_type： 杂志文章,评审
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更新日期：2016-03-01 00:00:00
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journal_title：Journal of lipid research
pub_type： 杂志文章
doi：10.1194/jlr.M500294-JLR200
更新日期：2005-11-01 00:00:00
