Structural analysis of novel bioactive acylated steryl glucosides in pre-germinated brown rice bran.

abstract：:Decreased HDL-cholesterol (HDL-C) and familial combined hyperlipidemia (FCHL) are the two most common familial dyslipidemias predisposing to premature coronary heart disease (CHD). These dyslipidemias share many phenotypic features, suggesting a partially overlapping molecular pathogenesis. This was supported by our p...

journal_title：Journal of lipid research

authors： Lilja HE,Suviolahti E,Soro-Paavonen A,Hiekkalinna T,Day A,Lange K,Sobel E,Taskinen MR,Peltonen L,Perola M,Pajukanta P

更新日期：2004-10-01 00:00:00

abstract：:The quantitative isolation of total glycosphingolipids from crude lipid extracts without contamination from other lipid classes is described. The method consists of (a) acetylation of total lipids with pyridine and acetic anhydride, (b) separation of acetylated glycolipids from nonglycolipids on a magnesia-silica gel ...

journal_title：Journal of lipid research

authors： Saito T,Hakomori SI

更新日期：1971-03-01 00:00:00

abstract：:This report describes the gas-liquid chromatography-mass spectrometry (GLC-MS) of the trimethylsilyl ethers of 5 beta-cholestane-3 alpha, 7 alpha, 12 alpha-triol with mono- or dihydroxy substitution in the side chain. Compounds with 24- and 25-unsaturation in the side chain were also studied. The gas-liquid chromatogr...

journal_title：Journal of lipid research

authors： Tint GS,Dayal B,Batta AK,Shefer S,Cheng FW,Salen G,Mosbach EH

更新日期：1978-11-01 00:00:00

abstract：:The chemical and biochemical properties of cholesterol-enriched and cholesterol-poor chylomicrons from rat lymph have been compared. The enriched particles, prepared from cholesterol-containing lipid dispersions, passed into the duodenum, had four to ten times the cholesteryl ester content of the control chylomicrons ...

journal_title：Journal of lipid research

authors： Fielding CJ,Renston JP,Fielding PE

更新日期：1978-08-01 00:00:00

abstract：:Humans and mice lacking angiopoietin-like protein 3 (ANGPTL3) have pan-hypolipidemia. ANGPTL3 inhibits two intravascular lipases, LPL and endothelial lipase, and the low plasma TG and HDL-cholesterol levels in ANGPTL3 deficiency reflect increased activity of these enzymes. The mechanism responsible for the low LDL-cho...

journal_title：Journal of lipid research

authors： Wang Y,Gusarova V,Banfi S,Gromada J,Cohen JC,Hobbs HH

更新日期：2015-07-01 00:00:00

abstract：:A photocolorimeter for rapid automatic titration of free fatty acids is described. The solvents, absolute ethanol and hexane, form a single-phase titration mixture containing Nile blue indicator. The titrant, NaOH in 60% ethanol, is delivered by a motor-driven microsyringe; as alkali is added and the titration mixture...

journal_title：Journal of lipid research

authors： Noble RP

更新日期：1966-11-01 00:00:00

abstract：:The role of plasma free fatty acids (FFA) in the transport of fatty acids from host tissues to Ehrlich ascites carcinoma in mice was studied. [9,10-(3)H] Palmitate complexed to mouse serum (albumin) was injected either intraperitoneally or intravenously into unanesthetized tumor-bearing mice. The incorporation of radi...

journal_title：Journal of lipid research

authors： Mermier P,Baker N

更新日期：1974-07-01 00:00:00

abstract：:The surface area, A, of contracting fatty acid monolayers was measured as a function of time, t, at constant surface pressure. In the initial temporal phase, ln A was linear with radical t. In a subsequent steady-state phase, ln A was linear with t. The initial desorption coefficient for sodium palmitate, K(i), and th...

journal_title：Journal of lipid research

authors： Patil GS,Matthews RH,Cornwell DG

更新日期：1973-01-01 00:00:00

abstract：:Diet-induced changes in high density lipoprotein (HDL) density and size were studied in patas monkeys. When the animals were switched from a moderate fat-low cholesterol diet to a high fat-high cholesterol (HFHC) diet, the plasma apoA-I levels increased initially in all of the animals. The apoA-I levels remained eleva...

journal_title：Journal of lipid research

authors： Melchior GW,Baker HN,Abee CR,Roheim PS

更新日期：1984-09-01 00:00:00

abstract：:Lipogenesis in vitro from acetate-1-(14)C was studied in human preputial skin and abdominal skin. Radioactive lipids were separated by column chromatography on Florisil and by thin-layer chromatography on silica gel. Radioactivity was incorporated chiefly into the triglyceride, sterol, and polar lipid fractions, while...

journal_title：Journal of lipid research

authors： Vroman HE,Nemecek RA,Hsia SL

更新日期：1969-09-01 00:00:00

abstract：:The objective of this study was to establish a new lipoprotein lipase (LPL) and hepatic lipase (HL) activity assay method. Seventy normal volunteers were recruited. Lipase activities were assayed by measuring the increase in absorbance at 546 nm due to the quinoneine dye. Reaction mixture-1 (R-1) contained dioleoylgly...

journal_title：Journal of lipid research

authors： Imamura S,Kobayashi J,Nakajima K,Sakasegawa S,Nohara A,Noguchi T,Kawashiri MA,Inazu A,Deeb SS,Mabuchi H,Brunzell JD

更新日期：2008-07-01 00:00:00

abstract：:Since spheroidal HDL particles (sHDL) are highly dynamic, molecular dynamics (MD) simulations are useful for obtaining structural models. Here we use MD to simulate sHDL with stoichiometries of reconstituted and circulating particles. The hydrophobic effect during simulations rapidly remodels discoidal HDL containing ...

journal_title：Journal of lipid research

authors： Segrest JP,Jones MK,Catte A

更新日期：2013-10-01 00:00:00

abstract：:Apoliprotein (apo) B-100-containing very low density lipoprotein (VLDL) particles secreted from the liver accumulate in plasma during alimentary lipemia. To determine whether changes of VLDL composition occur in the postprandial state that may render these lipoproteins more atherogenic, apoE, C-I, C-II, and C-III, and...

journal_title：Journal of lipid research

authors： Björkegren J,Hamsten A,Milne RW,Karpe F

更新日期：1997-02-01 00:00:00

abstract：:A two-step polymerase chain reaction (PCR) method for the rapid detection of the apolipoprotein B(Arg3500----Gln) mutation in a mixture of pooled blood samples is described. In the first step PCR, a short gene fragment surrounding codon 3500 is amplified. Subsequently the reaction product is subjected to a second ampl...

journal_title：Journal of lipid research

authors： Ruzicka V,März W,Russ A,Gross W

更新日期：1992-10-01 00:00:00

abstract：:Mice with a targeted mutation of 3beta-hydroxysterol Delta(7)-reductase (Dhcr7) that cannot convert 7-dehydrocholesterol to cholesterol were used to identify the origin of fetal sterols. Because their heterozygous mothers synthesize cholesterol normally, virtually all sterols found in a Dhcr7 knockout fetus having a D...

journal_title：Journal of lipid research

authors： Tint GS,Yu H,Shang Q,Xu G,Patel SB

更新日期：2006-07-01 00:00:00

abstract：:X-linked adrenoleukodystrophy (X-ALD) is a peroxisomal disorder caused by impaired degradation of very long-chain fatty acids (VLCFAs) due to mutations in the ABCD1 gene responsible for VLCFA transport into peroxisomes. Lorenzo's oil, a 4:1 mixture of glyceryl trioleate and glyceryl trierucate, has been used to reduce...

journal_title：Journal of lipid research

authors： Sassa T,Wakashima T,Ohno Y,Kihara A

更新日期：2014-03-01 00:00:00

abstract：:The extent to which the heart can convert alpha-linolenic acid (alpha-LNA, 18:3n-3) to longer chain n-3 PUFAs is not known. Conversion rates can be measured in vivo using radiolabeled alpha-LNA and a kinetic fatty acid model. [1-(14)C]alpha-LNA was infused intravenously for 5 min in unanesthetized rats that had been f...

journal_title：Journal of lipid research

authors： Igarashi M,Ma K,Chang L,Bell JM,Rapoport SI

更新日期：2008-08-01 00:00:00

abstract：:Previous studies have reported both positive and negative effects of culture of islets at high glucose concentrations on regulated insulin secretion. Here, we have reexamined this question in mouse islets and determined the role of changes in lipid synthesis in the effects of glucose. Glucose-stimulated insulin secret...

journal_title：Journal of lipid research

authors： Diraison F,Ravier MA,Richards SK,Smith RM,Shimano H,Rutter GA

更新日期：2008-04-01 00:00:00

abstract：:An enzyme-coupled, colorimetric method for the assay of acyl-CoA synthetase is described. Acyl-CoA formed from fatty acid and CoA by acyl-CoA synthetase was dehydrogenated by acyl-CoA oxidase. Hydrogen peroxide produced was then converted into formaldehyde in the presence of methanol by catalase. The formaldehyde reac...

journal_title：Journal of lipid research

authors： Ichihara K,Shibasaki Y

更新日期：1991-10-01 00:00:00

abstract：:Recent studies suggest that 7-dehydrocholesterol (7-DHC)-derived oxysterols play important roles in the pathophysiology of Smith-Lemli-Opitz syndrome (SLOS), a metabolic disorder that is caused by defective 3β-hydroxysterol-Δ(7)-reductase (DHCR7). Although 14 oxysterols have been identified as the primary products of ...

journal_title：Journal of lipid research

authors： Xu L,Korade Z,Rosado DA Jr,Mirnics K,Porter NA

更新日期：2013-04-01 00:00:00

abstract：:The lipids of rat milk, the contents of 9-- 10-day-old rat stomach and intestine, lymph, plasma, and liver were quantitated and their fatty acids were analyzed. Rat milk consists of 97% triacylglycerols, of which 35% of the fatty acids are of medium chain length (C8-C12). However, stomach triacylglycerols show a 25% r...

journal_title：Journal of lipid research

authors： Fernando-Warnakulasuriya GJ,Staggers JE,Frost SC,Wells MA

更新日期：1981-05-01 00:00:00

abstract：:Rod outer segments (ROSs) are specialized light-sensitive organelles in vertebrate photoreceptor cells. Lipids in ROS are of considerable importance, not only in providing an adequate environment for efficient phototransduction, but also in originating the second messengers involved in signal transduction. ROSs have t...

journal_title：Journal of lipid research

authors： Giusto NM,Pasquaré SJ,Salvador GA,Ilincheta de Boschero MG

更新日期：2010-04-01 00:00:00

abstract：:Familial hypobetalipoproteinemia, a syndrome associated with low plasma cholesterol levels, can be caused by apoB gene mutations. We identified a healthy 42-year-old man whose total plasma cholesterol level was 80 mg/dl. His plasma very low density lipoprotein (VLDL) contained a unique truncated apoB species, apoB-83,...

journal_title：Journal of lipid research

authors： Farese RV Jr,Garg A,Pierotti VR,Vega GL,Young SG

更新日期：1992-04-01 00:00:00

abstract：:More than 100 proteins necessary for eukaryotic cell growth, differentiation, and morphology require posttranslational modification by the covalent attachment of an isoprenoid lipid (prenylation). Prenylated proteins include members of the Ras, Rab, and Rho families, lamins, CENPE and CENPF, and the gamma subunit of m...

journal_title：Journal of lipid research

authors： Lane KT,Beese LS

更新日期：2006-04-01 00:00:00

abstract：:Elongation of very long chain fatty acid-like family member 6 (ELOVL6) is a fatty acyl elongase that performs the initial and rate-limiting condensing reaction required for microsomal elongation of long-chain fatty acids. Our previous in vitro studies suggested that ELOVL6 elongated long-chain saturated fatty acids an...

journal_title：Journal of lipid research

authors： Moon YA,Ochoa CR,Mitsche MA,Hammer RE,Horton JD

更新日期：2014-12-01 00:00:00

abstract：:This study aimed at measuring the influence of a low salt diet on the development of experimental atherosclerosis in moderately hyperlipidemic mice. Experiments were carried out on LDL receptor (LDLR) knockout (KO) mice, or apolipoprotein E (apoE) KO mice on a low sodium chloride diet (LSD) as compared with a normal s...

journal_title：Journal of lipid research

authors： Catanozi S,Rocha JC,Passarelli M,Guzzo ML,Alves C,Furukawa LN,Nunes VS,Nakandakare ER,Heimann JC,Quintão EC

更新日期：2003-04-01 00:00:00

abstract：:We analysed the sensitivity to beta-adrenoceptor agonists in epididymal adipose cells from rats submitted to a stress protocol previously reported to induce alterations in sensitivity to catecholamines in cardiac tissue from rats. Food intake and body weight were lower, whereas adipocytes basal lipolysis was higher (c...

journal_title：Journal of lipid research

authors： Farias-Silva E,Grassi-Kassisse DM,Wolf-Nunes V,Spadari-Bratfisch RC

更新日期：1999-09-01 00:00:00

abstract：:A new genetic variant apolipoprotein (apo), A-I Yame, was discovered during screening for apoA-I genetic variants with isoelectric focusing gel electrophoresis. To investigate the structural abnormality of apoA-I Yame, we amplified the apoA-I gene isolated from the proband's peripheral blood leukocytes with the polyme...

journal_title：Journal of lipid research

authors： Takada Y,Sasaki J,Seki M,Ogata S,Teranishi Y,Arakawa K

更新日期：1991-08-01 00:00:00

abstract：:As microsomal triglyceride transfer protein (MTP) is required for the assembly and secretion of apoB-containing lipoproteins by intestinal epithelial cells, the characterization and regulation of MTP in human intestinal cells, CaCo-2, was studied. CaCo-2 cells express MTP mRNA of 4.2 kb and a 97 kDa subunit of MTP pro...

journal_title：Journal of lipid research

authors： Mathur SN,Born E,Murthy S,Field FJ

更新日期：1997-01-01 00:00:00

abstract：:Ceramidases catalyze the cleavage of ceramides into sphingosine and fatty acids. Previously, we reported on the use of the RBM14 fluorogenic ceramide analogs to determine acidic ceramidase activity. In this work, we investigated the activity of other amidohydrolases on RBM14 compounds. Both bacterial and human purifie...

journal_title：Journal of lipid research

authors： Casasampere M,Camacho L,Cingolani F,Casas J,Egido-Gabás M,Abad JL,Bedia C,Xu R,Wang K,Canals D,Hannun YA,Mao C,Fabrias G

更新日期：2015-10-01 00:00:00