CYP7A1 promoter polymorphism -203A>C affects bile salt synthesis rate in patients after ileal resection.

Abstract:

:Cholesterol 7alpha-hydroxylase (CYP7A1) plays a crucial role in cholesterol metabolism and has been implicated in genetic susceptibility to atherosclerosis. Thus, an understanding of its transcriptional regulation is of considerable importance. We evaluated the effect of a common -203A>C polymorphism in the CYP7A1 promoter region on the activity of CYP7A1, estimated as the ratios of serum 7alpha-hydroxycholest-4-en-3-one (C4) to either total or non-HDL-cholesterol. The study was performed on patients after resection of the distal ileum, leading to upregulation of CYP7A1 activity (n = 65). Healthy volunteers served as the control group (n = 66). Whereas higher CYP7A1 activity was associated with the -203A allele in the patient group (C4/cholesterol ratio, 29.0 vs. 14.8 microg/mmol, P = 0.032; C4/non-HDL-cholesterol ratio, 53.3 vs. 21.3 microg/mmol in -203AA and -203CC, P = 0.017, respectively), no differences were observed in the healthy controls. We conclude that under physiological conditions, the -203A>C polymorphism in the CYP7A1 gene promoter region does not seem to have any clinically relevant effect. However, in patients with severe bile salt malabsorption, this polymorphism markedly affects CYP7A1 activity.

journal_name

J Lipid Res

authors

Lenícek M,Komárek V,Zimolová M,Kovár J,Jirsa M,Lukás M,Vítek L

doi

10.1194/jlr.M800364-JLR200

subject

Has Abstract

pub_date

2008-12-01 00:00:00

pages

2664-7

issue

12

eissn

0022-2275

issn

1539-7262

pii

M800364-JLR200

journal_volume

49

pub_type

杂志文章
  • An LC-MS/MS workflow to characterize 16 regio- and stereoisomeric trihydroxyoctadecenoic acids.

    abstract::Trihydroxyoctadecenoic acids (TriHOMEs) are linoleic acid-derived oxylipins with potential physiological relevance in inflammatory processes as well as in maintaining an intact skin barrier. Due to the high number of possible TriHOME isomers with only subtle differences in their physicochemical properties, the stereoc...

    journal_title:Journal of lipid research

    pub_type: 杂志文章

    doi:10.1194/jlr.D087429

    authors: Fuchs D,Hamberg M,Sköld CM,Wheelock ÅM,Wheelock CE

    更新日期:2018-10-01 00:00:00

  • beta-sitosterol: esterification by intestinal acylcoenzyme A: cholesterol acyltransferase (ACAT) and its effect on cholesterol esterification.

    abstract::Rabbits were fed either 10% coconut oil, 10% coconut oil and 1% beta-sitosterol, 10% coconut oil and 1% cholesterol, or 10% coconut oil and 1% beta-sitosterol plus 1% cholesterol for 4 weeks. Microsomal membranes from intestines of animals fed the 1% beta-sitosterol diet had 48% less cholesterol and were enriched twof...

    journal_title:Journal of lipid research

    pub_type: 杂志文章

    doi:

    authors: Field FJ,Mathur SN

    更新日期:1983-04-01 00:00:00

  • Biochemistry and genetics of inherited disorders of peroxisomal fatty acid metabolism.

    abstract::In humans, peroxisomes harbor a complex set of enzymes acting on various lipophilic carboxylic acids, organized in two basic pathways, alpha-oxidation and beta-oxidation; the latter pathway can also handle omega-oxidized compounds. Some oxidation products are crucial to human health (primary bile acids and polyunsatur...

    journal_title:Journal of lipid research

    pub_type: 杂志文章,评审

    doi:10.1194/jlr.R005959

    authors: Van Veldhoven PP

    更新日期:2010-10-01 00:00:00

  • Non-denaturing polyacrylamide gradient gel electrophoresis for the diagnosis of dysbetalipoproteinemia.

    abstract::Dysbetalipoproteinemia, an uncommon but highly atherogenic mixed hyperlipidemia due to the accumulation of remnants of triglyceride-rich lipoproteins, is characterized by cholesterol-enriched VLDL that migrates in the beta-position on agarose gels. The demonstration of a broad beta-band on agarose gel electrophoresis ...

    journal_title:Journal of lipid research

    pub_type: 杂志文章

    doi:10.1194/jlr.d200013-jlr200

    authors: Blom DJ,Byrnes P,Jones S,Marais AD

    更新日期:2003-01-01 00:00:00

  • Method for recovering sulfolipid from plant lipid extracts.

    abstract::Sulfoquinovosyl diglyceride from plant tissues is recovered in sizeable quantities, free from phospholipids and galactolipids. ...

    journal_title:Journal of lipid research

    pub_type: 杂志文章

    doi:

    authors: Klopfenstein WE,Shigley JW

    更新日期:1966-07-01 00:00:00

  • Interaction between head and neck squamous cell carcinoma cells and fibroblasts in the biosynthesis of PGE2.

    abstract::Prostaglandin (PG)E(2) is relevant in tumor biology, and interactions between tumor and stroma cells dramatically influence tumor progression. We tested the hypothesis that cross-talk between head and neck squamous cell carcinoma (HNSCC) cells and fibroblasts could substantially enhance PGE(2) biosynthesis. We observe...

    journal_title:Journal of lipid research

    pub_type: 杂志文章

    doi:10.1194/jlr.M019695

    authors: Alcolea S,Antón R,Camacho M,Soler M,Alfranca A,Avilés-Jurado FX,Redondo JM,Quer M,León X,Vila L

    更新日期:2012-04-01 00:00:00

  • Chemotherapy selection pressure alters sphingolipid composition and mitochondrial bioenergetics in resistant HL-60 cells.

    abstract::The combination of daunorubicin (dnr) and cytarabine (Ara-C) is a cornerstone of treatment for acute myelogenous leukemia (AML); resistance to these drugs is a major cause of treatment failure. Ceramide, a sphingolipid (SL), plays a critical role in cancer cell apoptosis in response to chemotherapy. Here, we investiga...

    journal_title:Journal of lipid research

    pub_type: 杂志文章

    doi:10.1194/jlr.RA119000251

    authors: Kao LP,Morad SAF,Davis TS,MacDougall MR,Kassai M,Abdelmageed N,Fox TE,Kester M,Loughran TP Jr,Abad JL,Fabrias G,Tan SF,Feith DJ,Claxton DF,Spiegel S,Fisher-Wellman KH,Cabot MC

    更新日期:2019-09-01 00:00:00

  • Statin action enriches HDL3 in polyunsaturated phospholipids and plasmalogens and reduces LDL-derived phospholipid hydroperoxides in atherogenic mixed dyslipidemia.

    abstract::Atherogenic mixed dyslipidemia associates with oxidative stress and defective HDL antioxidative function in metabolic syndrome (MetS). The impact of statin treatment on the capacity of HDL to inactivate LDL-derived, redox-active phospholipid hydroperoxides (PCOOHs) in MetS is indeterminate. Insulin-resistant, hypertri...

    journal_title:Journal of lipid research

    pub_type: 临床试验,杂志文章

    doi:10.1194/jlr.P068585

    authors: Orsoni A,Thérond P,Tan R,Giral P,Robillard P,Kontush A,Meikle PJ,Chapman MJ

    更新日期:2016-11-01 00:00:00

  • Gas--liquid chromatography--mass spectrophotometry of hydroxylated octadecanols derived from hydroxylated stearic acids.

    abstract::A gas-liquid chromatographic-mass spectrometric method of determining the position of oxygen atoms on polyfunctional fatty acids has been explored. The method consists of reduction of keto, hydroperoxy, epoxy, and carboxylic acid groups with LiAlH(4) to the corresponding alcohols; trimethylsilylation with bis(trimethy...

    journal_title:Journal of lipid research

    pub_type: 杂志文章

    doi:

    authors: Esselman WJ,Clagett CO

    更新日期:1969-03-01 00:00:00

  • The quantitative assay of 3-hydroxy-3-methylglutaryl coenzyme A reductase: comparison of a thin-layer chromatographic assay with a rapid chloroform extraction assay.

    abstract::Two assays for the quantitative measurement of HMG-CoA reductase, the major regulatory enzyme in hepatic cholesterol biosynthesis, are described. The first of these procedures employs thin-layer chromatography (TLC) of the reaction product on plastic-backed silica gel G strips impregnated with ammonium carbonate. The ...

    journal_title:Journal of lipid research

    pub_type: 杂志文章

    doi:

    authors: Ackerman ME,Redd WL,Tormanen CD,Hardgrave JE,Scallen TJ

    更新日期:1977-05-01 00:00:00

  • A new molecular defect in the lecithin: cholesterol acyltransferase (LCAT) gene associated with fish eye disease.

    abstract::We report a new genetic defect in the lecithin:cholesterol acyltransferase (LCAT) gene associated with classical clinical and biochemical features of fish eye disease. The 63-year-old Australian female proband also suffers from non-insulin-dependent (type II) diabetes mellitus. She presented with corneal opacities, ma...

    journal_title:Journal of lipid research

    pub_type: 杂志文章

    doi:

    authors: Contacos C,Sullivan DR,Rye KA,Funke H,Assmann G

    更新日期:1996-01-01 00:00:00

  • Determination of mRNA levels of cholesterol biosynthesis enzymes and LDL receptor using ribonuclease protection assay.

    abstract::We designed a rapid method for determining mRNA content of cholesterol biosynthesis enzymes and LDL receptor (LDLR) using a ribonuclease protection assay (RPA). 32P-labeled cRNA fragments for genes of human LDLR and the enzymes HMG-CoA synthase (HMGS), HMG-CoA reductase (HMGR), mevalonate kinase (MK), farnesyl pyropho...

    journal_title:Journal of lipid research

    pub_type: 杂志文章

    doi:

    authors: Shimokawa T,Kawabe Y,Honda M,Yazaki Y,Matsumoto A,Itakura H,Kodama T

    更新日期:1995-09-01 00:00:00

  • ESI-MS quantitation of increased sphingomyelin in Niemann-Pick disease type B HDL.

    abstract::HDLs have been proposed to have antiatherogenic properties because of their role in reverse cholesterol transport as lipid acceptors. To elucidate the phospholipid profile of these particles, we used electrospray ionization mass spectrometry to examine the phosphatidylcholine (PC) and sphingomyelin (SM) composition of...

    journal_title:Journal of lipid research

    pub_type: 杂志文章

    doi:10.1194/jlr.M500011-JLR200

    authors: Lee CY,Lesimple A,Larsen A,Mamer O,Genest J

    更新日期:2005-06-01 00:00:00

  • Loss of lipid to plastic tubing.

    abstract::14C-labeled oleic acid and (3)H-labeled monoether in a bile salt solution were perfused through three types of plastic tubing. Large proportions of lipid were lost to the walls of silicone rubber and polyvinyl chloride tubes. The major portion of the lipid lost was recoverable only when chloroform-methanol was perfuse...

    journal_title:Journal of lipid research

    pub_type: 杂志文章

    doi:

    authors: Lee KY

    更新日期:1971-09-01 00:00:00

  • Apolipoprotein E phenotypes in Finnish youths: a cross-sectional and 6-year follow-up study.

    abstract::Apolipoprotein E (apoE) polymorphism is a genetic determinant of plasma lipid levels and of coronary heart disease (CHD) risk. We determined the apoE phenotypes and plasma lipid levels in 1577 youths aged 3 to 18 years in 1980. The subjects were randomly selected from five areas of Finland. ApoE phenotyping was perfor...

    journal_title:Journal of lipid research

    pub_type: 杂志文章

    doi:

    authors: Lehtimäki T,Moilanen T,Viikari J,Akerblom HK,Ehnholm C,Rönnemaa T,Marniemi J,Dahlen G,Nikkari T

    更新日期:1990-03-01 00:00:00

  • Phytanoyl-CoA hydroxylase from rat liver. Protein purification and cDNA cloning with implications for the subcellular localization of phytanic acid alpha-oxidation.

    abstract::Phytanoyl-CoA hydroxylase (PhyH) catalyzes the conversion of phytanoyl-CoA to 2-hydroxyphytanoyl-CoA, which is the first step in the phytanic acid alpha-oxidation pathway. Recently, several studies have shown that in humans, phytanic acid alpha-oxidation is localized in peroxisomes. In rat, however, the alpha-oxidatio...

    journal_title:Journal of lipid research

    pub_type: 杂志文章

    doi:

    authors: Jansen GA,Ofman R,Denis S,Ferdinandusse S,Hogenhout EM,Jakobs C,Wanders RJ

    更新日期:1999-12-01 00:00:00

  • ApoE2-associated hypertriglyceridemia is ameliorated by increased levels of apoA-V but unaffected by apoC-III deficiency.

    abstract::Apolipoprotein E2 (apoE2)-associated hyperlipidemia is characterized by a disturbed clearance of apoE2-enriched VLDL remnants. Because excess apoE2 inhibits LPL-mediated triglyceride (TG) hydrolysis in vitro, we investigated whether direct or indirect stimulation of LPL activity in vivo reduces the apoE2-associated hy...

    journal_title:Journal of lipid research

    pub_type: 杂志文章

    doi:10.1194/jlr.M800009-JLR200

    authors: Gerritsen G,van der Hoogt CC,Schaap FG,Voshol PJ,Kypreos KE,Maeda N,Groen AK,Havekes LM,Rensen PC,van Dijk KW

    更新日期:2008-05-01 00:00:00

  • Inhibition of lysosomal protein degradation inhibits the basal degradation of 3-hydroxy-3-methylglutaryl coenzyme A reductase.

    abstract::The effect of inhibiting lysosomal protein degradation on the activity of 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase was determined using a mouse mammary cell line (TS-85) which expresses a temperature-sensitive mutation in the ubiquitin degradative pathway. Incubating cells for 18 hr in medium containi...

    journal_title:Journal of lipid research

    pub_type: 杂志文章

    doi:

    authors: Tanaka RD,Li AC,Fogelman AM,Edwards PA

    更新日期:1986-03-01 00:00:00

  • Pharmacological properties of a novel ACAT inhibitor (CP-113,818) in cholesterol-fed rats, hamsters, rabbits, and monkeys.

    abstract::The novel acyl-CoA:cholesterol acyltransferase (ACAT) inhibitor CP-113,818 has been characterized in vitro against ACAT isolated from liver and intestine from a variety of species including human subjects, and in vivo in cholesterol-fed rats, hamsters, rabbits, and two species of nonhuman primates. CP-113,818 is a pot...

    journal_title:Journal of lipid research

    pub_type: 杂志文章

    doi:

    authors: Marzetta CA,Savoy YE,Freeman AM,Long CA,Pettini JL,Hagar RE,Inskeep PB,Davis K,Stucchi AF,Nicolosi RJ

    更新日期:1994-10-01 00:00:00

  • Hepatic apolipoprotein E expression promotes very low density lipoprotein-apolipoprotein B production in vivo in mice.

    abstract::In addition to its role in the uptake of apolipoprotein B (apoB)-containing lipoproteins, apoE promotes hepatic very low density lipoprotein-triglyceride (VLDL-TG) production in animal models. However, it is not known if apoE increases the amount of TG per VLDL particle or the number of VLDL particles secreted. VLDL-a...

    journal_title:Journal of lipid research

    pub_type: 杂志文章

    doi:

    authors: Maugeais C,Tietge UJ,Tsukamoto K,Glick JM,Rader DJ

    更新日期:2000-10-01 00:00:00

  • An in situ perfusion protocol of rat epididymal adipose tissue useful in metabolic studies.

    abstract::Experimental approaches involving the perfusion of tissues and organs offer the advantage of improved physiological relevance over the use of isolated tissues or cells while at the same time being much more controlled and tissue-specific than studies in vivo. Nevertheless, there have been few metabolic studies perform...

    journal_title:Journal of lipid research

    pub_type: 杂志文章

    doi:10.1194/jlr.D500016-JLR200

    authors: Cònsol G,Moles A,Ricart-Jané D,Llobera M

    更新日期:2005-08-01 00:00:00

  • Interconversion of prebeta-migrating lipoproteins containing apolipoprotein A-I and HDL.

    abstract::Mouse plasma from strains C57BL/6J and C3H/HeJ includes a high density lipoprotein (HDL) fraction containing apolipoprotein A-I which migrates in the prebeta region upon agarose gel electrophoresis, similar to the prebeta HDL previously reported in humans. This prebeta A-I lipoprotein species has a buoyant density of ...

    journal_title:Journal of lipid research

    pub_type: 杂志文章

    doi:

    authors: Ishida BY,Albee D,Paigen B

    更新日期:1990-02-01 00:00:00

  • Effects on plasma lipoproteins of monounsaturated, saturated, and polyunsaturated fatty acids in the diet of African green monkeys.

    abstract::Work by other investigators has shown that an increase in dietary content of monounsaturated fatty acids can result in a decreased plasma low density lipoprotein (LDL) cholesterol concentration. This observation, combined with the epidemiologic evidence that monounsaturated fat-rich diets are associated with decreased...

    journal_title:Journal of lipid research

    pub_type: 杂志文章

    doi:

    authors: Rudel LL,Haines JL,Sawyer JK

    更新日期:1990-10-01 00:00:00

  • Locus for quantitative HDL-cholesterol on chromosome 10q in Finnish families with dyslipidemia.

    abstract::Decreased HDL-cholesterol (HDL-C) and familial combined hyperlipidemia (FCHL) are the two most common familial dyslipidemias predisposing to premature coronary heart disease (CHD). These dyslipidemias share many phenotypic features, suggesting a partially overlapping molecular pathogenesis. This was supported by our p...

    journal_title:Journal of lipid research

    pub_type: 杂志文章

    doi:10.1194/jlr.M400141-JLR200

    authors: Lilja HE,Suviolahti E,Soro-Paavonen A,Hiekkalinna T,Day A,Lange K,Sobel E,Taskinen MR,Peltonen L,Perola M,Pajukanta P

    更新日期:2004-10-01 00:00:00

  • A rapid method to assess the hydrophobicity of the intestinal microvillus membrane in vivo.

    abstract::Absorption rates for many biologically important compounds are determined by the relative hydrophobicity of the jejunal microvillus membrane. An estimate of this parameter may be obtained by measuring the incremental change in free energy that occurs when a methylene group partitions into the bilayer form an external ...

    journal_title:Journal of lipid research

    pub_type: 杂志文章

    doi:

    authors: Meddings JB,Dietschy JM

    更新日期:1989-08-01 00:00:00

  • Identification of domains in apoA-I susceptible to proteolysis by mast cell chymase. Implications for HDL function.

    abstract::When stimulated, rat serosal mast cells degranulate and secrete a cytoplasmic neutral protease, chymase. We studied the fragmentation of apolipoprotein (apo) A-I during proteolysis of HDL(3) by chymase, and examined how chymase-dependent proteolysis interfered with the binding of eight murine monoclonal antibodies (Ma...

    journal_title:Journal of lipid research

    pub_type: 杂志文章

    doi:

    authors: Lee M,Uboldi P,Giudice D,Catapano AL,Kovanen PT

    更新日期:2000-06-01 00:00:00

  • Appraisal of hepatic lipase and lipoprotein lipase activities in mice.

    abstract::A variety of methods are currently used to analyze HL and LPL activities in mice. In search of a simple methodology, we analyzed mouse preheparin and postheparin plasma LPL and HL activities using specific polyclonal antibodies raised in rabbit against rat HL (anti-HL) and in goat against rat LPL (anti-LPL). As an alt...

    journal_title:Journal of lipid research

    pub_type: 杂志文章

    doi:10.1194/jlr.D700021-JLR200

    authors: Dallinga-Thie GM,Zonneveld-de Boer AJ,van Vark-van der Zee LC,van Haperen R,van Gent T,Jansen H,De Crom R,van Tol A

    更新日期:2007-12-01 00:00:00

  • Further validation of the plasma isotope ratio method for measurement of cholesterol absorption in man.

    abstract::Recently we evaluated an isotope ratio method (IRM) for measurement of cholesterol absorption in 14 patients (15 experiments) hospitalized in the metabolic ward by comparing it to simultaneous measurements with a fecal radioactivity method (FRM) and found good to excellent agreement between two procedures (Samuel, P.,...

    journal_title:Journal of lipid research

    pub_type: 杂志文章

    doi:

    authors: Samuel P,McNamara DJ,Ahrens EH Jr,Crouse JR,Parker T

    更新日期:1982-03-01 00:00:00

  • Activities of serine palmitoyltransferase (3-ketosphinganine synthase) in microsomes from different rat tissues.

    abstract::Serine palmitoyltransferase [EC 2.3.1.50] catalyzes the first unique reaction of sphingolipid biosynthesis. To determine whether or not different rat tissues are capable of initiating this pathway, its activity was determined for microsomes from rat liver, lung, brain, kidney, intestine, spleen, muscle, heart, pancrea...

    journal_title:Journal of lipid research

    pub_type: 杂志文章

    doi:

    authors: Merrill AH Jr,Nixon DW,Williams RD

    更新日期:1985-05-01 00:00:00

  • Hormonal regulation of the differentiation of rat adipocyte precursor cells in primary culture.

    abstract::A reproducible cell culture system is described that allows the study of adipose conversion in fibroblast-like cells isolated by collagenase digestion of epididymal and perirenal adipose tissue from male rats weighing 70-200 g. Adipose conversion as measured by lipid accumulation and increase in glycerophosphate dehyd...

    journal_title:Journal of lipid research

    pub_type: 杂志文章

    doi:

    authors: Wiederer O,Löffler G

    更新日期:1987-06-01 00:00:00