Abstract:
:The hypothesis of lipid domains in cellular plasma membranes is well established. However, direct visualization of the domains has been difficult. Here we report direct visualization of plasma membrane cholesterol microdomains modulated by agents that affect cholesterol trafficking to and from the plasma membrane. The cholesterol microdomains were visualized with a monoclonal antibody that specifically detects ordered cholesterol arrays. These unique cholesterol microdomains were induced on macrophages and fibroblasts when they were enriched with cholesterol in the presence of an ACAT inhibitor, to block esterification of excess cellular cholesterol. Induction of the plasma membrane cholesterol microdomains could be blocked by agents that inhibit trafficking of cholesterol to the plasma membrane and by cholesterol acceptors that remove cholesterol from the plasma membrane. In addition, plasma membrane cholesterol microdomains did not develop in mutant Niemann-Pick type C fibroblasts, consistent with the defect in cholesterol trafficking reported for these cells. The induction of plasma membrane cholesterol microdomains on inhibition of ACAT helps explain how ACAT inhibition promotes cholesterol efflux from cells in the presence of cholesterol acceptors such as HDL. The anti-cholesterol monoclonal antibody also detected extracellular cholesterol-containing particles that accumulated most prominently during cholesterol enrichment of less differentiated human monocyte-macrophages. For the first time, cholesterol microdomains have been visualized that function in cholesterol trafficking to and from the plasma membrane.
journal_name
J Lipid Resjournal_title
Journal of lipid researchauthors
Kruth HS,Ifrim I,Chang J,Addadi L,Perl-Treves D,Zhang WYkeywords:
subject
Has Abstractpub_date
2001-09-01 00:00:00pages
1492-500issue
9eissn
0022-2275issn
1539-7262journal_volume
42pub_type
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journal_title:Journal of lipid research
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journal_title:Journal of lipid research
pub_type: 杂志文章
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更新日期:1978-03-01 00:00:00
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journal_title:Journal of lipid research
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pub_type: 杂志文章
doi:
更新日期:1993-11-01 00:00:00
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journal_title:Journal of lipid research
pub_type: 杂志文章
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更新日期:1967-09-01 00:00:00
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journal_title:Journal of lipid research
pub_type: 杂志文章
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更新日期:1982-11-01 00:00:00
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journal_title:Journal of lipid research
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