A novel semiautomated method for the estimation of free fatty acid in serum or plasma.

Abstract:

:A modification of the semiautomated assay method of Antonis (1965. J. Lipid Res. 6:307-312) for free fatty acid is presented. Free fatty acids are extracted from serum or plasma into di-n-butyl ether-2-methoxyethanol; the extract is almost free from phospholipids. The acids are analyzed in a portion of extract by a copper soap method using diphenylcarbazide. The extractant, being less dense than water, is easily separated from an aqueous phase both in the extraction of samples and in the assay of copper soaps. The assay is comparable in accuracy with well-tried titrimetric methods and is quicker and easier to operate.

journal_name

J Lipid Res

authors

Bowyer DE,Cridland JS,King JP

subject

Has Abstract

pub_date

1978-02-01 00:00:00

pages

274-80

issue

2

eissn

0022-2275

issn

1539-7262

journal_volume

19

pub_type

杂志文章
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    authors: Burgess JW,Marcel YL

    更新日期:2001-09-01 00:00:00

  • Immunogenicity of homologous low density lipoprotein after methylation, ethylation, acetylation, or carbamylation: generation of antibodies specific for derivatized lysine.

    abstract::We previously showed that immunization of guinea pigs with reductively glucosylated guinea pig low density lipoprotein (LDL) or albumin resulted in the formation of antibodies specific for the glucosylated protein. The present studies were done to determine if modifications of homologous LDL or albumin, other than add...

    journal_title:Journal of lipid research

    pub_type: 杂志文章

    doi:

    authors: Steinbrecher UP,Fisher M,Witztum JL,Curtiss LK

    更新日期:1984-10-01 00:00:00

  • Crystal structures of complexes of vitamin D receptor ligand-binding domain with lithocholic acid derivatives.

    abstract::The secondary bile acid lithocholic acid (LCA) and its derivatives act as selective modulators of the vitamin D receptor (VDR), although their structures fundamentally differ from that of the natural hormone 1α,25-dihydroxyvitamin D3 [1,25(OH)2D3)]. Here, we have determined the crystal structures of the ligand-binding...

    journal_title:Journal of lipid research

    pub_type: 杂志文章

    doi:10.1194/jlr.M038307

    authors: Masuno H,Ikura T,Morizono D,Orita I,Yamada S,Shimizu M,Ito N

    更新日期:2013-08-01 00:00:00

  • Rare DEGS1 variant significantly alters de novo ceramide synthesis pathway.

    abstract::The de novo ceramide synthesis pathway is essential to human biology and health, but genetic influences remain unexplored. The core function of this pathway is the generation of biologically active ceramide from its precursor, dihydroceramide. Dihydroceramides have diverse, often protective, biological roles; converse...

    journal_title:Journal of lipid research

    pub_type: 杂志文章

    doi:10.1194/jlr.P094433

    authors: Blackburn NB,Michael LF,Meikle PJ,Peralta JM,Mosior M,McAhren S,Bui HH,Bellinger MA,Giles C,Kumar S,Leandro AC,Almeida M,Weir JM,Mahaney MC,Dyer TD,Almasy L,VandeBerg JL,Williams-Blangero S,Glahn DC,Duggirala R,Ko

    更新日期:2019-09-01 00:00:00

  • Stereochemistry of the peroxisomal branched-chain fatty acid alpha- and beta-oxidation systems in patients suffering from different peroxisomal disorders.

    abstract::Phytanic acid (3,7,11,15-tetramethylhexadecanoic acid) is a branched-chain fatty acid derived from dietary sources and broken down in the peroxisome to pristanic acid (2,6,10,14-tetramethylpentadecanoic acid) via alpha-oxidation. Pristanic acid then undergoes beta-oxidation in peroxisomes. Phytanic acid naturally occu...

    journal_title:Journal of lipid research

    pub_type: 杂志文章

    doi:

    authors: Ferdinandusse S,Rusch H,van Lint AE,Dacremont G,Wanders RJ,Vreken P

    更新日期:2002-03-01 00:00:00

  • Effects of side chain length on ionization behavior and transbilayer transport of unconjugated dihydroxy bile acids: a comparison of nor-chenodeoxycholic acid and chenodeoxycholic acid.

    abstract::13C-NMR spectroscopy was used to examine the effect of side chain length on the ionization properties and transmembrane transport rate of 3 alpha,7 alpha-dihydroxy bile acids. When solubilized in taurocholate micelles, [23-13C]nor-chenodeoxycholic acid (nor-CDCA) had a pKa of 6.1, similar to that of CDCA (pKa 6.2), it...

    journal_title:Journal of lipid research

    pub_type: 杂志文章

    doi:

    authors: Ko J,Hamilton JA,Ton-Nu HT,Schteingart CD,Hofmann AF,Small DM

    更新日期:1994-05-01 00:00:00

  • Specific linoleate deficiency in the rat does not prevent substantial carbon recycling from [(14)C]linoleate into sterols.

    abstract::Compared with classic essential fatty acid deficiency or the feeding of a fat-free diet, little is known about specific linoleate deficiency in the rat. Carbon recycling into de novo lipogenesis has been reported to be an obligatory feature of linoleate metabolism in the liver, even in extreme linoleate deficiency (LA...

    journal_title:Journal of lipid research

    pub_type: 杂志文章

    doi:

    authors: Cunnane SC,Trotti D,Ryan MA

    更新日期:2000-11-01 00:00:00

  • ApoE enhances lipid uptake by macrophages in lipoprotein lipase deficiency during pregnancy.

    abstract::A woman with primary lipoprotein lipase (LPL) deficiency developed marked hypertriglyceridemia, pancreatitis, eruptive xanthomas, and unusual palmar xanthomas during pregnancy. Hypotheses to account for the palmar xanthomas were that oxidative modification of triglyceride (TG)-rich lipoproteins occurred due to increas...

    journal_title:Journal of lipid research

    pub_type: 杂志文章

    doi:

    authors: Steinberg FM,Tsai EC,Brunzell JD,Chait A

    更新日期:1996-05-01 00:00:00

  • Postprandial lipemia enhances the capacity of large HDL2 particles to mediate free cholesterol efflux via SR-BI and ABCG1 pathways in type IIB hyperlipidemia.

    abstract::Lipid and cholesterol metabolism in the postprandial phase is associated with both quantitative and qualitative remodeling of HDL particle subspecies that may influence their anti-atherogenic functions in the reverse cholesterol transport pathway. We evaluated the capacity of whole plasma or isolated HDL particles to ...

    journal_title:Journal of lipid research

    pub_type: 临床试验,杂志文章

    doi:10.1194/jlr.P009746

    authors: Julia Z,Duchene E,Fournier N,Bellanger N,Chapman MJ,Le Goff W,Guerin M

    更新日期:2010-11-01 00:00:00

  • Further studies of the effects of an anovulatory drug on lipid metabolism in the rat.

    abstract::The effect of various levels of the oral contraceptive drug, Enovid E, on serum and liver lipid levels of adult female rats has been investigated. Doses ranging from 0.052 to 1.04 mg/day have been employed in rats fed control or cholesterol-containing diets. It has been confirmed that after administration of even low,...

    journal_title:Journal of lipid research

    pub_type: 杂志文章

    doi:

    authors: Aftergood L,Alfin-Slater RB

    更新日期:1971-05-01 00:00:00

  • Preparation of lyso-GM1 (II3Neu5AcGgOse4-long chain bases) by a one-pot reaction.

    abstract::A simple procedure is described for preparing lyso-GM1, a GM1 derivative that lacks the fatty acid moiety, starting from GM1 ganglioside using a one-pot reaction. Ganglioside deacylation was carried out in KOH/propan-1-ol in the absence of oxygen. The yield of lyso-GM1 under optimal conditions (6 h, 90 degrees C, 0.2 ...

    journal_title:Journal of lipid research

    pub_type: 杂志文章

    doi:

    authors: Sonnino S,Acquotti D,Kirschner G,Uguaglianza A,Zecca L,Rubino F,Tettamanti G

    更新日期:1992-08-01 00:00:00

  • PIP4K2A regulates intracellular cholesterol transport through modulating PI(4,5)P2 homeostasis.

    abstract::The transport of LDL-derived cholesterol from lysosomes to peroxisomes is facilitated by membrane contacts formed between the lysosomal protein synaptotagmin VII and the peroxisomal lipid phosphatidylinositol 4, 5-bisphosphate [PI(4,5)P2]. Here, we used RNA interference to search for regulators of PI(4,5)P2 and to stu...

    journal_title:Journal of lipid research

    pub_type: 杂志文章

    doi:10.1194/jlr.M082149

    authors: Hu A,Zhao XT,Tu H,Xiao T,Fu T,Wang Y,Liu Y,Shi XJ,Luo J,Song BL

    更新日期:2018-03-01 00:00:00

  • Metabolic engineering of Pichia pastoris to produce ricinoleic acid, a hydroxy fatty acid of industrial importance.

    abstract::Ricinoleic acid (12-hydroxyoctadec-cis-9-enoic acid) has many specialized uses in bioproduct industries, while castor bean is currently the only commercial source for the fatty acid. This report describes metabolic engineering of a microbial system (Pichia pastoris) to produce ricinoleic acid using a "push" (synthesis...

    journal_title:Journal of lipid research

    pub_type: 杂志文章

    doi:10.1194/jlr.M060954

    authors: Meesapyodsuk D,Chen Y,Ng SH,Chen J,Qiu X

    更新日期:2015-11-01 00:00:00

  • Recruitment of cell phospholipids and cholesterol by apolipoproteins A-II and A-I: formation of nascent apolipoprotein-specific HDL that differ in size, phospholipid composition, and reactivity with LCAT.

    abstract::Studies were carried out to determine whether apolipoprotein (apo) A-II, like apoA-I, can recruit phospholipid and cholesterol from cell membranes, thereby forming nascent apoA-II-specific HDL. ApoA-II and apoA-I were purified from plasma and each was incubated with CHO cells at a concentration of 10 micrograms/ml. Li...

    journal_title:Journal of lipid research

    pub_type: 杂志文章

    doi:

    authors: Forte TM,Bielicki JK,Goth-Goldstein R,Selmek J,McCall MR

    更新日期:1995-01-01 00:00:00

  • Comparison of retinyl ester hydrolase activities in bovine liver and retinal pigment epithelium.

    abstract::Various properties of retinyl ester hydrolysis in the liver and the retinal pigment epithelium (RPE) have been studied, yet the relationship between the retinyl ester hydrolase (REH) activities in these tissues of the same species is not known. In the present study, REH activities in bovine liver and RPE microsomes we...

    journal_title:Journal of lipid research

    pub_type: 杂志文章

    doi:

    authors: Mata NL,Mata JR,Tsin AT

    更新日期:1996-09-01 00:00:00

  • Storage of vitamin A in extrahepatic stellate cells in normal rats.

    abstract::In mammals, vitamin A is primarily stored as retinyl esters in hepatic stellate cells under normal dietary intake of the vitamin. Previously, extrahepatic vitamin A-storing stellate cells have only been identified in animals maintained on a vitamin A-rich diet, and it has not been known whether these cells play a role...

    journal_title:Journal of lipid research

    pub_type: 杂志文章

    doi:

    authors: Nagy NE,Holven KB,Roos N,Senoo H,Kojima N,Norum KR,Blomhoff R

    更新日期:1997-04-01 00:00:00

  • Serum gangliosides in mice with metastatic and non-metastatic brain tumors.

    abstract::The content of serum gangliosides was examined in VM and C57BL/6J (B6) mice that contained subcutaneous metastatic (VM) and non-metastatic (CT-2A) brain tumors, respectively. Gas-liquid chromatography (GLC) and high performance thin-layer chromatography (HPTLC) were used to analyze the serum gangliosides. N-glycolylne...

    journal_title:Journal of lipid research

    pub_type: 杂志文章

    doi:

    authors: Cotterchio M,Seyfried TN

    更新日期:1994-01-01 00:00:00

  • Ascorbic acid and copper in linoleate oxidation. 3. Catalysts in combination.

    abstract::In promoting oxidation of 0.02 m potassium linoleate in a buffered (pH 7.0) aqueous dispersion at 37 degrees C, ascorbic acid at low concentrations (1.8 x 10(-6) and 1.8 x 10(-5) m) in combination with copper (1.3 x 10(-7) to 1.3 x 10(-3) m) had greater catalytic activity than the additive activity of the two catalyst...

    journal_title:Journal of lipid research

    pub_type: 杂志文章

    doi:

    authors: Haase G,Dunkley WL

    更新日期:1969-09-01 00:00:00

  • Cholesterol substrate pools and steroid hormone levels are normal in the face of mutational inactivation of NPC1 protein.

    abstract::Mutational inactivation of NPC1 largely blocks the movement of LDL-derived cholesterol from the lysosome to the metabolically active, cytosolic pool of sterol that is the substrate for steroid hormone production. Such a block might, in theory, lead to deficiencies in circulating levels of testosterone, progesterone, a...

    journal_title:Journal of lipid research

    pub_type: 杂志文章

    doi:10.1194/jlr.M500534-JLR200

    authors: Xie C,Richardson JA,Turley SD,Dietschy JM

    更新日期:2006-05-01 00:00:00

  • The 192R/Q polymorphs of serum paraoxonase PON1 differ in HDL binding, lipolactonase stimulation, and cholesterol efflux.

    abstract::Serum paraoxonase (PON1) is a HDL-associated enzyme exhibiting potentially antiatherogenic properties. Here, we examined the common PON1-192R/Q human polymorphism. Despite numerous studies, the effect of this polymorphism on the antiatherogenic potential of PON1 is yet unresolved. Our structural model suggests that am...

    journal_title:Journal of lipid research

    pub_type: 杂志文章

    doi:10.1194/jlr.M600297-JLR200

    authors: Gaidukov L,Rosenblat M,Aviram M,Tawfik DS

    更新日期:2006-11-01 00:00:00

  • Characterization of a new human apolipoprotein A-I Yame by direct sequencing of polymerase chain reaction-amplified DNA.

    abstract::A new genetic variant apolipoprotein (apo), A-I Yame, was discovered during screening for apoA-I genetic variants with isoelectric focusing gel electrophoresis. To investigate the structural abnormality of apoA-I Yame, we amplified the apoA-I gene isolated from the proband's peripheral blood leukocytes with the polyme...

    journal_title:Journal of lipid research

    pub_type: 杂志文章

    doi:

    authors: Takada Y,Sasaki J,Seki M,Ogata S,Teranishi Y,Arakawa K

    更新日期:1991-08-01 00:00:00

  • Modified spray for the detection of phospholipids on thin-layer chromatograms.

    abstract::A simplified method for the preparation of the Dittmer-Lester spray for the detection of phospholipids on thin-layer chromatograms is described. ...

    journal_title:Journal of lipid research

    pub_type: 杂志文章

    doi:

    authors: Vaskovsky VE,Kostetsky EY

    更新日期:1968-05-01 00:00:00

  • Hormonal regulation of the cholesterol 7 alpha-hydroxylase gene (CYP7).

    abstract::The transcriptional regulation of the rat cholesterol 7 alpha-hydroxylase gene (CYP7) by hormones and signal transduction pathways was studied by transient transfection assay of the promoter activity. HepG2 cells were transfected with deletion mutants of the CYP7 upstream region linked to the luciferase reporter gene....

    journal_title:Journal of lipid research

    pub_type: 杂志文章

    doi:

    authors: Crestani M,Stroup D,Chiang JY

    更新日期:1995-11-01 00:00:00

  • Adiponectin forms a complex with atherogenic LDL and inhibits its downstream effects.

    abstract::Adiponectin, an adipocyte-derived protein, has antiatherogenic and antidiabetic effects, but how it confers the atherogenic effects is not well known. To study the antiatherogenic mechanisms of adiponectin, we examined whether it interacts with atherogenic low density lipoprotein (LDL) to attenuate LDL's atherogenicit...

    journal_title:Journal of lipid research

    pub_type: 杂志文章

    doi:10.1194/jlr.RA120000767

    authors: Kakino A,Fujita Y,Ke LY,Chan HC,Tsai MH,Dai CY,Chen CH,Sawamura T

    更新日期:2020-11-21 00:00:00

  • Walnut-enriched diet increases the association of LDL from hypercholesterolemic men with human HepG2 cells.

    abstract::In a randomized, cross-over feeding trial involving 10 men with polygenic hypercholesterolemia, a control, Mediterranean-type cholesterol-lowering diet, and a diet of similar composition in which walnuts replaced approximately 35% of energy from unsaturated fat, were given for 6 weeks each. Compared with the control d...

    journal_title:Journal of lipid research

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:

    authors: Muñoz S,Merlos M,Zambón D,Rodríguez C,Sabaté J,Ros E,Laguna JC

    更新日期:2001-12-01 00:00:00

  • Drawbacks in the use of 23-nor-deoxycholic acid standards.

    abstract::23-Nor-deoxycholic acid is widely used as internal standard in gas-liquid chromatographic studies of bile acids. Two batches of this compound, submitted to conventional alkaline hydrolysis of bile acid conjugates, were found to be transformed into a product with chromatographic properties different from those of "auth...

    journal_title:Journal of lipid research

    pub_type: 杂志文章

    doi:

    authors: Attili AF,Angelico M,Capocaccia L,Pièche U,Cantafora A

    更新日期:1982-01-01 00:00:00

  • Archaebacterial ether lipid diversity analyzed by supercritical fluid chromatography: integration with a bacterial lipid protocol.

    abstract::A strategy has been developed for archaebacterial lipid analysis which provides three times the information to describe archaebacterial isolates and is compatible with simultaneous eubacterial/eukaryotic lipid analysis of environmental samples. Eubacterial and micro-eukaryotic biomass, community structure, and nutriti...

    journal_title:Journal of lipid research

    pub_type: 杂志文章

    doi:

    authors: Hedrick DB,Guckert JB,White DC

    更新日期:1991-04-01 00:00:00

  • Chylomicron apoprotein localization within rat intestinal epithelium: studies of normal and impaired lipid absorption.

    abstract::Monospecific antisera were produced to two chylomicron apoproteins (apoB, apoA-I) and utilized for indirect immunofluorescent localization of these apoproteins within rat intestinal epithelium during normal and impaired lipid absorption. Isolated intestinal epithelial cells prepared after different periods of lipid ab...

    journal_title:Journal of lipid research

    pub_type: 杂志文章

    doi:

    authors: Glickman RM,Kilgore A,Khorana J

    更新日期:1978-02-01 00:00:00

  • N-hexyl-O-glucosyl sphingosine, an inhibitor of glucosyl ceramide -glucosidase.

    abstract::A synthetic analog of glucocerebroside, N-hexyl-O-glucosyl sphingosine, was found to inhibit the glucosidase in rat spleen that hydrolyzes glucocerebroside. At a concentration of 1 micro m, the analog inhibited the enzyme by 48%. The mode of action appeared to be competitive, probably aided by tight binding of the ami...

    journal_title:Journal of lipid research

    pub_type: 杂志文章

    doi:

    authors: Erickson JS,Radin NS

    更新日期:1973-03-01 00:00:00

  • Differential turnover of polyunsaturated fatty acids in plasmalogen and diacyl glycerophospholipids of isolated cardiac myocytes.

    abstract::To investigate the relative turnover of esterified polyunsaturated fatty acids in diacylglycerophospholipids and plasmalogens in isolated cardiac myocytes, we characterized the phospholipid composition and distribution of radiolabel in different phospholipid classes and in individual molecular species of diradyl choli...

    journal_title:Journal of lipid research

    pub_type: 杂志文章

    doi:

    authors: DaTorre SD,Creer MH

    更新日期:1991-07-01 00:00:00