A method for determination of saturated phosphatidylcholine.

abstract：:Characterization of the chemical form and stereo-specificity of the fatty acid derivatives of arachidonic and linoleic acid in psoriatic epidermis is needed to define the enzymatic origin of these compounds and their possible role in pathogenesis. In an analysis of psoriatic skin scales, both free and esterified 13-hy...

journal_title：Journal of lipid research

authors： Baer AN,Costello PB,Green FA

更新日期：1990-01-01 00:00:00

abstract：:Conditions under which apolipoprotein (apo) A-I dissociates from human high density lipoproteins (HDL) during incubation in vitro have been investigated. Dissociation of apoA-I was demonstrated by non-denaturing gradient gel electrophoresis followed by immunoblotting for apoA-I and by size-exclusion chromatography. It...

journal_title：Journal of lipid research

authors： Liang HQ,Rye KA,Barter PJ

更新日期：1994-07-01 00:00:00

abstract：:It has been suggested that phytosterol and phytostanol esters possess similar cholesterol-lowering properties, however, whether mechanisms responsible are identical has not been addressed. To address this question, cholesterol plasma levels, absorption, biosynthesis, and turnover were measured in 15 hypercholesterolem...

journal_title：Journal of lipid research

authors： Jones PJ,Raeini-Sarjaz M,Ntanios FY,Vanstone CA,Feng JY,Parsons WE

更新日期：2000-05-01 00:00:00

abstract：:Rat liver microsomes were incubated with the monofunctional aminoreagent fluorescamine. Although the probe easily penetrated the membranes, two pools of phosphatidylethanolamine (PE) could be detected. The first pool rapidly reacted with the probe and comprised 80% of the total PE. The second pool exhibited a very slo...

journal_title：Journal of lipid research

authors： Valtersson C,Filipsson L,Dallner G

更新日期：1986-07-01 00:00:00

abstract：:A multiplexed assay was developed by MS to analyze, in a single run, six major human Apos involved in lipoprotein metabolism: ApoA-I, ApoA-II, ApoB100, ApoC-II, ApoC-III, and ApoE. This method was validated in vivo in six subjects who received a 14 h constant infusion of [5,5,5-(2)H3]L-leucine at 10 μM/kg/h. Plasma li...

journal_title：Journal of lipid research

authors： Croyal M,Fall F,Ferchaud-Roucher V,Chétiveaux M,Zaïr Y,Ouguerram K,Krempf M,Nobécourt E

更新日期：2016-03-01 00:00:00

abstract：:Although the synthesis pathways of intracellular triacylglycerol (TAG) species have been well elucidated, assessment of the contribution of an individual pathway to TAG pools in different mammalian organs, particularly under pathophysiological conditions, is difficult, although not impossible. Herein, we developed and...

journal_title：Journal of lipid research

authors： Han RH,Wang M,Fang X,Han X

更新日期：2013-04-01 00:00:00

abstract：:A variety of methods are currently used to analyze HL and LPL activities in mice. In search of a simple methodology, we analyzed mouse preheparin and postheparin plasma LPL and HL activities using specific polyclonal antibodies raised in rabbit against rat HL (anti-HL) and in goat against rat LPL (anti-LPL). As an alt...

journal_title：Journal of lipid research

authors： Dallinga-Thie GM,Zonneveld-de Boer AJ,van Vark-van der Zee LC,van Haperen R,van Gent T,Jansen H,De Crom R,van Tol A

更新日期：2007-12-01 00:00:00

abstract：:Membrane-protein interaction plays key roles in a wide variety of biological processes. Although various methods have been employed to measure membrane binding of soluble proteins, a robust high-throughput assay that is universally applicable to all proteins is lacking at present. Here we report a new fluorescence que...

journal_title：Journal of lipid research

authors： Kim H,Afsari HS,Cho W

更新日期：2013-12-01 00:00:00

abstract：:Atherogenic mixed dyslipidemia associates with oxidative stress and defective HDL antioxidative function in metabolic syndrome (MetS). The impact of statin treatment on the capacity of HDL to inactivate LDL-derived, redox-active phospholipid hydroperoxides (PCOOHs) in MetS is indeterminate. Insulin-resistant, hypertri...

journal_title：Journal of lipid research

authors： Orsoni A,Thérond P,Tan R,Giral P,Robillard P,Kontush A,Meikle PJ,Chapman MJ

更新日期：2016-11-01 00:00:00

abstract：:The tear film covers the anterior eye and the precise balance of its various constituting components is critical for maintaining ocular health. The composition of the tear film amphiphilic lipid sublayer, in particular, has largely remained a matter of contention due to the limiting concentrations of these lipid amphi...

journal_title：Journal of lipid research

authors： Lam SM,Tong L,Duan X,Petznick A,Wenk MR,Shui G

更新日期：2014-02-01 00:00:00

abstract：:Remnant-like emulsions were prepared with lipid compositions similar to remnants derived from triacylglycerol-rich lipoproteins. When injected into the bloodstream of conscious mice the remnant-like emulsions labeled with cholesteryl[13C]oleate were metabolized in the liver and the appearance of 13CO2 in the breath wa...

journal_title：Journal of lipid research

authors： Martins IJ,Redgrave TG

更新日期：1998-03-01 00:00:00

abstract：:The concentration dependence of the 500 MHz 1H-NMR spectra of taurocholate, taurochenodeoxycholate, taurodeoxycholate, and the monosulfate esters of taurochenodeoxycholate has been examined at 0.154 M NaCl in D2O. The resonances of the C18, C19, and C21 methyl groups and the C23 methylene group are differentially broa...

journal_title：Journal of lipid research

authors： Stevens RD,Ribeiro AA,Lack L,Killenberg PG

更新日期：1992-01-01 00:00:00

abstract：:A device for the simultaneous evaporation of six samples under separately controlled streams of nitrogen is described. ...

journal_title：Journal of lipid research

authors： Komarek RJ

更新日期：1967-05-01 00:00:00

abstract：:To study the mechanisms of hepatic HDL formation, we investigated the roles of ABCA1, ABCG1, and SR-BI in nascent HDL formation in primary hepatocytes isolated from mice deficient in ABCA1, ABCG1, or SR-BI and from wild-type (WT) mice. Under basal conditions, in WT hepatocytes, cholesterol efflux to exogenous apoA-I w...

journal_title：Journal of lipid research

authors： Ji A,Wroblewski JM,Cai L,de Beer MC,Webb NR,van der Westhuyzen DR

更新日期：2012-03-01 00:00:00

abstract：:We report a new genetic defect in the lecithin:cholesterol acyltransferase (LCAT) gene associated with classical clinical and biochemical features of fish eye disease. The 63-year-old Australian female proband also suffers from non-insulin-dependent (type II) diabetes mellitus. She presented with corneal opacities, ma...

journal_title：Journal of lipid research

authors： Contacos C,Sullivan DR,Rye KA,Funke H,Assmann G

更新日期：1996-01-01 00:00:00

abstract：:The effect of fat feeding on adipocyte insulin binding was examined to expand a study of adaptive changes in plasma membrane functions. Cells from rats fed a high fat (L) diet for five to seven days bound less insulin and showed a decreased response to insulin (glucose oxidation) compared to those from rats fed a high...

journal_title：Journal of lipid research

authors： Ip C,Tepperman HM,Holohan P,Tepperman J

更新日期：1976-11-01 00:00:00

abstract：:Previous studies have demonstrated that perturbations in barrier function stimulate epidermal lipid synthesis and that this increase can be prevented by occlusive membranes. These observations suggest that epidermal lipid synthesis might be related to barrier function and raised the question whether transcutaneous wat...

journal_title：Journal of lipid research

authors： Grubauer G,Elias PM,Feingold KR

更新日期：1989-03-01 00:00:00

abstract：:The low density lipoprotein receptor (LDLR) regulates the plasma cholesterol level by mediating endocytosis of LDL. We established stable hamster cell lines expressing two LDLRs with distinct functional defects, i.e., endocytosis and ligand binding. In the cell line expressing only I189D h/r (human-rat chimeric) LDLR,...

journal_title：Journal of lipid research

authors： Yoshida H,Yokode M,Yamamoto A,Masaki R,Murayama T,Horiuchi H,Kita T

更新日期：1999-05-01 00:00:00

abstract：:De novo synthesis and turnover of endogenous ceramide in cultured skin fibroblasts from patients affected with Farber lipogranulomatosis were studied by biosynthetical labeling of cellular sphingolipids with [14C]serine. The cellular uptake of [14C]serine and incorporation into de novo synthesized ceramide was similar...

journal_title：Journal of lipid research

authors： van Echten-Deckert G,Klein A,Linke T,Heinemann T,Weisgerber J,Sandhoff K

更新日期：1997-12-01 00:00:00

abstract：:Recently we evaluated an isotope ratio method (IRM) for measurement of cholesterol absorption in 14 patients (15 experiments) hospitalized in the metabolic ward by comparing it to simultaneous measurements with a fecal radioactivity method (FRM) and found good to excellent agreement between two procedures (Samuel, P.,...

journal_title：Journal of lipid research

authors： Samuel P,McNamara DJ,Ahrens EH Jr,Crouse JR,Parker T

更新日期：1982-03-01 00:00:00

abstract：:Phospholipase was isolated (in two fractions) from Vipera palestinae venom and it was shown to possess phospholipase A activity (hydrolyzing diacyl-sn-glycerophosphorylcholines, e.g., lecithin, in the 2-position) as well as lysophospholipase (phospholipase B) activity (hydrolyzing 1-monoacyl-sn-glycerophosphorylcholin...

journal_title：Journal of lipid research

authors： Shiloah J,Klibansky C,de Vries A,Berger A

更新日期：1973-05-01 00:00:00

abstract：:The genetic variability of apolipoprotein E (apoE) influences plasma lipoprotein levels, and allele frequencies differ between African Americans and Caucasians. As African Americans have higher lipoprotein [a] (Lp[a]) levels than Caucasians, we investigated the effects of the apoE gene on allele-specific apolipoprotei...

journal_title：Journal of lipid research

authors： Anuurad E,Lu G,Rubin J,Pearson TA,Berglund L

更新日期：2007-03-01 00:00:00

abstract：:Based on our previous observation that monoclonal antibody anti-apoD-4E11 reacted with several HDL proteins we studied them further with three questions in mind: i) is there common protein polymorphism in healthy individuals? ii) how many proteins are present and what are their characteristics? iii) are they all apoli...

journal_title：Journal of lipid research

authors： Camato R,Marcel YL,Milne RW,Lussier-Cacan S,Weech PK

更新日期：1989-06-01 00:00:00

abstract：:A method for apolipoprotein (apo) E genotyping was developed using the polymerase chain reaction (PCR) with allele-specific oligonucleotide primers (ASP). Synthetic oligonucleotides with base-pair mismatches at the 3' terminus were used as primers to amplify the apoE gene in subjects previously phenotyped using isoele...

journal_title：Journal of lipid research

authors： Main BF,Jones PJ,MacGillivray RT,Banfield DK

更新日期：1991-01-01 00:00:00

abstract：:Delta-5 and delta-6 desaturases (D5D and D6D) are key enzymes in endogenous synthesis of long-chain PUFAs. In this sample of healthy subjects (n = 310), genotypes of single nucleotide polymorphisms (SNPs) rs174537, rs174561, and rs3834458 in the FADS1-FADS2 gene cluster were strongly associated with proportions of LC-...

journal_title：Journal of lipid research

authors： Al-Hilal M,Alsaleh A,Maniou Z,Lewis FJ,Hall WL,Sanders TA,O'Dell SD,MARINA study team.

更新日期：2013-02-01 00:00:00

abstract：:ABCA12 mutations disrupt the skin barrier and cause harlequin ichthyosis. We previously showed Abca12(-/-) skin has increased glucosylceramide (GlcCer) and correspondingly lower amounts of ceramide (Cer). To examine why loss of ABCA12 leads to accumulation of GlcCer, de novo sphingolipid synthesis was assayed using [(...

journal_title：Journal of lipid research

authors： Haller JF,Cavallaro P,Hernandez NJ,Dolat L,Soscia SJ,Welti R,Grabowski GA,Fitzgerald ML,Freeman MW

更新日期：2014-03-01 00:00:00

abstract：:The ability to exogenously present cell-surface receptors in high-affinity conformations in a synthetic system offers an opportunity to provide host cells with protection from pathogenic toxins. This strategy requires improvement of the synthetic receptor binding affinity against its native counterpart, particularly w...

journal_title：Journal of lipid research

authors： Bricarello DA,Mills EJ,Petrlova J,Voss JC,Parikh AN

更新日期：2010-09-01 00:00:00

abstract：:3 alpha,7 alpha-dihydroxy-5 beta-cholestan-26-oic acid (DHCA) and 3 alpha,7 alpha,12 alpha-trihydroxy-5 beta-cholestan-26-oic acid (THCA) are metabolized into chenodeoxycholic acid and cholic acid, respectively, through oxidation and cleavage of the terminal three carbons of the side chain. The present study was desig...

journal_title：Journal of lipid research

authors： Cass OW,Williams GC,Hanson RF

更新日期：1980-02-01 00:00:00

abstract：:An elevated plasma concentration of lipoprotein (a) [Lp(a)] is an independent risk factor for cardiovascular disease. Life style modification and currently available drugs either fail to effectively lower plasma Lp(a) levels or do not result in clinical benefit. However, lipoprotein apheresis is very efficient in decr...

journal_title：Journal of lipid research

authors： Waldmann E,Parhofer KG

更新日期：2016-10-01 00:00:00

abstract：:The sialic acid present in erythrocyte ghosts was estimated by methane chemical ionization mass fragmentography of the trimethylsilyl methyl glycosides. The internal standard was 3,4,6-tris-trimethylsilyl-alpha-phenyl-2-deoxy-2-acetamide-D-glucosaminide, as proposed by Yu and Ledeen (J. Lipid Res. 1970. 11: 506-516). ...

journal_title：Journal of lipid research

authors： Ashraf J,Butterfield DA,Järnefelt J,Laine RA

更新日期：1980-11-01 00:00:00