Structural determinants for membrane association and dynamic organization of the hepatitis C virus NS3-4A complex.

abstract：:Fetal cerebral neurons at the initiation of active neurite outgrowth in culture incorporate 4-fold more [3H]glucosamine into glycoproteins than into the cellular lipid fraction. After 8 days or longer, when a well-developed fiber network is apparent, lipids are labeled more extensively than the glycoproteins. Labeling...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yavin E,Richter-Landsberg C,Duksin D,Yavin Z

更新日期：1984-09-01 00:00:00

abstract：:Analysis of the mRNA capping apparatus of the malaria parasite Plasmodium falciparum illuminates an evolutionary connection to fungi rather than metazoans. We show that P. falciparum encodes separate RNA guanylyltransferase (Pgt1) and RNA triphosphatase (Prt1) enzymes and that the triphosphatase component is a member ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ho CK,Shuman S

更新日期：2001-03-13 00:00:00

abstract：:Mate attraction in Aplysia involves a long-distance water-borne signal (the protein pheromone attractin), which is released during egg laying. Aplysia californica attractin attracts species that produce closely related attractins, such as Aplysia brasiliana, whose geographic distribution does not overlap that of A. ca...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Painter SD,Cummins SF,Nichols AE,Akalal DB,Schein CH,Braun W,Smith JS,Susswein AJ,Levy M,de Boer PA,ter Maat A,Miller MW,Scanlan C,Milberg RM,Sweedler JV,Nagle GT

更新日期：2004-05-04 00:00:00

abstract：:Costly interactions between species that arise as a by-product of ancestral similarities in communication signals are expected to persist only under specific evolutionary circumstances. Territorial aggression between species, for instance, is widely assumed to persist only when extrinsic barriers prevent niche diverge...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Drury JP,Cowen MC,Grether GF

更新日期：2020-06-09 00:00:00

abstract：:This paper shows that proteins display an unexpectedly wide range of behaviors in buffers containing moderate (0.1-10 mM) concentrations of SDS (complete unfolding, formation of stable intermediate states, specific association with SDS, and various kinetic phenomena); capillary electrophoresis provides a convenient me...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gudiksen KL,Gitlin I,Whitesides GM

更新日期：2006-05-23 00:00:00

abstract：:Fluorescein isothiocyanate (FITC) was used to label the rabbit intestinal brush border Na+-glucose carrier, identify the carrier protein on sodium dodecyl sulfate/polyacrylamide gel electrophoresis, and monitor the effect of ions and substrates on fluorescence quenching. Enriched brush border preparations were employe...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Peerce BE,Wright EM

更新日期：1984-04-01 00:00:00

abstract：:The earliest folding events in single-tryptophan mutants of RNase A were investigated by fluorescence measurements by using a combination of stopped-flow and continuous-flow mixing experiments covering the time range from 70 micros to 10 s. An ultrarapid double-jump mixing protocol was used to study refolding from an ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Welker E,Maki K,Shastry MC,Juminaga D,Bhat R,Scheraga HA,Roder H

更新日期：2004-12-21 00:00:00

abstract：:Reactive oxygen species, the by-products of oxidative energy metabolism, are considered a main proximate cause of aging. Accordingly, overexpression of the enzyme Cu-Zn superoxide dismutase 1 (SOD1) can lengthen lifespan of Drosophila melanogaster in the laboratory. However, the role of SOD1 as a main determinant of l...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Parker JD,Parker KM,Sohal BH,Sohal RS,Keller L

更新日期：2004-03-09 00:00:00

abstract：:The extinction of a species is inevitably preceded by the extirpation of a series of local populations. Ecological theory predicts that vulnerability to extirpation varies between populations and is ultimately linked to environmental heterogeneity. If populations of a species are present in multiple regions separated ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yackulic CB,Sanderson EW,Uriarte M

更新日期：2011-03-08 00:00:00

abstract：:Voltage-gated Na+ channels are the molecular targets of local anesthetics, class I antiarrhythmic drugs, and some anticonvulsants. These chemically diverse drugs inhibit Na+ channels with complex voltage- and frequency-dependent properties that reflect preferential drug binding to open and inactivated channel states. ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ragsdale DS,McPhee JC,Scheuer T,Catterall WA

更新日期：1996-08-20 00:00:00

abstract：:Thiopurine S-methyltransferase (TPMT) catalyzes the S-methylation of thiopurine drugs. TPMT genetic polymorphisms represent a striking example of the potential clinical value of pharmacogenetics. Subjects homozygous for TPMT*3A, the most common variant allele for low activity, an allele that encodes a protein with two...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wang L,Nguyen TV,McLaughlin RW,Sikkink LA,Ramirez-Alvarado M,Weinshilboum RM

更新日期：2005-06-28 00:00:00

abstract：:Mitochondrial protein kinases (mPKs) are molecular switches that down-regulate the oxidation of branched-chain alpha-ketoacids and pyruvate. Elevated levels of these metabolites are implicated in disease states such as insulin-resistant Type II diabetes, branched-chain ketoaciduria, and primary lactic acidosis. We rep...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Machius M,Chuang JL,Wynn RM,Tomchick DR,Chuang DT

更新日期：2001-09-25 00:00:00

abstract：:Strategies to treat cancer have focused primarily on the killing of tumor cells. Here, we describe a differential stress resistance (DSR) method that focuses instead on protecting the organism but not cancer cells against chemotherapy. Short-term starved S. cerevisiae or cells lacking proto-oncogene homologs were up t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Raffaghello L,Lee C,Safdie FM,Wei M,Madia F,Bianchi G,Longo VD

更新日期：2008-06-17 00:00:00

abstract：:Human cDNA clones encoding a structure-specific recognition protein, SSRP1, that binds specifically to DNA modified with cisplatin have been isolated and characterized. The SSRP1 gene maps to human chromosome 11q12. The cDNA clones, obtained by using partial-length cDNAs described previously, predict an 81-kDa protein...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bruhn SL,Pil PM,Essigmann JM,Housman DE,Lippard SJ

更新日期：1992-03-15 00:00:00

abstract：:Mutations in the gene encoding the endothelin receptor type B (EDNRB) produce congenital aganglionic megacolon and pigment abnormalities in mice and humans. Here we report a naturally occurring null mutation of the EDNRB gene in spotting lethal (sl) rats, which exhibit aganglionic megacolon associated with white coat ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gariepy CE,Cass DT,Yanagisawa M

更新日期：1996-01-23 00:00:00

abstract：:A panel of 20 recombinant Fab fragments reactive with the surface glycoprotein gp120 of human type 1 immunodeficiency virus (HIV-1) were examined for their ability to neutralize MN and IIIB strains of the virus. Neutralization was determined as the ability of the Fab fragments to inhibit infection as measured in both ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Barbas CF 3rd,Björling E,Chiodi F,Dunlop N,Cababa D,Jones TM,Zebedee SL,Persson MA,Nara PL,Norrby E

更新日期：1992-10-01 00:00:00

abstract：:When the Mn(2+) and Ca(2+) ions normally present in concanavalin A are removed, the protein becomes incapable of binding saccharides. To explore the structural differences between the native and demetallized forms and their effects on the saccharide-binding properties of the protein, we have refined and compared the c...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Reeke GN Jr,Becker JW,Edelman GM

更新日期：1978-05-01 00:00:00

abstract：:Early events in both T-cell receptor (CD3)- and CD4-induced signal transduction pathways include tyrosine phosphorylation of protein substrates, the generation of phosphatidylinositol-phosphate breakdown products, and the mobilization of intracellular Ca2+. Oxidative stress in T cells mediated by sulfhydryl-reactive n...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kanner SB,Kavanagh TJ,Grossmann A,Hu SL,Bolen JB,Rabinovitch PS,Ledbetter JA

更新日期：1992-01-01 00:00:00

abstract：:Six overlapping cosmid clones having an average insert size of 40 kilobase pairs were identified and isolated from a human genomic library by using a cDNA probe, Alu-7, specific for the amino acid sequence of C4d, a known region of the fourth component of human complement. Analysis of these genomic clones by restricti...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Carroll MC,Porter RR

更新日期：1983-01-01 00:00:00

abstract：:The largest known DNA viruses infect Acanthamoeba and belong to two markedly different families. The Megaviridae exhibit pseudo-icosahedral virions up to 0.7 μm in diameter and adenine-thymine (AT)-rich genomes of up to 1.25 Mb encoding a thousand proteins. Like their Mimivirus prototype discovered 10 y ago, they enti...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Legendre M,Bartoli J,Shmakova L,Jeudy S,Labadie K,Adrait A,Lescot M,Poirot O,Bertaux L,Bruley C,Couté Y,Rivkina E,Abergel C,Claverie JM

更新日期：2014-03-18 00:00:00

abstract：:Multiple sclerosis (MS) is a chronic inflammatory demyelinating disease of unknown cause that afflicts the central nervous system. MS is typified by a highly clonally restricted antigen-driven antibody response that is confined largely to the central nervous system. The major antigenic targets of this response and the...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Williamson RA,Burgoon MP,Owens GP,Ghausi O,Leclerc E,Firme L,Carlson S,Corboy J,Parren PW,Sanna PP,Gilden DH,Burton DR

更新日期：2001-02-13 00:00:00

abstract：:A fungal colony is a syncytium composed of a branched and interconnected network of cells. Chimerism endows colonies with increased virulence and ability to exploit nutritionally complex substrates. Moreover, chimera formation may be a driver for diversification at the species level by allowing lateral gene transfer b...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Roper M,Simonin A,Hickey PC,Leeder A,Glass NL

更新日期：2013-08-06 00:00:00

abstract：:Cellular membranes are a heterogeneous mix of lipids, proteins and small molecules. Special groupings enriched in saturated lipids and cholesterol form liquid-ordered domains, known as "lipid rafts," thought to serve as platforms for signaling, trafficking and material transport throughout the secretory pathway. Quest...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ursell TS,Klug WS,Phillips R

更新日期：2009-08-11 00:00:00

abstract：:Detailed molecular definition of the WAGR region at chromosome 11p13 has been achieved by chromosome breakpoint analysis and long-range restriction mapping. Here we describe the molecular detection of a cytogenetically invisible 1-megabase deletion in an individual with aniridia, cryptorchidism, and hypospadias but no...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： van Heyningen V,Bickmore WA,Seawright A,Fletcher JM,Maule J,Fekete G,Gessler M,Bruns GA,Huerre-Jeanpierre C,Junien C

更新日期：1990-07-01 00:00:00

abstract：:A biomimetic hydrogel platform was designed to signal encapsulated cells using immobilized cell-cell communication cues, with a focus on enhancing the survival and function of encapsulated pancreatic β-cells to treat type 1 diabetes. When MIN6 cells, a pancreatic β-cell line, were encapsulated in poly(ethylene glycol)...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lin CC,Anseth KS

更新日期：2011-04-19 00:00:00

abstract：:Mutational processes of simple sequence repeats (SSRs) in complex genomes are poorly understood. We examined these processes by introducing a two-phase mutation model. In this model, most mutations are single-step changes, but infrequent large jumps in repeat number also occur. We used computer simulations to determin...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Di Rienzo A,Peterson AC,Garza JC,Valdes AM,Slatkin M,Freimer NB

更新日期：1994-04-12 00:00:00

abstract：:Previously we reported that pretreatment of rats with the substance P (SP) antagonist CP-96,345 inhibits the enterotoxic responses following administration of toxin A from Clostridium difficile into ileal loops, indicating that SP participates in the intestinal responses to this toxin. We now report that injection of ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Castagliuolo I,Keates AC,Qiu B,Kelly CP,Nikulasson S,Leeman SE,Pothoulakis C

更新日期：1997-04-29 00:00:00

abstract：:The proapoptotic activity of the "BH3-only" molecule BAD can be differentially regulated by survival factor signaling. Bad-deficient mice lacking both BAD long and BAD short proteins proved viable, and most cell types appeared to develop normally. BAD did not exclusively account for cell death after withdrawal of surv...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ranger AM,Zha J,Harada H,Datta SR,Danial NN,Gilmore AP,Kutok JL,Le Beau MM,Greenberg ME,Korsmeyer SJ

更新日期：2003-08-05 00:00:00

abstract：:Cholera and enterotoxigenic Escherichia coli (ETEC) are among the most common causes of acute infantile gastroenteritis globally. We previously developed a rice-based vaccine that expressed cholera toxin B subunit (MucoRice-CTB) and had the advantages of being cold chain-free and providing protection against cholera t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tokuhara D,Yuki Y,Nochi T,Kodama T,Mejima M,Kurokawa S,Takahashi Y,Nanno M,Nakanishi U,Takaiwa F,Honda T,Kiyono H

更新日期：2010-05-11 00:00:00

abstract：:The formation of the past tense of verbs in English has been the focus of the debate concerning connectionist vs. symbolic accounts of language. Brain-injured patients differ with respect to whether they are more impaired in generating irregular past tenses (TAKE-TOOK) or past tenses for nonce verbs (WUG-WUGGED). Such...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Joanisse MF,Seidenberg MS

更新日期：1999-06-22 00:00:00