Abstract：
:Leucine-rich glioma inactivated 3 (LGI3) is a member of LGI/epitempin (EPTP) family. The biological function of LGI3 and its association with disease are not known. We previously reported that mouse LGI3 was highly expressed in brain in a developmentally and transcriptionally regulated manner. In this study, we identified syntaxin 1, a SNARE component in exocytosis, as a candidate functional target of LGI3. Western blot analysis of mouse brain extract with LGI3 antibodies detected multiple protein forms (75-, 60-, 35- and 25-kDa). Proteomic analysis, pull-down and coimmunoprecipitation experiments identified syntaxin 1 as an LGI3-associated protein. LGI3 colocalized with syntaxin 1 in processes of cortical neurons with punctate synaptic pattern and was enriched in synaptosomal fraction. Coimmunoprecipitation showed that LGI3-syntaxin 1 complex did not contain other SNARE components, SNAP25 and VAMP2. Recombinant LGI3 attenuated Ca(2+)-evoked glutamate release from digitonin-permeabilized synaptosomes and transfection of PC12 cells with LGI3 decreased K(+)-induced secretion of human growth hormone. Thus, LGI3 may play a regulatory role in neuronal exocytosis via its interaction with syntaxin 1.
journal_name
Neurosci Lettjournal_title
Neuroscience lettersauthors
Park WJ,Lee SE,Kwon NS,Baek KJ,Kim DS,Yun HYdoi
10.1016/j.neulet.2008.08.044subject
Has Abstractpub_date
2008-10-31 00:00:00pages
240-4issue
3eissn
0304-3940issn
1872-7972pii
S0304-3940(08)01142-7journal_volume
444pub_type
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journal_title：Neuroscience letters
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更新日期：2017-05-22 00:00:00
