当前位置： SCI文献检索 > PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA期刊下所有文献 > The kinesin-1 motor protein is regulated by a direct interaction of its head and tail.

The kinesin-1 motor protein is regulated by a direct interaction of its head and tail.

Abstract：

:Kinesin-1 is a molecular motor protein that transports cargo along microtubules. Inside cells, the vast majority of kinesin-1 is regulated to conserve ATP and to ensure its proper intracellular distribution and coordination with other molecular motors. Regulated kinesin-1 folds in half at a hinge in its coiled-coil stalk. Interactions between coiled-coil regions near the enzymatically active heads at the N terminus and the regulatory tails at the C terminus bring these globular elements in proximity and stabilize the folded conformation. However, it has remained a mystery how kinesin-1's microtubule-stimulated ATPase activity is regulated in this folded conformation. Here, we present evidence for a direct interaction between the kinesin-1 head and tail. We photochemically cross-linked heads and tails and produced an 8-A cryoEM reconstruction of the cross-linked head-tail complex on microtubules. These data demonstrate that a conserved essential regulatory element in the kinesin-1 tail interacts directly and specifically with the enzymatically critical Switch I region of the head. This interaction suggests a mechanism for tail-mediated regulation of the ATPase activity of kinesin-1. In our structure, the tail makes simultaneous contacts with the kinesin-1 head and the microtubule, suggesting the tail may both regulate kinesin-1 in solution and hold it in a paused state with high ADP affinity on microtubules. The interaction of the Switch I region of the kinesin-1 head with the tail is strikingly similar to the interactions of small GTPases with their regulators, indicating that other kinesin motors may share similar regulatory mechanisms.

journal_name

Proc Natl Acad Sci U S A

journal_title

Proceedings of the National Academy of Sciences of the United States of America

authors

Dietrich KA,Sindelar CV,Brewer PD,Downing KH,Cremo CR,Rice SE

doi

10.1073/pnas.0803575105

subject

Has Abstract

pub_date

2008-07-01 00:00:00

pages

8938-43

issue

26

eissn

0027-8424

issn

1091-6490

pii

0803575105

journal_volume

105

pub_type

杂志文章

相关文献

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA文献大全
  • Engineering bone tissue substitutes from human induced pluripotent stem cells.

    abstract：:Congenital defects, trauma, and disease can compromise the integrity and functionality of the skeletal system to the extent requiring implantation of bone grafts. Engineering of viable bone substitutes that can be personalized to meet specific clinical needs represents a promising therapeutic alternative. The aim of o...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1301190110

    authors： de Peppo GM,Marcos-Campos I,Kahler DJ,Alsalman D,Shang L,Vunjak-Novakovic G,Marolt D

    更新日期：2013-05-21 00:00:00

  • A genome-wide analysis of CpG dinucleotides in the human genome distinguishes two distinct classes of promoters.

    abstract：:A striking feature of the human genome is the dearth of CpG dinucleotides (CpGs) interrupted occasionally by CpG islands (CGIs), regions with relatively high content of the dinucleotide. CGIs are generally associated with promoters; genes, whose promoters are especially rich in CpG sequences, tend to be expressed in m...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0510310103

    authors： Saxonov S,Berg P,Brutlag DL

    更新日期：2006-01-31 00:00:00

  • Defective glycerol metabolism in aquaporin 9 (AQP9) knockout mice.

    abstract：:Aquaporin-9 (AQP9) is an aquaglyceroporin membrane channel shown biophysically to conduct water, glycerol, and other small solutes. Because the physiological role/s of AQP9 remain undefined and the expression sites of AQP9 remain incomplete and conflicting, we generated AQP9 knockout mice. In the absence of physiologi...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0610894104

    authors： Rojek AM,Skowronski MT,Füchtbauer EM,Füchtbauer AC,Fenton RA,Agre P,Frøkiaer J,Nielsen S

    更新日期：2007-02-27 00:00:00

  • Molecular cloning and nucleotide sequence of human glucocerebrosidase cDNA.

    abstract：:Mutations in the human glucocerebrosidase gene cause Gaucher disease. A cDNA clone containing the entire human glucocerebrosidase coding region from normal cells has been isolated using lambda gt11 expression libraries. The complete nucleotide sequence, a restriction map, and a hydropathy profile are presented. Hybrid...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.82.21.7289

    authors： Sorge J,West C,Westwood B,Beutler E

    更新日期：1985-11-01 00:00:00

  • Evidence for a significant urbanization effect on climate in China.

    abstract：:China has experienced rapid urbanization and dramatic economic growth since its reform process started in late 1978. In this article, we present evidence for a significant urbanization effect on climate based on analysis of impacts of land-use changes on surface temperature in southeast China, where rapid urbanization...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0400357101

    authors： Zhou L,Dickinson RE,Tian Y,Fang J,Li Q,Kaufmann RK,Tucker CJ,Myneni RB

    更新日期：2004-06-29 00:00:00

  • Increased cell proliferation, but not reduced cell death, induces lymphocytosis in bovine leukemia virus-infected sheep.

    abstract：:Lymphocyte homeostasis is the result of a critical balance between cell proliferation and death. Disruption of this subtle equilibrium can lead to the onset of leukemia, an increase in the number of lymphocytes being potentially due to both of these parameters. The relative importance of cell proliferation vs. apoptos...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.142100999

    authors： Debacq C,Asquith B,Kerkhofs P,Portetelle D,Burny A,Kettmann R,Willems L

    更新日期：2002-07-23 00:00:00

  • Primary structures of bovine liver low molecular weight kininogen precursors and their two mRNAs.

    abstract：:By using a mixture of synthetic oligodeoxyribonucleotides as a probe, cloned cDNA sequences specific for low molecular weight (LMW) kininogen have been isolated from a cDNA library of bovine liver mRNA sequences. Nucleotide sequence analyses of cloned cDNA inserts have revealed that bovine liver LMW kininogens are enc...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.80.1.90

    authors： Nawa H,Kitamura N,Hirose T,Asai M,Inayama S,Nakanishi S

    更新日期：1983-01-01 00:00:00

  • Intestinal brush border membrane Na+/glucose cotransporter functions in situ as a homotetramer.

    abstract：:The functional unit molecular size of the intestinal brush border membrane-bound Na+/glucose cotransporter was determined by radiation inactivation. Purified brush border membrane vesicles preserved in cryoprotectant buffer were irradiated (-135 degrees C) with high-energy electrons from a 13-MeV (1 eV = 1.602 x 10(-1...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.87.4.1456

    authors： Stevens BR,Fernandez A,Hirayama B,Wright EM,Kempner ES

    更新日期：1990-02-01 00:00:00

  • Molecular mechanism for differential recognition of membrane phosphatidylserine by the immune regulatory receptor Tim4.

    abstract：:Recognition of phosphatidylserine (PS) lipids exposed on the extracellular leaflet of plasma membranes is implicated in both apoptotic cell removal and immune regulation. The PS receptor T cell immunoglobulin and mucin-domain-containing molecule 4 (Tim4) regulates T-cell immunity via phagocytosis of both apoptotic (hi...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1320174111

    authors： Tietjen GT,Gong Z,Chen CH,Vargas E,Crooks JE,Cao KD,Heffern CT,Henderson JM,Meron M,Lin B,Roux B,Schlossman ML,Steck TL,Lee KY,Adams EJ

    更新日期：2014-04-15 00:00:00

  • Adverse childhood experiences and physiological wear-and-tear in midlife: Findings from the 1958 British birth cohort.

    abstract：:Allostatic load (AL) is a measure of overall physiological wear-and-tear over the life course, which could partially be the consequence of early life exposures. AL could allow a better understanding of the potential biological pathways playing a role in the construction of the social gradient in adult health. To explo...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1417325112

    authors： Barboza Solís C,Kelly-Irving M,Fantin R,Darnaudéry M,Torrisani J,Lang T,Delpierre C

    更新日期：2015-02-17 00:00:00

  • Building more accurate decision trees with the additive tree.

    abstract：:The expansion of machine learning to high-stakes application domains such as medicine, finance, and criminal justice, where making informed decisions requires clear understanding of the model, has increased the interest in interpretable machine learning. The widely used Classification and Regression Trees (CART) have ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1816748116

    authors： Luna JM,Gennatas ED,Ungar LH,Eaton E,Diffenderfer ES,Jensen ST,Simone CB 2nd,Friedman JH,Solberg TD,Valdes G

    更新日期：2019-10-01 00:00:00

  • The dicyclohexylcarbodiimide-binding protein c of ATP synthase from Escherichia coli is not sufficient to express an efficient H+ conduction.

    abstract：:Bacteriophage Mu was inserted into the unc genes of Escherichia coli. The resulting mutation AS12 had a polar effect on the unc operon: membranes of the mutant AS12 contained the dicyclohexylcarbodiimide-binding protein c and the protein a as sole subunits of the ATP synthase. It was shown by peptide mapping and amino...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.78.11.6643

    authors： Friedl P,Bienhaus G,Hoppe J,Schairer HU

    更新日期：1981-11-01 00:00:00

  • Mutant mice lacking the gamma isoform of protein kinase C show decreased behavioral actions of ethanol and altered function of gamma-aminobutyrate type A receptors.

    abstract：:Calcium/phospholipid-dependent protein kinase (protein kinase C, PKC) has been suggested to play a role in the sensitivity of gamma-aminobutyrate type A (GABAA) receptors to ethanol. We tested a line of null mutant mice that lacks the gamma isoform of PKC (PKC gamma) to determine the role of this brain-specific isoenz...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.92.9.3658

    authors： Harris RA,McQuilkin SJ,Paylor R,Abeliovich A,Tonegawa S,Wehner JM

    更新日期：1995-04-25 00:00:00

  • Erosion kinetics of hydrolytically degradable polymers.

    abstract：:Degradable polymers are beginning to play an increasing role as materials for environmental and medical applications. Understanding factors that control erosion, such as bond cleavage and the dissolution and diffusion of degradation products, will be critical to the future development of these materials. Erosion kinet...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.90.2.552

    authors： Tamada JA,Langer R

    更新日期：1993-01-15 00:00:00

  • A molecular switch that governs mitochondrial fusion and fission mediated by the BCL2-like protein CED-9 of Caenorhabditis elegans.

    abstract：:Depending on the cellular context, BCL2-like proteins promote mitochondrial fusion or fission. What determines which of these two opposing processes they promote has so far been unknown. Furthermore, the mechanisms through which BCL2-like proteins affect mitochondrial dynamics remain to be fully understood. The BCL2-l...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1103218108

    authors： Lu Y,Rolland SG,Conradt B

    更新日期：2011-10-11 00:00:00

  • Heterodimeric interactions among the 1-amino-cyclopropane-1-carboxylate synthase polypeptides encoded by the Arabidopsis gene family.

    abstract：:The pyridoxal phosphate-dependent enzyme, 1-aminocyclopropane-1-carboxylate synthase (ACS; EC 4.4.1.14), catalyzes the rate-limiting step in the ethylene biosynthetic pathway in plants. The Arabidopsis genome encodes nine ACS polypeptides that form eight functional (ACS2, ACS4-9, ACS11) and one nonfunctional (ACS1) ho...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0308515101

    authors： Tsuchisaka A,Theologis A

    更新日期：2004-02-24 00:00:00

  • Cytosynalin: a Mr 35,000 cytoskeleton-interacting and calmodulin-binding protein.

    abstract：:A Mr 35,000 calmodulin-binding protein, which also possesses the ability to interact with a number of cytoskeletal elements, was purified from bovine synaptosomal membranes, using hydroxylapatite and phosphocellulose column chromatography. We call this protein "cytosynalin," which is derived from a combination of the ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.84.7.1916

    authors： Sobue K,Okabe T,Kadowaki K,Itoh K,Tanaka T,Fujio Y

    更新日期：1987-04-01 00:00:00

  • A 200-kb region of human chromosome 22q11.2 confers antipsychotic-responsive behavioral abnormalities in mice.

    abstract：:Human chromosome 22q11.2 has been implicated in various behavioral abnormalities, including schizophrenia and other neuropsychiatric/behavioral disorders. However, the specific genes within 22q11.2 that contribute to these disorders are still poorly understood. Here, we show that an approximately 200-kb segment of hum...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0509635102

    authors： Hiroi N,Zhu H,Lee M,Funke B,Arai M,Itokawa M,Kucherlapati R,Morrow B,Sawamura T,Agatsuma S

    更新日期：2005-12-27 00:00:00

  • Model for eukaryotic tail-anchored protein binding based on the structure of Get3.

    abstract：:The Get3 ATPase directs the delivery of tail-anchored (TA) proteins to the endoplasmic reticulum (ER). TA-proteins are characterized by having a single transmembrane helix (TM) at their extreme C terminus and include many essential proteins, such as SNAREs, apoptosis factors, and protein translocation components. Thes...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0907522106

    authors： Suloway CJ,Chartron JW,Zaslaver M,Clemons WM Jr

    更新日期：2009-09-01 00:00:00

  • Are DNA spacers relics of gene amplification events?

    abstract：:The genome of a thermophilic fungus, Thermomyces lanuginosus, contains a tandemly arranged cluster of sequences that are approximately equal to 50% homologous with the cytoplasmic 5S RNA and that are selectable by hybridization techniques. Unlike typical pseudogenes, these sequences are not truncated; rather, they bea...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.81.6.1768

    authors： Wong WM,Abrahamson JL,Nazar RN

    更新日期：1984-03-01 00:00:00

  • Dematerialization: Variety, caution, and persistence.

    abstract：:Dematerialization, represented by declining consumption per GDP of energy or of goods, offers some hope for rising environmental quality with development. The declining proportion of income spent on staples as affluence grows, which income elasticity <1.0 measures, makes dematerialization widespread. Further, as learn...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0806099105

    authors： Ausubel JH,Waggoner PE

    更新日期：2008-09-02 00:00:00

  • Discovery and validation of small-molecule heat-shock protein 90 inhibitors through multimodality molecular imaging in living subjects.

    abstract：:Up-regulation of the folding machinery of the heat-shock protein 90 (Hsp90) chaperone protein is crucial for cancer progression. The two Hsp90 isoforms (α and β) play different roles in response to chemotherapy. To identify isoform-selective inhibitors of Hsp90(α/β)/cochaperone p23 interactions, we developed a dual-lu...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1205459109

    authors： Chan CT,Reeves RE,Geller R,Yaghoubi SS,Hoehne A,Solow-Cordero DE,Chiosis G,Massoud TF,Paulmurugan R,Gambhir SS

    更新日期：2012-09-11 00:00:00

  • Thyroid hormone modulates glucose production via a sympathetic pathway from the hypothalamic paraventricular nucleus to the liver.

    abstract：:Thyrotoxicosis increases endogenous glucose production (EGP) and induces hepatic insulin resistance. We have recently shown that these alterations can be modulated by selective hepatic sympathetic and parasympathetic denervation, pointing to neurally mediated effects of thyroid hormone on glucose metabolism. Here, we ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0805355106

    authors： Klieverik LP,Janssen SF,van Riel A,Foppen E,Bisschop PH,Serlie MJ,Boelen A,Ackermans MT,Sauerwein HP,Fliers E,Kalsbeek A

    更新日期：2009-04-07 00:00:00

  • Site-directed nanoparticle labeling of cytochrome c.

    abstract：:Although nanoparticle-protein conjugates have been synthesized for numerous applications, bioconjugation remains a challenge, often resulting in denaturation or loss of protein function. This is partly because the protein-nanoparticle interface is poorly understood, which impedes the use of nanoparticles in nanomedici...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0807299106

    authors： Aubin-Tam ME,Hwang W,Hamad-Schifferli K

    更新日期：2009-03-17 00:00:00

  • Retroviral analysis of cardiac morphogenesis: discontinuous formation of coronary vessels.

    abstract：:Cellular progenitors of the coronary vasculature are believed to enter the chicken heart during epicardial morphogenesis between stages 17 and 27 (days 3-5) of egg incubation. To trace cells which give rise to the coronary arteries in vivo, we applied retroviral cell tagging procedures and analyzed clonal populations ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.89.20.9504

    authors： Mikawa T,Fischman DA

    更新日期：1992-10-15 00:00:00

  • Cell separation using tilted-angle standing surface acoustic waves.

    abstract：:Separation of cells is a critical process for studying cell properties, disease diagnostics, and therapeutics. Cell sorting by acoustic waves offers a means to separate cells on the basis of their size and physical properties in a label-free, contactless, and biocompatible manner. The separation sensitivity and effici...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1413325111

    authors： Ding X,Peng Z,Lin SC,Geri M,Li S,Li P,Chen Y,Dao M,Suresh S,Huang TJ

    更新日期：2014-09-09 00:00:00

  • Water does not flow across the tight junctions of MDCK cell epithelium.

    abstract：:Although it has been known for decades that the tight junctions of fluid-transporting epithelia are leaky to ions, it has not been possible to determine directly whether significant transjunctional water movement also occurs. An optical microscopic technique was developed for the direct visualization of the flow veloc...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.95.11.6526

    authors： Kovbasnjuk O,Leader JP,Weinstein AM,Spring KR

    更新日期：1998-05-26 00:00:00

  • Inducible nitric oxide synthase expression inhibition by adenovirus E1A.

    abstract：:Nitric oxide (NO) is an antiviral effector of the innate immune system. Viruses that can interfere with NO synthesis may be able to replicate more rapidly than viruses that cannot limit NO synthesis. We show that the adenovirus E1A protein inhibits NO production by decreasing expression of the inducible NO synthase (N...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1337185100

    authors： Cao W,Bao C,Lowenstein CJ

    更新日期：2003-06-24 00:00:00

  • Nanomechanical properties of steric zipper globular structures.

    abstract：:The term amyloid defines a group of proteins that aggregate into plaques or fibers. Amyloid fibers gained their fame mostly due to their relation with neurodegenerative diseases in humans. However, secreted by lower organisms, such as bacteria and fungi, amyloid fibers play a functional role: for example, when they se...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1908782116

    authors： Lester-Zer N,Ghrayeb M,Chai L

    更新日期：2019-11-05 00:00:00

  • beta2-chimaerin is a novel target for diacylglycerol: binding properties and changes in subcellular localization mediated by ligand binding to its C1 domain.

    abstract：:The members of the chimaerin family of Rac-GTPase-activating proteins possess a single C1 domain with high homology to those present in protein kinase C (PKC) isozymes. This domain in PKCs is involved in phorbol ester and diacylglycerol (DAG) binding. We previously have demonstrated that one of the chimaerin isoforms,...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.96.21.11854

    authors： Caloca MJ,Garcia-Bermejo ML,Blumberg PM,Lewin NE,Kremmer E,Mischak H,Wang S,Nacro K,Bienfait B,Marquez VE,Kazanietz MG

    更新日期：1999-10-12 00:00:00