Transmembrane segment enhanced labeling as a tool for the backbone assignment of alpha-helical membrane proteins.

abstract：:The alternative splicing of mRNA is a critical process in higher eukaryotes that generates substantial proteomic diversity. Many of the proteins that are essential to this process contain arginine/serine-rich (RS) domains. ZRANB2 is a widely-expressed and highly-conserved RS-domain protein that can regulate alternativ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Loughlin FE,Mansfield RE,Vaz PM,McGrath AP,Setiyaputra S,Gamsjaeger R,Chen ES,Morris BJ,Guss JM,Mackay JP

更新日期：2009-04-07 00:00:00

abstract：:Protein kinases play key roles in the induction by human interferon alpha (IFN-alpha) of specific gene expression and biological activity in various human cell lines. We now report that IFN-alpha increased the 7-kb transcript for the epsilon isotype of protein kinase C (PKC-epsilon) and the cellular content of PKC-eps...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wang C,Constantinescu SN,MacEwan DJ,Strulovici B,Dekker LV,Parker PJ,Pfeffer LM

更新日期：1993-08-01 00:00:00

abstract：:Queuosine (Q) is a complex tRNA modification widespread in eukaryotes and bacteria that contributes to the efficiency and accuracy of protein synthesis. Eukaryotes are not capable of Q synthesis and rely on salvage of the queuine base (q) as a Q precursor. While many bacteria are capable of Q de novo synthesis, salvag...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yuan Y,Zallot R,Grove TL,Payan DJ,Martin-Verstraete I,Šepić S,Balamkundu S,Neelakandan R,Gadi VK,Liu CF,Swairjo MA,Dedon PC,Almo SC,Gerlt JA,de Crécy-Lagard V

更新日期：2019-09-17 00:00:00

abstract：:Actin-myosin subfragment-1 (SF-1) or actin-heavy meromyosin is dissociated by the binding of ADP and vanadate (Vi) under conditions such that ADP alone does not dissociate the complex. The association constant of the stable complex M.ADP.Vi, in which M indicates myosin [Goodno, C. C. (1979) Proc. Natl. Acad. Sci. USA ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Goodno CC,Taylor EW

更新日期：1982-01-01 00:00:00

abstract：:The Na,K-ATPase contains a binding site for cardiac glycosides, such as ouabain, digoxin, and digitoxin, which is highly conserved among species ranging from Drosophila to humans. Although advantage has been taken of this site to treat congestive heart failure with drugs such as digoxin, it is unknown whether this sit...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dostanic-Larson I,Van Huysse JW,Lorenz JN,Lingrel JB

更新日期：2005-11-01 00:00:00

abstract：:Defining the processes of autoimmune attack of tissues is important for inhibiting continued tissue destruction. In type 1 diabetes, it is not known how cytotoxic effector T cell responses evolve over time in the pancreatic islets targeted for destruction. We used two-photon microscopy of live, intact, individual isle...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Friedman RS,Lindsay RS,Lilly JK,Nguyen V,Sorensen CM,Jacobelli J,Krummel MF

更新日期：2014-06-24 00:00:00

abstract：:B7 family proteins provide costimulatory signals that regulate T cell responses. Here we report the third set of B7 family-related T cell inhibitory molecules with the identification of a homolog of the B7 family, B7x. It is expressed in immune cells, nonlymphoid tissues, and some tumor cell lines. B7x inhibits cell-c...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zang X,Loke P,Kim J,Murphy K,Waitz R,Allison JP

更新日期：2003-09-02 00:00:00

abstract：:Calcific aortic valve disease is a chronic inflammatory process, and aortic valve interstitial cells (AVICs) from diseased aortic valves express greater levels of osteogenic factors in response to proinflammatory stimulation. Here, we report that lower cellular levels of IL-37 in AVICs of diseased human aortic valves ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zeng Q,Song R,Fullerton DA,Ao L,Zhai Y,Li S,Ballak DB,Cleveland JC Jr,Reece TB,McKinsey TA,Xu D,Dinarello CA,Meng X

更新日期：2017-02-14 00:00:00

abstract：:Natural environments typically contain multiple sound sources. The sounds from these sources frequently overlap in time and often mask each other. Masking could potentially distort the representation of a sound's spectrum, altering its timbre and impairing object recognition. Here, we report that the auditory system p...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： McDermott JH,Oxenham AJ

更新日期：2008-04-15 00:00:00

abstract：:The plasmid construct pSVO-CAT has been used to test adenovirus promoter activities in the unmethylated or methylated state. We have now observed that the E2A late promoter of adenovirus type 2 (Ad2) DNA also activated the chloramphenicol acetyltransferase (CAT) gene upon transfection of the pAd2E2A-CAT construct into...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Langner KD,Weyer U,Doerfler W

更新日期：1986-03-01 00:00:00

abstract：:Numbers of substitutions per site for 15 protein-coding genes and six introns of the plant mitochondria were estimated to compare modes and tempos of evolution between exons and introns, and numbers of insertions-deletions per site also were investigated in introns. Intra-gene homogeneity of numbers of substitutions p...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Laroche J,Li P,Maggia L,Bousquet J

更新日期：1997-05-27 00:00:00

abstract：:The chemical dynamics of lakes and streams affect their suitability as aquatic habitats and as water supplies for human needs. Because water quality is typically monitored only weekly or monthly, however, the higher-frequency dynamics of stream chemistry have remained largely invisible. To illuminate a wider spectrum ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kirchner JW,Neal C

更新日期：2013-07-23 00:00:00

abstract：:The sex pheromone biosynthetic pathways of lepidopterans require the concerted actions of multiple gene products. A number of pheromone gland (PG)-specific genes have been cloned in recent years and, whereas in vitro characterizations have indicated functions consistent with roles in pheromone production, there have b...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ohnishi A,Hull JJ,Matsumoto S

更新日期：2006-03-21 00:00:00

abstract：:The chief impediment to the precise biochemical identification of transfer factor and its mechanism of action has been the lack of a reproducible in vitro assay. We now report on a method by which dialysates containing transfer factor of proven in vivo potency can convert nonimmune lymphocytes to immune responsiveness...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ascher MS,Schneider WJ,Valentine FT,Lawrence HS

更新日期：1974-04-01 00:00:00

abstract：:A cDNA library derived from human cerebral cortex was screened for the presence of sodium channel alpha subunit-specific clones. Ligation of three overlapping clones generated a full-length cDNA clone, HBA, that provided the complete nucleotide sequence coding for a protein of 2005 amino acids. The predicted structure...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ahmed CM,Ware DH,Lee SC,Patten CD,Ferrer-Montiel AV,Schinder AF,McPherson JD,Wagner-McPherson CB,Wasmuth JJ,Evans GA

更新日期：1992-09-01 00:00:00

abstract：:Several disulfide benzamides have been shown to possess wide-spectrum antiretroviral activity in cell culture at low micromolar to submicromolar concentrations, inhibiting human immunodeficiency virus (HIV) type 1 (HIV-1) clinical and drug-resistant strains along with HIV-2 and simian immunodeficiency virus [Rice, W. ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tummino PJ,Scholten JD,Harvey PJ,Holler TP,Maloney L,Gogliotti R,Domagala J,Hupe D

更新日期：1996-02-06 00:00:00

abstract：:Several cDNA clones encoding the human immunoglobulin G receptor CD16 were isolated from human lung or peripheral blood leukocyte cDNA libraries. Nucleotide sequence comparisons revealed that the cDNAs could be divided into two groups. cDNA clones in one group encode a protein that terminates 4 amino acids after the p...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Scallon BJ,Scigliano E,Freedman VH,Miedel MC,Pan YC,Unkeless JC,Kochan JP

更新日期：1989-07-01 00:00:00

abstract：:An important goal in biology is to predict from sequence data the high-resolution structures of proteins and the interactions that occur between them. In this paper, we describe a computational approach that can make these types of predictions for a series of coiled-coil dimers. Our method comprises a dual strategy th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Keating AE,Malashkevich VN,Tidor B,Kim PS

更新日期：2001-12-18 00:00:00

abstract：:High-valent intermediates of binuclear nonheme iron enzymes are structurally unknown despite their importance for understanding enzyme reactivity. Nuclear resonance vibrational spectroscopy combined with density functional theory calculations has been applied to structurally well-characterized high-valent mono- and di...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Park K,Bell CB 3rd,Liu LV,Wang D,Xue G,Kwak Y,Wong SD,Light KM,Zhao J,Alp EE,Yoda Y,Saito M,Kobayashi Y,Ohta T,Seto M,Que L Jr,Solomon EI

更新日期：2013-04-16 00:00:00

abstract：:alpha-1-Antitrypsin is a serum protein that inhibits many proteolytic enzymes. Recently, it was suggested that the alpha-1-antitrypsin-trypsin complex is an acyl ester analogous to the acyl intermediate that forms between trypsin and its substrates. In previous work we showed that the alpha-1-antitrypsin-trypsin compl...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cohen AB,Gruenke LD,Craig JC,Geczy D

更新日期：1977-10-01 00:00:00

abstract：:Many processes in science and engineering develop multiscale temporal and spatial patterns, with complex underlying dynamics and time-dependent external forcings. Because of the importance in understanding and predicting these phenomena, extracting the salient modes of variability empirically from incomplete observati...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Giannakis D,Majda AJ

更新日期：2012-02-14 00:00:00

abstract：:We investigate the roles of the pseudospin and the valley degeneracy in electron scattering at graphene edges. It is found that they are strongly correlated with charge density modulations of short-wavelength oscillations and slowly decaying beat patterns in the electronic density profile. Theoretical analyses using n...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Park C,Yang H,Mayne AJ,Dujardin G,Seo S,Kuk Y,Ihm J,Kim G

更新日期：2011-11-15 00:00:00

abstract：:The estrogen receptor (E2R) from rabbit uterus has an enhanced affinity for BrdUrd-substituted DNA compared to unsubstituted DNA. Increasing levels of BrdUrd substitution (from 0 to 100%) in a DNA sample are associated with increasingly tighter binding of E2R to that DNA as measured by equilibrium competition experime...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kallos J,Fasy TM,Hollander VP,Bick MD

更新日期：1978-10-01 00:00:00

abstract：:For a distribution F on R(p) and a point x in R(p) the simplicial depth D(x), which is the probability that x be inside a random simplex whose vertices are p + 1 independent observations from F, is introduced. D(x) can be viewed as a measure of depth of the point x relative to F, and its empirical version gives rise t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Liu RY

更新日期：1988-03-01 00:00:00

abstract：:We identified cell surface markers associated with repression of p16(INK4a)/cyclin-dependent kinase inhibitor 2A(CDKN2A), a critical determinant in the acquisition of a plastic state. These cell surface markers allowed direct isolation of rare cells from healthy human breast tissue that exhibit extensive lineage plast...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Roy S,Gascard P,Dumont N,Zhao J,Pan D,Petrie S,Margeta M,Tlsty TD

更新日期：2013-03-19 00:00:00

abstract：:The allocation of carbon and nitrogen resources to the synthesis of plant proteins, carbohydrates, and lipids is complex and under the control of many genes; much remains to be understood about this process. QQS (Qua-Quine Starch; At3g30720), an orphan gene unique to Arabidopsis thaliana, regulates metabolic processes...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Li L,Zheng W,Zhu Y,Ye H,Tang B,Arendsee ZW,Jones D,Li R,Ortiz D,Zhao X,Du C,Nettleton D,Scott MP,Salas-Fernandez MG,Yin Y,Wurtele ES

更新日期：2015-11-24 00:00:00

abstract：:Synaptic vesicles release neurotransmitter by following a process of vesicle docking and exocytosis. Although these steps are well established, it has been difficult to observe and measure these rates directly in living synapses. Here, by combining the direct imaging of single synaptic vesicles and synaptic ribbons, I...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zenisek D

更新日期：2008-03-25 00:00:00

abstract：:Peptidoglycan (PGN) recognition proteins (PGRPs) are pattern-recognition receptors of the innate immune system that bind and, in some cases, hydrolyze bacterial PGNs. We determined the crystal structure, at 2.30-A resolution, of the C-terminal PGN-binding domain of human PGRP-Ialpha in complex with a muramyl tripeptid...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Guan R,Roychowdhury A,Ember B,Kumar S,Boons GJ,Mariuzza RA

更新日期：2004-12-07 00:00:00

abstract：:Developmental constraint is indicated when one finds that similar genetic mechanisms are responsible for independent origins of the same derived phenotype. We studied three independent origins of rosette flowering within the mustard family and attempted to evaluate the extent to which the same mechanisms were involved...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yoon HS,Baum DA

更新日期：2004-04-27 00:00:00

abstract：:Human tyrosyl-DNA phosphodiesterase (TDP1) hydrolyzes the phosphodiester bond at a DNA 3'-end linked to a tyrosyl moiety and has been implicated in the repair of topoisomerase I (Top1)-DNA covalent complexes. TDP1 can also hydrolyze other 3'-end DNA alterations including 3'-phosphoglycolate and 3'-abasic sites, and ex...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Das BB,Dexheimer TS,Maddali K,Pommier Y

更新日期：2010-11-16 00:00:00