In vitro properties of leukocyte dialysates containing transfer factor.

abstract：:Ubiquitination, the crucial posttranslational modification that regulates the eukaryotic proteome, is carried out by a trio of enzymes, known as E1 [ubiquitin (Ub)-activating enzyme], E2 (Ub-conjugating enzyme), and E3 (Ub ligase). Although most E2s can work with any of the three mechanistically distinct classes of E3...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dove KK,Kemp HA,Di Bona KR,Reiter KH,Milburn LJ,Camacho D,Fay DS,Miller DL,Klevit RE

更新日期：2017-08-08 00:00:00

abstract：:The influence of the rare initiation triplet AUU on mRNA translation was investigated by comparing the activity of two pairs of model mRNAs that differ in the length of Shine-Dalgarno and spacer sequences. Irrespective of the initiation triplet (AUG or AUU), all mRNAs had similar template activity in vitro, but transl...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： La Teana A,Pon CL,Gualerzi CO

更新日期：1993-05-01 00:00:00

abstract：:Mutant I1A cells, lacking IL-1 receptor-associated kinase (IRAK) mRNA and protein, have been used to study the involvement of IRAK in NFkappaB and c-Jun N-terminal kinase (JNK) activation. A series of IRAK deletion constructs were expressed in I1A cells, which were then tested for their ability to respond to IL-1. Bot...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Li X,Commane M,Jiang Z,Stark GR

更新日期：2001-04-10 00:00:00

abstract：:We have used a motif-based structural search method to identify structural homologs of the hormone binding domains of the nuclear receptors from among a set of known protein structures and have found the closest similarity with members of the subtilisin-like serine proteases. These proteins consist of an open twisted ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Goldstein RA,Katzenellenbogen JA,Luthey-Schulten ZA,Seielstad DA,Wolynes PG

更新日期：1993-11-01 00:00:00

abstract：:By analogy with receptor-mediated endocytosis, clathrin-coated membrane segments at locations other than at the plasma membrane have been implicated in intracellular membrane transport/sorting. The crucial role of the Golgi apparatus in these processes prompted us to study the distribution at this level of clathrin-im...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Orci L,Ravazzola M,Amherdt M,Louvard D,Perrelet A

更新日期：1985-08-01 00:00:00

abstract：:Endothelial cells (ECs) not only are important for oxygen delivery but also act as a paracrine source for signals that determine the balance between tissue regeneration and fibrosis. Here we show that genetic inactivation of flow-induced transcription factor Krüppel-like factor 2 (KLF2) in ECs results in reduced liver...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Manavski Y,Abel T,Hu J,Kleinlützum D,Buchholz CJ,Belz C,Augustin HG,Boon RA,Dimmeler S

更新日期：2017-04-11 00:00:00

abstract：:Voltage-gated Na+ channels are the molecular targets of local anesthetics, class I antiarrhythmic drugs, and some anticonvulsants. These chemically diverse drugs inhibit Na+ channels with complex voltage- and frequency-dependent properties that reflect preferential drug binding to open and inactivated channel states. ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ragsdale DS,McPhee JC,Scheuer T,Catterall WA

更新日期：1996-08-20 00:00:00

abstract：:AB5 bacterial toxins and polyomaviruses induce membrane curvature as a mechanism to facilitate their entry into host cells. How membrane bending is accomplished is not yet fully understood but has been linked to the simultaneous binding of the pentameric B subunit to multiple copies of glycosphingolipid receptors. Her...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kabbani AM,Raghunathan K,Lencer WI,Kenworthy AK,Kelly CV

更新日期：2020-06-30 00:00:00

abstract：:The [A] allele of SNP rs965513 in 9q22 has been consistently shown to be highly associated with increased papillary thyroid cancer (PTC) risk with an odds ratio of ∼1.8 as determined by genome-wide association studies, yet the molecular mechanisms remain poorly understood. Previously, we noted that the expression of t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： He H,Li W,Liyanarachchi S,Srinivas M,Wang Y,Akagi K,Wang Y,Wu D,Wang Q,Jin V,Symer DE,Shen R,Phay J,Nagy R,de la Chapelle A

更新日期：2015-05-12 00:00:00

abstract：:To test directly whether fibrin(ogen) is a key binding site for apolipoprotein(a) [apo(a)] in vessel walls, apo(a) transgenic mice and fibrinogen knockout mice were crossed to generate fibrin(ogen)-deficient apo(a) transgenic mice and control mice. In the vessel wall of apo(a) transgenic mice, fibrin(ogen) deposition ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lou XJ,Boonmark NW,Horrigan FT,Degen JL,Lawn RM

更新日期：1998-10-13 00:00:00

abstract：:All organisms contain thioredoxin (TRX), a regulatory thiol:disulfide protein that reduces disulfide bonds in target proteins. Unlike animals and yeast, plants contain numerous TRXs for which no function has been assigned in vivo. Recent in vitro proteomic approaches have opened the way to the identification of >100 T...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Vignols F,Bréhélin C,Surdin-Kerjan Y,Thomas D,Meyer Y

更新日期：2005-11-15 00:00:00

abstract：:We have isolated and analyzed a cDNA from the central nervous system of the mollusc Lymnaea stagnalis encoding a putative receptor, which might be a natural hybrid between two different classes of receptor proteins. Preceded by a signal peptide, two types of repeated sequences are present in the N-terminal part of the...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tensen CP,Van Kesteren ER,Planta RJ,Cox KJ,Burke JF,van Heerikhuizen H,Vreugdenhil E

更新日期：1994-05-24 00:00:00

abstract：:DNA polymerases from Xenopus laevis oocytes, unfertilized eggs, and kidney cells grown in culture have been characterized. The same three major DNA polymerase activities are present in all cell types examined. We attempt to relate the characteristics of amphibian enzymes to those of mammalian polymerases. ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tatò F,Gandini DA,Tocchini-Valentini GP

更新日期：1974-09-01 00:00:00

abstract：:The involvement of p53 in regulating diverse cellular processes dictates that it must respond to multiple signaling mechanisms, thus coordinating the response to various "stress conditions." Genotoxic stress has served as a paradigm to dissect the transactivation-dependent branch of the pathway by which p53 can induce...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ruaro EM,Collavin L,Del Sal G,Haffner R,Oren M,Levine AJ,Schneider C

更新日期：1997-04-29 00:00:00

abstract：:Jun is a transcription factor belonging to the activator protein 1 family. A mutated version of Jun (v-Jun) transduced by the avian retrovirus ASV17 induces oncogenic transformation in avian cell cultures and sarcomas in young galliform birds. The oncogenicity of Jun probably results from transcriptional deregulation ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fu Sl,Bottoli I,Goller M,Vogt PK

更新日期：1999-05-11 00:00:00

abstract：:The Tsc2 gene, which is mutationally inactivated in the germ line of some families with tuberous sclerosis, encodes a large, membrane-associated GTPase activating protein (GAP) designated tuberin. Studies of the Eker rat model of hereditary cancer strongly support the role of Tsc2 as a tumor suppressor gene. In this s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jin F,Wienecke R,Xiao GH,Maize JC Jr,DeClue JE,Yeung RS

更新日期：1996-08-20 00:00:00

abstract：:Although anatomy is often the first step in assigning functions to neural structures, it is not always clear whether architecturally distinct regions of the brain correspond to operational units. Whereas neuroarchitecture remains relatively static, functional connectivity may change almost instantaneously according to...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Weir PT,Dickinson MH

更新日期：2015-10-06 00:00:00

abstract：:We have studied the kinetics of vitellogenin mRNA accumulation in rooster liver after a primary injection of 17beta-estradiol. The levels of vitellogenin mRNA have been determined both by hybridization of total cellular RNA to vitellogenin cDNA and by translation of vitellogenin mRNA in a wheat germ cell-free system. ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Burns AT,Deeley RG,Gordon JI,Udell DS,Mullinix KP,Goldberger RF

更新日期：1978-04-01 00:00:00

abstract：:A hypothetical gene with similarity to the ispD gene of Escherichia coli was cloned from Arabidopsis thaliana cDNA. The ORF of 909 bp specifies a protein of 302 amino acid residues. The cognate chromosomal gene consists of 2,071 bp and comprises 11 introns with a size range of 78-202 bp. A fragment comprising amino ac...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rohdich F,Wungsintaweekul J,Eisenreich W,Richter G,Schuhr CA,Hecht S,Zenk MH,Bacher A

更新日期：2000-06-06 00:00:00

abstract：:Caenorhabditis elegans explores its environment by interrupting its forward movement with occasional turns and reversals. Turns and reversals occur at stable frequencies but irregular intervals, producing probabilistic exploratory behaviors. Here we dissect the roles of individual sensory neurons, interneurons, and mo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gray JM,Hill JJ,Bargmann CI

更新日期：2005-03-01 00:00:00

abstract：:In the growth plate of endochondral bones, parathyroid hormone (PTH)-related peptide (PTHrP) regulates the rate of chondrocyte maturation from prehypertrophic chondrocytes to hypertrophic chondrocytes. Using an antibody specific for Sox9 phosphorylated at serine 181 (S(181)), one of the two consensus protein kinase A ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Huang W,Chung UI,Kronenberg HM,de Crombrugghe B

更新日期：2001-01-02 00:00:00

abstract：:Contractile forces are essential for many developmental processes involving cell shape change and tissue deformation. Recent experiments on reconstituted actomyosin networks, the major component of the contractile machinery, have shown that active contractility occurs above a threshold motor concentration and within a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wang S,Wolynes PG

更新日期：2012-04-24 00:00:00

abstract：:Site-specific recombination is responsible for a broad range of biological phenomena, including DNA inversion, resolution of transposition intermediates, and the integration and excision of bacteriophage genomes. Integration of mycobacteriophage L5 is catalyzed by a phage-encoded integrase with recombination occurring...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Peña CE,Kahlenberg JM,Hatfull GF

更新日期：2000-07-05 00:00:00

abstract：:Serotonin (5-hydroxytryptamine; 0.5 microM and above) stimulated the synthesis of prostacyclin (as measured by radioimmunoassay of 6-ketoprostaglandin F1 alpha) by bovine aortic smooth muscle cells in culture. This effect was structurally specific; a similar response was not elicited by the other indoles (tryptophan, ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Coughlin SR,Moskowitz MA,Antoniades HN,Levine L

更新日期：1981-11-01 00:00:00

abstract：:Transcription, splicing, and translation are potentially coordinately regulatable in a temporospatial-dependent manner, although supporting experimental evidence for this notion is scarce. Yeast two-hybrid screening of a mammary gland cDNA library with human p21-activated kinase 1 (Pak1) as bait identified polyC-RNA-b...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Meng Q,Rayala SK,Gururaj AE,Talukder AH,O'Malley BW,Kumar R

更新日期：2007-04-03 00:00:00

abstract：:Aposematic coloration, or warning coloration, is a visual signal that acts to minimize contact between predator and unprofitable prey. The conditions favoring the evolution of aposematic coloration remain largely unidentified. Recent work suggests that diet specialization and resultant toxicity may play a role in faci...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Prudic KL,Oliver JC,Sperling FA

更新日期：2007-12-04 00:00:00

abstract：:The Hsp90 chaperone buffers development against a wide range of morphological changes in many organisms and in Drosophila masks the effects of hidden genetic variation. Theory predicts that genetic and nongenetic buffering will share common mechanisms. For example, it is argued that Hsp90 genetic buffering evolved sol...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Milton CC,Huynh B,Batterham P,Rutherford SL,Hoffmann AA

更新日期：2003-11-11 00:00:00

abstract：:Combinatorial transcription codes generate the myriad of cell types during development and thus likely provide crucial insights into directed differentiation of stem cells to a specific cell type. The LIM complex composed of Isl1 and Lhx3 directs the specification of spinal motor neurons (MNs) in embryos. Here, we rep...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lee S,Cuvillier JM,Lee B,Shen R,Lee JW,Lee SK

更新日期：2012-02-28 00:00:00

abstract：:Retrotransposons and retroviruses integrate nonrandomly into eukaryotic genomes. For the yeast retrotransposon Ty5, integration preferentially occurs within domains of heterochromatin. Targeting to these locations is determined by interactions between an amino acid sequence motif at the C terminus of Ty5 integrase (IN...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhu Y,Dai J,Fuerst PG,Voytas DF

更新日期：2003-05-13 00:00:00

abstract：:Preterm birth (PTB) complications are the leading cause of long-term morbidity and mortality in children. By using whole blood samples, we integrated whole-genome sequencing (WGS), RNA sequencing (RNA-seq), and DNA methylation data for 270 PTB and 521 control families. We analyzed this combined dataset to identify gen...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Knijnenburg TA,Vockley JG,Chambwe N,Gibbs DL,Humphries C,Huddleston KC,Klein E,Kothiyal P,Tasseff R,Dhankani V,Bodian DL,Wong WSW,Glusman G,Mauldin DE,Miller M,Slagel J,Elasady S,Roach JC,Kramer R,Leinonen K,Linth

更新日期：2019-03-19 00:00:00