Abstract：
:The objective of the present study was to investigate the presence of nine bacterial species in root-filled teeth associated with periapical lesions using a polymerase chain reaction analysis and to correlate these species with clinical features of the cases. DNA was extracted from 45 canal samples of root-filled teeth with periapical lesions. A PCR assay using species-specific primers of 16S rDNA and the downstream intergenic spacer region was used for microbial detection. Enterococcus faecalis was the most prevalent species, detected in 77.8% of the study teeth, followed by Peptostreptococcus micros, detected in 51.1%. Porphyromonas gingivalis, Porphyromonas endodontalis, Prevotella intermedia, and Prevotella nigrescens were detected in 35.6%, 22.2%, 11.1%, and 11.1% of the sampled teeth, respectively. Moreover, PCR detected Filifactor alocis in 26.7%, Treponema denticola in 24.4%, and Tannerella forsythia in 4.4% of the samples. T. denticola and P. micros were statistically associated with tenderness to percussion (p < 0.05). P. nigrescens was associated with the presence of spontaneous pain and abscess (p < 0.05). P. endodontalis and P. nigrescens were associated with purulent exudates (p < 0.05). Synergistic relationship was also observed between some species. The results of this study indicated that E. faecalis was the most frequently identified test species by PCR in teeth with failing endodontic treatment.
journal_name
J Endodjournal_title
Journal of endodonticsauthors
Gomes BP,Pinheiro ET,Jacinto RC,Zaia AA,Ferraz CC,Souza-Filho FJdoi
10.1016/j.joen.2008.01.016subject
Has Abstractpub_date
2008-05-01 00:00:00pages
537-40issue
5eissn
0099-2399issn
1878-3554pii
S0099-2399(08)00056-3journal_volume
34pub_type
杂志文章abstract：INTRODUCTION:Neuropeptides play an important role in inflammation and repair and have been implicated in mediating angiogenesis. Pulp fibroblasts express neuropeptide receptors, and the aim of this research was to investigate whether neuropeptides could regulate angiogenic growth factor expression in vitro METHODS:An ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2009.03.005
更新日期：2009-06-01 00:00:00
abstract：:Inflammatory myofibroblastic tumors (IMTs) are rare. IMTs of the head and neck occur in all age groups, from neonates to old age, with the highest incidence occurring in childhood and early adulthood. An IMT has been defined as a histologically distinctive lesion of uncertain behavior. This article describes an unusua...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2015.09.006
更新日期：2015-12-01 00:00:00
abstract：:The purpose of this study was to compare the flow of gutta-percha and Resilon (RealSeal; SybronEndo, Orange, CA) into lateral grooves and depressions in the apical 7 mm of a root canal by using warm vertical compaction. A split-tooth model was constructed with lateral grooves and depressions prepared at 1, 3, 5, and 7...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2007.02.017
更新日期：2007-06-01 00:00:00
abstract：INTRODUCTION:This study examined the effect of a new bioactive dentin substitute material (Biodentine) on the viability of human gingival fibroblasts. METHODS:Biodentine, White ProRoot mineral trioxide aggregate (MTA), and glass ionomer cement were evaluated. Human gingival fibroblasts were incubated for 1, 3, and 7 d...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2012.11.026
更新日期：2013-04-01 00:00:00
abstract：INTRODUCTION:Platelet-rich plasma (PRP) has been widely used in regenerative dentistry for over 2 decades. Nevertheless, previous studies have shown that its growth factor content is released over a short time period, and the application of anticoagulants limits its regenerative potential. Therefore, a second-generatio...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2019.04.002
更新日期：2019-08-01 00:00:00
abstract：:The purpose of this study was to evaluate the ultramorphological and chemical changes in Er,Cr:YSGG laser-treated radicular dentin in comparison with two different irrigation regimes. Extracted human premolars were assigned into three experimental groups. Group 1: After each file size, root canals were irrigated with ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2005.08.005
更新日期：2006-05-01 00:00:00
abstract：:Conventional and surgical endodontic management were performed on a two-rooted maxillary central incisor with a periapical lesion. Anatomical complexity of the tooth lead to perforation in the distal root during conventional treatment, thus a surgical approach was taken. A 4-month postoperative radiograph shows repair...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1097/00004770-200306000-00011
更新日期：2003-06-01 00:00:00
abstract：INTRODUCTION:Several bioactive bioceramics with osteoconductive effects can inhibit osteoclast formation and bone resorption in vitro, but the exact underlying mechanism has not been fully elucidated. Bioaggregate (BA), a novel calcium silicate nanoparticulate bioceramic, significantly induces bone and periodontal rege...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2015.05.018
更新日期：2015-09-01 00:00:00
abstract：:Endodontic fillings were challenged with bacterial ingress in mandibular premolars of 4 beagle dogs. Groups 1, 2, and 3 (n = 9), had canals filled with gutta-percha and sealer, gutta-percha alone, and sealer alone, respectively. After 2 wk, pulp chambers were inoculated with plaque. Group 4 (n = 9) and group 5 (n = 5)...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(06)81120-9
更新日期：1997-09-01 00:00:00
abstract：PURPOSE:To evaluate in vitro the solubility of the Epiphany endodontic filling material Epiphany (Pentron Clinical Technologies, Wallingford, CT) prepared with its resinous solvent. METHODS:The specimens were prepared in the following experimental conditions: (1) GI, epiphany without photoactivation; (2) GII, Epiphany...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2009.01.021
更新日期：2009-05-01 00:00:00
abstract：:The purposes of this study were to determine quantitatively the amount of debris and irrigant forced in an apical direction, the frequency of apical plug development, and the time required to prepare canals when a step-back technique using K-files was compared with the .04 Taper system. Sixty-nine extracted teeth with...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(98)80206-9
更新日期：1998-01-01 00:00:00
abstract：:A case report is presented describing the treatment for endemic dental fluorosis. Six maxillary anterior teeth were treated, three with an acid-bleach combination technique and three with an acid technique. No clinical differences was noted in treatment time or esthetic results. ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(06)81703-6
更新日期：1991-07-01 00:00:00
abstract：INTRODUCTION:The aim of this retrospective study was to evaluate the outcome of endodontic microsurgery and to examine prognostic factors related to healing. METHODS:The clinical records of all patients who had undergone endodontic microsurgery from 1997-2003 at the National Dental Centre of Singapore were examined. T...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2014.04.005
更新日期：2014-08-01 00:00:00
abstract：INTRODUCTION:The aim of this study was to evaluate the bond strength of different adhesive sealers on Resilon and gutta-percha. METHODS:Pellets of gutta-percha and Resilon were embedded into test tubes. Small eyelets were attached to those surfaces with a 0.5-mm film of different root canal sealers. Real Seal, Real Se...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2010.01.004
更新日期：2010-05-01 00:00:00
abstract：INTRODUCTION:Concentrated growth factor (CGF) is considered to be a natural biomaterial that is better than platelet-rich fibrin (PRF) in bone regeneration, but there is little information acquired in regenerative endodontics. Therefore, the purpose of this study was to evaluate their effects on the proliferation, migr...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2018.03.006
更新日期：2018-06-01 00:00:00
abstract：:Irreversibly inflamed pulp (IIP) constitutes both a pathophysiologic and a diagnostic challenge. Gingival crevicular fluid (GCF) samples were obtained with Periopaper strips from IIP and adjacent and contralateral teeth for interleukin-8 (CXCL8) and tumor necrosis factor-alpha (TNF-alpha) measurements. Pain intensity ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2007.10.022
更新日期：2008-02-01 00:00:00
abstract：INTRODUCTION:Different single-file systems are available for endodontic treatment; however, comparative studies are scarce. Thus, the present study evaluated bacterial reduction promoted by 2 single-file systems: Wave One (Dentsply Maillefer, Ballaigues, Switzerland) and One Shape (Micromega, Besancon, France). METHOD...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2014.07.024
更新日期：2014-12-01 00:00:00
abstract：:Interleukin-10 receptor (IL-10R) expression in human, dental pulp, fibroblast cultures was investigated by using the reverse transcriptase-polymerase chain reaction (RT-PCR) technique. After exposure to lipopolysaccharide (LPS) from Prevotella intermedia, the IL-10R mRNA levels increased after 4 h, peaked at 7 h, and ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1097/00004770-200301000-00013
更新日期：2003-01-01 00:00:00
abstract：INTRODUCTION:The purpose of this study was to compare digital periapical and cone-beam computed tomography (CBCT) images to determine the number of canals in the mesiobuccal root of maxillary molars and to compare these counts with micro computed tomography (μCT), which was also used to determine canal configuration. ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2013.01.010
更新日期：2013-07-01 00:00:00
abstract：INTRODUCTION:This review aimed to compare the kinematic effect of nickel-titanium instruments with reciprocating and continuous rotation motion for cyclic fatigue resistance, shaping ability, apical debris extrusion, and dentinal defects or cracks. METHOD:Articles were selected for inclusion in this review if they ful...
journal_title：Journal of endodontics
pub_type： 杂志文章,评审
doi：10.1016/j.joen.2016.04.002
更新日期：2016-07-01 00:00:00
abstract：:The aim of this study was to describe a guided endodontic technique that facilitates access to root canals of molars presenting with pulp calcifications. A 61-year-old woman presented to our service with pain in the upper left molar region. The second and third left molars showed signs of apical periodontitis confirme...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2018.02.009
更新日期：2018-05-01 00:00:00
abstract：INTRODUCTION:The aim of this study was to evaluate the effect of a range of alkaline pH values on the push-out strength of white mineral trioxide aggregate (WMTA). METHODS:The standardized lumens of root slices prepared from extracted single-rooted human teeth were filled with white ProRoot MTA. The specimens were the...
journal_title：Journal of endodontics
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.joen.2010.08.022
更新日期：2010-11-01 00:00:00
abstract：:A previous study showed that the ferret canine is an appropriate model for inducing periapical lesions. Ferret canines were used in this study to study changes in periapical lesions after root canal treatment (RCT). After periapical pathosis was induced in the maxillary and mandibular canines of six ferrets, RCT was p...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(06)80506-6
更新日期：1993-03-01 00:00:00
abstract：:The purpose of this study was to determine the influence of operator experience on the ability to locate and fill extra canals in maxillary first and second molars in vivo. The number of canals located and filled from maxillary first and second molars was recorded from three endodontic residents during the first 6 mon...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2005.11.005
更新日期：2007-01-01 00:00:00
abstract：:An examination of 5616 endodontically treated and retreated maxillary first and second molars was made in an attempt to determine the percentage of MB2 canals that could be located routinely, and evaluate if there were any significant differences between initial treatments and retreatments. The teeth examined were 357...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1097/01.don.0000140581.38492.8b
更新日期：2005-04-01 00:00:00
abstract：:Ten extracted human maxillary first molars were selected with a variety of root curvatures. The distofacial roots were resected and the mesiofacial canals were instrumented to a size #25 K file. Three techniques were used to introduce Ca(OH)2 paste into the instrumented canals. These techniques were evaluated for thei...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/s0099-2399(06)81220-3
更新日期：1992-08-01 00:00:00
abstract：:Inflammation of pulp tissue appears as a consequence of caries progression. Its main characteristic is inflamed infiltrate whose cells contain lymphocytes. CD44 is a widely expressed adhesion molecule present in several body cells such as leukocytes and parenchymatous cells, including endothelial cells, epithelial cel...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2007.05.022
更新日期：2007-10-01 00:00:00
abstract：:Formocresol pulpotomy (FP) in the United States is most frequently used to treat asymptomatic caries near the pulp in primary teeth. Indirect pulp therapy (IPT) is also indicated and has a significantly higher long-term success. Pulpotomy is thought to be indicated for primary teeth with carious pulp exposures, but re...
journal_title：Journal of endodontics
pub_type： 杂志文章,评审
doi：10.1016/j.joen.2008.02.033
更新日期：2008-07-01 00:00:00
abstract：:This study was aimed at comparing the cultivable microorganisms in canals with periapical radiolucencies with exposed and unexposed pulp space. Microbiological samples were taken and analyzed from 45 canals with exposed pulp space, and 43 canals with unexposed pulp space. The canal contents were analyzed by aerobic/an...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1097/01.don.0000148149.27636.9c
更新日期：2005-06-01 00:00:00
abstract：INTRODUCTION:This study aimed to investigate dental pulp responses to novel bioactive glass (BG) pulp capping materials after direct pulp capping in vivo. METHODS:Novel BG pulp capping materials are composed of powder and fluid. The powder is BG (82.36% SiO2, 15.36% CaO, and 2.28% P2O5), and the fluid is provided in 2...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2017.03.011
更新日期：2017-10-01 00:00:00
