Abstract:
INTRODUCTION:Platelet-rich plasma (PRP) has been widely used in regenerative dentistry for over 2 decades. Nevertheless, previous studies have shown that its growth factor content is released over a short time period, and the application of anticoagulants limits its regenerative potential. Therefore, a second-generation platelet concentrate (liquid platelet-rich fibrin [PRF]) was developed without the use of anticoagulants and with shorter centrifugation times. The purpose of the present study was to compare the cellular regenerative activity of human dental pulp cells (hDPCs) when cultured with either liquid PRF or traditional PRP. METHODS:The regenerative potential of hDPCs isolated from healthy human third molars (18-22 years, n = 5) was investigated in both normal and inflammatorylike conditions (lipopolysaccharide [LPS]) and assessed for their potential for dentin repair. The effects of liquid PRF and PRP were assessed for cellular migration, proliferation, and odontoblastic differentiation using a transwell assay, scratch assay, proliferation assay, alkaline phosphatase assay, alizarin red staining, and real-time polymerase chain reaction for genes encoding collagen type 1 alpha 1, dentin sialophosphoprotein, and dentin matrix protein 1, respectively. The effects of both platelet concentrates were also assessed for their ability to influence nuclear translocation of nuclear factor kappa B (p65) by immunofluorescence, and reverse-transcription polymerase chain reaction for genes encoding interleukin-1β, tumor necrosis factor alpha, and nuclear factor kappa B (p65) during an inflammatory condition. RESULTS:Both PRP and liquid PRF increased the migration and proliferation of hDPCs when compared with the control group, whereas liquid PRF showed a notable significant increase in migration when compared with PRP. Furthermore, liquid PRF induced significantly greater alkaline phosphatase activity, alizarin red staining, and a messenger RNA expression of genes encoding collagen type 1 alpha 1, dentin sialophosphoprotein, and dentin matrix protein 1 when compared with PRP. When hDPCs were cultured with LPS to stimulate an inflammatory environment, a marked decrease in dentin-related repair was observed. When liquid PRF was cultured within this inflammatory environment, the reduced regenerative potential in this LPS-produced environment was significantly and markedly improved, facilitating hDPC regeneration. The messenger RNA expression of inflammatory markers including tumor necrosis factor alpha, interleukin-1β, and p65 were all significantly decreased in the presence of liquid PRF, and, furthermore, liquid PRF also inhibited the transport of p65 to the nucleus in hDPCs (suggesting a reduced inflammatory condition). CONCLUSIONS:The findings from the present study suggest that liquid PRF promoted greater regeneration potential of hDPCs when compared with traditional PRP. Furthermore, liquid PRF also attenuated the inflammatory condition created by LPS and maintained a supportive regenerative ability for the stimulation of odontoblastic differentiation and reparative dentin in hDPCs.
journal_name
J Endodjournal_title
Journal of endodonticsauthors
Chai J,Jin R,Yuan G,Kanter V,Miron RJ,Zhang Ydoi
10.1016/j.joen.2019.04.002subject
Has Abstractpub_date
2019-08-01 00:00:00pages
1000-1008issue
8eissn
0099-2399issn
1878-3554pii
S0099-2399(19)30291-2journal_volume
45pub_type
杂志文章abstract:INTRODUCTION:This study evaluated the influence of glide path on canal curvature and axis modification after instrumentation with WaveOne Primary reciprocating files. METHODS:Thirty ISO 15, 0.02 taper Endo Training Blocks were used. In group 1, glide path was created with PathFile 1, 2, and 3 at working length, wherea...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2011.09.030
更新日期:2012-01-01 00:00:00
abstract::The dislocation resistance of root fillings created with MetaSEAL, a self-adhesive 4-META-containing methacrylate resin-based sealer, was evaluated. Forty-six incisors were cleaned and shaped using NaOCl and EDTA as irrigants. They were filled with gutta-percha/MetaSEAL or gutta-percha/AH Plus sealer using either a si...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2008.03.028
更新日期:2008-07-01 00:00:00
abstract:INTRODUCTION:Idiopathic bone cavity (IBC) is an uncommon bone lesion that usually affects youngsters as an unilocular radiolucency with predilection for the posterior mandible. Because the lesion is frequently located in proximity to the adjacent teeth, chronic apical periodontitis is commonly included as a differentia...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2020.11.004
更新日期:2021-02-01 00:00:00
abstract:INTRODUCTION:The purpose of this in vivo study was to evaluate the accuracy of small-volume cone-beam computed tomographic (CBCT) imaging compared with conventional periapical radiography (CPR) in the diagnosis of vertical root fractures (VRFs) using exploratory surgery as the reference standard. METHODS:Eighty-two de...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2020.04.015
更新日期:2020-08-01 00:00:00
abstract::A case study is presented that described an acute flare-up of a tooth following a vital bleaching procedure. The case illustrates (a) the importance of assessing the pulpal and periradicular status of a tooth prior to any vital bleaching procedure; and (b) the alteration of the local adaptation syndrome by the bleachi...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/S0099-2399(06)80488-7
更新日期:1992-07-01 00:00:00
abstract:INTRODUCTION:Apical root resection is a biologically essential component in endodontic microsurgery. However, because it reduces the total root length and supported root surface, it changes the biomechanical response of the tooth. The purpose of this study was to analyze the biomechanical effect of apical root resectio...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2014.03.006
更新日期:2014-09-01 00:00:00
abstract:INTRODUCTION:The aim was to assess the association of inducible costimulator (ICOS) and ICOS ligand with bone destruction in apical periodontitis (AP). METHODS:Specimens from patients presenting with AP were obtained during apicoectomy and subjected to histopathologic analysis and molecular assessment of ICOS/ICOS lig...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2019.03.018
更新日期:2019-07-01 00:00:00
abstract::Although many options are proposed for the treatment of cracked posterior teeth, most treatment decisions are not evidence based. Thus, considerable individual variation can occur regarding treatment recommendations for the same scenario. To our knowledge, there are no studies in the literature assessing practitioners...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2017.03.029
更新日期:2017-09-01 00:00:00
abstract:INTRODUCTION:It has been reported that integrin-α5 (ITGA5) activity is related to cell proliferation, differentiation, migration, and organ development. However, the involvement of ITGA5 in the biological functions of human dental pulp stem cells (hDPSCs) has not been explored. The aim of this study was to investigate ...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2013.08.011
更新日期:2014-02-01 00:00:00
abstract::Teeth are vital sensory organs that contribute to our daily activities of living. Unfortunately, teeth can be lost for several reasons including trauma, caries, and periodontal disease. Although dental trauma injuries and caries are more frequently encountered in a younger population, tooth loss because of periodontal...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2019.05.014
更新日期:2019-12-01 00:00:00
abstract::Primary intraosseous salivary gland tumors are rare, with mucopidermoid carcinoma being the most frequent histotype. The authors present a case of adenoid cystic carcinoma, located in the mandibular incisor region, associated with pain. Endodontic treatment resulted in increased pain and progressive mandibular expansi...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1097/00004770-200012000-00026
更新日期:2000-12-01 00:00:00
abstract:INTRODUCTION:In a clinical situation, an apically resected tooth is often accompanied by a varying degree of periodontal bone loss. The purpose of this study was to assess the influence of apical root resection combined with periodontal bone loss on the biomechanical response of a single-rooted tooth. METHODS:A basic ...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2014.11.011
更新日期:2015-03-01 00:00:00
abstract::The treatment of an undeveloped (immature) tooth with a carious exposure has been reported extensively in the literature. The main objective of treatment is to provide vital pulp therapy to allow continued development of root dentin in the hope that the root will attain full maturity. A case report is presented that i...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1097/00004770-200311000-00022
更新日期:2003-11-01 00:00:00
abstract::Stem cell therapy is a potential strategy to regenerate the dentin-pulp complex, enabling the conservation and restoration of functional teeth. We assessed the efficacy and safety of pulp stem cell transplantation as a prelude before the initiation of clinical trials. Granulocyte-colony stimulating factor (G-CSF) indu...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2014.01.020
更新日期:2014-04-01 00:00:00
abstract:INTRODUCTION:This ex vivo study compared the disinfection and shaping abilities of 2 preparation protocols in C-shaped canals. METHODS:Mandibular second molars with type I C-shaped canals were pair matched based on micro-computed tomographic (micro-CT) analysis and distributed into 2 groups. The canals were contaminat...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2020.11.007
更新日期:2020-11-18 00:00:00
abstract:INTRODUCTION:Stem cells from the apical papilla (SCAPs) possess strong odonto/osteogenic differentiation potential. This study investigated the effect of cyclic adenosine monophosphate (cAMP) on odonto/osteogenic differentiation of SCAPs and the underlining interplay between cAMP and transforming growth factor beta 1 (...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2018.10.008
更新日期:2019-02-01 00:00:00
abstract::The mesiobuccal roots of 50 randomly selected maxillary first molars were examined to assess the incidence and position of the canal isthmus. Transverse serial sections of the apical 6 mm of each root were prepared in 1-mm increments. The apical side of each section was stained with methylene blue dye, viewed with a s...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/s0099-2399(06)80975-1
更新日期:1995-07-01 00:00:00
abstract:INTRODUCTION:Sphingosine-1-phosphate receptor 1 (S1P1) is crucial for regulation of immunity and bone metabolism. This study aimed to investigate the expression of S1P1 in rat periapical lesions and its relationship with receptor activator of nuclear factor kappa B ligand (RANKL) and regulatory T (Treg) cells. METHODS...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2014.10.010
更新日期:2015-04-01 00:00:00
abstract:INTRODUCTION:The goal of replantation after dental avulsion is to maintain the tooth in its socket. Presence of vital periodontal ligament cells on the root surface of the replanted tooth and the immunologic status of the patient are factors that protect against root resorption. It is known that dental constituents pla...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2016.09.020
更新日期:2017-01-01 00:00:00
abstract:INTRODUCTION:The objective of this study was to investigate the association between chronic apical periodontitis (CAP) and low-birth-weight preterm births (LBWPB). METHODS:Sixty-three women in postpartum period were included in this case-control study. The case group consisted of mothers of LBWPB infants (n = 33), and...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2014.11.018
更新日期:2015-03-01 00:00:00
abstract::A descriptive, cross sectional survey was developed to determine the preferences of endodontists when prescribing analgesics. Eleven clinical scenarios describing common endodontic diagnoses or procedures with specified severity of pain were provided. A survey was sent to 310 AAE members and 63 responded, providing a ...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2006.07.015
更新日期:2006-12-01 00:00:00
abstract:INTRODUCTION:Difficult handling, long setting time, and potential discoloration are important drawbacks of white mineral trioxide aggregate (WMTA). The development of Biodentine, a recently developed calcium silicate-based material (CSM), has overcome some of these shortcomings; however, there are no available data on ...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2012.12.021
更新日期:2013-04-01 00:00:00
abstract::Biofilm mode of growth is a strategy in microorganisms to survive harsh growth conditions. Although previous studies have established the ability of Enterococcus faecalis to survive postendodontic environmental conditions, the effect of such conditions on the ultrastructural and physiochemical features of E. faecalis ...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1097/01.don.0000164855.98346.fc
更新日期:2005-12-01 00:00:00
abstract:INTRODUCTION:The purpose of the present study was to evaluate the thickness and the anatomic characteristics of the Schneiderian membrane and cortical bone using limited cone beam computed tomography (CBCT) scannning in patients referred for planning of apical surgery of maxillary molars. METHODS:This controlled study...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2011.09.023
更新日期:2012-01-01 00:00:00
abstract::In this study, cell reaction after exposure to several dental cements was determined using a dentin barrier test device. The reaction of mouse fibroblasts grown on the "pulpal" side of a bovine dentin disk was determined after exposure to dental cements applied on the "cavity" side of the disk. For pretreatment, denti...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/s0099-2399(96)80285-8
更新日期:1996-03-01 00:00:00
abstract::In the present study, we used Fos expression as an index of nociceptive input to the spinal trigeminal nucleus after exposure of the coronal pulp tissue of maxillary right first molars and examined the effects of pretreatment with an opioid, a nonsteroidal anti-inflammatory drug or a local anesthetic before pulp expos...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1097/01.don.0000153844.61709.64
更新日期:2005-08-01 00:00:00
abstract:INTRODUCTION:The effect of endodontic involvement on tooth loss has not been quantified, so the present study aimed to assess this relationship after controlling for other relevant risk factors for tooth loss. METHODS:We analyzed data from 791 participants (18,798 teeth) in the Veterans Affairs Dental Longitudinal Stu...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2010.08.051
更新日期:2010-12-01 00:00:00
abstract:INTRODUCTION:This study evaluated the influence of cervical preflaring on the incidence of root dentin defects after root canal preparation. METHODS:Extracted human maxillary central incisors were selected and allocated to 1 control group and 12 experimental groups (n = 15). Teeth in the control group were left unprep...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2017.09.021
更新日期:2018-02-01 00:00:00
abstract:INTRODUCTION:The role of complement, especially through the C5a fragment, is well-known for the initiation of inflammation. Its involvement in regeneration has been shown more recently by the recruitment of mesenchymal stem cells. C5a can be produced locally by the pulp fibroblasts in response to injury or infection. T...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2017.02.016
更新日期:2017-07-01 00:00:00
abstract::Conventional and surgical endodontic management were performed on a two-rooted maxillary central incisor with a periapical lesion. Anatomical complexity of the tooth lead to perforation in the distal root during conventional treatment, thus a surgical approach was taken. A 4-month postoperative radiograph shows repair...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1097/00004770-200306000-00011
更新日期:2003-06-01 00:00:00